Transcriptome analysis of Idesia polycarpa Maxim. var vestita Diels fl owers during sex differentiation

Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.

On the Farmers' Role Differentiation and the Effective Implementation of Rural Vitalization Strategy——A Perspective from the Targets,Process and Evaluation of Policy Implementation

During the historical period of securing a decisive victory in building a moderately prosperous society in all respects and striving for the great success of socialism with Chinese characteristics for a new era, the 19 th National Congress of the CPC put forward the signi cant deployment of rural vitalization strategy, drawing a great blueprint for the prospect of China's "three issues" of agriculture, rural areas, and farmers and acting as an important focus of these "three issues" in the future. This paper discussed the relationships between farmers' role di erentiation and ve objectives of the rural vitalization strategy on the basis of farmers' role di erentiation and conversion in the process of agriculture and countryside modernization and the interpretation of farmers' role di erentiation and the rural vitalization strategy. Based on the logical relationships between farmers' role di erentiation and the rural vitalization strategy, we discuss the e ective implementation of the rural vitalization strategy in terms of its targets, processes and e ectiveness evaluations. We also propose that the implementation of the rural vitalization strategy can only be implemented when farmers are given the rst priority as strategy subjects, farmers' wills are respected in the implementation process, and farmers' satisfaction is highlighted in the strategy implementation e ectiveness evaluations.

Building an international platform for the research,development,and dissemination of new knowledge in the field of laboratory animal science,technology,and experimental medicine

Dear Colleagues,It is my distinct honor to serve as the Honorary Chief Editor of AMEM,an international open-access journal,dedicated to serve as a platform for international exchange,and the translation of research outcomes in the field of life sciences.AMEM strives to establish itself as a high-level international academic exchange platform that brings together scientific wisdom from around the world and offers a pres-tigious journal for presenting research findings,and disseminating state of the art knowledge in the vibrant fields of life sciences.

DINP加氢制备环保型增塑剂DINCH的研究进展

邻苯二甲酸酯(PAEs)是聚合物产品中使用最广泛的增塑剂之一,和人们的生活息息相关。但由于其对环境以及人类健康的不利影响普遍存在,具有潜在的生殖毒性、内分泌干扰和致癌性,一些产品已被限制或淘汰,从而引起了新型环保增塑剂的开发热潮,其中环己烷-1,2-二羧酸酯类增塑剂因性能优异、无毒无害而受到广泛关注,被认为是邻苯二甲酸酯类增塑剂的更安全替代品。本文综述了环己烷-1,2-二羧酸酯中环己烷-1,2-二羧酸二异壬酯(DINCH)的合成方法,重点介绍了负载型单金属催化剂、负载型双金属催化剂、负载型多金属催化剂在催化加氢合成环保型增塑剂DINCH中的研究进展,为催化剂的研究方向提出了建议。

罕见抗-Di^(b)致严重胎儿新生儿溶血病的实验室检测与相关研究

目的对1例高频抗体导致的胎儿新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)进行检测、鉴定及配血。方法对患儿进行新生儿溶血试验,对母亲进行血清学意外抗体鉴定,并对母亲红细胞进行常见高频抗原鉴定;对检出抗体进行IgG分型检测,并用流式细胞术进行单核细胞体外吞噬致敏红细胞试验,以检测抗体相关的吞噬率;对患儿母亲、父亲及舅舅进行相关红细胞血型基因测序;利用稀释的母亲血浆和抗人球卡法,在献血者中进行大规模相合血液的筛选。结果产妇鉴定为Di(b-)稀有血型,产生了抗-Di b(效价512)并导致了严重的HDFN;抗-Di b亚型分型为IgG1和IgG2型,单核细胞体外吞噬效率为88.83%(74.7/84.09);产妇亲属中没有相合献血者,后续从5520名献血者中筛选到2例Di(b-)相合血液,患儿接收输血治疗后康复出院。后续在51334名献血者中筛查到17名Di(b-)献血者,该数据表明Di(b-)在广州地区献血者中的分布频率约为三千分之一(0.033%,17/51334)。结论综合利用血型血清学及分子生物学方法诊断了抗-Di b所致的严重HDFN,建立了1种有效大规模筛查Di(b-)稀有血型的方法并找到相合血液,为建立Di(b-)稀有血型库奠定了基础。

