目的对1例高频抗体导致的胎儿新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)进行检测、鉴定及配血。方法对患儿进行新生儿溶血试验,对母亲进行血清学意外抗体鉴定,并对母亲红细胞进行常见高频抗原鉴定;对检出抗体进行...目的对1例高频抗体导致的胎儿新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)进行检测、鉴定及配血。方法对患儿进行新生儿溶血试验,对母亲进行血清学意外抗体鉴定,并对母亲红细胞进行常见高频抗原鉴定;对检出抗体进行IgG分型检测,并用流式细胞术进行单核细胞体外吞噬致敏红细胞试验,以检测抗体相关的吞噬率;对患儿母亲、父亲及舅舅进行相关红细胞血型基因测序;利用稀释的母亲血浆和抗人球卡法,在献血者中进行大规模相合血液的筛选。结果产妇鉴定为Di(b-)稀有血型,产生了抗-Di b(效价512)并导致了严重的HDFN;抗-Di b亚型分型为IgG1和IgG2型,单核细胞体外吞噬效率为88.83%(74.7/84.09);产妇亲属中没有相合献血者,后续从5520名献血者中筛选到2例Di(b-)相合血液,患儿接收输血治疗后康复出院。后续在51334名献血者中筛查到17名Di(b-)献血者,该数据表明Di(b-)在广州地区献血者中的分布频率约为三千分之一(0.033%,17/51334)。结论综合利用血型血清学及分子生物学方法诊断了抗-Di b所致的严重HDFN,建立了1种有效大规模筛查Di(b-)稀有血型的方法并找到相合血液,为建立Di(b-)稀有血型库奠定了基础。展开更多
Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mec...Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.展开更多
During the historical period of securing a decisive victory in building a moderately prosperous society in all respects and striving for the great success of socialism with Chinese characteristics for a new era, the 1...During the historical period of securing a decisive victory in building a moderately prosperous society in all respects and striving for the great success of socialism with Chinese characteristics for a new era, the 19 th National Congress of the CPC put forward the signi cant deployment of rural vitalization strategy, drawing a great blueprint for the prospect of China's "three issues" of agriculture, rural areas, and farmers and acting as an important focus of these "three issues" in the future. This paper discussed the relationships between farmers' role di erentiation and ve objectives of the rural vitalization strategy on the basis of farmers' role di erentiation and conversion in the process of agriculture and countryside modernization and the interpretation of farmers' role di erentiation and the rural vitalization strategy. Based on the logical relationships between farmers' role di erentiation and the rural vitalization strategy, we discuss the e ective implementation of the rural vitalization strategy in terms of its targets, processes and e ectiveness evaluations. We also propose that the implementation of the rural vitalization strategy can only be implemented when farmers are given the rst priority as strategy subjects, farmers' wills are respected in the implementation process, and farmers' satisfaction is highlighted in the strategy implementation e ectiveness evaluations.展开更多
A dispersion interferometer(DI)has been installed and operates on the Experimental Advanced Superconducting Tokamak(EAST).This DI system utilizes a continuous-wave 9.3μm CO_(2)laser source to measure line-averaged el...A dispersion interferometer(DI)has been installed and operates on the Experimental Advanced Superconducting Tokamak(EAST).This DI system utilizes a continuous-wave 9.3μm CO_(2)laser source to measure line-averaged electron densities accurately.In contrast to conventional interferometers,the DI does not require substantial vibration isolations or compensating systems to reduce the impact of vibrations in the optical path.It also employs a ratio of modulation amplitudes,ensuring it remains immune to the variations in detected intensities.Without a variation compensation system,the DI system on EAST reaches a density resolution of less than1.8×10^(-2)πrad and a temporal resolution of 20μs.The measurements made by the POlarimeterINTerferometer(POINT)system and the far-infrared hydrogen cyanide(HCN)interferometer are remarkably consistent with the DI’s results.The possibility of fringe jumps and the impact of refraction in high-density discharge can be significantly decreased using a shorter wavelength laser source.A rapid density change of 3×10^(19)m^(-3)during 0.15 s has been measured accurately in shot No.114755 of EAST.Additionally,the DI system demonstrates dependability and stability under 305 s long-pulse discharges in shot No.122054.展开更多
