Construction of the Plasmid Reference Molecule for Detection of Transgenic Soybean MON89788

[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.

Urchin-like Na-doped zinc oxide nanoneedles for low-concentration and exclusive VOC detections

In the early-stage diagnosis of lung cancer,the low-concentration(<5 ppm)volatile organic compounds(VOCs)are extensively identified to be the biomarkers for breath analysis.Herein,the urchin-like sodium(Na)-doped zinc oxide(ZnO)nanoneedles were synthesized through a hydrothermal strategy with the addition of different contents of citric acid.The Na-doped ZnO gas sensor with a 3:1 molar ratio of Na^(+)and citric acid showed outstanding sensing properties with an optimal selectivity to various VOCs(formaldehyde(HCOH),isopropanol,acetone,and ammonia)based on working temperature regulation.Specifically,significantly enhanced sensitivity(21.3@5 ppm)compared with pristine ZnO(~7-fold),low limit of detection(LOD)(298 ppb),robust humidity resistance,and long-term stability of formaldehyde sensing performances were obtained,which can be attributed to the formation of a higher concentration of oxygen vacancies(20.98%)and the active electron transitions.Furthermore,the improved sensing mechanism was demonstrated by the exquisite band structure and introduction of the additional acceptor level,which resulted in the narrowed bandgap of ZnO.

Upconversion luminescence turning of NaREF4(RE=0.4Y＋0.4La＋0.2(Yb, Er, Tm))nanoparticles and their applications for detecting Rhodamine B in shrimp

Biocompatible NaREF_4（RE=0.4Y＋0.4La＋0.2（Yb,Er,Tm）（molar ratio）） upconversion nanoparticles（UCNPs） with strong visible fluorescence were synthesized by a solvothermal method and subsequent surface modification. Modulated upconversion luminescence emission spectra were obtained via changing the doping. In vitro and in vivo bioimagings were carried out with shrimps. The upconversion nanoprobes with an acidic/PEG hybrid ligand could quickly capture the basic Rhodamine-B（RB） in shrimp cells and formed a close UCNPs@RB system. The residual organic dye RB in shrimps could be detected on the basis of luminescent resonance energy transfer（LRET）. It could be rapidly addressed based on LRET detection that RB residue existed in the shrimps after incubating in the aqueous solution of RB higher than 3 μg/m L for 12 h.