Temporal and environmental factors drive community structure and function of methanotrophs in volcanic forest soils

Methanotrophs,organisms that obtain oxygen by oxidizing methane,are recognized as the only known biological sink for atmospheric CH_4,and forest soil methanotrophs play crucial roles in mitigating global warming.The succession patterns of methanotrophic communities and functions in Wudalianchi volcano forest soils could provide a basis for the study of evolutionary mechanisms between soil microorganisms,the environment,and carbon cycling of temperate forest ecosystems under climate change.In this study,the characteristics and drivers of methanotrophic community structure and function of two volcanic soils at different stages of development are analyzed,including an old volcano and a new volcano,which most recently erupted 300 years and 17-19×10^(5)years ago,respectively,and a non-volcano hills as control,based on space for time substitution and Miseq sequencing and bioinformation technology.The results showed that CH_(4) fluxes were significantly higher in old-stage volcano forest soils than new-stage forest soils and non-volcano forest soils.There were significant differences in the community composition and diversity of soil methanotrophs from different volcano forest soils.Methylococcus was the dominant genus in all soil samples.Additionally,the relative abundance of Methylococcus,along with Clonothrix,Methyloglobulus,Methylomagum,Methylomonas and Methylosarcina,were the important genera responsible for the differences in methanotrophic community structure in different volcano forest soils.The relative abundance of methanotroph belonging toγ-proteobacteria was significantly higher than that belonging toα-proteobacteria(P<0.05).Chao1,Shannon and Simpson indices of soil methanotrophic community were significantly lower in new-stage volcanos and were significantly affected by bulk density,total porosity,p H,nitrate,dissolved organic carbon and dissolved organic nitrogen.There were significant differences in community structure between new-stage and old-stage volcanoes.Bulk density and p H are important soil properties contributing to the divergence of methanotrophs community structure,and changes in soil properties due to soil development time are important factors driving differences in methanotrophs communities in Wudalianchi volcanic soils.

Developing a process-based and remote sensing driven crop yield model for maize(PRYM–Maize) and its validation over the Northeast China Plain

Spatial dynamics of crop yield provide useful information for improving the production. High sensitivity of crop growth models to uncertainties in input factors and parameters and relatively coarse parameterizations in conventional remote sensing(RS) approaches limited their applications over broad regions. In this study, a process-based and remote sensing driven crop yield model for maize(PRYM–Maize) was developed to estimate regional maize yield, and it was implemented using eight data-model coupling strategies(DMCSs) over the Northeast China Plain(NECP). Simulations under eight DMCSs were validated against the prefecture-level statistics(2010–2012) reported by National Bureau of Statistics of China, and inter-compared. The 3-year averaged result could give more robust estimate than the yearly simulation for maize yield over space. A 3-year averaged validation showed that prefecture-level estimates by PRYM–Maize under DMCS8, which coupled with the development stage(DVS)-based grain-filling algorithm and RS phenology information and leaf area index(LAI), had higher correlation(R, 0.61) and smaller root mean standard error(RMSE, 1.33 t ha^(–1)) with the statistics than did PRYM–Maize under other DMCSs. The result also demonstrated that DVS-based grain-filling algorithm worked better for maize yield than did the harvest index(HI)-based method, and both RS phenology information and LAI worked for improving regional maize yield estimate. These results demonstrate that the developed PRYM–Maize under DMCS8 gives reasonable estimates for maize yield and provides scientific basis facilitating the understanding the spatial variations of maize yield over the NECP.

