Introduction: Transitioning to more efficacious Antiretrovirals for HIV infected pregnant women and infant prophylaxis has reduced Mother to child transmission of HIV significantly. This study aimed to determine HIV i...Introduction: Transitioning to more efficacious Antiretrovirals for HIV infected pregnant women and infant prophylaxis has reduced Mother to child transmission of HIV significantly. This study aimed to determine HIV infection status in HIVexposed infants who had their first DNA polymerase chain reaction test in our molecular Laboratory. Subjects, Materials and Methods: Dried Blood Spots for HIV DNA results from 5 states between 2009 and 2020 were analyzed in the PCR laboratory of the Federal Teaching Hospital, Gombe. Results: Nine thousand eight hundred and twenty-three Human Immunodeficiency Virus Deoxyribonucleic acid polymerase Chain Reaction results were analysed;4937 (50.2%) were males. During the study period, there was an overall declining trend in the mother-to-child transmission rate from 3.8% in 2009 to 1.0% in 2020. 6120 (62.3%) of HIV + mothers received Highly active antiretroviral therapy HAART before pregnancy. 7845 (76.2%) of the infants received Nevirapine prophylaxis. Dried blood spot samples were collected from 4077 (41.5%) at 6 - 8 weeks. 8438 (85.9%) received cotrimoxazole. 9469 (96.4%) were ever breastfed. Of the 9823 HIV DNA PCR results, 255 (2.6%) were positive while 69/4077 (1.7%) and 109/2662 (4.1%) were positive for HIV DNA at 6 - 8 weeks and > 12 weeks respectively. (p = 0.001). 86/747 (11.5%) of infants whose HIV-positive mothers received no ARVS were HIV DNA positive. (p = 0.001). 106/884 (12.0%) of infants who had no Antiretroviral prophylaxis had positive HIV DNA results;7/413 (1.7%) with Zidovudine/Nevirapine prophylaxis had positive results. (p = 0.001). 246/9469 (2.6%) of infants that were ever breastfed were positive for HIV DNA;11/354 (3.0%) that never breastfed had positive HIV DNA. Conclusion: Lack of maternal/infant ARVs and prolonged breastfeeding increased the risk of infant HIV infection.展开更多
Theoretical analysis of the DNA dynamics reveals that interaction between the single solitons and plane wave implies Smale-horseshoe chaos in the double helices. Solutions of the chaotic solitons are derived from a di...Theoretical analysis of the DNA dynamics reveals that interaction between the single solitons and plane wave implies Smale-horseshoe chaos in the double helices. Solutions of the chaotic solitons are derived from a direct perturbation technique. It is demonstrated that to produce the bounded chaotic solitons, velocities of the solit ons nust be the same and equal to propagation velocity of the plane wave in DNA. The result shows that the DNA structure may be destroyed by the long action of an electromagnetic wave. It also supplies a useful method for controlling the velocities and unboundedness of the DNA motion in a tumour cell by using a plane wave.展开更多
目的:分析不同保存温度及溶血、脂血血液标本对乙型肝炎病毒(HBV)脱氧核糖核酸(DNA)检测结果的影响。方法:选取2021年1-12月甘肃省肿瘤医院检验科的84份脂血血液标本、127份溶血血液标本及46份性状正常的血液标本分别作为脂血组、溶血...目的:分析不同保存温度及溶血、脂血血液标本对乙型肝炎病毒(HBV)脱氧核糖核酸(DNA)检测结果的影响。方法:选取2021年1-12月甘肃省肿瘤医院检验科的84份脂血血液标本、127份溶血血液标本及46份性状正常的血液标本分别作为脂血组、溶血组及对照组。检测对照组不同保存温度不同保存时间HBV DNA Ct值。检测脂血组及对照组标本中的HBV DNA Ct值。检测溶血组及对照组所有血液标本HBV DNA Ct值。比较对照组不同保存温度下4 h、24 h、48 h、72 h及1周的HBV DNA Ct值。比较对照组及脂血组的HBV DNA Ct值及HBV DNA检出率。比较对照组及溶血组的HBV DNA Ct值及HBV DNA检出率。结果:25℃下保存1周HBV DNA Ct值高于25℃下保存4 h、24 h、48 h、72 h,差异有统计学意义(P<0.05);37℃下保存72 h、1周HBV DNA Ct值高于37℃下保存4 h、24 h、48 h,差异有统计学意义(P<0.05);37℃下保存48 h HBV DNA Ct值高于37℃下保存4 h、24 h,差异有统计学意义(P<0.05)。-30℃下保存48 h、72 h HBV DNA Ct值低于37℃下保存48 h、72 h,保存1周HBV DNA Ct值低于25℃、37℃下保存1周,差异有统计学意义(P<0.05);4℃、25℃下保存72 h、1周HBV DNA Ct值低于37℃下保存72 h、1周,差异有统计学意义(P<0.05)。2 mmol/L<甘油三酯(TG)<4 mmol/L组、4 mmol/L≤TG<6 mmol/L组、TG≥6 mmol/L组及对照组HBV DNA Ct值比较,差异无统计学意义(P>0.05)。20 g/L<血红蛋白(Hb)<40 g/L组、40 g/L≤Hb<80 g/L组及对照组HBV DNA检出率均高于Hb≥80 g/L组,差异有统计学意义(P<0.05)。20 g/L<Hb<40 g/L组、40 g/L≤Hb<80 g/L组、Hb≥80 g/L组及对照组HBV DNA Ct值比较,差异无统计学意义(P>0.05)。结论:在检测血液中HBV DNA时,应避免较高温度下长时间保存,同时脂血及轻中度溶血对HBV DNA检测影响较小,但重度溶血会导致HBV DNA检测假阴性结果的出现,因此对于重度溶血患者应重新抽血复检。展开更多