A Novel Integrated Stability Control Based on Differential Braking and Active Steering for Four-axle Trucks

Di erential braking and active steering have already been integrated to overcome their shortcomings. However, existing research mainly focuses on two-axle vehicles and controllers are mostly designed to use one control method to improve the other. Moreover, many experiments are needed to improve the robustness; therefore, these control methods are underutilized. This paper proposes an integrated control system specially designed for multi-axle vehicles, in which the desired lateral force and yaw moment of vehicles are determined by the sliding mode control algorithm. The output of the sliding mode control is distributed to the suitable wheels based on the abilities and potentials of the two control methods. Moreover, in this method, fewer experiments are needed, and the robustness and simultaneity are both guaranteed. To simplify the optimization system and to improve the computation speed, seven simple optimization subsystems are designed for the determination of control outputs on each wheel. The simulation results show that the proposed controller obviously enhances the stability of multi-axle trucks. The system improves 68% of the safe velocity, and its performance is much better than both di erential braking and active steering. This research proposes an integrated control system that can simultaneously invoke di erential braking and active steering of multi-axle vehicles to fully utilize the abilities and potentials of the two control methods.

4DI110型空压机的技术改造与操作优化

文章通过介绍新引进的4DI110型离心式空压机(以下简称“空压机”)试运行阶段开始以来的运行情况和气温变化对空压机出口流量造成的影响,分析空压机出口流量随气温变化而变化的规律,指出空压机防喘阀V3004阀不能自动调节出口压力,影响空分装置工况的稳定。为实现空压机出口压力的稳定,对其防喘阀V3004阀采取增设自动旁路阀V3008阀的技术改造,同时优化和完善相关逻辑控制并阐述了空压机经技术改造后的操作优化和取得的实际效果。

3 di-sk树的计数

3 di-sk树是一种不存在标记为(2,2)左边的混合三元树.研究3 di-sk树上的几类计数问题,并给出了有n个内点的3 di-sk树的集合与从(0,0)到(3 n,0)的3-Schroder路的集合之间的双射.

Cooperative Differential Game for Model Energy-Bandwidth Efficiency Tradeoff in the Internet of Things

Internet of Things （IoT） refers to an infrastructure which enables the forms of com- munication and collaboration between people and things, and between things themselves. In order to improve its performance, we present a tradeoff between bandwidth and energy con- sumption in the loT in this paper. A service providing model is built to find the relation- ship between bandwidth and energy consump- tion using a cooperative differential game mo- del. The game solution is gotten in the condi- tion of grand coalition, feedback Nash equili- brium and intermediate coalitions and an allo- cation policy is obtain by Shapley theory. The results are shown as follows. Firstly, the per- formance of IoT decreases with the increasing of bandwidth cost or with the decreasing of en- ergy cost; secondly, all the nodes in the IoT com- posing a grand coalition can save bandwidth and energy consumption; thirdly, when the fac- tors of bandwidth cost and energy cost are eq- ual, the obtained number of provided services is an optimised value which is the trade-off between energy and bandwidth consumption.

On Relationship between Lower-order of Coefficients and Growth of Solutions of Complex Differential Equations near a Singular Point

We investigate the growth of solutions of the following complex linear di er-ential equation f″+A(z)f′+B(z)f=0,where A(z)and B(z)are analytic functions in -C-{z0},z0∈C.Some estimations of lower bounded of growth of solutions of the di erential equation are obtained by using the concept of lower order.