Developing ISO International Standards for the Animal and Vegetable Fats and Oils Sector

Increasing volumes of oils and fats are produced annually with a large section that are traded internationally. The International Organization for Standardization(ISO) body develops international standards covering almost all aspects of technology and manufacturing with 23,882 standards currently published. Standards in the animal and vegetable fats and oils sector is developed by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal and vegetable fats and oils. Standards are developed through consensus by national members that represent ISO in their country. Each Standard is developed within a determined timeframe and goes through various pre-determined stages such as project proposal, development, national voting and commenting on the draft Standard to final publication. ISO TC34/SC11 Standards range from sampling of oils and fats, sample preparation to analysis of its composition, the minor components, and quality and identity parameters as well as contaminants. The analysis results are used by industry for product development, labelling, research and to provide peace of mind for consumers on its safety. An important future trend is the focus on the safety concerns from either environmental contamination(e.g. mineral oils) or processing contaminants such as esters of 3-MCPDs(3-monochloropropane-1,2-diol or 3-chloropropane-1,2-diol).

Three Development Stages for the Tibetan Family and Tibet's Modernization——A Survey of Social Changes in Tibet in Last 50 Years

I Since 1990, as a person in charge of key research projects of the China Tibetology Research Center, I have had opportunities almost every year to conduct field surveys on anthropology in Tibet together with my colleagues. Our investigations focus on the family. The purpose of our surveys is to see social changes in Tibet during the 50 years after the Democratic Reform through the study of the family as a social cell.

Production evolution patterns and development stage division of waterflooding oilfields

The continuous growth of recoverable reserves in a waterflooding oilfield has a significant impact on the patterns of production evolution. A new production evolution model is established by improving the Weng Cycle model. With the new model, the statistical correspondence between the production decline stage and the reserve-production imbalance is clarified,and the correlation of water cut with the recovery percent of recoverable reserves is discussed, providing quantitative basis of reservoir engineering for dividing development stages of oilfield and defining mature oilfields. According to the statistics of oilfields in eastern China, the time point corresponding to the reserve-production balance coefficient dropping to less than 1dramatically is well correlated the beginning point of production decline, thus the time when the reserve-production balance coefficient drops dramatically can be taken as the initiation point of production decline stage. The research results show that the water cut and the recovery percent of recoverable reserves have a good statistical match in the high water cut stage, and it is more rational to take both the start point of production decline stage and the water cut of 90%(or the recovery percent of recoverable reserves of 80%) as the critical criteria for defining a mature oilfield. Five production evolution patterns can be summarized as follows: growth–peak plateau–stepped decline, growth–stepped stabilizing–stepped decline, growth–stepped stabilizing–rapid decline, growth–peak plateau–rapid decline, and growth–continuous decline.

Trend of global carbon dioxide capture,utilization and storage industry and challenges and countermeasures in China

This paper systematically reviews the trend of carbon dioxide capture,utilization and storage(CCUS)industry in the world and China,presents the CCUS projects,clusters,technologies and strategies/policies,and analyzes the CCUS challenges and countermeasures in China based on the comparison of CCUS industrial development at home and abroad.The global CCUS development has experienced three stages:exploration stage,policy driven stage,and dual-drive stage.Currently,the active large-scale CCUS projects around the world focus on enhanced oil recovery(EOR)and are expanding into storage in saline aquifers.The CCUS industry of China has evolved in three stages:exploration,pilot test,and industrialization.In the current critical period of transition from field test to industrialization,China’s CCUS projects are EOR-dominated.By comparing the industrial development of CCUS in China and abroad,it is found that the scale-up and industrialization of CCUS in China face challenges in technology,facilities and policies.Finally,future solutions to CCUS development in China are proposed as follows:strengthening the top-level design and planning of CCUS;developing high-efficiency and low-cost CCUS technologies throughout the whole industrial chain;deploying CCUS oil and gas+new energy clusters;improving the policy support system of CCUS;and strengthening discipline construction and personnel training,etc.

Construction of China Osmanthus City and Intellectual Property Right Protection in Xianning City in the Context of Three New Developments

Xianning City of Hubei Province is one of the five major traditional osmanthus producing areas in the world and the hometown of osmanthus in China. Xianning is constructing China Osmanthus City in the whole city. This paper first introduces the main characteristics of the osmanthus industry in Xianning City, and analyzes the characteristics of traditional knowledge, biological genetic resources, new plant varieties, patents, geographical indications, and trademarks. Then, it studies the intellectual property resources of the osmanthus industry, and analyzes the main problems existing in the construction of China Osmanthus City and intellectual property right protection. Finally, it comes up with constructive recommendations including applying for China and Globally Important Agricultural Heritage Systems, developing colored-leaf osmanthus varieties, creating a regional public brand of osmanthus specialty products, and building a new development pattern of osmanthus industry.