Huang Di Nei Jing(《黄帝内经》The Yellow Emperor’s Inner Classic) has been the source text of Chinese medicine knowledge and innovation for over two thousand years. Despite this key relevance, many of its ideas and p...Huang Di Nei Jing(《黄帝内经》The Yellow Emperor’s Inner Classic) has been the source text of Chinese medicine knowledge and innovation for over two thousand years. Despite this key relevance, many of its ideas and practices have proven difficult to understand and implement fully into clinical practice. Cultural and language differences can be compounded with these challenges but may also present new opportunities for advancement and insight when studied by researchers outside of the originating culture. This article introduces the method of Classical-Text Archaeology and delves into the author’s two-decade journey of researching this text, with a discussion on cultural differences and issues of medical scholarship.展开更多
Purpose:This study investigated the publication behaviour of 573 chief editors managing 432 Social Sciences journals in Turkey.Direct inquiries into editorial qualifications are rare,and this research aims to shed lig...Purpose:This study investigated the publication behaviour of 573 chief editors managing 432 Social Sciences journals in Turkey.Direct inquiries into editorial qualifications are rare,and this research aims to shed light on editors’scientific leadership capabilities.Design/methodology/approach:This study contrasts insider publication behaviour in national journals with international articles in journals indexed by the Web of Science(WOS)and Scopus.It argues that editors demonstrating a consistent ability to publish in competitive WOS and Scopus indexed journals signal high qualifications,while editors with persistent insider behaviour and strong local orientation signal low qualification.Scientific leadership capability is measured by first-authored publications.Correlation and various regression tests are conducted to identify significant determinants of publication behaviour.Findings:International publications are rare and concentrated on a few individuals,while insider publications are endemic and constitute nearly 40%of all national articles.Editors publish 3.2 insider papers and 8.1 national papers for every SSCI article.62%(58%)of the editors have no SSCI(Scopus)article,53%(63%)do not have a single lead-authored WOS(Scopus)article,and 89%publish at least one insider paper.Only a minority consistently publish in international journals;a fifth of the editors have three or more SSCI publications,and a quarter have three or more Scopus articles.Editors with foreign Ph.D.degrees are the most qualified and internationally oriented,whereas non-mobile editors are the most underqualified and underperform other editors by every measure.Illustrating the overall lack of qualification,nearly half of the professor editors and the majority of the WOS and Scopus indexed journal editors have no record of SSCI or Scopus publications.Research limitations:This research relies on local settings that encourage national publications at the expense of international journals.Findings should be evaluated in light of this setting and bearing in mind that narrow localities are more prone to peer favouritism.Practical implications:Incompetent and nepotistic editors pose an imminent threat to Turkish national literature.A lasting solution would likely include the dismissal and replacement of unqualified editors,as well as delisting and closure of dozens of journals that operate in questionable ways and serve little scientific purpose.Originality/value:To my knowledge,this is the first study to document the publication behaviour of national journal chief editors.展开更多
文摘目的对1例高频抗体导致的胎儿新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)进行检测、鉴定及配血。方法对患儿进行新生儿溶血试验,对母亲进行血清学意外抗体鉴定,并对母亲红细胞进行常见高频抗原鉴定;对检出抗体进行IgG分型检测,并用流式细胞术进行单核细胞体外吞噬致敏红细胞试验,以检测抗体相关的吞噬率;对患儿母亲、父亲及舅舅进行相关红细胞血型基因测序;利用稀释的母亲血浆和抗人球卡法,在献血者中进行大规模相合血液的筛选。结果产妇鉴定为Di(b-)稀有血型,产生了抗-Di b(效价512)并导致了严重的HDFN;抗-Di b亚型分型为IgG1和IgG2型,单核细胞体外吞噬效率为88.83%(74.7/84.09);产妇亲属中没有相合献血者,后续从5520名献血者中筛选到2例Di(b-)相合血液,患儿接收输血治疗后康复出院。后续在51334名献血者中筛查到17名Di(b-)献血者,该数据表明Di(b-)在广州地区献血者中的分布频率约为三千分之一(0.033%,17/51334)。结论综合利用血型血清学及分子生物学方法诊断了抗-Di b所致的严重HDFN,建立了1种有效大规模筛查Di(b-)稀有血型的方法并找到相合血液,为建立Di(b-)稀有血型库奠定了基础。
文摘Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.
