A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-(3-dimethylaminopropyl)-N’-et...A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride(EDC)and N-hydroxysuccinimide(NHS).By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25(n=8,γ=0.998 5)and the detection limit as 3.60×10-8 mol/L(3σ).展开更多
Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various biosensors.In particular,by exploiting the structural rigidity...Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various biosensors.In particular,by exploiting the structural rigidity and programmability of the framework nucleic acids with high precision,molecular recognition on the electrochemical biosensing interface has been significantly enhanced,leading to the development of highly sensitive and specific biosensors for nucleic acids,small molecules,proteins,and cells.In this review,we summarize recent advances in DNA framework-engineered biosensing interfaces and the application of corresponding electrochemical biosensors.展开更多
文摘A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride(EDC)and N-hydroxysuccinimide(NHS).By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25(n=8,γ=0.998 5)and the detection limit as 3.60×10-8 mol/L(3σ).
基金supported by the National Natural Science Foundation of China(21804091,21804088,21904086)Shanghai Pujiang Program(19PJ1407300)。
文摘Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various biosensors.In particular,by exploiting the structural rigidity and programmability of the framework nucleic acids with high precision,molecular recognition on the electrochemical biosensing interface has been significantly enhanced,leading to the development of highly sensitive and specific biosensors for nucleic acids,small molecules,proteins,and cells.In this review,we summarize recent advances in DNA framework-engineered biosensing interfaces and the application of corresponding electrochemical biosensors.