A STUDY ON' SYNCHRONOUS-HI GH-DERIVATIVE SPECTFLUOR—THE DI FFERENTI ATI ON OF CRUDE OIL AND FUEL OIL POLLUTION

A method of synchronous-high-derivative spectfluor for identification of crude oil and fuel oil pollution is studied. The best operation conditions for the 2nd and 4th deriv, are set. To differentiate oil-spill at river and sea, this method is rapid and simple, and the spectra have high resolution power as 'fingerprint'.

借助DIS实验突破教学难点——以“磁场对通电导线的作用力”为例

教材中直接得出安培力的公式,该知识点难度较大,传统实验现象不够明显且只能定性探究,学生无法真正理解该物理规律和公式。传统实验加DIS实验可对物理规律进行定性和定量的探究,有利于学生物理学科核心素养的培养。

用DIS电磁定位魔板探究平抛运动的教学设计

随着数字化信息系统在中学物理教学中的逐步融入,我国中学物理实验呈现智能化、现代化的发展趋势.以DIS电磁定位魔板为实验装置,通过科学探究的关键环节,设计探究平抛运动的教学流程.实践发现传感器实验也适合做科学探究,能够充分提升学生科学探究和推理论证的能力.

城市轨道交通减振轨道DI型弹条断裂原因分析及治理效果

[目的]弹条是减振轨道扣件中的关键部件,其力学性能及承受疲劳载荷性能关系到城市轨道交通列车运行安全及轨道使用寿命。针对目前弹条失效破坏频发的情况,有必要对其断裂原因进行分析并制定合理治理措施。[方法]以某城市轨道交通线路某区段出现弹条集中断裂的情况为依托,通过弹条断口分析确定弹条的断裂类型为疲劳断裂,通过分析弹条的化学成分和物理性能未发现异常状态,通过仿真分析和试验分析得到弹条的固有频率。结合弹条断裂区段轨道动态测试得到轨道和弹条的振动特性,由此确定轨道波磨可能是引起弹条出现反复断裂的主要原因。针对该原因制定了合理的治理措施,对治理前后的弹条断裂数量进行了统计。[结果及结论]钢轨打磨措施有效抑制了轨道波磨,大大减少了弹条断裂情况的发生,进一步佐证了轨道波磨是引起弹条断裂的主要原因。

嗜热链球菌c-di-AMP合成酶的结构预测

作为一种新兴第二信使分子,c-di-AMP具有全局调控胞内渗透压平衡、DNA损伤和生物膜形成等生理功能。本实验室在实验前期筛选出了一株高产胞外多糖的嗜热链球菌菌株S-3,但未对其进行c-di-AMP信号通路研究。本研究在嗜热链球菌S-3全基因组中筛选出c-di-AMP合成酶(StDAC),对其二级结构进行预测,结果显示St DAC二级结构包含42.98%α-螺旋、15.32%β-折叠和38.30%无规则卷曲。利用SWISS-MODEL和Modeller对StDAC进行单模板和多模板同源建模,由SWISS-MODEL建立的模型通过了Ramachandran、Errat和Verify-3D评估,表明其结构较为合理;而Modeller建立的模型仅通过了Ramachandran评估,总体结构评分较低。本研究预测的嗜热链球菌StDAC三维模型为解析其结构和功能奠定了基础。

乳酸菌第二信使分子c-di-AMP研究进展

微生物依赖信号识别和信号传递来感知并响应外界环境变化。c-di-AMP作为第二信使分子在信号传导过程中发挥着关键作用,其通过酶、转录调控因子和核糖开关等受体靶标来调控细胞生长、生物膜形成、K^(+)稳态、DNA完整性、细胞壁合成和脂肪酸合成等与生理功能相关的基因和蛋白的表达。尽管对于c-di-AMP代谢调控在枯草芽孢杆菌、单增李斯特氏菌和金黄色葡萄球菌等细菌中的研究较为深入,但对其在乳酸菌中的研究却相对较少。基于此,文章重点关注乳酸菌中cdi-AMP的代谢及其在K^(+)稳态和抑制甘氨酸甜菜碱转运中的信号传导作用;同时,通过总结现有研究中的乳酸菌c-di-AMP代谢调控,深化对乳酸菌第二信使分子信号调控的认知。

