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Electrochemical detection of human ferritin based on gold nanorod reporter probe and cotton thread immunoassay device 被引量:1
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作者 Ting-Ting Song Wei Wang +3 位作者 Li-Li Meng Yan Liu Xiao-Bo Jia Xun Mao 《Chinese Chemical Letters》 SCIE CAS CSCD 2017年第2期226-230,共5页
In this study,a natural cotton thread immunoassay device combined with gold nanorod(GNR) reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung ca... In this study,a natural cotton thread immunoassay device combined with gold nanorod(GNR) reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle(GNP) report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry(ASV) testing,which meet the requirement for clinical diagnosis. 展开更多
关键词 Cotton thread immunoassay device Gold nanorod reporter probe Point-of-care diagnosis Protein detection Electrochemical immunoassay
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