Developmetn stage threshold of watershed Landforms in Loess Plateau and separation of erosion mechanism

Threshold is a limit and marginal point when a qualitative change takes place. Since threshold principle was firstly used in landform research by S.A.Schumm in the 1970s, the quantitative research on watershed landform development stage has come true. Davis proposed a three-stage model on landform evolution: young, mature and old stages. Thereafter Strahler quantified this model by hypsometric analysis method. The authors thought that the material movement stage cannot be expressed by hypsometric method in watershed landform at development stage, because of the uncertainty on stage delimitation. To meet this shortcoming, this paper presents an integral erosion value method. A clear delimitation on landform development stage in the Loess Plateau region has been tested by this method. The result shows that gullied loessial hilly area is at the mature stage, and gullied loessial tableland area is at the young stage. It is estimated that from the point of erosion related sediment yield, natural erosion accounts for 70% of the total erosion amount, and artificial accelerating erosion is 30%. Therefore soil and water conservation is very crucial for the Loess Plateau.

Early Growth and Development of Boer Goats from Embryo Transplant

[ Objective] To study the growth-development of Boer goats from embryo transplant. [ Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK, the weight of Boer goats at birth and at the age of 1,3 and 6 months as well as the body size at the age of 3 and 6 months were investigated in this study, while their health conditions were also observed and recorded. [ Result] Under the normal feeding conditions, Boer goats from embryo transplant developed well at early stage, and their meat-using body type were also obvious. The average birth weight of bucks was （4.25±0.95） kg, and that of does was （3.74±0.10） kg. The average weight of bucks at the age of 6 months was （31.90 ±0.74） kg and that of was （25.90 ±0.67） kg. The daily gains of bucks and ewes from birth to 6 months of age were 153.64 and 123.11 g respectively, while their body length, body height and chest circumference increased obviously, and there was no significant difference in body weight or body size between them and natural breeding goats. [ Condusioa] The study lays a foundation for further studies on the breedinq and utilization of Boer noats from embryo transplant at early staae.

Earthquake engineering in China

The development of earthquake engineering in China is described into three stages.The initial stage in 1950's- 1960's was marked with the initiation of this branch of science from its creation in the first national 12-year plan of science and technology by specifying earthquake engineering as a branch item and IEM was one participant.The first earthquake zonation map and the first seismic design code were soon completed and used in engineering design.Site effect on structural design and site selection were seriously studied.The second stage marked with the occurrence of quite a few strong earthquakes in China, from which many lessons were learned and corresponding considerations were specified in our design codes and followed in construction practice.The third stage is a stage of disaster management,which is marked by a series of government documentations,leading by a national law of the People's Republic of China on the protecting against and mitigating earthquake disasters adopted at the meeting of the Standing Committee of the National People's Congress of the People's Republic of China in 1997,and then followed by some provincial and municipal laws to force the actions outlined in the national law.It may be expected that our society will be much more safer to resist the attack of future strong earthquakes with less losses.Lastly, possible future developments are also discussed.

