Adipokinetic hormone signaling regulates adult dehydration resistance in the migratory locust

Drought events have become more severe under climate change,and this can pose a major threat to the survival of various organisms.The molecular mechanisms involved in dehydration resistance are not well known.Here,adults of the migratory locust,Locusta migratoria,were subjected to food-mediated dehydration,and adipokinetic hormone(AKH)signaling was found to play a key role in regulating dehydration resistance.Specifically,dehydration shortened the lifespan,increased the body weight loss,and reduced the water loss rate in adult locusts.Global transcriptome profiles revealed variations in tissue-specific gene expression between dehydration-resistant locusts and normal locusts.Importantly,dehydration selection and exposure induced prominent expression of AKH genes in the retrocerebral complex of adult locusts.Furthermore,individual knockdown of AKH1,AKH2,or AKH receptor(AKHR)accelerated water loss and shortened the lifespan of adult locusts under dehydration conditions,and trehalose supplementation ameliorated the negative effects caused by interference with AKH or AKHR.These findings demonstrated that AKH/AKHR signaling-dependent trehalose metabolism plays a crucial role in regulating locust dehydration resistance and thus provide novel insights into the regulatory mechanism underlying drought resistance.

Oncologic outcomes with and without amniotic membranes in robotic-assisted radical prostatectomy:A propensity score matched analysis

Objective:Placement of human placenta derived grafts during robotic-assisted radical prostatectomy(RARP)hastens the return of continence and potency.The long-term impact on the oncologic outcomes remains to be investigated.Our objective was to determine the oncologic outcomes of patients with dehydrated human amnion chorion membrane(dHACM)at RARP compared to a matched cohort.Methods:In a referral centre,from August 2013 to October 2019,599 patients used dHACM in bilateral nerve-sparing RARP.We excluded patients with less than 12 months follow-up,simple prostatectomy,and unilateral nerve-sparing.Patients with dHACM(amnio group)were 529,and were propensity score matched 1:1 to 2465 patients without dHACM(non-amnio group)and a minimum follow-up of 36 months.At the time of RARP,dHACM was placed around the neurovascular bundle in the amnio group.Continuous and categorical variables in matched groups was tested by two-sample Kolmogorov-Smirnov test and Fisher's exact test respectively.Outcomes measured were biochemical recurrence(BCR),adjuvant and salvage therapy rates.Results:Propensity score matching resulted in two groups of 444 patients.Cumulative incidence functions for BCR did not show a difference between the groups(p=0.3).Patients in the non-amnio group required salvage therapy more frequently than the amnio group,particularly after partial nerve-sparing RARP(6.3%vs.2.3%,p=0.001).Limitations are the absence of prospective randomization.Conclusion:The data suggest that using dHACM does not have a negative impact on BCR in patients.Outcomes of cancer specific and overall survival will require follow-up study to increase our understanding of these grafts’impact on prostate cancer biology.

Study on the effect of four kinds of raw materials in hypertonic dehydration cell model

It aims to investigate the protective effects of sodium hyaluronate,panthenol,Portulaca oleracea L.and Calendula officinalis L.on hyperosmotic dehydration-induced injury of human immortalized keratinocytes(HaCaT).The safety mass concentrations of four raw materials were screened by detecting cell viability,and the secretion of hyaluronic acid(HA)was determined using the ELISA method.The expression of HaCaT barrier function related genes(OVOL1,EREG,TGM1,TGM2,IVL,IRF6,THBS1,CASP14)was detected at the mRNA level to explore the regulatory effect of four raw materials on these genes.The results demonstrate that pretreatment with the four kinds of raw materials could increase the cell viability after hyperosmotic dehydration,promote the secretion of HA,and improve the expression of barrier function related genes after hyperosmotic dehydration,among which panthenol and Calendula officinalis L.are better.The results show that the four raw materials have a certain protective effect on the hyperosmotic dehydration cell model,which provides data support for its application in cosmetics.

