Protein transduction domain of membrane penetrating peptide can efficiently deliver DNA and protein into mouse liver for gene therapy

BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of this study was to test a nonviral gene transferring vector's function of delivering DNA into liver cells to provide an important clue for gene transfer in liver gene therapy. METHODS: The complex of DNA and DNA delivering protein was injected into mice through their tail veins. Then the mice were killed and their liver tissue was sec- tioned. The gene transferring results were detected using a confocal laser scanning microscope. RESULTS: Fluorescence analysis indicated that both DNA- membrane penetrating peptide (MPP) complex and DNA- hepatocyte specific receptor binding domain ( HSRBD) - MPP complex could go into liver cells. The fluorescence value of liver cells in the DNA-HSRBD-MPP group was higher than that in the DNA-MPP group. CONCLUSIONS; MPP can successfully deliver DNA and protein into cells, and MPP with a HSRBD can specifically deliver DNA into liver cells. These have laid a foundation for further study on the nonviral liver cell gene delivering system.

Deliverability of wells in carbonate gas condensate reservoirs and the capillary number effect

With the development of the Tazhong No. 1 carbonate gas condensate reservoir in China, it has become more and more important to study the characteristics of gas condensate well deliverability. A single-well radial simulator for dual-permeability reservoirs was established to study the influences of fluid properties, permeability, and pressure drawdown on well deliverability with and without the capillary number effect. The simulation shows that well deliverability basically maintains its initial value and is not affected by the capillary number when the formation pressure is higher than dew-point pressure. However, well deliverability drops rapidly when the formation pressure is lower than dew-point pressure. Even if the condensate dropout is very low, well deliverability without the capillary number effect reduces to 50 percent of its initial value when reservoir pressure declines to 95 percent of dew-point pressure, but well deliverability is significantly improved if the capillary number effect exists. The capillary number effect is most significant when reservoir pressure is just lower than dew point pressure, then the effect decreases; the reduction of well deliverability is mainly caused by the reduction of gas relative permeability of the matrix system near the wellbore.

Tunable and Switchable Narrow Bandwidth Semiconductor-Saturable Absorber Mirror Mode-Locked Yb-Doped Fiber Laser Delivering Different Pulse Widths

The wavelength-tunable and switchable narrow bandwidth mode-locking operation is demonstrated in an all fiber laser based on semiconductor-saturable absorber mirror （SESAM）. Two narrow-band fiber Bragg gratings centered at 1029.9nm and 1032nm respectively with a polarization controller inserted between them are used to realize the wavelength switchable between 1029.9nm and 1032nm. The laser delivers different pulse widths of 7.5ps for 1030nm and 20ps for 1032nm. The maximum output power for both could reach -6.5mW at single pulse operation. The output wavelength couM be tuned to about 0.gnm intervals ranging from 1030.2nm to 1031.1 nm and from 1032.15nm to 1033.7nm with the temperature change of the fiber Bragg grating, respectively.

The establishment of a new deliverability equation considering threshold pressure gradient

The flowing mechanism of a low permeability gas reservoir is different from a conventional gas reservoir,especially for that with higher irreducible water saturation the threshold pressure gradient exists. At present,in all the deliverability equation,the additional pressure drop caused by the threshold pressure gradient is viewed as constant,but this method has big error in the practical application. Based on the non-Darcy steady flow equation,the limited integral of the additional pressure drop is solved in this paper and it is realized that the additional pressure drop is not a constant but has something to do with production data,and a new deliverability equation is derived,with the relevant processing method for modified isochronal test data. The new deliverability equation turns out to be practical through onsite application.

