Effect of Cistanche Desertica Polysaccharides on Learning and Memory Functions and Ultrastructure of Cerebral Neurons in Experimental Aging Mice

To observe the effects of Cistanche desertica polysaccharides (CDP) on the learning and memory functions and cerebral ultrastructure in experimental aging mice. Methods: CDP was administrated intragastrically 50 or 100 mg/kg per day for 64 successive days to experimental aging model mice induced by D-galactose, then the learning and memory functions of mice were estimated by step-down test and Y-maze test; organelles of brain tissue and cerebral ultrastructure were observed by transmission electron microscope and physical strength was determined by swimming test. Results: CDP could obviously enhance the learning and memory functions (P＜0.01) and prolong the swimming time (P＜0.05), decrease the number of lipofuscin and slow down the degeneration of mitochondria in neurons(P＜0.05), and improve the degeneration of cerebral ultra-structure in aging mice. Conclusion: CDP could improve the impaired physiological function and alleviate cerebral morphological change in experimental aging mice.

实验性衰老小鼠肺功能和超微结构的变化及肉苁蓉多糖的影响

目的 观察实验性衰老小鼠肺组织细胞超微结构的退行性改变和肉苁蓉多糖对其的影响。方法 使用臭氧(Ozone,O3 )连续吸入 4wk致肺衰老模型 ,应用肉苁蓉多糖5 0 ,10 0mg·kg-1·d-1灌服模型小鼠 6wk ,检测衰老小鼠的耐缺氧能力、肺和血清中超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)含量 ,电镜下观察肺组织细胞器和超微结构的变化。结果 臭氧所致实验性衰老小鼠耐缺氧能力明显下降 ;肺和血清中SOD降低、MDA升高。与模型组比较 ,肉苁蓉多糖能明显延长衰老小鼠耐缺氧时间 (P <0 0 1) ,提高血清中SOD活力、降低MDA含量 (P <0 0 1) ,延缓衰老小鼠肺细胞线粒体、板层小体等超微结构的退变 (P <0 0 1)。结论 肉苁蓉多糖能明显延缓实验性衰老小鼠肺的生理性退化与细胞形态学退变。

肉苁蓉多糖对衰老小鼠肺蛋白含量与抗氧化功能关系的影响

使用D 半乳糖造成小鼠实验性衰老模型 ,观察肉苁蓉多糖对实验性衰老小鼠肺胶原蛋白、弹性蛋白含量的影响 ,对肺组织和红细胞中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)及丙二醛 (MDA)含量的影响。结果 灌服肉苁蓉多糖的衰老小鼠的肺组织及红细胞中SOD、GSH Px活力不同程度提高 ,肺及血浆中丙二醛含量有所降低。同时肺胶原蛋白含量减少 ,弹性蛋白含量增多。