One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development.Availability of chromosome arm substitution line CS-
A male sterile mutant plant was found from the rice field of the local rice variety which was cultivated for a long time in the west of Hubei Province. The plant has more vigorous tillering ability. Its plant height o...A male sterile mutant plant was found from the rice field of the local rice variety which was cultivated for a long time in the west of Hubei Province. The plant has more vigorous tillering ability. Its plant height of 95 cm was a bit shorter than that of the normal ones. The plant type, the leaf shape and the color of leaf sheath and glume tip of this mutant were identieal to that of the normal plant whereas the color of arrowhead-shape anther was milky white. This mutant set no seed during self-pollination. When it was crossed with IR24. IR26, Minhui 63 or Xieqing-zaoxuian. the Ffertilities were normal. The fertility of this mutant could be restored by IR24, IR26 and Minhui 63 and maintained by Xieqing-zaoxian. Maxie A was developed in 1987 by the successive backcrossing of the mutant with Xieqing-zaoxian. The percentage of abortive pollen in Maxie A in uninucleate microspore to early binucleate microspore stage is展开更多
The striatum, as the primary input nucleus in the basal ganglion,plays an important role in neural circuits crucial for the control of critical motivation, motor planning and procedural learning(Kreitzer and Malenka, ...The striatum, as the primary input nucleus in the basal ganglion,plays an important role in neural circuits crucial for the control of critical motivation, motor planning and procedural learning(Kreitzer and Malenka, 2008). Most cells in the striatum are GABAergic, including a large population (90%-95%) of medium spiny neurons (MSNs) and a small population of interneurons.展开更多
Promoting regional coordinated development strategy is one of the important strategies in the new period of China.Faced with the reality of unbalanced and insufficient regional development in China,it is objectively n...Promoting regional coordinated development strategy is one of the important strategies in the new period of China.Faced with the reality of unbalanced and insufficient regional development in China,it is objectively necessary to construct one or more main axes supporting the coordinated and balanced development of regions to become the identification line representing the pattern of coordinated regional development.The results show that the Bo-Tai line,the northwest-southeast axis connecting Bole of Xinjiang and Taipei of Taiwan,can be built into national development backbone line and regional balanced development line,just perpendicular to Hu Line.In 2016,the area of southwest half and northeast half of Bo-Tai Line accounts for 60%:40%,while the population accounts for 45%:55%,the economic aggregate accounts for 40%:60%,the per capita GDP ratio accounts for 44%:56%,the population density ratio accounts for 38%:62%,the economic density ratio accounts for 32%:68%,and the urbanization level ratio accounts for 48%:52%.The main average indicators are gradually tending to balanced development pattern.Further analysis shows that Bo-Tai Line is a strategic shoulder pole connecting two core zones of"the Belt and Road",and is the peaceful reunification line of China's national tranquility and Taiwan's return.Bo-Tai Line is also a solid line supported and connected by comprehensive transportation channels and a Pipa type symmetrical line for the development of cities and urban agglomerations.It is the backbone of the two-way opening up and the linkage development line between land and sea.It is also an important dividing line that promotes the coordinated development of the eastern,central and western regions,and addresses the imbalance and inadequacy of regional development.Bo-Tai Line plays an irreplaceable strategic role in promoting the coordinated and balanced regional development.It is suggested that the construction of Bo-Tai Line should be included in the national development strategy,and the development strategic plan of Bo-Tai Line should be formulated to fully release the multiple potential functions.We should build three strategic support points:the northwest endpoint,the central strategic node and the southeast endpoint;carry out a comprehensive scientific investigation of the Bo-Tai Line,and strengthen the scientific cognition and publicity;promote China's development in a higherlevel,higher-quality,more coordinated,safer and more civilized direction.Let Chinese know about the Bo-Tai Line,let the world know about the Bo-Tai Line,and let the Bo-Tai Line truly become the backbone of the great rejuvenation of the Chinese nation.展开更多
THE first cytoplasmic male sterile soybean line was reported by Davis in a U.S. Patent, but no further information has been released since then. Sun et al. developed a cytoplasmic-nuclear male sterile soy-
The Chinese Hamster Ovary （CHO K1） cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was fi...The Chinese Hamster Ovary （CHO K1） cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase （GS） vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the produc- tion of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methio- nine sulfoximine. The best cell pool ofCHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.展开更多