基金“Study on Farmers’Participation in the Management of Field Irrigation System:Resource Endowment,Organization Support,and Governance Performance,”National Natural Science Foundation of China Project(Code:71773092)“Studies on the Weakness of Poverty of Rural Residents in Closely Grouped Areas,”Social Science Foundation of Ministry of Education Project(Code:17YJA790102)
文摘During the historical period of securing a decisive victory in building a moderately prosperous society in all respects and striving for the great success of socialism with Chinese characteristics for a new era, the 19 th National Congress of the CPC put forward the signi cant deployment of rural vitalization strategy, drawing a great blueprint for the prospect of China's "three issues" of agriculture, rural areas, and farmers and acting as an important focus of these "three issues" in the future. This paper discussed the relationships between farmers' role di erentiation and ve objectives of the rural vitalization strategy on the basis of farmers' role di erentiation and conversion in the process of agriculture and countryside modernization and the interpretation of farmers' role di erentiation and the rural vitalization strategy. Based on the logical relationships between farmers' role di erentiation and the rural vitalization strategy, we discuss the e ective implementation of the rural vitalization strategy in terms of its targets, processes and e ectiveness evaluations. We also propose that the implementation of the rural vitalization strategy can only be implemented when farmers are given the rst priority as strategy subjects, farmers' wills are respected in the implementation process, and farmers' satisfaction is highlighted in the strategy implementation e ectiveness evaluations.
基金supported by the Comprehensive Research Facility for Fusion Technology Program of China(No.2018-000052-7301-001228)the Major Special Science and Technology Project of Anhui Province(No.912188707023)。
文摘A dispersion interferometer(DI)has been installed and operates on the Experimental Advanced Superconducting Tokamak(EAST).This DI system utilizes a continuous-wave 9.3μm CO_(2)laser source to measure line-averaged electron densities accurately.In contrast to conventional interferometers,the DI does not require substantial vibration isolations or compensating systems to reduce the impact of vibrations in the optical path.It also employs a ratio of modulation amplitudes,ensuring it remains immune to the variations in detected intensities.Without a variation compensation system,the DI system on EAST reaches a density resolution of less than1.8×10^(-2)πrad and a temporal resolution of 20μs.The measurements made by the POlarimeterINTerferometer(POINT)system and the far-infrared hydrogen cyanide(HCN)interferometer are remarkably consistent with the DI’s results.The possibility of fringe jumps and the impact of refraction in high-density discharge can be significantly decreased using a shorter wavelength laser source.A rapid density change of 3×10^(19)m^(-3)during 0.15 s has been measured accurately in shot No.114755 of EAST.Additionally,the DI system demonstrates dependability and stability under 305 s long-pulse discharges in shot No.122054.
文摘Huang Di Nei Jing(《黄帝内经》The Yellow Emperor’s Inner Classic) has been the source text of Chinese medicine knowledge and innovation for over two thousand years. Despite this key relevance, many of its ideas and practices have proven difficult to understand and implement fully into clinical practice. Cultural and language differences can be compounded with these challenges but may also present new opportunities for advancement and insight when studied by researchers outside of the originating culture. This article introduces the method of Classical-Text Archaeology and delves into the author’s two-decade journey of researching this text, with a discussion on cultural differences and issues of medical scholarship.
文摘Purpose:This study investigated the publication behaviour of 573 chief editors managing 432 Social Sciences journals in Turkey.Direct inquiries into editorial qualifications are rare,and this research aims to shed light on editors’scientific leadership capabilities.Design/methodology/approach:This study contrasts insider publication behaviour in national journals with international articles in journals indexed by the Web of Science(WOS)and Scopus.It argues that editors demonstrating a consistent ability to publish in competitive WOS and Scopus indexed journals signal high qualifications,while editors with persistent insider behaviour and strong local orientation signal low qualification.Scientific leadership capability is measured by first-authored publications.Correlation and various regression tests are conducted to identify significant determinants of publication behaviour.Findings:International publications are rare and concentrated on a few individuals,while insider publications are endemic and constitute nearly 40%of all national articles.Editors publish 3.2 insider papers and 8.1 national papers for every SSCI article.62%(58%)of the editors have no SSCI(Scopus)article,53%(63%)do not have a single lead-authored WOS(Scopus)article,and 89%publish at least one insider paper.Only a minority consistently publish in international journals;a fifth of the editors have three or more SSCI publications,and a quarter have three or more Scopus articles.Editors with foreign Ph.D.degrees are the most qualified and internationally oriented,whereas non-mobile editors are the most underqualified and underperform other editors by every measure.Illustrating the overall lack of qualification,nearly half of the professor editors and the majority of the WOS and Scopus indexed journal editors have no record of SSCI or Scopus publications.Research limitations:This research relies on local settings that encourage national publications at the expense of international journals.Findings should be evaluated in light of this setting and bearing in mind that narrow localities are more prone to peer favouritism.Practical implications:Incompetent and nepotistic editors pose an imminent threat to Turkish national literature.A lasting solution would likely include the dismissal and replacement of unqualified editors,as well as delisting and closure of dozens of journals that operate in questionable ways and serve little scientific purpose.Originality/value:To my knowledge,this is the first study to document the publication behaviour of national journal chief editors.