Two new copper complexes based on 2-naphthoxyacetic acid ligand, namely [Cu(L)2(CH3CN)]2(1) and [Cu(L)(1,10-phen)2](2), where L = 2-naphthoxyacetic acid and 1,10-phen = 1,10-phenanthroline, were obtained by hydrotherm...Two new copper complexes based on 2-naphthoxyacetic acid ligand, namely [Cu(L)2(CH3CN)]2(1) and [Cu(L)(1,10-phen)2](2), where L = 2-naphthoxyacetic acid and 1,10-phen = 1,10-phenanthroline, were obtained by hydrothermal reaction and characterized by single-crystal X-ray diffraction. The binuclear complex 1 and mononuclear complex 2 belong to space group C2/c and P■, respectively. The binding properties of the two compounds with ct-DNA were investigated by UV-Vis and fluorescence spectra. The two compounds could bind with ct-DNA through interactions. Compound 2 displays stronger binding ability in the reaction with ct-DNA.展开更多
A new complex Mn(Htpc)2(H2O)2(1, Htpc = 5-(trifluoromethyl)pyridine-2-carboxylic acid) has been synthesized and characterized by elemental analysis, IR, TG and single-crystal X-ray diffraction. 1 belongs to triclinic ...A new complex Mn(Htpc)2(H2O)2(1, Htpc = 5-(trifluoromethyl)pyridine-2-carboxylic acid) has been synthesized and characterized by elemental analysis, IR, TG and single-crystal X-ray diffraction. 1 belongs to triclinic system, space group P■ with a = 5.0885(10), b = 6.5574(13), c = 14.016(3) ?, β = 90.67(3)o, V = 436.34(17) ?3, Z = 1, Dc = 1.793 g·cm-3, μ = 0.855 mm-1, Mr = 471.18, F(000) = 235, the final R = 0.0454 and wR = 0.1134 for 1998 observed reflections with I > 2σ(I). The Mn(Ⅱ) ion is coordinated by two N and two O atoms from two Htpc as well as two O atoms from two coordinated water molecules, forming a 0D motif with distorted octahedral coordinate geometry. The adjacent 0D units are linked into 1D chains through hydrogen bond O(1W)–H(1 WB)···O(2), and via the O(1 W)–H(1 WA)···O(1) hydrogen bond the neighboring 1D chains are connected into a 2D supramolecular layer. Moreover, the interactions between the ligand and its complex with CT-DNA were studied by EtBr fluorescence probe, which suggested that these compounds bind to CT-DNA through an intercalation mode. The binding constants were 0.41 and 0.64 for Htpc and complex 1, respectively. It indicates that the interaction between complex 1 and CT-DNA is stronger than Htpc.展开更多
The protective roles of α-lipoic acid in the rat model of mitochondrial DNA (mtDNA) 4834bp deletion in inner ear were investigated. Forty female Wistar rats at 4 weeks of age were divided into four groups: group A (D...The protective roles of α-lipoic acid in the rat model of mitochondrial DNA (mtDNA) 4834bp deletion in inner ear were investigated. Forty female Wistar rats at 4 weeks of age were divided into four groups: group A (D-galactose group, n=10), group B (D-galactose+α-lipoic acid group, n=10), group C (α-lipoic acid group, n=10), and group D (control group, n=10). Auditory brainstem response (ABR) was used to detect the hearing threshold. Colorimetry was used to analyze activity of superoxide dismutase (SOD) and concentration of malondialdehyde (MDA). The percentage of mtDNA4834bp deletion in inner ear was identified by real-time PCR. There was no significant difference in ABR threshold shift among all groups. The percentage of mtDNA4834bp deletion in group A was higher than that in other groups, but there was no significant difference in percentage of mtDNA4834bp deletion among groups B, C, and D. The activity of SOD in group A was lower than that in other groups. The concentration of MDA in group A was higher than that in other groups. It was concluded that there was no significant hearing loss when the percentage of mtDNA4834bp deletion was lower than 12.5%. α-Lipoic acid could prevent the reactive oxygen species (ROS)-induced mtDNA4834bp deletion in inner ear of rats.展开更多