Hydroelectrolytic and Energetic Replenisher in Horses Undergoing Marcha Training

The study aimed to assess the clinical, laboratory, and blood gas analysis of horses undergoing Marcha training and the effects of voluntary ingestion of hydroelectrolytic and energy replenishers after exercise. Eight horses of both genders aged between 5 and 10 years, were included in the study. The exercise consisted of a 10-min warm-up followed by 45 min uninterrupted Marcha on a flat dirt track in the morning. After exercise, the horses received one of the following treatments: Drinking water (control group);Hydroelectrolytic and energy replenisher containing sodium chloride, potassium chloride, calcium acetate, magnesium chloride, sodium citrate, dextrose, maltodextrin, and sucrose in three different concentrations (Replenishers A, B, and C). The horses were distributed across the four treatments in a 4 × 4 Latin Squares design using a Split-plot system with 48-hr intervals. Clinical and laboratory evaluations were conducted at four time points: T0 - 5 min before exercise;T1 - up to 5 min after exercise;T2 - 2 hr after starting treatment;and T4 - 4 hr after beginning treatment. Concentrations of urea, creatinine, lactate, phosphorus, and ionized calcium significantly changed after exercise. An increase in blood pH and a decrease in chloride concentrations were observed when replenishers B and C were offered after exercise. The replacements were ingested spontaneously by the animals in a volume greater than that of the control group (water). Replacement B was the most ingested by the animals, demonstrating its greatest potential.

Quantification of In Vivo Epidermal Keratinocyte Architecture Associated with the Signs of Skin Aging and the Skin Benefit Evaluation by Application of Galactomyces Ferment Filtrate (Pitera)-Containing Skin Care Product

Background: Aged skin exhibits visual alterations such as wrinkles, rough texture, pore dilation, and dull skin tone, as well as physiological aging, namely, decreased hydration and increased transepidermal water loss (TEWL). Recent advances in coherence tomography have also revealed that skin aging affects in vivo epidermal keratinocyte architecture. However, the interconnectivity between spatial architectural aging and visual/physiological aging parameters remains largely unknown. Purpose: To elucidate whether the tomographic keratinocyte architectural aging is correlated with visual and physiological skin aging parameters and to quantitatively evaluate the improvements of the architectural, visual, and physiological aging parameters by the daily treatment of the skin care formula containing Galactomyces Ferment Filtrate (GFF, 8X PiteraTM). Method: We measured the in vivo keratinocyte cellular architecture with two-photon stereoscopic tomography obtaining by-layer epidermal section images in 78 Asian females of various ages. Visual aging parameters were analyzed using a portable image capture system. Hydration and TEWL were also assessed. The anti-aging effects of GFF-containing skin moisturizer (SK-II LXP CreamTM) were also examined in two studies after twice-daily application for 2 (N = 35) and 4 (N = 32) weeks. Results: As for the keratinocyte cellular architecture, skin aging was significantly associated with decreased cell density and increased cell uniformity. These architectural aging parameters were significantly correlated with visual and physiological aging parameters, namely, rough texture, wrinkles, pore dilation, dull skin tone, dehydration, and increased TEWL. The strong interconnectivity allowed us to develop formulae to estimate the keratinocyte architecture from visual aging parameters. Moreover, twice-daily application of SK-II significantly improved the keratinocyte architecture associated with multiple skin aging visual and physiological parameters. Conclusion: Skin aging is a process involving mutual interconnections among epidermal keratinocyte cellular architecture, visual, and physiological parameters. The GFF-containing moisturizer SK-II effectively improves spatial architecture of keratinocytes in epidermis and these evaluated skin aging parameters in a new trajectory over the course of treatment. .

Time-resolved multiomics analysis of the genetic regulation of maize kernel moisture

Maize kernel moisture content(KMC)at harvest greatly affects mechanical harvesting,transport and storage.KMC is correlated with kernel dehydration rate(KDR)before and after physiological maturity.KMC and KDR are complex traits governed by multiple quantitative trait loci(QTL).Their genetic architecture is incompletely understood.We used a multiomics integration approach with an association panel to identify genes influencing KMC and KDR.A genome-wide association study using time-series KMC data from 7 to 70 days after pollination and their transformed KDR data revealed respectively 98and 279 loci significantly associated with KMC and KDR.Time-series transcriptome and proteome datasets were generated to construct KMC correlation networks,from which respectively 3111 and 759 module genes and proteins were identified as highly associated with KMC.Integrating multiomics analysis,several promising candidate genes for KMC and KDR,including Zm00001d047799 and Zm00001d035920,were identified.Further mutant experiments showed that Zm00001d047799,a gene encoding heat shock 70 kDa protein 5,reduced KMC in the late stage of kernel development.Our study provides resources for the identification of candidate genes influencing maize KMC and KDR,shedding light on the genetic architecture of dynamic changes in maize KMC.

