Objective: To explore the ameliorative effect and mechanism of Panax notoginseng (PNG) and Ticlid in treating early diabetic nephropathy (DN).Method: Fifty-eight patients were divided randomly into two groups, 28 pati...Objective: To explore the ameliorative effect and mechanism of Panax notoginseng (PNG) and Ticlid in treating early diabetic nephropathy (DN).Method: Fifty-eight patients were divided randomly into two groups, 28 patients of the Ticlid group treated with Ticlid 250 mg orally, once a day and 30 patients of the PNG group treated with PNG 8 ml in 250 ml of normal saline intravenous drip once a day. The therapeutic effect and relative indexes of the two groups were observed and compared.Results: After treatment, in both groups, the thromboxane B2 markedly reduced and was more prominent in the Ticlid group (P<0.05), while the 6-keto-prostaglandin F1α increased obviously, so as to cause a significant lowering of T/K ratio, P<0. 01. Levels of urinary albumin, β2 microglobulin and blood ±1 microglobulin of both groups were lowered significantly, P<0. 01. A significant positive linear correlation was found in the Ticlid group between urinary albumin and T/K ratio (r = 0.41, P < 0.01), as well as in blood ±1 microglobulin with T/K ratio (r = 0. 34, P < 0.05), while it was not found in the PNG group.Conclusion: Ticlid and PNG were beneficial to resume the balance of T/K ratio and improve microcirculation, reduce whole blood viscosity and decrease urinary albumin so as to retard the progress of DN.展开更多
Objective： To explore the mechanism of the protective effects of Panax notoginseng saponins（PNS） on kidney in diabetic rats. Methods： Diabetic rat model was obtained by intravenous injection of alloxan, and the ra...Objective： To explore the mechanism of the protective effects of Panax notoginseng saponins（PNS） on kidney in diabetic rats. Methods： Diabetic rat model was obtained by intravenous injection of alloxan, and the rats were divided into model, PNS-100 mg/（kg·day） and PNS-200 mg/（kg·day） groups, 10 each. Another 10 rats injected with saline were served as control. Periodic acid-Schiff staining and immunological histological chemistry were used to observe histomorphology and tissue expression of bone morphogenetic protein-7（BMP-7）. Silent information regulator 1（SIRT1） was silenced in rat mesangial cells by RNA interference. The mR NA expressions of SIRT-1, monocyte chemoattractant protein-1（MCP-1）, transforming growth factor β1（TGF-β1） and plasminogen activator inhibitor-1（PAI-1） were analyzed by reverse transcription polymerase chain reaction. The protein expressions of SIRT1 and the acetylation of nuclear factor κB（NF-κB） P65 were determined by western blotting. The concentration of MCP-1, TGF-β1 and malondialdehyde（MDA） in culture supernatant were detected by enzyme-linked immuno sorbent assay. The activity of superoxide dismutase（SOD） was detected by the classical method of nitrogen and blue four. Results： In diabetic model rats, PNS could not only reduce blood glucose and lipid（P〈0.01）, but also increase protein level of BMP-7 and inhibit PAI-1 expression for suppressing fibrosis of the kidney. In rat mesangial cells, PNS could up-regulate the expression of SIRT1（P〈0.01） and in turn suppress the transcription of TGF-β1（P〈0.05） and MCP-1（P〈0.05）. PNS could also reverse the increased acetylation of NF-κB p65 by high glucose. In addition, redox regulation factor MDA was down-regulated（P〈0.05） and SOD was up-regulated（P〈0.01）, which were both induced by SIRT1 up-regulation. Conclusions： PNS could protect kidney from diabetes with the possible mechanism of up-regulating SIRT1, therefore inhibiting inflammation through decreasing the induction of inflammatory cytokines and TGF-β1, as well as activating antioxidant proteins.展开更多
文摘Objective: To explore the ameliorative effect and mechanism of Panax notoginseng (PNG) and Ticlid in treating early diabetic nephropathy (DN).Method: Fifty-eight patients were divided randomly into two groups, 28 patients of the Ticlid group treated with Ticlid 250 mg orally, once a day and 30 patients of the PNG group treated with PNG 8 ml in 250 ml of normal saline intravenous drip once a day. The therapeutic effect and relative indexes of the two groups were observed and compared.Results: After treatment, in both groups, the thromboxane B2 markedly reduced and was more prominent in the Ticlid group (P<0.05), while the 6-keto-prostaglandin F1α increased obviously, so as to cause a significant lowering of T/K ratio, P<0. 01. Levels of urinary albumin, β2 microglobulin and blood ±1 microglobulin of both groups were lowered significantly, P<0. 01. A significant positive linear correlation was found in the Ticlid group between urinary albumin and T/K ratio (r = 0.41, P < 0.01), as well as in blood ±1 microglobulin with T/K ratio (r = 0. 34, P < 0.05), while it was not found in the PNG group.Conclusion: Ticlid and PNG were beneficial to resume the balance of T/K ratio and improve microcirculation, reduce whole blood viscosity and decrease urinary albumin so as to retard the progress of DN.
基金Supported by National Natural Science Foundation of China(No.81273615)Zhejiang Provincial Natural Science Fund(No.Y2110849)
文摘Objective： To explore the mechanism of the protective effects of Panax notoginseng saponins（PNS） on kidney in diabetic rats. Methods： Diabetic rat model was obtained by intravenous injection of alloxan, and the rats were divided into model, PNS-100 mg/（kg·day） and PNS-200 mg/（kg·day） groups, 10 each. Another 10 rats injected with saline were served as control. Periodic acid-Schiff staining and immunological histological chemistry were used to observe histomorphology and tissue expression of bone morphogenetic protein-7（BMP-7）. Silent information regulator 1（SIRT1） was silenced in rat mesangial cells by RNA interference. The mR NA expressions of SIRT-1, monocyte chemoattractant protein-1（MCP-1）, transforming growth factor β1（TGF-β1） and plasminogen activator inhibitor-1（PAI-1） were analyzed by reverse transcription polymerase chain reaction. The protein expressions of SIRT1 and the acetylation of nuclear factor κB（NF-κB） P65 were determined by western blotting. The concentration of MCP-1, TGF-β1 and malondialdehyde（MDA） in culture supernatant were detected by enzyme-linked immuno sorbent assay. The activity of superoxide dismutase（SOD） was detected by the classical method of nitrogen and blue four. Results： In diabetic model rats, PNS could not only reduce blood glucose and lipid（P〈0.01）, but also increase protein level of BMP-7 and inhibit PAI-1 expression for suppressing fibrosis of the kidney. In rat mesangial cells, PNS could up-regulate the expression of SIRT1（P〈0.01） and in turn suppress the transcription of TGF-β1（P〈0.05） and MCP-1（P〈0.05）. PNS could also reverse the increased acetylation of NF-κB p65 by high glucose. In addition, redox regulation factor MDA was down-regulated（P〈0.05） and SOD was up-regulated（P〈0.01）, which were both induced by SIRT1 up-regulation. Conclusions： PNS could protect kidney from diabetes with the possible mechanism of up-regulating SIRT1, therefore inhibiting inflammation through decreasing the induction of inflammatory cytokines and TGF-β1, as well as activating antioxidant proteins.
基金National Major Scientific and Technological Special Project for Signaficant New Drugs Development(No.2011ZX09101-004-04)Innovation Team Project of Beijing University of Chinese Medicine(No.2011CXTD-19)+1 种基金Ministry of Education Key Laboratory of Health Preservation of Chinese MedicineBeijing Key Laboratory of Health Preservation of Chinese Medicine~~