Modular Hydrogels for Drug Delivery

The development of novel drug delivery systems is an essential step toward controlled site-specific administration of therapeutics within the body. It is desirable for delivery vehicles to be introduced into the body through minimally invasive means and, these vehicles should be capable of releasing drug to their intended location at a controlled rate. Furthermore, it is desirable to develop drug delivery systems that are capable of in vivo to suffer degradation and to deliver the drug completely, avoiding the need to surgically remove the vehicle at the end of its useful lifetime. Hydrogels are of particular interest for drug delivery applications due to their ability to address these needs in addition to their good biocompatibility, tunable network structure to control the diffusion of drugs and, tunable affinity for drugs. However, hydrogels are also limited for drug delivery applications due to the often quick elution of drug from their highly swollen polymer matrices as well as the difficulty inherent in the injection of macroscopic hydrogels into the body. This paper presents an overview to the advances in hydrogels based drug delivery. Different types of hydrogels can be used for drug delivery to specific sites in the gastrointestinal tract ranging from the oral cavity to the colon. These novel systems exhibit a range of several peculiar properties which make them attractive as controlled drug release formulations. Moreover, such materials are biocompatible and can be formulated to give controlled, pulsed, and triggered drug release profiles in a variety of tissues.

SIMULATION OF A COLD ROLLING PLANT'S GOODS DELIVERY FORECASTING SYSTEM

The goods delivery process of one cold rolling plant was analyzed and the result shows it is necessary to reengineer and optimize this process. The goods delivery forecasting system was proposed after choosing suitable sampling methods. It is necessary to verify the efficiency of this optimization before the real system adjustment, which is costly and dangerous to some distance. A Simulation method, which employs object oriented paradigm to construct simulation system and stochastic approach to calculate process parameters, was put forward. The simulation result shows the feasibility of this method.

Nanoparticles as a tool to deliver drugs to the retina and brain:an update

Over the past few years,different neurontargeted nanoparticles(NPs)were designed to deliver drugs to enhance neuron protection and recovery,and much progress was made in our understanding of the uptake mechanism and the related physicochemical properties.Physicochemical properties attracting much attentions in NP's design and modification include particle size,surface hydrophobicity。

Delivering bad news to patients——the necessary evil

Delivering bad news regarding patient's health is an unavoidable task for physicians and medical students in their careers.This is especially difficult for beginners.Disclosing undesired medical information to patients is technically and mentally demanding.Only with proper communication skills and sufficient patient-contact practice can medical practitioners smoothly cope with this stressful mission.There are a number of common mistakes we fall into when delivering bad news,which might induce or exacerbate patients' anxiety.Sometimes we are prone to disclose medical information in a "straight-to-the-point" manner.This is often a futile attempt made on the physician's part to ease his or her own uncomfortableness when delivering bad news,but unfortunately it does no good to facilitate patients' acceptance of the actual condition.Instead of using medical jargons,physicians are obliged to use "layman's terms" that make better sense to their patients.Declaring a clinical diagnosis without corresponding explanation on the prognosis and the discussion of treatment can also bring much confusion and anxiety to patients.Another even worse situation might be that we finish the "lecture" in our own words without confirming the patient's comprehension and addressing their questions.Professionals have recommended a six-step protocol on delivering bad news to patients:"SPIKES":S-Setting up the interview;P-assessing the patient's Perception;I-obtaining the patient's Invitation;K-giving Knowledge and information to the patient;E-addressing the patient's Emotions with Empathic responses;S-Strategy and Summary.

Parcels Delivered in 2019 to Reach 60 Billion

The State Post Bureau(SPB)predicts that the volume of parcels delivered this year will reach 60 billion,a 20 percent year-onyear growth.

Research on the Value Positioning of University Library Based on Customer Delivered Value

With the rise of various new reading media and the rapid development of the digitization of paper resources,the traditional function positioning of university libraries based on information query is facing unprecedented challenges.How to deal with the change of social information?The library should be demand-oriented,re-examine its own value and find a new starting point.With the help of the concept of customer delivered value and based on 4P theory,this paper constructs the value chain of university library from the dimensions of product,image,personnel and service,and forms a multi-dimensional development positioning system.