A thermodynamic-based model for modeling thermo-elastoplastic behaviors of saturated clayey soils considering bound water dehydration

The non-isothermal deformation of soft mudrocks or clay soils is one of the most critical issues in energy and environmental related geotechnics.Clay-related geomaterials hold complex microstructure and mineral composition,which brings difficulty in investigating their thermo-mechanical behaviors.Previous studies pay little attention to the difference between a thermal plastic strain and the strain from clay dehydration.In this study,a new constitutive model is proposed for describing the thermoelastoplastic behaviors of clayey soils under water-saturated condition.The effect of temperature variation and mechanical loading on elastoplastic strains and dehydration are investigated.The thermodynamics laws and the unconventional plasticity are applied to quantify the thermo-mechanical behavior.The irreversible strain is captured by using Cam-Clay plasticity and subloading yield surface concept.The dehydration strain is described by utilizing a novel method based on generalized thermodynamics approach and Helmholtz free energy function.The internal variables,and the first and second laws of thermodynamics are applied in the model.The hardening rule is established by implementing the laws of physical conservation,energy dissipation,and plastic flow.The proposed model is validated using specially designed thermal consolidation tests on laboratory prepared heavily consolidated clayey soils and some published data of clayey soils with different geological origins.

Experimental and numerical studies of Ca(OH)_(2)/CaO dehydration process in a fixed-bed reactor for thermochemical energy storage

The Ca(OH)_(2)/CaO thermochemical energy storage(TCES)system based on calcium looping has received extensive attention owing to its high energy storage density,prolonged energy storage time,and environmental friendliness.The heat storage process of the Ca(OH)_(2)/CaO TCES system in a mixed heating reactor was evaluated in this study,by employing a combination of direct and indirect heating modes.The dehydration process was studied experimentally,and a numerical model was established and verified based on the experimental results.The dehydration behavior of 500 g of Ca(OH)_(2) powder was investigated in a fixed-bed reactor with mixed heating.The experimental and simulation results indicated that mixed heating causes combined centripetal and horizontal propulsion.Heat input is the main limiting factor in the heat storage process,because the radial advance of the reaction is hindered by the low thermal conductivity of the solid reactant particles.Heat transmission partitions were added to enhance the performance of the reactor.The performance of the modified reactor was compared with that of a conventional reactor.The radial heat transmission partitions in the modified reactor effectively enhance the energy storage rate and reduce the reaction time by 59.5%compared with the reactor without partitions.

Role of Calcination Temperature on Isosorbide Production from Sorbitol Dehydration over the Catalyst Derived from Ce(IV)Sulfate

Isosorbide is a multi-purpose chemical that can be produced from renewable resources.Specifically,it has been investigated as a replacement for toxic bisphenol A(BPA)in the production of polycarbonate(PC).In this study,the synthesis of isosorbide by sorbitol dehydration using a cerium-based catalyst derived from calcined cerium(IV)sulfate(300°C,400°C,450°C,500°C,and 650°C)was investigated.The reaction occurred in a high-pressure reactor containing nitrogen gas.Advanced instrumental techniques were applied to analyze the characteristics of the calcined catalyst.The results showed that the calcined catalysts demonstrated different crystalline structures and sulfate species at different temperatures.However,the acidic properties(strength and amount)of the catalyst did not change with the calcination temperature.The cerium(IV)sulfate calcined at 400°C exhibited the best catalytic performance,achieving the highest isosorbide yield(55.7%)and complete conversion of sorbitol at 180°C,20 bar of N2,and 6 h using CeSO-400.The presence of a sulfate group on the catalyst was the most important factor in determining the catalytic performance of sorbitol dehydration to isosorbide.This work suggests that CeSO-400 catalysts may play an important role in reducing reaction conditions.

Effects of a Dehydrating Agent on Dry Matter Accumulation and Nutritional Quality of Different Maize Varieties

The high water content of corn grain at harvest is a challenge in Northeast China,where the growing season is short.Using a dehydrating agent before harvest can help corn seeds dehydrate quickly.The dry matter accumulation and nutrient quality of maize were systematically studied by field experiments and instrumental analysis using maize varieties of different maturities as test materials.The results showed that the accumulation of dry matter was enhanced by an increased dosage of a dehydrating agent.When the dehydrating agent dosage reached 1800 mL•hm-2,the dry matter accumulation of early-maturing varieties increased by 24.1 g,and the water content decreased by 8.08%.Different maize varieties were treated with the same dose;early-maturing varieties showed significant effects on grain dry matter accumulation,and kernel dry matter accumulation increased by 7%.The effects of different doses on grain dehydration were obvious,and the effects on different maize varieties varied.Medium-ripening maize varieties showed the most significant effect,with a 19.5%reduction in water content.The effects of dehydrating agent doses on maize yield,grain nutrient quality and seed germination rate were not significant.Therefore,a dehydrating agent promoted the accumulation of dry matter in grain and accelerated the rapid dehydration.

