The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants w...The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants were carried out by means of PCR,Southern blotting and field characterization. The results showed that the expression ofrol genes greatly increased the rooting ability of the transgenic plants, and changed theplant development. Highly male-sterile plants with strong apical dominance and fertileplants with short internodes, stunted growth and improved economic characteristics weresegregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by35S promoter. The transgenic lines of rol ABC construct usually had normal boll settingand slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.展开更多
The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnorm...The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found dudng double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X （33.00%）. Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.展开更多
基金the National Natural science Fundationof china(39970386)
文摘The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants were carried out by means of PCR,Southern blotting and field characterization. The results showed that the expression ofrol genes greatly increased the rooting ability of the transgenic plants, and changed theplant development. Highly male-sterile plants with strong apical dominance and fertileplants with short internodes, stunted growth and improved economic characteristics weresegregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by35S promoter. The transgenic lines of rol ABC construct usually had normal boll settingand slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.
基金supported by the National Science & Technology Pillar Program of China in the Tenth Five-Year Plan Period(Grant No.2001BA302B)the Education Department of Henan Province, China(Grant No.2008A208019)
文摘The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found dudng double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X （33.00%）. Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.
