Study on Mechanism of Dendrobium officinale Kimura et Migo in Preventing and Treating Exercise-induced Muscle Damage(EIMD)in Rats

[Objectives]This study was conducted to observe the mechanism of Dendrobium officinale Kimura et Migo on gastrocnemius muscle in rats with exercise-induced muscle damage(EIMD).[Methods]The micro-injury model of skeletal muscle was established by treadmill training.Forty two SD rats were randomly divided into a control group,1,12 and 24 h exercise groups,D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group,and D.officinale 2 ml+24 h exercise group,with 6 rats in each group.Various D.officinale groups were given the drug once in the morning and once in the evening at a dose of 2 ml/time,a week in advance.Except for the quiet group,the samples were collected from the 1,12 and 24 h exercise groups after anesthesia following 1,12 and 24 h of exercise for the last time,respectively,and the D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group and D.officinale 2 ml+24 h exercise group were also sampled after anesthesia following 1,12 and 24 h of exercise for the last time,respectively.The contents of ATP,CK-MM and CK in rat serum were determined by enzyme-linked immunosorbent assay(ELISA).The histopathological changes of gastrocnemius muscle were observed by HE staining.PCR and Western-blot detection were carried out to analyze the effects of D.officinale on IGF-1 mRNA and protein expression in gastrocnemius muscle.[Results]Compared with the quiet group,the ATP contents in the serum of rats in the 1,12 and 24 h exercise groups significantly decreased(P<0.01),while the CK and CK-MM contents significantly increased(P<0.01).The expression of IGF-1 mRNA and protein in the gastrocnemius muscle tissue significantly increased(P<0.01).Compared with the 1 h exercise group,the ATP content and IGF-1 protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+1 h exercise group significantly increased(P<0.05),while the CK and CK-MM contents significantly decreased(P<0.01).Compared with the 12 h exercise group,the D.officinale liquid+12 h exercise group showed a significant increase in ATP content(P<0.01),significant increases in IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue(P<0.01),and significant decreases in CK and CK-MM contents(P<0.01).Compared with the 24 h exercise group,the ATP content and IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+24 h exercise group significantly increased(P<0.01),while the CK and CK-MM contents significantly decreased(P<0.01).From the pathological tissue morphology of the gastrocnemius muscle in rats with EIMD treated with D.officinale,it could be concluded that the gastrocnemius muscle of each exercise group was significantly damaged,and the damage was significantly alleviated after administration of D.officinale liquid.[Conclusions]The effects and mechanism of D.officinale on prevention and treatment of EIMD in rats might be related to the promotion of IGF-1 mRNA and protein expression in injured tissues by reducing ATP energy consumption,CK-MM and CK activity.

Functions of Plant Growth Substances in the Growth of Dendrobium officinale Kimura et Migo

[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substance on the growth and development of D. officinale Kimura et Migo. [Results] Plant growth regulators play an important role in the process of tissue culture rapid propagation of D. officinale Kimura et Migo,and the main regulatory pathways are as follows.( i) Plant growth regulators promote the growth and development of D. officinale Kimura et Migo through regulating the changes of its endogenous hormones.( ii) Plant growth regulators affect the accumulation of polysaccharide content in D. officinale Kimura et Migo through regulating the changes in chlorophyll content and enzymes involved in sucrose metabolism.( iii) Plant growth regulators can increase the activity of antioxidant enzymes of D. officinale Kimura et Migo,to increase its stress resistance. [Conclusions]Using molecular biology techniques,combined with the changes of plant hormones in D. officinale Kimura et Migo,and analysis on key enzyme in the synthesis of plant hormones and gene expression,it is feasible to study the rules o changes in the content of active content of D. officinale Kimura et Migo.

Efficacy Evaluation of 16% Validamycin·thifluzamide SC against Selerotium rolfsii in Dendrobium officinale Kimura et Migo

[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.

Research Progress of Dendrobium officinale Kimura et Migo

The effects of light on the quality and related genes of Dendrobium officinale were summarized, and the main chemical components and pharmacological effects of D. officinale were analyzed. The biomass accumulation of D. officinale is related to light quality and light intensity. Monochromatic light quality can make polysaccharide accumulate, and mixed light quality can promote cell proliferation and component accumulation of D. officinale. Under different light intensity, the polysaccharide content of D. officinale is different, and it changes with the change of light intensity. When a certain intensity is exceeded, the polysaccharide content decreases with the increase of light intensity. The chemical components of D. officinale are polysaccharides, alkaloids, amino acids, volatile components, etc. The analysis of the growth and components of D. officinale provides reference and basis for improving the quality and chemical components of D. officinale and promotes the research and development of related products of D. officinale.