Pediatric thermal burn injury:A retrospective analysis from pediatric care institutes,Eastern India

Objective:Pediatric burns pose difficult healthcare issues in underdeveloped nations.Due to the scarcity of pediatric burn care facilities and qualified burn‑care staff,patients are frequently referred from a rural hospital.This study explores the burn‑treatment approach that could be used in rural hospitals in the absence of a dedicated pediatric burn care facility.Materials and Methods:A retrospective analysis of clinical data of 199 children who received treatment for burn‑injury in an isolated general surgical ward.Wound debridement under anesthesia at the earliest was the cornerstone of the treatment plan for this cohort.Until the wounds healed,the treatment was repeated every alternate day.The patient’s demographic data,number of surgical dressings,length of hospital stay,and outcome were analyzed.Results:The mean age of the patients was 51.68±23.79 months.The male‑female ratio was 1.45:1.The survival rate was 99.00%.The disability rate was 4.52%.Univariate logistic regression identified the age group,total body surface area affected,depth of the wound,and time of intervention as statistically significant variables(P<0.05)predicting the disfigurement.Multivariate logistic regression identified the time intervals between surgical intervention and injury as an important predictor of morbidity.In addition,delayed presentation and delayed intervention led to a longer hospital stay,and more interventions than the early intervention cohort.Conclusions:Early debridement accelerates recovery and lessens the need for future skin grafts or contracture procedures.This approach can be used in rural hospitals as well because pediatric burns can be manageable in an isolated general ward.

Safety of Empagliflozin in Patients with Type 2 Diabetes Mellitus in Saudi Arabia: A Post-Authorisation Safety Study

Background: Empagliflozin, a sodium-glucose co-transporter-2 (SGLT2) inhibitor is used as a monotherapy or in combination for lowering the elevated blood glucose level in patients with type 2 diabetes mellitus (T2DM). It is often associated with certain adverse reactions (urinary tract infection (UTI), diabetes ketoacidosis (DKA), and genital infections). Thus, the Saudi Food and Drug Administration requested a post-authorisation safety study to monitor the safety of empagliflozin during the defined observation period. Methodology: The local, comparator, non-interventional, regulatory post-marketing study using “new user” design was conducted in patients with T2DM, treated with empagliflozin (10 or 25 mg) and dipeptidyl peptidase-4 (DPP-4) inhibitors (NCT03764631). Study was conducted from 2018 to 2020, wherein each patient was followed up for 12 months after the index period. Incidence and occurrence of DKA, severe UTIs, volume depletion and dehydration were observed along with metformin, insulin and treatment complexity status and adverse events in the index and Ramadan period. All data collected were analysed using descriptive statistics. Results: Among the 1502 patients enrolled (empagliflozin [n = 751] and DPP-4 inhibitors [n = 751]), 0.1% patients (n = 1) in each group and Conclusion: Empagliflozin was well tolerated over a period of 12 months, with no safety concerns and a favourable benefit/risk ratio.

Expanded Follicle-Sulcus-Crack Complex Is an Early Warning Sign of Facial Skin Aging: Improvement by Application of Galactomyces Ferment Filtrate-Containing Skin Product

