In order to explore researches about the chromosome karyotype analysis and fluorescence in situ hybridization(FISH) technology in China,using the bibliometric method,taking " fluorescence in situ hybridization(FI...In order to explore researches about the chromosome karyotype analysis and fluorescence in situ hybridization(FISH) technology in China,using the bibliometric method,taking " fluorescence in situ hybridization(FISH) " and " chromosome" as key words,this paper made a statistical analysis on the literature published in China National Knowledge Infrastructure(CNKI) during 2002-2016.The results indicated that the number of papers published in 2002 was the smallest(37),while the number of papers published in 2012 was the largest(125).In terms of the distribution of organizations of authors,in 1201 papers,11 organizations published papers ≥15,accounting for 21.65%.In terms of distribution of papers published by different periodicals,11 periodicals published papers ≥10,accounting for 17.65%.In terms of the papers supported by foundation projects,in all papers searched,377 papers were supported by foundation projects,accounting for 31.39%.In terms of the distribution of doctoral and master's dissertations,259 papers were master's dissertations,accounting for 21.57%;92 papers were doctoral dissertations,accounting for 7.66%.展开更多
It is now well known that levels of sperm disomy correlate to levels of infertility （as well as other factors）.The risk of perpetuating aneuploidy to the offspring of infertile males undergoing intracytoplasmic sper...It is now well known that levels of sperm disomy correlate to levels of infertility （as well as other factors）.The risk of perpetuating aneuploidy to the offspring of infertile males undergoing intracytoplasmic sperm injection （ICSI） has become a hotly debated issue in assisted reproduction;however,there remain barriers to the practical implementation of offering sperm disomy screening in a clinical setting. The major barrier is the operator time taken to analyze a statistically meaningful （sufficient） number of cells. The introduction of automated ＇spot counting＇software-hardware combinations presents a potential solution to this problem. In this preliminary validation study,we analyzed 10 patients,both manually and using a commercially available spot counter. Results show a statistically significant correlation between both approaches for scoring of sperm disomy,but no correlation is found when scoring for diploid sperm. The most likely explanation for the latter is an apparent overscoring of two closely associated sperm heads as a single diploid cell. These results,and similar further studies that will ensue,help to inform cost-benefit analyses that individual clinics need to carry out in order to decide whether to adopt sperm aneuploidy screening as a routine tool for the assessment of sperm from men requiring ICSI treatment.展开更多
为解决人工对荧光原位杂交(Fluorescence In Situ Hybridization,FISH)荧光图像进行结果判读存在的效率低、劳动强度大等问题,针对FISH荧光图像细胞智能检测提出一种融合空域图像增强的改进YOLOv5算法。算法在原始YOLOv5神经网络模型基...为解决人工对荧光原位杂交(Fluorescence In Situ Hybridization,FISH)荧光图像进行结果判读存在的效率低、劳动强度大等问题,针对FISH荧光图像细胞智能检测提出一种融合空域图像增强的改进YOLOv5算法。算法在原始YOLOv5神经网络模型基础上,加入了空域图像增强模块,并选择了模块最佳增强系数,扩大了模型对荧光图像的对比度适应范围,提高了模型的特征提取能力和细胞检测准确率。实验结果显示,改进YOLOv5模型的平均精度均值(Mean Average Precision,mAP)为0.983,达到了比原始模型更优的训练效果和收敛速度,并且,改进YOLOv5模型的细胞识别率达到91.65%,比原始YOLOv5模型提升了9.19%。将细胞智能检测算法嵌入自主开发的荧光图像智能检测软件,结合荧光点检测算法,可给出有效判读结果。展开更多
FISH(fluorescence in situ hybridization,荧光原位杂交)染色是应用于病理分析的重要技术。传统的手工染色方式由于操作繁琐及实验条件限制难以控制杂交质量,而国内现有的染色系统存在自动化程度低等缺点。为了解决上述问题,基于STM32...FISH(fluorescence in situ hybridization,荧光原位杂交)染色是应用于病理分析的重要技术。传统的手工染色方式由于操作繁琐及实验条件限制难以控制杂交质量,而国内现有的染色系统存在自动化程度低等缺点。为了解决上述问题,基于STM32嵌入式系统和GUI(graphical user interface,图形用户界面)开发了一套全自动病理染色控制系统。以STM32F103ZET6作为下位机处理器,利用QT软件和C++语言设计GUI界面,实现了对多样本FISH染色过程的全自动化控制。所研发的控制系统能够满足全自动病理染色系统的控制要求,滴加试剂的位置精度达到0.05 mm,试剂体积精度达到0.6μL,设备故障响应时间小于0.5 s,多次实验设备运行故障率小于3%,加样时试剂类型选择准确率达到100%,图像可判读率达到90%以上。该控制系统具有高效率、高鲁棒性的优点,结合全自动病理染色硬件系统进行染色实验,取得了均匀、良好的染色效果,因此具有良好的应用价值。展开更多
