Recent studies suggest that Hypocretin (HCRT, Orexin) are involved in stress regulation of depression through the hypothalamic-pituitary-adrenal (HPA) axis. However, the molecular mechanism by which Hypocretin regulat...Recent studies suggest that Hypocretin (HCRT, Orexin) are involved in stress regulation of depression through the hypothalamic-pituitary-adrenal (HPA) axis. However, the molecular mechanism by which Hypocretin regulate neurobiological responses is unknown. Herein, the effects of chronic stress on the epigenetic modification of HCRT and its association with depression were explored with regard to a potential role in cancer progression. In the study, Sprague Dawley (SD) rats were used to establish an animal model of cancer with depression by administrating n-nitrosodiethylamine (DEN) and chronic unpredictable mild stress (CUMS). RNA-sequencing was used to detect differentially expressed genes in the hippocampus of rats and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the results of RNA-sequencing. The status of HCRT promoter methylation was assessed by methylation specific polymerase chain reaction. Behavioral tests showed that rats exposed to CUMS had significant depressive-like behaviors. The number of liver tumors and tumor load in depressed rats exposed to CUMS was higher than in SD rats without CUMS. RNA-sequencing revealed that HCRT was one of the most siginificantly downregulated gene in the hippocampus of SD rats with CUMS compared to non-stressed group, which was validated by qRT-PCR. HCRT mRNA expression was downregulated and the promoter for HCRT was hyper-methylated in those with depression. These results identified a critical role for chronic psychological stressors in tumorigenesis and cancer progression, via epigenetic HCRT downregulation. Such epigenetic downregulation may be the molecular basis for the association of cancer with depression.展开更多
Pore brine potassium and deep sand gravel layer found is of large size and high economic value liquid potash deposit in western Qaidam basin depression tectonic(Fig.1).Because the reservoir is relatively thick,it is d...Pore brine potassium and deep sand gravel layer found is of large size and high economic value liquid potash deposit in western Qaidam basin depression tectonic(Fig.1).Because the reservoir is relatively thick,it is difficult to展开更多
目的系统评价延续性护理对产后抑郁患者效果的影响。方法计算机检索Web of Science、PubMed、Embase、Cochrane Library、知网、万方、维普、生物医学文献数据库,收录自建库至2021年6月1日,已公开出版的关于延续性护理措施及其对产后抑...目的系统评价延续性护理对产后抑郁患者效果的影响。方法计算机检索Web of Science、PubMed、Embase、Cochrane Library、知网、万方、维普、生物医学文献数据库,收录自建库至2021年6月1日,已公开出版的关于延续性护理措施及其对产后抑郁症患者影响的相关文章,并通过RevMan 5.3软件进行meta分析。结果共纳入文献15篇,meta分析结果显示,实验组抑郁自评量表(SDS)评分低于对照组,差异有统计学意义[MD=-7.64,95%CI(-9.95,-5.33),P<0.00001];实验组焦虑自评量表(SAS)评分低于对照组,差异有统计学意义[MD=-9.27,95%CI(-11.06,-7.47),P<0.00001];实验组的产后抑郁发生率低于对照组,差异有统计学意义[OR=3.84,95%CI(1.84,8.03),P=0.0003];实验组的满意度优于对照组,差异有统计学意义[OR=8.48,95%CI(4.45,17.53),P<0.00001]。结论延续性护理措施能有效减轻产妇的抑郁不安心情,减少产后抑郁症发病率,可在临床推广应用。展开更多
基金The study was supported by the National Natural Science Foundation of China (NSFC) (No.#81872380)the Opening Foundation of Chongqing Key Laboratory of Molecular Oncology and Epigenetics.
文摘Recent studies suggest that Hypocretin (HCRT, Orexin) are involved in stress regulation of depression through the hypothalamic-pituitary-adrenal (HPA) axis. However, the molecular mechanism by which Hypocretin regulate neurobiological responses is unknown. Herein, the effects of chronic stress on the epigenetic modification of HCRT and its association with depression were explored with regard to a potential role in cancer progression. In the study, Sprague Dawley (SD) rats were used to establish an animal model of cancer with depression by administrating n-nitrosodiethylamine (DEN) and chronic unpredictable mild stress (CUMS). RNA-sequencing was used to detect differentially expressed genes in the hippocampus of rats and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the results of RNA-sequencing. The status of HCRT promoter methylation was assessed by methylation specific polymerase chain reaction. Behavioral tests showed that rats exposed to CUMS had significant depressive-like behaviors. The number of liver tumors and tumor load in depressed rats exposed to CUMS was higher than in SD rats without CUMS. RNA-sequencing revealed that HCRT was one of the most siginificantly downregulated gene in the hippocampus of SD rats with CUMS compared to non-stressed group, which was validated by qRT-PCR. HCRT mRNA expression was downregulated and the promoter for HCRT was hyper-methylated in those with depression. These results identified a critical role for chronic psychological stressors in tumorigenesis and cancer progression, via epigenetic HCRT downregulation. Such epigenetic downregulation may be the molecular basis for the association of cancer with depression.
文摘Pore brine potassium and deep sand gravel layer found is of large size and high economic value liquid potash deposit in western Qaidam basin depression tectonic(Fig.1).Because the reservoir is relatively thick,it is difficult to
文摘目的系统评价延续性护理对产后抑郁患者效果的影响。方法计算机检索Web of Science、PubMed、Embase、Cochrane Library、知网、万方、维普、生物医学文献数据库,收录自建库至2021年6月1日,已公开出版的关于延续性护理措施及其对产后抑郁症患者影响的相关文章,并通过RevMan 5.3软件进行meta分析。结果共纳入文献15篇,meta分析结果显示,实验组抑郁自评量表(SDS)评分低于对照组,差异有统计学意义[MD=-7.64,95%CI(-9.95,-5.33),P<0.00001];实验组焦虑自评量表(SAS)评分低于对照组,差异有统计学意义[MD=-9.27,95%CI(-11.06,-7.47),P<0.00001];实验组的产后抑郁发生率低于对照组,差异有统计学意义[OR=3.84,95%CI(1.84,8.03),P=0.0003];实验组的满意度优于对照组,差异有统计学意义[OR=8.48,95%CI(4.45,17.53),P<0.00001]。结论延续性护理措施能有效减轻产妇的抑郁不安心情,减少产后抑郁症发病率,可在临床推广应用。
