基于稀疏重建的EMD脉搏色素密度谱的处理

基于脉搏血氧计的吲哚青绿(Indocyanine green,ICG)脉搏密度谱测量方法是无创肝储备功能的主要检测原理。近红外脉搏色素密度谱的提取及处理直接影响着肝储备功能检测参数的计算精度。针对自行研发的脉搏色素密度谱采集装置采集的近红外光谱信号的光谱噪声及干扰问题,采用了结合稀疏二进制重建的经验模态分解法对ICG密度谱进行处理。将处理后提取的ICG密度谱进行分析计算,得到的ICG血浆消失率值与报告值相比较,其最大相对误差为5.1%;而平均相对误差为3.2%。实验结果表明,结合自适应稀疏二进制重构的经验模态分解方法对消除色素光谱的噪声是有效的,有效地提高了脉搏色素密度谱的测量精度,从而提高肝储备功能检测参数的精度。为近红外脉搏色素光谱提取优化提供了一种新方法,为抗干扰的无创肝储备功能的检测提供理论依据和实用方法。

对肝储备功能分析系统参考数值的补充和评价

目的对新一代定量快速肝储备功能分析系统（DDG）的指标数值进行修订和补充，并加以评价。方法我科从2005年5月至2009年9月检测1228例，剔除肝病等患者，对符合规定的982例次DDG结果进行统计分析，并应用于临床实践。结果各统计指标和既往参考值相比，范围略有扩大；用于临床后，术后肝脏功能失代偿的发生率由34．4％降至23．5％。结论本数值结果作为肝脏储备功能的评估，临床上可减少有关肝脏术后的并发症。

Partial characterization of superoxide dismutase activity in the Barber pole worm-Haemonchus contortus infecting Capra hircus and abomasal tissue extracts

Objective:To determine the activity of superoxide dismutase(SOD) in the male and female haematophagous caprine worms,Haemonchus contortus infecting Capra hircus,and their E/S products and also to analyse the effect of Haemonchus infection on the level of host SOD.Methods:The SOD activity was analysed by using the pyrogallol autoxidation assay and non-denaturing polyacrylamide gel electrophoresis followed by specific enzyme staining by riboflavin-nitroblue tetrazolium method.Results:The adult females were found to have higher enzyme activity than the male worms.Appreciable amount of SOD activity was also detected in the worm culture medium and female worms secreted more SOD in comparison to the male parasites.The SOD activity was negatively correlated to the worm burden.Statistically significant decrease in SOD activity(P<0.05) was observed in the heavily infected host tissue in comparison to the control non-infected host tissue.SOD profile of the crude extracts of both the sexes revealed polymorphism and a fast migrating activity band being characteristic of E/S products.The SOD activities were found highly sensitive to potassium cyanide indicating the Cu/Zn form of SOD.Conclusions:Haemonchus contortus is a key model parasite for drug and vaccine discovery.The presences of SOD activity in appreciable amount in the parasite as well as its E/S products indicate that it has a well-developed active antioxidant system to protect itself from the host immune attack.SOD could be the target for vaccine development which is the need of the hour as mass drug administration for parasite control has resulted in anthelmintic resistance across the globe and threatens the viability of sheep and goat industry in many regions of the world.The infection with Haemonchus causes a drastic reduction in SOD activity of the host tissue thus effecting its protective potential.One characteristic SOD band was found in the females which was not present in any other preparations and thus could be exploited for further studies on diagnostic/control measures.

350m氦氧模拟饱和潜水过程中潜水员坐位踏车时心缩间期的变化

在350m氦氧模拟饱和潜水过程中,对4名男性潜水员采用耳密度图导数图方法观察坐位踏车时心缩间期变化。在压力(300、230、135m)下和减压后的主要变化是等容收缩期、射血前期(PEP)和PEP/左室射血时间加大,与加压前比较有显著差异,尤其在踏车负荷加重时更为明显。提示心肌收缩力受高气压的影响而降低。

工期高血压病50例坐位踏车心缩间期变化观察

本文对50例男性Ⅰ期高血压病患者,采用耳密度图导数图、心电图和心音图三项同步描记的方法进行坐位踏车负荷中的心缩间期变化观察。主要的发现是PEP、ICT和PEP/LVET比值较正常对照组大,且随负荷量的加重而更显著,尤其PEP/LVET的最低比值(称“谷底”值)出现较早。提示此类患者在重负荷时心肌收缩力减低,心脏贮备相对较少。