Currently hemodynamic-based functional MRI technique is of limitation in temporal resolution. As neural activities in the brain accompany with current induced neuronal magnetic fields （NMF）, it is possible to utiliz...Currently hemodynamic-based functional MRI technique is of limitation in temporal resolution. As neural activities in the brain accompany with current induced neuronal magnetic fields （NMF）, it is possible to utilize MRI to detect NMF directly thus to improve the temporal resolution. In this work, the contribution of dendrite branch to NMF is investigated by numeric simulation. The results indicate that the existence of dendrite branch may enhance the detectability of NMF by MRI directly.展开更多
The histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit(EZH2)-mediated trimethylation of histone H3 lysine 27(H3K27me3)regulates neural stem cell proliferation and fate specificity thro...The histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit(EZH2)-mediated trimethylation of histone H3 lysine 27(H3K27me3)regulates neural stem cell proliferation and fate specificity through silencing different gene sets in the central nervous system.Here,we explored the function of EZH2 in early post-mitotic neurons by generating a neuron-specific Ezh2 conditional knockout mouse line.The results showed that a lack of neuronal EZH2 led to delayed neuronal migration,more complex dendritic arborization,and increased dendritic spine density.Transcriptome analysis revealed that neuronal EZH2-regulated genes are related to neuronal morphogenesis.In particular,the gene encoding p21-activated kinase 3(Pak3)was identified as a target gene suppressed by EZH2 and H3K27me3,and expression of the dominant negative Pak3 reversed Ezh2 knockout-induced higher dendritic spine density.Finally,the lack of neuronal EZH2 resulted in impaired memory behaviors in adult mice.Our results demonstrated that neuronal EZH2 acts to control multiple steps of neuronal morphogenesis during development,and has long-lasting effects on cognitive function in adult mice.展开更多
基金supported by the National Natural Science Foundation of China under Grant No. 60571019, 30525030, 30870655, and 60736029.
文摘Currently hemodynamic-based functional MRI technique is of limitation in temporal resolution. As neural activities in the brain accompany with current induced neuronal magnetic fields （NMF）, it is possible to utilize MRI to detect NMF directly thus to improve the temporal resolution. In this work, the contribution of dendrite branch to NMF is investigated by numeric simulation. The results indicate that the existence of dendrite branch may enhance the detectability of NMF by MRI directly.
基金The study was supported by Grants from the NIH(R01NS064288,R01NS085176,R01GM111514,and R01EY027347)the Craig H.Neilsen Foundation(259450)the BrightFocus Foundation(G2017037).
文摘The histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit(EZH2)-mediated trimethylation of histone H3 lysine 27(H3K27me3)regulates neural stem cell proliferation and fate specificity through silencing different gene sets in the central nervous system.Here,we explored the function of EZH2 in early post-mitotic neurons by generating a neuron-specific Ezh2 conditional knockout mouse line.The results showed that a lack of neuronal EZH2 led to delayed neuronal migration,more complex dendritic arborization,and increased dendritic spine density.Transcriptome analysis revealed that neuronal EZH2-regulated genes are related to neuronal morphogenesis.In particular,the gene encoding p21-activated kinase 3(Pak3)was identified as a target gene suppressed by EZH2 and H3K27me3,and expression of the dominant negative Pak3 reversed Ezh2 knockout-induced higher dendritic spine density.Finally,the lack of neuronal EZH2 resulted in impaired memory behaviors in adult mice.Our results demonstrated that neuronal EZH2 acts to control multiple steps of neuronal morphogenesis during development,and has long-lasting effects on cognitive function in adult mice.