文摘One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development.Availability of chromosome arm substitution line CS-
文摘A male sterile mutant plant was found from the rice field of the local rice variety which was cultivated for a long time in the west of Hubei Province. The plant has more vigorous tillering ability. Its plant height of 95 cm was a bit shorter than that of the normal ones. The plant type, the leaf shape and the color of leaf sheath and glume tip of this mutant were identieal to that of the normal plant whereas the color of arrowhead-shape anther was milky white. This mutant set no seed during self-pollination. When it was crossed with IR24. IR26, Minhui 63 or Xieqing-zaoxuian. the Ffertilities were normal. The fertility of this mutant could be restored by IR24, IR26 and Minhui 63 and maintained by Xieqing-zaoxian. Maxie A was developed in 1987 by the successive backcrossing of the mutant with Xieqing-zaoxian. The percentage of abortive pollen in Maxie A in uninucleate microspore to early binucleate microspore stage is
基金supported by grants from the National Key Research and Development Program of China (2016YFA0100702 and 2016YFC0902502)the National Natural Science Foundation of China (31670789 and 31671316)the CAMS Innovation Fund for Medical Sciences (CIFMS, 2016I2M-2-001, 2016-I2M-1-001, 2016-I2M-1-004 and 2017-I2M-1004)
文摘The striatum, as the primary input nucleus in the basal ganglion,plays an important role in neural circuits crucial for the control of critical motivation, motor planning and procedural learning(Kreitzer and Malenka, 2008). Most cells in the striatum are GABAergic, including a large population (90%-95%) of medium spiny neurons (MSNs) and a small population of interneurons.
基金Major Program of the National Natural Science Foundation of China,No.41590840,No.41590842。
文摘Promoting regional coordinated development strategy is one of the important strategies in the new period of China.Faced with the reality of unbalanced and insufficient regional development in China,it is objectively necessary to construct one or more main axes supporting the coordinated and balanced development of regions to become the identification line representing the pattern of coordinated regional development.The results show that the Bo-Tai line,the northwest-southeast axis connecting Bole of Xinjiang and Taipei of Taiwan,can be built into national development backbone line and regional balanced development line,just perpendicular to Hu Line.In 2016,the area of southwest half and northeast half of Bo-Tai Line accounts for 60%:40%,while the population accounts for 45%:55%,the economic aggregate accounts for 40%:60%,the per capita GDP ratio accounts for 44%:56%,the population density ratio accounts for 38%:62%,the economic density ratio accounts for 32%:68%,and the urbanization level ratio accounts for 48%:52%.The main average indicators are gradually tending to balanced development pattern.Further analysis shows that Bo-Tai Line is a strategic shoulder pole connecting two core zones of"the Belt and Road",and is the peaceful reunification line of China's national tranquility and Taiwan's return.Bo-Tai Line is also a solid line supported and connected by comprehensive transportation channels and a Pipa type symmetrical line for the development of cities and urban agglomerations.It is the backbone of the two-way opening up and the linkage development line between land and sea.It is also an important dividing line that promotes the coordinated development of the eastern,central and western regions,and addresses the imbalance and inadequacy of regional development.Bo-Tai Line plays an irreplaceable strategic role in promoting the coordinated and balanced regional development.It is suggested that the construction of Bo-Tai Line should be included in the national development strategy,and the development strategic plan of Bo-Tai Line should be formulated to fully release the multiple potential functions.We should build three strategic support points:the northwest endpoint,the central strategic node and the southeast endpoint;carry out a comprehensive scientific investigation of the Bo-Tai Line,and strengthen the scientific cognition and publicity;promote China's development in a higherlevel,higher-quality,more coordinated,safer and more civilized direction.Let Chinese know about the Bo-Tai Line,let the world know about the Bo-Tai Line,and let the Bo-Tai Line truly become the backbone of the great rejuvenation of the Chinese nation.
文摘THE first cytoplasmic male sterile soybean line was reported by Davis in a U.S. Patent, but no further information has been released since then. Sun et al. developed a cytoplasmic-nuclear male sterile soy-
文摘The Chinese Hamster Ovary （CHO K1） cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase （GS） vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the produc- tion of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methio- nine sulfoximine. The best cell pool ofCHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.