Development Stage and Evolution Cause of European Union Agriculture and Suggestions for Chinese Agricultural Development

Agriculture is a basic industry related to national economy and people’s livelihood,and is also the cornerstone of national economic development. European Union(EU) is the second largest exporter of agricultural products in the world,and its agricultural production change has international influence. In this paper,starting from two angles of EU agricultural development scale and target,development stage of its agricultural production is explored,and development and evolution causes of EU agriculture are analyzed: efficiency and benefit targets cause change of EU agricultural management model; change of agricultural product supply-demand pattern leads the evolution of EU agricultural development idea; the reflection of agricultural development target impels the transformation of EU agricultural development. On this basis,the below conclusions are obtained: agricultural development could evolve according to the changes of economic and social conditions; it is a necessary requirement of promoting internal market stability and international market harmony to actively use agricultural support policies adapting to agricultural development at each stage; it is an important measurement of impelling agricultural modernization process to promote innovation of agricultural management model and development of market integration; it is an inevitable road of realizing sustainable development of agriculture to develop ecological agriculture and emphasize equal development of agriculture and countryside. On this basis,it could provide the reference for agricultural development of China based on EU agricultural development experience.

Response of Soil Microbial Properties to Long-Term Application of Organic and Inorganic Amendments in a Tropical Soil (Saria, Burkina Faso)

Soil microbial biomass carbon (MBC), β-glucosidase, acid phosphatase and fluorescein diacetate (FDA) activities and bacterial community structure were assessed in a long-term (26 years) experiment, at physiological stages of sorghum growth, comparing different management methods for organic (manure, straw residues) and inorganic (urea) amendments at the INERA field station in Saria (Burkina Faso). Annual application of manure led to the highest soil microbial biomass and enzyme activities. Investigations indicated that only microbial biomass and β-glucosidase activities were affected during the cropping season. Phosphatase and FDA enzyme activities did not depend on the crop development stages. The application of N fertilizer modified phosphatase and FDA enzyme activities, the activities being higher in soils amended with N fertilizer. The bacterial community structure was analyzed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the eubacterial 16S rRNA gene. Cluster analysis of PCR-DGGE patterns showed two major clusters, the first containing the mineral fertilization and straw treatments and the second, the straw + urea, manure and manure + urea treatments. Sorghum grain yields were the highest for manure treatments. In this long-term experiment, applying straw did not produce a better grain yield than that obtained in the un-amended plot.

Spatial Heterogeneity and Influencing Factors of HFRS Epidemics in Rural and Urban Areas:A Study in Guanzhong Plain of Shaanxi Province,China

Objective The Guanzhong Plain of Shaanxi Province is a severely afflicted hemorrhagic fever with renal syndrome(HFRS)epidemic area,while HFRS prevalence has decreased in most epidemic areas in China.Little information is available regarding the leading fine-scale influencing factors in this highly HFRSconcentrated area and the roles of natural environmental and socioeconomic factors.To investigate this,two regions in the Guanzhong Plain,that is,the Chang’an District and Hu County,with similar geographical environments,different levels of economic development,and high epidemic prevalence,were chosen as representative areas of the HFRS epidemic.Methods Maximum entropy models were constructed based on HFRS cases and fine-scale influencing factors,including meteorological,natural environmental,and socioeconomic factors,from 2014 to 2016.Results More than 95% of the HFRS cases in the study area were located in the northern plains,which has an altitude of less than 800 m,with topography contributed 84.1% of the impact on the spatial differentiation of the HFRS epidemic.In the northern plains,precipitation and population density jointly affected the spatial differentiation of the HFRS epidemic,with contribution rates of 60.7% and 28.0%,respectively.By comparing the influencing factors of the northern plains of Chang’an District and Hu County,we found that precipitation and the normalized difference vegetation index(NDVI)dominated the HFRS epidemic in the relatively developed Chang’an District,while land-use type,temperature,precipitation and population density dominated the HFRS epidemic in the relatively undeveloped Hu County.Conclusion Topography was the primary key factor for HFRS prevalence in the Chang’an District and Hu County,and the spatial differentiation of HFRS was dominated by precipitation and population density in the northern plains.Compared with the influencing factors of the relatively developed Chang’an District,the developing Hu County was more affected by socioeconomic factors.When formulating targeted HFRS epidemic prevention and control strategies in the targeted areas,it is crucial to consider the local economic development state and combine natural environmental factors,including the meteorological environment and vegetation coverage.