New Utilization Model of Karst Rocky Desertified Land in Southwest China:Ecological Planting of Dendrobium officinale Kimura et Migo on Bare Rocky Mountains

The Yunnan-Guangxi-Guizhou rocky desertified area in southwestern China is one of the contiguous extremely poor areas identified in the Outline of Poverty Alleviation and Development in the Rural Areas of China(2011-2020).In rocky desertified areas,due to long-term severe soil erosion,large areas of bedrock are exposed or gravels are accumulated.This bare rock and gravel-mulched land has become the main land type in the rocky mountains.Under normal circumstances,it cannot be directly used for agriculture,forestry,animal husbandry,and fishery production,and is classified as land that is difficult to be utilized.In recent years,in Debao County,Baise City,Guangxi Zhuang Autonomous Region,as a deeply impoverished county in the Yunnan-Guangxi-Guizhou rocky desertified area,a new land use model of karst rocky desertified land,that is,planting Dendrobium officinale Kimura et Migo on bare rocky ground,has emerged,and certain poverty alleviation benefits have been achieved initially.In this article,on the basis of analyzing the suitability of planting D.officinale Kimura et Migo on karst rocky desertified land,the practice of planning D.officinale Kimura et Migo on the rocky desertified land in Debao County was elucidated,and then suggestions for reasonable promotion of ecological planting of D.officinale Kimura et Migo in karst rocky desertified areas were put forward.

NaCl浓度对铁皮石斛次生代谢物的影响

为探索不同浓度的盐胁迫对铁皮石斛(Dendrobium officinale Kimura et Migo)次生代谢物含量的影响,设置0、50、100、150、200 mmol/L NaCl对铁皮石斛进行盐胁迫处理。结果表明,不同浓度的盐胁迫对铁皮石斛体内次生代谢物含量的影响有所差异,50~100 mmol/L NaCl处理可使铁皮石斛体内类黄酮、氨基酸和总酚等次生代谢物含量明显增加,50 mmol/L NaCl处理可使铁皮石斛体内总多糖含量明显增加,活性成分有效提高,中药材价值极大增强。

铁皮石斛组织培养研究

以铁皮石斛(Dendrobium officinale Kimura et Migo)幼嫩带芽茎段作为试验材料,利用植物组织培养技术研究生长调节剂配比、天然添加物香蕉泥对铁皮石斛腋芽的诱导、增殖的影响;将诱导出的组培苗移栽到不同栽培基质中进行移栽驯化。结果表明,最适茎段腋芽诱导的培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA+香蕉泥100 g/L;适合芽增殖的培养基为MS+2.0 mg/L 6-BA+0.5 mg/L NAA+香蕉泥100 g/L;铁皮石斛组培苗最适移栽单一基质为锯末,复合基质为松树皮与锯末,体积比为7∶3。

多效唑(PP333)对铁皮石斛瓶内开花的促进作用

为了研究不同浓度多效唑PP333和不同预处理时间对铁皮石斛瓶内开花的影响。本研究以无菌铁皮石斛(Dendrobium officinale Kimura et Migo)原球茎为试验材料,在培养基中添加不同浓度的多效唑,并设定不同的预培养时间。结果显示,对照处理(未添加多效唑)的铁皮石斛组培苗未出现开花现象,而在培养基中添加不同浓度的多效唑后,铁皮石斛组培苗开花率均显著提高。当多效唑浓度为1.0 mg/L、预培养时间为30 d时,铁皮石斛组培苗开花率达最大(62.50%),花期最长(59.33 d);当多效唑浓度为1.0 mg/L、预培养时间为50 d时,铁皮石斛组培苗始花期最早(21.67 d),花朵品质最好。因此,本研究获得了石斛瓶内开花的最适PP333处理浓度和最佳预培养时间,为铁皮石斛试管花卉的开发提供参考,同时为其他兰科植物的瓶内开花提供理论和技术指导。