Background: Wrinkles, pigmented spots, and roughness are representative parameters reflecting facial skin aging. Sulci cutis connecting to follicular orifices frequently form perifollicular cracks, which join together adjacent sulci. This follicle-sulcus-crack complex (FSCC) is exacerbated by dehydration. Purpose: Although dehydration is associated with facial skin aging, FSCC’s implications in facial skin aging remain unresolved. Method: We measured facial skin parameters in 1999 and 2010 in 108 Japanese women, and in 2006 and 2016 in 182 Chinese women. We addressed whether expansion of FSCC is associated with other skin aging parameters. We also examined whether skin moisturizer can reverse the expanded FSCC. Results: In both clinical studies, FSCC severity as well as wrinkles, spots, and roughness significantly deteriorated with age. Notably, FSCC significantly increased as early as in subjects in their 20s, whereas wrinkles, spots, and roughness became conspicuous in those in their 40s or older. Moreover, subjects with more severe FSCC in the initial measurement exhibited significantly worse wrinkles, spots, and roughness in the second measurement a decade later. Dehydration was significantly correlated with expanded FSCC. Furthermore, FSCC expansion was reversed after appropriate moisturization by daily application of Galactomyces ferment filtrate (GFF)-containing skincare product (SK-II Skin Power Cream) for 4 weeks in 37 women. Conclusion: The expanded FSCC is likely to be an early indicator of facial skin aging. Appropriate moisturization may reduce FSCC formation and decelerate facial skin aging.

Non-isothermal kinetic analysis of thermal dehydration of La_2(CO_3)_3·3.4H_2O in air

The single phase La2（CO3）3·3.4H2 O was synthesized by hydrothermal method. The thermal decomposition and intermediates and final solid products of La2（CO3）3·3.4H2O from 30 to 1000 °C were characterized by XRD, FTIR and DTA-TG. The kinetics of dehydration of La2（CO3）3·3.4H2O in the temperature range of 30-366 °C was investigated under non-isothermal conditions. Flynn-Wall-Ozawa and Friedman isoconversion methods were used to calculate the activation energy and analyze the reaction steps; multivariate non-linear regression program was applied to determine the most probable mechanism and the kinetic parameters. The results show that the thermal dehydration of La2（CO3）3·3.4H2O is a kind of three-step competitive reaction, and controlled by an n-order initial reaction followed by n-order competitive reaction（FnFnFn model）. The activation energy matching with the most probable model is close to value obtained by Friedman method. The fitting curves match the original TG-DTG curves very well.

Three-stage Transformation of Chlorophyll Transient Fluorescence Pattern Under Sustained Dehydration and the Discovery of Critical Water Content in Seaweeds

The chlorophyll fluorescence kinetics of marine red alga Grateloupia turutunt Yamada, green alga Ulva pertusa Kjellm and brown alga Laminaria japonica Aresch during natural sustained dehydration were monitored and investigated. The pulse amplified modulation (PAM) system was used to analyze the distinct fluorescence parameters during thallus dehydration. Results proved that the fluorescence kinetics of different seaweed all showed three patterns of transformation with sustained water loss. These were: 1) peak kinetic pattern (at the early stage of dehydration fluorescence enhanced and quenched subsequently, representing a normal physiological state). 2) plateau kinetic pattern (with sustained water loss fluorescence enhanced continuously but quenching became slower, finally reaching its maximum). 3) Platform kinetic pattern (fluorescence fell and the shape of kinetic curve was similar to plateau kinetic pattern). A critical water content (CWC) could be found and defined as the percentage of water content just prior to the fluorescence drop and to be a significant physiological index for evaluation of plant drought tolerance. Once thallus water content became lower than this value the normal peak pattern can not be recovered even through rehydration, indicating an irreversible damage to the thylakoid membrane. The CWC value corresponding to different marine species were varied and negatively correlated with their desiccation tolerance, for example. Laminaria japonica had the highest CWC value (around 90%) and the lowest dehydration tolerance of the three. In addition, a fluorescence 'burst' was found only in red algae during rehydration. The different fluorescence parameters F-o, F-v and F-v, F-m were measured and compared during water loss. Both F-o and F-v increased in the first stage of dehydration but F-v/F-m. kept almost constant. So the immediate response of in vivo chlorophyll fluorescence to dehydration was an enhancement. Later with sustained dehydration F-o increased continuously while F-v decreased and tended to become smaller and smaller. The major changes in fluorescence (including fluorescence drop during dehydration and the burst during rehydration) were all attributed to the change in F-o instead of F-v This significance of F-o indicates that it is necessary to do more research on F-o as well as on its relationship with the state of thylakoid membrane.

Efficient production of 5-hydroxymethylfurfural from hexoses using solid acid SO_4^(2-)/In_2O_3-ATP in a biphasic system

A natural attapulgite （ATP）‐based catalyst, sulfated In2O3‐ATP （SO42-/In2O3‐ATP）, was obtained by an impregnation‐calcination method and was used to efficiently and selectively produce the useful platform chemical 5‐hydroxymethylfurfural （HMF） from hexoses. Some important reaction param‐eters were studied, revealing that Lewis and Br-nsted acid sites on SO42-/In2O3‐ATP catalyze glu‐cose isomerization and fructose dehydration. The yields of HMF from glucose and fructose were 40.2%and 46.2%, respectively, using the optimal conditions of 180℃ for 60 min with 10 wt%of solid acid catalyst in a mixture of γ‐valerolactone‐water （9：1）.