Identification and Characterization of Gene SpDMRT99B and Its Sex-Biased Expression Profile in the Mud Crab,Scylla paramamosain

The doublesex and mab-3 related transcription factor(DMRT)gene family is conserved from invertebrates to humans.The functions of DMRT are mainly involved in sex development and the formation of many tissues and organs.In this study,a DM(Doublesex/Mab-3-domain gene was identified in the mud crab Scylla paramamosain,and was named SpDMRT99B because of its many similarities to arthropod DMRT99B and phylogenetically close relationship with arthropod DMRT99B.The cDNA of SpDMRT99B gene is 1249 bp in length,encoding 224 amino acids.From 254 bp to 928 bp there is a conserved DM domain.No transmembrane domain was identified.Through multiple amino acid alignment and phylogenetic tree analysis,the closest gene to SpDMRT99B is Eriocheir sinensis DMRT99B,followed by Macrobrachium rosenbergii and Drosophila melanogaster DMRT99B.The expressions of the gene were characterized in different tissues of female and male crabs during early development period of crab individuals,as well as in different development periods of gonads.The results showed that SpDMRT99B gene is significantly highly expressed in testis than in ovary and other tissues.The expression level of SpDMRT99B in testis at different stages is significantly higher than that in ovary,and it is particularly highly expressed in immature testis.In early developmental stages of larvae,the expressions of SpDMRT99B remain at a low level and reach a peak at zoea stage I when the body segments shape up.It is speculated that SpDMRT99B gene might be involved in the gonadal development process and somitogenesis of S.paramamosain.

Discussion on the Regional Division of the Development Stage of China's Wind Power

This paper constructs the index system of the regional division of the development stage of China's wind power resources,including the index of energy,the index of wind energy endowments and other indices.Based on principal component analysis and layered clustering analysis of these indices,and combined with the conceptual function of the development and utilization stage of the wind power,this paper divides the development and utilization stage of the wind power into four stages taking province as the basic yardstick:optimization growth stage,the rapid growth stage,the slow growth stage and the initial growth stage.In addition,this paper briefly discusses the basic strategy that should be adopted in each development stage of wind power resources.

A critical analysis of environmental evolutionin the loess-plateau area

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The ＂Middle Income Trap＂ in Economic Growth in Different Economies

Nowadays,more than 50%of the world population live in middle-income economies.Economies in the middle-income development stage are confronted with a number of challenges,such as economic restructuring,industrial upgrade and income growth.Therefore,academia around the world have paid much attention to theoretical and empirical researches of the "middle income trap " and long-term trends of global economic growth.Based on analyses about the long-term growth path of the world economy and the characteristics of different stages of economic development,this paper defines the "middle income trap" and its characteristics and examines,through a large number of cases from different economies,the reasons why "growth slowdown " arises during the middle-income development stage.These theoretic and empirical researches have reference value and heuristic meaning for China to actively respond to the challenges of the "middle income trap ".

Characteristics of China＇s Development in the New Era Explained in Light of Economic Principles

As China entered into a new era of development, the primary contradiction facing Chinese society has also evolved. It is important for both scholars and policymakers to review the characteristics of China's economy in its present stage in accordance with economic principles-principles that reflect every aspect of economic and social life and macroeconomic regulation. In this paper, we attempt to unravel the characteristics of China's new era from three perspectives-historical materialism, dialectic materialism and epistemology, and in light of economic principles at the three levels of resource factors, modes of production and regulation of resource allocation. Based on these perspectives, this paper further identifies the characteristics of China's new era from nine aspects, including globalization, modernization, the primary stage of socialism, new development concepts, supply-side reforms, the guideline of seeking steady progress, the mentality of scope, bottomline mindset and strategic composure. From the three different levels, this paper summarizes and applies economic principles to propose solutions to the problems and challenges facing China in the new era. The findings of the paper are also relevant to implementation of the guidelines CPC Central Committee General Secretary Xi Jinping noted in his report to the 19 th CPC National Congress.