[Objectives]This study aimed to screen the suitable medium and symbiotic fungi for tissue culture of Paphiopedilum dianthum and to establish a rapid propagation system of Paphiopedilum,so as to promote the development...[Objectives]This study aimed to screen the suitable medium and symbiotic fungi for tissue culture of Paphiopedilum dianthum and to establish a rapid propagation system of Paphiopedilum,so as to promote the development of planting industry and commercial application of Paphiopedilum.[Methods]Using P.dianthum as an experimental materials,the optimal culture medium for P.dianthum was selected from DE medium,Harvais modified medium and(bark+DE)liquid medium.Then,six fungal species isolated from the original habitat soil of Paphiopedilum were co-cultured with P.dianthum on the selected optimal culture medium.By comparing the morphology,the growth amount and the physiological indexes,such as chlorophyll content,soluble sugar content and root vitality of tissue culture plantlets of P.dianthum co-cultured with the six fungal species,the suitable fungal strains for symbiosis with P.dianthum were screened out.[Results]In three kinds of culture media,P.dianthum grew normally.The growth rate was higher than that in the mediums containing DE,and there were no significant differences in the content of soluble sugar from the leaves of tissue culture plantlets,which were symbiotic with the six different fungi species.Among the six fungi species growing on the optimal medium,the growing rate of XH-A was the fastest,and that of YY001 was the slowest.The growth and physiological indexes of P.dianthum co-cultured with DY1 in DE medium were higher than those in the control group.[Conclusions]DE medium was the optimal tissue culture medium for P.dianthum,and the optimal symbiotic fungus was DY1.展开更多
基金Project of Construction of Advanced Horticulture Discipline Under Beijing Municipality(2020)Key Laboratory of Biological and Genetic Improvement of Horticultural Crops under Ministry of Agriculture。
文摘[Objectives]This study aimed to screen the suitable medium and symbiotic fungi for tissue culture of Paphiopedilum dianthum and to establish a rapid propagation system of Paphiopedilum,so as to promote the development of planting industry and commercial application of Paphiopedilum.[Methods]Using P.dianthum as an experimental materials,the optimal culture medium for P.dianthum was selected from DE medium,Harvais modified medium and(bark+DE)liquid medium.Then,six fungal species isolated from the original habitat soil of Paphiopedilum were co-cultured with P.dianthum on the selected optimal culture medium.By comparing the morphology,the growth amount and the physiological indexes,such as chlorophyll content,soluble sugar content and root vitality of tissue culture plantlets of P.dianthum co-cultured with the six fungal species,the suitable fungal strains for symbiosis with P.dianthum were screened out.[Results]In three kinds of culture media,P.dianthum grew normally.The growth rate was higher than that in the mediums containing DE,and there were no significant differences in the content of soluble sugar from the leaves of tissue culture plantlets,which were symbiotic with the six different fungi species.Among the six fungi species growing on the optimal medium,the growing rate of XH-A was the fastest,and that of YY001 was the slowest.The growth and physiological indexes of P.dianthum co-cultured with DY1 in DE medium were higher than those in the control group.[Conclusions]DE medium was the optimal tissue culture medium for P.dianthum,and the optimal symbiotic fungus was DY1.
