In sensorimotor cortical slices of guinea pig using local iontophoretic application of glutamate to the soma and dendrites it was found that a decrease of temperature of incubating fluid from 34 to 21°C - 22°...In sensorimotor cortical slices of guinea pig using local iontophoretic application of glutamate to the soma and dendrites it was found that a decrease of temperature of incubating fluid from 34 to 21°C - 22°C changes the somatic responses to the local injection of glutamate to the dendritic loci, while the responses to iontophoretic application of glutamate to the soma remain unchanged. Hypothermic changes in reactivity to dendritic stimulation start below 30°C and coincide with changes in the spontaneous activity of neurons, both in the direction of increasing and decreasing the frequency of firing in different nerve cells. On hypothermic decrease of spontaneous activity, the latencies of evoked dendritic responses on the soma became more longer, while on hypothermic increase of firing level, somatic spike responses to iontophoretic application of glutamate to dendritic loci appeared with shorter latencies. Hypothermic changes in the physiological parameters of neurons were accompanied by a drop in spike amplitude at the same temperature and with its further decrease. At the same time, there was a decrease of spike reaction to iontophoretic application of acetylcholine below 30°. It is proposed that the reason for hypothermic changes of neuronal activity is decreasing rate of M-cholinergic process at 27°C - 29°C which leads to opening K<sup>+</sup> channels of neuronal membranes and hence to attenuation of conductive function of dendrites and to imbalance of K<sup>+</sup> ion homeostasis. Peculiarities of hypothermic regulation of neuronal spike activity depend on individual functional properties of cortical neurons.展开更多
Cluster analysis is a numerical taxonomy to specimens according to the measurement data of the multivariate of the specimens by mathematical method. At present in geological researches more and more geologists discard...Cluster analysis is a numerical taxonomy to specimens according to the measurement data of the multivariate of the specimens by mathematical method. At present in geological researches more and more geologists discard the qualitative taxonomy established by traditional geology and, by means of cluster analysis establish the quanti-展开更多
The introduction of Golgi impregnation method in 1873 marked the beginning of modern neuroanatomy. The central dendro-architecture revealed by Golgi method is one of the cornerstones of contemporary neuroscience. Usin...The introduction of Golgi impregnation method in 1873 marked the beginning of modern neuroanatomy. The central dendro-architecture revealed by Golgi method is one of the cornerstones of contemporary neuroscience. Using cholera toxin conjugated HRP (CT-HRP) or choleragenoid conjugated HRP (CB-HRP) as retrogradely labeling marker, we have discovered several types of dendro-architecture which cannot be revealed by Golgi method or wheat germ agglutinin conjugated HRP (WG-HRP). We call these dendrites which are refractory to Golgi展开更多
Ⅰ. GENERAL CONDITION Situated on the northern slope of Daxue Mountain (大雪山) in the western part of Qilian Mts., Glacier No. 12 of Laohugou (老虎沟) is the longest valley glacier, covering an area of 21.45 km^2 wit...Ⅰ. GENERAL CONDITION Situated on the northern slope of Daxue Mountain (大雪山) in the western part of Qilian Mts., Glacier No. 12 of Laohugou (老虎沟) is the longest valley glacier, covering an area of 21.45 km^2 with 10 km in length. Its physical property is of significance in the continental glaciers of China. In 1959—62, the first Glacier Location Observa-展开更多
Injections of cholera toxin-HRP (CTHRP), wheat germ agglutinin-HRP (WGHRP) or free HRP (FHRP) were made in the tongues of 22 adult rats whose hypoglossal nerves were left bilaterally intact or unilaterally severed. Af...Injections of cholera toxin-HRP (CTHRP), wheat germ agglutinin-HRP (WGHRP) or free HRP (FHRP) were made in the tongues of 22 adult rats whose hypoglossal nerves were left bilaterally intact or unilaterally severed. After 24 hour’s survival, intracordial perfusions were carried out to fix the brians according to procedure Ⅱ of Rosen and Mesulam. Cornal sections of medulla at 50 μm on cryostate microtome were undertaken by TMB method of Mesulam At the dosage of 5—10 μg HRP in 1—30 μl phosphate buffer, CTHRP and WGHRP constantly revealed perikarya, axons and many展开更多
文摘In sensorimotor cortical slices of guinea pig using local iontophoretic application of glutamate to the soma and dendrites it was found that a decrease of temperature of incubating fluid from 34 to 21°C - 22°C changes the somatic responses to the local injection of glutamate to the dendritic loci, while the responses to iontophoretic application of glutamate to the soma remain unchanged. Hypothermic changes in reactivity to dendritic stimulation start below 30°C and coincide with changes in the spontaneous activity of neurons, both in the direction of increasing and decreasing the frequency of firing in different nerve cells. On hypothermic decrease of spontaneous activity, the latencies of evoked dendritic responses on the soma became more longer, while on hypothermic increase of firing level, somatic spike responses to iontophoretic application of glutamate to dendritic loci appeared with shorter latencies. Hypothermic changes in the physiological parameters of neurons were accompanied by a drop in spike amplitude at the same temperature and with its further decrease. At the same time, there was a decrease of spike reaction to iontophoretic application of acetylcholine below 30°. It is proposed that the reason for hypothermic changes of neuronal activity is decreasing rate of M-cholinergic process at 27°C - 29°C which leads to opening K<sup>+</sup> channels of neuronal membranes and hence to attenuation of conductive function of dendrites and to imbalance of K<sup>+</sup> ion homeostasis. Peculiarities of hypothermic regulation of neuronal spike activity depend on individual functional properties of cortical neurons.
文摘Cluster analysis is a numerical taxonomy to specimens according to the measurement data of the multivariate of the specimens by mathematical method. At present in geological researches more and more geologists discard the qualitative taxonomy established by traditional geology and, by means of cluster analysis establish the quanti-
文摘The introduction of Golgi impregnation method in 1873 marked the beginning of modern neuroanatomy. The central dendro-architecture revealed by Golgi method is one of the cornerstones of contemporary neuroscience. Using cholera toxin conjugated HRP (CT-HRP) or choleragenoid conjugated HRP (CB-HRP) as retrogradely labeling marker, we have discovered several types of dendro-architecture which cannot be revealed by Golgi method or wheat germ agglutinin conjugated HRP (WG-HRP). We call these dendrites which are refractory to Golgi
文摘Ⅰ. GENERAL CONDITION Situated on the northern slope of Daxue Mountain (大雪山) in the western part of Qilian Mts., Glacier No. 12 of Laohugou (老虎沟) is the longest valley glacier, covering an area of 21.45 km^2 with 10 km in length. Its physical property is of significance in the continental glaciers of China. In 1959—62, the first Glacier Location Observa-
文摘Injections of cholera toxin-HRP (CTHRP), wheat germ agglutinin-HRP (WGHRP) or free HRP (FHRP) were made in the tongues of 22 adult rats whose hypoglossal nerves were left bilaterally intact or unilaterally severed. After 24 hour’s survival, intracordial perfusions were carried out to fix the brians according to procedure Ⅱ of Rosen and Mesulam. Cornal sections of medulla at 50 μm on cryostate microtome were undertaken by TMB method of Mesulam At the dosage of 5—10 μg HRP in 1—30 μl phosphate buffer, CTHRP and WGHRP constantly revealed perikarya, axons and many