O6-methylguanine DNA methyltransferase(MGMT), a DNA repair enzyme, has been reported in some congenital malformations, but it is less frequently reported in neural tube defects. This study investigated MGMT mRNA expre...O6-methylguanine DNA methyltransferase(MGMT), a DNA repair enzyme, has been reported in some congenital malformations, but it is less frequently reported in neural tube defects. This study investigated MGMT mRNA expression and methylation levels in the early embryo and in different embryonic stages, as well as the relationship between MGMT and neural tube defects. Spina bifida aperta was induced in rats by a single intragastric administration of all-trans retinoic acid on embryonic day(E) 10, whereas normal control rats received the same amount of olive oil on the same embryonic day. DNA damage was assessed by detecting γ-H2 A.X in spina bifida aperta rats. Real time-polymerase chain reaction was used to examine mRNA expression of MGMT in normal control and spina bifida aperta rats. In normal controls, the MGMT mRNA expression decreased with increasing embryonic days, and was remarkably reduced from E11 to E14, reaching a minimum at E18. In the spina bifida aperta model, γ-H2 A.X protein expression was increased, and mRNA expression of MGMT was markedly decreased on E14, E16, and E18. Bisulfite sequencing polymerase chain reaction for MGMT promoter methylation demonstrated that almost all CpG sites in the MGMT promoter remained unmethylated in both spina bifida aperta rats and normal controls, and there was no significant difference in methylation level between the two groups on either E14 or E18. Our results show that DNA damage occurs in spina bifida aperta rats. The mRNA expression of MGMT is downregulated, and this downregulation is independent of promoter DNA methylation.展开更多
文摘Introduction: Transitioning to more efficacious Antiretrovirals for HIV infected pregnant women and infant prophylaxis has reduced Mother to child transmission of HIV significantly. This study aimed to determine HIV infection status in HIVexposed infants who had their first DNA polymerase chain reaction test in our molecular Laboratory. Subjects, Materials and Methods: Dried Blood Spots for HIV DNA results from 5 states between 2009 and 2020 were analyzed in the PCR laboratory of the Federal Teaching Hospital, Gombe. Results: Nine thousand eight hundred and twenty-three Human Immunodeficiency Virus Deoxyribonucleic acid polymerase Chain Reaction results were analysed;4937 (50.2%) were males. During the study period, there was an overall declining trend in the mother-to-child transmission rate from 3.8% in 2009 to 1.0% in 2020. 6120 (62.3%) of HIV + mothers received Highly active antiretroviral therapy HAART before pregnancy. 7845 (76.2%) of the infants received Nevirapine prophylaxis. Dried blood spot samples were collected from 4077 (41.5%) at 6 - 8 weeks. 8438 (85.9%) received cotrimoxazole. 9469 (96.4%) were ever breastfed. Of the 9823 HIV DNA PCR results, 255 (2.6%) were positive while 69/4077 (1.7%) and 109/2662 (4.1%) were positive for HIV DNA at 6 - 8 weeks and > 12 weeks respectively. (p = 0.001). 86/747 (11.5%) of infants whose HIV-positive mothers received no ARVS were HIV DNA positive. (p = 0.001). 106/884 (12.0%) of infants who had no Antiretroviral prophylaxis had positive HIV DNA results;7/413 (1.7%) with Zidovudine/Nevirapine prophylaxis had positive results. (p = 0.001). 246/9469 (2.6%) of infants that were ever breastfed were positive for HIV DNA;11/354 (3.0%) that never breastfed had positive HIV DNA. Conclusion: Lack of maternal/infant ARVs and prolonged breastfeeding increased the risk of infant HIV infection.