基金Supported by Project of National Natural Science Foundation(31160292)Crop Discipline Construction Project of Tibet Agricultural and Animal Husbandry College(2015ZWXKJS&2016ZWXKJS)
文摘In order to explore researches about the chromosome karyotype analysis and fluorescence in situ hybridization(FISH) technology in China,using the bibliometric method,taking " fluorescence in situ hybridization(FISH) " and " chromosome" as key words,this paper made a statistical analysis on the literature published in China National Knowledge Infrastructure(CNKI) during 2002-2016.The results indicated that the number of papers published in 2002 was the smallest(37),while the number of papers published in 2012 was the largest(125).In terms of the distribution of organizations of authors,in 1201 papers,11 organizations published papers ≥15,accounting for 21.65%.In terms of distribution of papers published by different periodicals,11 periodicals published papers ≥10,accounting for 17.65%.In terms of the papers supported by foundation projects,in all papers searched,377 papers were supported by foundation projects,accounting for 31.39%.In terms of the distribution of doctoral and master's dissertations,259 papers were master's dissertations,accounting for 21.57%;92 papers were doctoral dissertations,accounting for 7.66%.
文摘It is now well known that levels of sperm disomy correlate to levels of infertility （as well as other factors）.The risk of perpetuating aneuploidy to the offspring of infertile males undergoing intracytoplasmic sperm injection （ICSI） has become a hotly debated issue in assisted reproduction;however,there remain barriers to the practical implementation of offering sperm disomy screening in a clinical setting. The major barrier is the operator time taken to analyze a statistically meaningful （sufficient） number of cells. The introduction of automated ＇spot counting＇software-hardware combinations presents a potential solution to this problem. In this preliminary validation study,we analyzed 10 patients,both manually and using a commercially available spot counter. Results show a statistically significant correlation between both approaches for scoring of sperm disomy,but no correlation is found when scoring for diploid sperm. The most likely explanation for the latter is an apparent overscoring of two closely associated sperm heads as a single diploid cell. These results,and similar further studies that will ensue,help to inform cost-benefit analyses that individual clinics need to carry out in order to decide whether to adopt sperm aneuploidy screening as a routine tool for the assessment of sperm from men requiring ICSI treatment.
文摘为解决人工对荧光原位杂交(Fluorescence In Situ Hybridization,FISH)荧光图像进行结果判读存在的效率低、劳动强度大等问题,针对FISH荧光图像细胞智能检测提出一种融合空域图像增强的改进YOLOv5算法。算法在原始YOLOv5神经网络模型基础上,加入了空域图像增强模块,并选择了模块最佳增强系数,扩大了模型对荧光图像的对比度适应范围,提高了模型的特征提取能力和细胞检测准确率。实验结果显示,改进YOLOv5模型的平均精度均值(Mean Average Precision,mAP)为0.983,达到了比原始模型更优的训练效果和收敛速度,并且,改进YOLOv5模型的细胞识别率达到91.65%,比原始YOLOv5模型提升了9.19%。将细胞智能检测算法嵌入自主开发的荧光图像智能检测软件,结合荧光点检测算法,可给出有效判读结果。
文摘FISH(fluorescence in situ hybridization,荧光原位杂交)染色是应用于病理分析的重要技术。传统的手工染色方式由于操作繁琐及实验条件限制难以控制杂交质量,而国内现有的染色系统存在自动化程度低等缺点。为了解决上述问题,基于STM32嵌入式系统和GUI(graphical user interface,图形用户界面)开发了一套全自动病理染色控制系统。以STM32F103ZET6作为下位机处理器,利用QT软件和C++语言设计GUI界面,实现了对多样本FISH染色过程的全自动化控制。所研发的控制系统能够满足全自动病理染色系统的控制要求,滴加试剂的位置精度达到0.05 mm,试剂体积精度达到0.6μL,设备故障响应时间小于0.5 s,多次实验设备运行故障率小于3%,加样时试剂类型选择准确率达到100%,图像可判读率达到90%以上。该控制系统具有高效率、高鲁棒性的优点,结合全自动病理染色硬件系统进行染色实验,取得了均匀、良好的染色效果,因此具有良好的应用价值。
