The aim of this study was to evaluate the adhesion of human fetal osteoblast cells (CRL-11372) in vitro at 24 h on commercially pure titanium (cp Ti) metal surfaces’ crystalline structure and surface roughnesses that...The aim of this study was to evaluate the adhesion of human fetal osteoblast cells (CRL-11372) in vitro at 24 h on commercially pure titanium (cp Ti) metal surfaces’ crystalline structure and surface roughnesses that are modified by polishing, sand blasting (with alumina (Al2O3)), sand blasting and coating (with titanium oxide (TiO2)), and sand blasting and etching (with oxalic acid). Modified surfaces were characterized quantitatively by a non-contacting optical profilometer in terms of their Rz and Ra values and surface profile diagrams were obtained. These surfaces were characterized qualitatively by scanning electron microscope (SEM) micrographs. The crystalline structures of the coatings were characterized by X-ray diffraction (XRD). CRL-11372 cells were cultured for 24 h and evaluated for their mean total cell counts. Cell morphologies were examined by SEM micrographs. Data were compared by Kruskal-Wallis test followed by Post Hoc LSD test comparisons. SEM micrographs showed variations among the topographies of the surfaces and the morphologies of the cells adhered to these four different surfaces. Cell adhesion was affected by neither Ti chemical composition nor surface roughness within the Ra and Rz parameters used.展开更多
In the present work, osteoblast behavior on a hierarchical micro-/nano-structured titanium surface was investigated. A hi- erarchical hybrid micro-/nano-structured titanium surface topography was produced via Electrol...In the present work, osteoblast behavior on a hierarchical micro-/nano-structured titanium surface was investigated. A hi- erarchical hybrid micro-/nano-structured titanium surface topography was produced via Electrolytic Etching （EE）. MG-63 cells were cultured on disks for 2 h to 7 days. The osteoblast response to the hierarchical hybrid micro-/nano-structured titanium surface was evaluated through the osteoblast cell morphology, attachment and proliferation. For comparison, MG-63 cells were also cultured on Sandblasted and Acid-etched （SEA） as well as Machined （M） surfaces respectively. The results show signifi- cant differences in the adhesion rates and proliferation levels of MG-63 cells on EE, SLA, and M surfaces. Both adhesion rate and proliferation level on EE surface are higher than those on SLA and M surfaces. Therefore, we may expect that, comparing with SLA and M surfaces, bone growth on EE surface could be accelerated and bone formation could be promoted at an early stage, which could be applied in the clinical practices for immediate and early-stage loadings.展开更多
基金Preparation and characterization of the commercially pure titanium metal surfaces in this study were supported by The Research Support Unit of Istanbul University as the project no 1749/21122001.
文摘The aim of this study was to evaluate the adhesion of human fetal osteoblast cells (CRL-11372) in vitro at 24 h on commercially pure titanium (cp Ti) metal surfaces’ crystalline structure and surface roughnesses that are modified by polishing, sand blasting (with alumina (Al2O3)), sand blasting and coating (with titanium oxide (TiO2)), and sand blasting and etching (with oxalic acid). Modified surfaces were characterized quantitatively by a non-contacting optical profilometer in terms of their Rz and Ra values and surface profile diagrams were obtained. These surfaces were characterized qualitatively by scanning electron microscope (SEM) micrographs. The crystalline structures of the coatings were characterized by X-ray diffraction (XRD). CRL-11372 cells were cultured for 24 h and evaluated for their mean total cell counts. Cell morphologies were examined by SEM micrographs. Data were compared by Kruskal-Wallis test followed by Post Hoc LSD test comparisons. SEM micrographs showed variations among the topographies of the surfaces and the morphologies of the cells adhered to these four different surfaces. Cell adhesion was affected by neither Ti chemical composition nor surface roughness within the Ra and Rz parameters used.
文摘In the present work, osteoblast behavior on a hierarchical micro-/nano-structured titanium surface was investigated. A hi- erarchical hybrid micro-/nano-structured titanium surface topography was produced via Electrolytic Etching （EE）. MG-63 cells were cultured on disks for 2 h to 7 days. The osteoblast response to the hierarchical hybrid micro-/nano-structured titanium surface was evaluated through the osteoblast cell morphology, attachment and proliferation. For comparison, MG-63 cells were also cultured on Sandblasted and Acid-etched （SEA） as well as Machined （M） surfaces respectively. The results show signifi- cant differences in the adhesion rates and proliferation levels of MG-63 cells on EE, SLA, and M surfaces. Both adhesion rate and proliferation level on EE surface are higher than those on SLA and M surfaces. Therefore, we may expect that, comparing with SLA and M surfaces, bone growth on EE surface could be accelerated and bone formation could be promoted at an early stage, which could be applied in the clinical practices for immediate and early-stage loadings.