文摘Theoretical analysis of the DNA dynamics reveals that interaction between the single solitons and plane wave implies Smale-horseshoe chaos in the double helices. Solutions of the chaotic solitons are derived from a direct perturbation technique. It is demonstrated that to produce the bounded chaotic solitons, velocities of the solit ons nust be the same and equal to propagation velocity of the plane wave in DNA. The result shows that the DNA structure may be destroyed by the long action of an electromagnetic wave. It also supplies a useful method for controlling the velocities and unboundedness of the DNA motion in a tumour cell by using a plane wave.
文摘目的:分析不同保存温度及溶血、脂血血液标本对乙型肝炎病毒(HBV)脱氧核糖核酸(DNA)检测结果的影响。方法:选取2021年1-12月甘肃省肿瘤医院检验科的84份脂血血液标本、127份溶血血液标本及46份性状正常的血液标本分别作为脂血组、溶血组及对照组。检测对照组不同保存温度不同保存时间HBV DNA Ct值。检测脂血组及对照组标本中的HBV DNA Ct值。检测溶血组及对照组所有血液标本HBV DNA Ct值。比较对照组不同保存温度下4 h、24 h、48 h、72 h及1周的HBV DNA Ct值。比较对照组及脂血组的HBV DNA Ct值及HBV DNA检出率。比较对照组及溶血组的HBV DNA Ct值及HBV DNA检出率。结果:25℃下保存1周HBV DNA Ct值高于25℃下保存4 h、24 h、48 h、72 h,差异有统计学意义(P<0.05);37℃下保存72 h、1周HBV DNA Ct值高于37℃下保存4 h、24 h、48 h,差异有统计学意义(P<0.05);37℃下保存48 h HBV DNA Ct值高于37℃下保存4 h、24 h,差异有统计学意义(P<0.05)。-30℃下保存48 h、72 h HBV DNA Ct值低于37℃下保存48 h、72 h,保存1周HBV DNA Ct值低于25℃、37℃下保存1周,差异有统计学意义(P<0.05);4℃、25℃下保存72 h、1周HBV DNA Ct值低于37℃下保存72 h、1周,差异有统计学意义(P<0.05)。2 mmol/L<甘油三酯(TG)<4 mmol/L组、4 mmol/L≤TG<6 mmol/L组、TG≥6 mmol/L组及对照组HBV DNA Ct值比较,差异无统计学意义(P>0.05)。20 g/L<血红蛋白(Hb)<40 g/L组、40 g/L≤Hb<80 g/L组及对照组HBV DNA检出率均高于Hb≥80 g/L组,差异有统计学意义(P<0.05)。20 g/L<Hb<40 g/L组、40 g/L≤Hb<80 g/L组、Hb≥80 g/L组及对照组HBV DNA Ct值比较,差异无统计学意义(P>0.05)。结论:在检测血液中HBV DNA时,应避免较高温度下长时间保存,同时脂血及轻中度溶血对HBV DNA检测影响较小,但重度溶血会导致HBV DNA检测假阴性结果的出现,因此对于重度溶血患者应重新抽血复检。
基金supported by the National Natural Science Foundation of China(21101090 and 21561021)
文摘Two new copper complexes based on 2-naphthoxyacetic acid ligand, namely [Cu(L)2(CH3CN)]2(1) and [Cu(L)(1,10-phen)2](2), where L = 2-naphthoxyacetic acid and 1,10-phen = 1,10-phenanthroline, were obtained by hydrothermal reaction and characterized by single-crystal X-ray diffraction. The binuclear complex 1 and mononuclear complex 2 belong to space group C2/c and P■, respectively. The binding properties of the two compounds with ct-DNA were investigated by UV-Vis and fluorescence spectra. The two compounds could bind with ct-DNA through interactions. Compound 2 displays stronger binding ability in the reaction with ct-DNA.