TRANSFORM THE MECHANISM OF RENMINBI’S EXCHANGE RATE SYSTEM IN CONFORMITY WITH THE DEMAND OF MARKET ECONOMY——The academic report delivered at the Annual Meeting of NNCIFS on October 1993

Exchange rate functions under systems of centrallyplanned economy and market economy are quite different,and the mechanism to determine such exchange rate is alsodifferent. To adopt a socialist market economic system inChina, the exchange rate mechanism of Renminbi must bethoroughly transformed. To accomplish this goal, I believethere are three problems that must be solved: first,

ZTE Teams with Polkomtel to Deliver Unprecedented Access to Multimedia Applications

ZTE Corporation,a leading global provider of telecommunications equipment and network solutions,announced on June 6,2007 that it has signed a strategic cooperation agreement with Polkomtel S.A.,one of the top three mobile telephony operators in Poland.

ZTE Delivers CDMA WLL Equipment and Full Services to Telecom Egypt

At ITU Telecom Africa 2004,Telecom Egyptofficially announced that it had selected ZTECorporation,the leading telecom solutionsprovider in the world,to be its global strategicpartner,and that ZTE would undertake theconstruction of its CDMA WLL network anddeliver full services to Telecom Egypt.Akil Beshir,Chairman of Telecom

Treatment of Unicameral and Aneurysmal Bone Cysts by Minimally Invasive Percutaneous Injection of Grafton DBF Putty Using the Kyphon Cement Delivery System

Background: Simple Unicameral and Aneurysmal Bone Cysts are benign lesions that may heal spontaneously especially after fracture which may be the first symptom. However, often size increases causing pain, and complications of fractures can severely compromise the patient. Aim: The results in a series of cases treated minimally invasive using a new device for the application of allogenic bone material appear highly promising and shall be presented. Patients and Methods: Eight consecutive patients with symptomatic Unicameral Bone Cysts (UBC) were treated by percutaneous instillation of Grafton? DBF Putty (demineralised allogenic bone containing fibers) mixed with autologous bone marrow using the Kyphon? Cement Delivery System (Medtronic), which allows the injection of this high viscosity paste by controlled high pressure. Five patients with Aneurysmal Bone Cysts (ABC) were treated accordingly after inactivation by Aethoxysclerol 3% and lacking bone formation. Using this approach a high rate of bone regeneration was observed in these patients at 8 months to 5 years follow-up (f/u). Conclusion: The presented technique of a minimally invasive biologic treatment led to highly satisfying results using the Grafton? DBF Putty with its higher potential for bone regeneration than demineralized bone matrix not containing fibres (DBM).

FY-4 Satellite Delivered to Customer

On September 25,the on-orbit delivery ceremony for China’s new generation geostationary meteorological satellite FY-4 was held in Beijing.China Aerospace Science and Technology Corporation（CASC）,the manufacturer of the satellite officially delivered FY-4 to the customer,the China Meteorological Administration（CMA）for operation.

FY-2G Passed In-orbit Commissioning Review and Delivered to Customer

The manufacturer of the FY-2G meteorological satellite delivered the satellite to its customer CMA at an in-orbit delivery ceremony held on April 29 in Beijing.The FY-2G meteorological satellite developed by the Shanghai Academy of Spaceflight Technology(SAST),is the second satellite in the third batch of FY-2geostationary meteorological satellite program and was launched on Decem-

Regional supply chain delivering force into textile foreign trade

Textiles and apparel might be a global industry,but the latest statistics show trade patterns remain largely regional.However,changes are happening,and align closely with the shifting sourcing strategies of fashion brands and retailers and related free trade agreements-as shown in this new analysis by Dr Sheng Lu,associate professor in Fashion and Apparel Studies at the University of Delaware.

Address Delivered to the Christian Leaders in the West Of the United States

This is my second visit to Los Angeles as the Director of the State Administration for Religious Affairs to exchange views with all the American Christian friends present. I believe what you want to learn best is the real situation of Chinese Christianity at present. I can tell you with pleasure that Chinese Christians are running their church independently and has thus removed the "cap" of "foreigness". It has become a religion that eulogizes love and traditional culture. As a reli-