Semantic Differential as an Assessment Tool of （Dis） Advantages of QMS in the Light of Accredited Certification in Poland

This article is a continuation of the issue in the field of assessment of （dis）advantages resulting from the implementation of the quality management systems （QMS） in a structure of the organization. The publication uses the results of studies conducted in the range of assessment＇s information completeness provided by the certification bodies （CBs） in the context of advantages and disadvantages of the QMS implementation in accordance with PN-EN ISO 9001. In addition, an attempt to define a tool for the QMS assessment of （dis）advantages in the organization has been made. The basis for empirical analysis of the issue was the information provided by an accredited management systems CBs recommended by the Polish Centre for Accreditation （PCA）. There are 33 units in Poland, which were granted by PCA with the accreditation within the range of ISO 9001：2008. In the first stage of the research work, a substantive analysis of websites content among mentioned CBs has been carried out. In addition, the paper proposes use of semantic differential method （SD） to evaluate （dis）advantages of the QMS. SD adaptation for the assessment of the QMS （dis）advantages is the authorial concept. The authors hope for polemic in this area. In the face of obtained results, an authorial SD construction has been proposed, which is useful and may help in the formulation and conveying of the relevant （reliable） information to potential clients in the range of （dis）advantages resulting from the QMS implementation to the organization. As a consequence, the recommendations have been set, which are kind of guidelines that could significantly influence the realignment of the information state conveyed on the internet by the CBs.

外卖包装材料对我国青年群体暴露DBP和DiBP的影响

青年群体是外卖食品最大的消费群体,外卖包装材料中的污染物对青年群体的危害不容忽视。文章分析了外卖包装材料对我国青年群体暴露邻苯二甲酸二正丁酯(DBP)和邻苯二甲酸二异丁酯(DiBP)的影响,结果表明,发泡聚苯乙烯(PS)餐盒中DBP和DiBP的浓度(2986.27 ng/g和1345.20 ng/g)显著高于聚丙烯(PP)餐盒、纸质餐盒和锡纸餐盒,青年人群使用PS餐盒暴露DBP和DiBP的暴露量约为使用纸质餐盒和锡纸餐盒的2~5倍,青年人群经外卖餐盒暴露DBP和DiBP的暴露量占经饮食途径暴露DBP和DiBP暴露总量的1.72%~9.49%,占体内DBP和DiBP暴露总量的0.09%~0.47%。外卖包装材料是青年人群暴露DBP和DiBP的重要来源之一。

DIS实验助力高中物理教学探究——以图象问题为例

实验探究在高中物理教学中扮演着十分重要的角色。在数字化信息系统(DIS)实验的帮助下,弥补了一些传统实验在定量探究上的局限,极大地提升了实验教学效率。这种创新的教学方式有助于培养学生的实验探究精神,让他们养成严谨对待实验结果并深入剖析数据的科学习惯。本文立足于教学实践,详细探讨了如何有效利用DIS实验助力高中物理教学探究。以"用DIS实验探索力的相互作用、探究向心力实验、验证动量定理的实验案例"为例,展示了DIS实验在课堂中的精准、直观以及实际操作价值。

基于核心素养的高中物理DIS实验 校本课程开发的实践研究 ——以“探究平抛运动的特点”为例

运用传感器和计算机技术的DIS实验,兼具精确测量数据和快速直观展示结果的特点,在教育信息化的大背景下,逐渐在高中物理教学中普及。文章以探究平抛运动的特点为例,借助DIS实验的优点进行校本课程的开发,实现对学生核心素养的补充培养。通过教学过程中的实践和反思提出了基于学科核心素养应用DIS实验开发校本课程应注意的一些问题。