Chlorella Resistance to Dehydration Conditions and Protection Effect of Different Cryoprotecants on Chlorella Cells

[Objective] The aim was to study on the chlorella resistance to dehydration conditions and effect of different cryoprotecants on drying of chlorella cells.[Method]The protection effect of seven kinds of cryoprotecants on chlorella cells was analyzed.[Result]The protection effect of trehalose on chlorella cells was the best,and the survival rate of chlorella cells had improved by 50% with the applied trehalose concentration of 5%.[Conclusion]The result in this study had important guidance effect on the mutation breeding of chlorella.

Dehydration of bio-ethanol to ethylene over iron exchanged HZSM-5

Iron exchanged ZSM-5 with Si/Al ratio from 25 to 300 prepared by three consecutive ion exchanges was used for the dehydration of ethanol to ethylene.The iron exchanged ZSM-5（Si/Al=25） catalyst with an iron content of 0.46 wt%gave 97%-99%yield of ethylene at 98%-99%conversion of ethanol at 260℃ and 0.81 h^-1 liquid hourly space velocity.The high performance was maintained for60 d on-stream.X-ray diffraction,Fourier transform infrared spectroscopy of pyridine adsorption,NH3 temperature-programmed desorption and diffuse reflectance UV-vis spectroscopy were used for catalyst characterization.Ion exchange with iron decreased the total acidity of the zeolite,especially the strong acid sites and Bronsted acid sites.The doped iron species were distributed over Fe-ZSM-5 as predominantly isolated Fe^3＋.Therefore,the catalytic performance for ethanol dehydration to ethylene was improved.

Alkali-metal-modified ZSM-5 zeolites for improvement of catalytic dehydration of lactic acid to acrylic acid

Various ZSM-5 zeolites modified with alkali metals （Li, Na, K, Rb, and Cs） were prepared using ion exchange. The catalysts were used to enhance the catalytic dehydration of lactic acid （LA） to acrylic acid （AA）. The effects of cationic species on the structures and surface acid-base distributions of the ZSM-5 zeolites were investigated. The important factors that affect the catalytic performance were also identified. The modified ZSM-5 catalysts were characterized using X-ray diffraction, tempera- ture-programmed desorptions of NH3 and CO2, pyridine adsorption spectroscopy, and N2 adsorption to determine the crystal phase structures, surface acidities and basicities, nature of acid sites, specific surface areas, and pore volumes. The results show that the acid-base sites that are adjusted by alkali-metal species, particularly weak acid-base sites, are mainly responsible for the formation of AA. The KZSM-5 catalyst, in particular, significantly improved LA conversion and AA selectivity because of the synergistic effect of weak acid-base sites. The reaction was conducted at different reaction temperatures and liquid hourly space velocities （LHSVs） to understand the catalyst selectivity for AA and trends in byproduct formation. Approximately 98% LA conversion and 77% AA selectivity were achieved using the KZSM-5 catalyst under the optimum conditions （40 wt% LA aqueous solution, 365 ℃, and LHSV 2 h-1）.

Role of Bridge-bonded Formate in Formic Acid Dehydration to CO at Pt Electrode： Electrochemial in-situ Infrared Spectroscopic Study

Formic acid （HCOOH） decomposition at Pt film electrode has been studied by electrochem- ical in situ FTIR spectroscopy under attenuated-total-reflection configuration, in order to clarify whether bridge-bonded formate （HCOOD） is the reactive intermediate for COad for-mation from HCOOH molecules. When switching from HCOOH-free solution to HCOOH- containing solution at constant potential （E=0.4 V vs. RHE）, we found that immediately upon solution switch COad formation rate is the highest, while surface coverage of formate is zero, then after COad formation rate decreases, while formate coverage reaches a steady state coverage quickly within ca. 1 s. Potential step experiment from E=0.75 V to 0.35 V, reveals that formate band intensity drops immediately right after the potential step, while the COad signal develops slowly with time. Both facts indicate that formate is not the reactive intermediate for formic acid dehydration to CO.