It is found that in the presence of sulfuric acid carbohydrates condense withurea to afford the condensation products, which emit fluorescence. Under optimum conditions, thefluorescence intensities of system are propo...It is found that in the presence of sulfuric acid carbohydrates condense withurea to afford the condensation products, which emit fluorescence. Under optimum conditions, thefluorescence intensities of system are proportional to the concentrations of carbohydrates. Based onthis linear relationship, quantitative determination of kinds of carbohydrates has been made. Amongall the carbohydrates tested, the sensitivity of α-rhamnose is the highest and its limits ofdetection reaches 3. 5 * 10^(-8) mol/L. So α-rhamnose can be selectively determined in the presenceof other carbohydrates. A interaction mechanism is also discussed.展开更多
基金theNationalNaturalScienceFoundationsofChina (No .2 9875 0 15 ),theNaturalScienceFoundationofShandongProvince (No .Z97B0 10 0 2 ),andtheVisitingScholarFoundationofKeyLaboratoryinShandongUniversity
文摘It is found that in the presence of sulfuric acid carbohydrates condense withurea to afford the condensation products, which emit fluorescence. Under optimum conditions, thefluorescence intensities of system are proportional to the concentrations of carbohydrates. Based onthis linear relationship, quantitative determination of kinds of carbohydrates has been made. Amongall the carbohydrates tested, the sensitivity of α-rhamnose is the highest and its limits ofdetection reaches 3. 5 * 10^(-8) mol/L. So α-rhamnose can be selectively determined in the presenceof other carbohydrates. A interaction mechanism is also discussed.