基金Supported by the Scientific Research Foundation of Higher Education Institutions of Ningxia(No.NGY2017004)the National Natural Science Foundation of China(Nos.21763022 and 50564043)the Major Innovation Projects for Building First-class Universities in China’s Western Region(No.ZKZD2017003)
文摘A new complex Mn(Htpc)2(H2O)2(1, Htpc = 5-(trifluoromethyl)pyridine-2-carboxylic acid) has been synthesized and characterized by elemental analysis, IR, TG and single-crystal X-ray diffraction. 1 belongs to triclinic system, space group P■ with a = 5.0885(10), b = 6.5574(13), c = 14.016(3) ?, β = 90.67(3)o, V = 436.34(17) ?3, Z = 1, Dc = 1.793 g·cm-3, μ = 0.855 mm-1, Mr = 471.18, F(000) = 235, the final R = 0.0454 and wR = 0.1134 for 1998 observed reflections with I > 2σ(I). The Mn(Ⅱ) ion is coordinated by two N and two O atoms from two Htpc as well as two O atoms from two coordinated water molecules, forming a 0D motif with distorted octahedral coordinate geometry. The adjacent 0D units are linked into 1D chains through hydrogen bond O(1W)–H(1 WB)···O(2), and via the O(1 W)–H(1 WA)···O(1) hydrogen bond the neighboring 1D chains are connected into a 2D supramolecular layer. Moreover, the interactions between the ligand and its complex with CT-DNA were studied by EtBr fluorescence probe, which suggested that these compounds bind to CT-DNA through an intercalation mode. The binding constants were 0.41 and 0.64 for Htpc and complex 1, respectively. It indicates that the interaction between complex 1 and CT-DNA is stronger than Htpc.
基金supported by grants from the State Key Program of National Natural Science of China (No. 30730094)the National Science & Technology Pillar Program during the Eleventh Five-year Plan Period (No. 2007BAI18B13)
文摘The protective roles of α-lipoic acid in the rat model of mitochondrial DNA (mtDNA) 4834bp deletion in inner ear were investigated. Forty female Wistar rats at 4 weeks of age were divided into four groups: group A (D-galactose group, n=10), group B (D-galactose+α-lipoic acid group, n=10), group C (α-lipoic acid group, n=10), and group D (control group, n=10). Auditory brainstem response (ABR) was used to detect the hearing threshold. Colorimetry was used to analyze activity of superoxide dismutase (SOD) and concentration of malondialdehyde (MDA). The percentage of mtDNA4834bp deletion in inner ear was identified by real-time PCR. There was no significant difference in ABR threshold shift among all groups. The percentage of mtDNA4834bp deletion in group A was higher than that in other groups, but there was no significant difference in percentage of mtDNA4834bp deletion among groups B, C, and D. The activity of SOD in group A was lower than that in other groups. The concentration of MDA in group A was higher than that in other groups. It was concluded that there was no significant hearing loss when the percentage of mtDNA4834bp deletion was lower than 12.5%. α-Lipoic acid could prevent the reactive oxygen species (ROS)-induced mtDNA4834bp deletion in inner ear of rats.
基金supported by the National Natural Science Foundation of China,No.81671469,81171072(to ZWY)the National Basic Research Program of China(973 Program),No.2013CB945402(to ZWY)the Program for Liaoning Innovative Research Team in University of China,No.LT2013016(to ZWY)
文摘O6-methylguanine DNA methyltransferase(MGMT), a DNA repair enzyme, has been reported in some congenital malformations, but it is less frequently reported in neural tube defects. This study investigated MGMT mRNA expression and methylation levels in the early embryo and in different embryonic stages, as well as the relationship between MGMT and neural tube defects. Spina bifida aperta was induced in rats by a single intragastric administration of all-trans retinoic acid on embryonic day(E) 10, whereas normal control rats received the same amount of olive oil on the same embryonic day. DNA damage was assessed by detecting γ-H2 A.X in spina bifida aperta rats. Real time-polymerase chain reaction was used to examine mRNA expression of MGMT in normal control and spina bifida aperta rats. In normal controls, the MGMT mRNA expression decreased with increasing embryonic days, and was remarkably reduced from E11 to E14, reaching a minimum at E18. In the spina bifida aperta model, γ-H2 A.X protein expression was increased, and mRNA expression of MGMT was markedly decreased on E14, E16, and E18. Bisulfite sequencing polymerase chain reaction for MGMT promoter methylation demonstrated that almost all CpG sites in the MGMT promoter remained unmethylated in both spina bifida aperta rats and normal controls, and there was no significant difference in methylation level between the two groups on either E14 or E18. Our results show that DNA damage occurs in spina bifida aperta rats. The mRNA expression of MGMT is downregulated, and this downregulation is independent of promoter DNA methylation.