A new cyclopeptide as well as a known cyclopeptide were isolated from the whole plants of Dianthus caryophyllus. Their structures were elucidated as cyclo （-Thr-Gly-Pro-Tyr-Phe-） （1） and cyclo （-Ala-Gly-Ser-IIe-P...A new cyclopeptide as well as a known cyclopeptide were isolated from the whole plants of Dianthus caryophyllus. Their structures were elucidated as cyclo （-Thr-Gly-Pro-Tyr-Phe-） （1） and cyclo （-Ala-Gly-Ser-IIe-Phe-Phe-） （2） by means of chemical and spectroscopic methods including MS, 1D and 2D NMR techniques.展开更多
An efficient procedure was developed for in vitro callus induction, proliferation and regeneration of carnation cultivar (Dianthus caryophyllus L.) using leaf, nodal and inter-nodal explants on Murashige and Skoog’s ...An efficient procedure was developed for in vitro callus induction, proliferation and regeneration of carnation cultivar (Dianthus caryophyllus L.) using leaf, nodal and inter-nodal explants on Murashige and Skoog’s medium (MS) supplemented with exogenous plant growth regulators. For morphogenic callus induction and proliferation from various explants, MS medium supplemented with 3.0 mg/l 2,4-D was highly efficient with 100% callus induction frequency from inter-nodal explants. Leaf explants showed quicker response than nodal and inter-modal explants, for callus initiation within 6 days of inoculation. Best grown callus was obtained from leaf explant. The leaf-derived callus was maintained up to several weeks, which indicated that 8-week incubation period was the most suitable for obtaining well proliferated, morphogenic callus. Temperature variation also affected the growth of in vitro induced morphogenic callus from various explants. Results have shown that 27°C proved to be the best temperature for morphogenic callus induction and proliferation from leaf and inter-nodal explants. Among the auxin-cytokinin combination, MS medium containing 1.0 mg/l N(6)-benzylaminopurin (BAP) and 2.0 mg/l NAA showed the highest efficiency of callus initiation and proliferation from leaf, nodal and inter-nodal explants. Light conditions proved better for callogenesis and proliferation from leaf, nodal and inter-nodal explants. Regeneration response from well grown morphogenic callus was prominent on MS medium supplemented with 3.0 mg/l BAP alone and 1.0 mg/l NAA with 3.0 mg/l BAP.展开更多
This study addressed the differences in microsporogenesis between male sterile and fertile lines of Chinese pink. The microsporogenesis processes of male sterile and fertile lines were histologically examined in squas...This study addressed the differences in microsporogenesis between male sterile and fertile lines of Chinese pink. The microsporogenesis processes of male sterile and fertile lines were histologically examined in squashed pollen grains and in paraffin embedded sections. A stable male-sterile line （H-37B） was obtained following six generations of inbreeding in a self-fertile line, followed by two generations of backcrossing. In the corresponding fertile line, development of the mature pollen grains was followed through the initiation of the sporogenous cell, microsporocyte formation, and the tetrad developmental period. In the male-sterile line, abortion of the developing pollen grains was observed to take place at various stages, namely, sporogenous cell growth, mother cell meiosis, and tetrad transformation to the uninuclear state. The pollen grains of the fertile line were spheroid, turgid, and viable. By contrast, the male-sterile line produced pollen that was irregular in shape, empty, and nonviable. The abortion of the microspore in the male-sterile line appeared to relate to abnormal growth of the tapetum layer.展开更多
A cDNA clone encoding a putative EBF-like protein （DCEBF1）was obtained from total RNA isolated from senescing carnation （Dianthus caryophyllus L.） petals using reverse transcription PCR and rapid-amplification of ...A cDNA clone encoding a putative EBF-like protein （DCEBF1）was obtained from total RNA isolated from senescing carnation （Dianthus caryophyllus L.） petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of l 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBFI expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.展开更多
Phenol composition of two carnation （Dianthus caryophyllus） cvs ＂Gloriana＂ and ＂Roland＂, partially and highly resistant to F. oxysporum f. sp. dianthi, respectively, has been investigated with the aim of assessi...Phenol composition of two carnation （Dianthus caryophyllus） cvs ＂Gloriana＂ and ＂Roland＂, partially and highly resistant to F. oxysporum f. sp. dianthi, respectively, has been investigated with the aim of assessing the possible role of phenolic constituents both as biochemical markers of resistance and antifungal molecules. Analyses were performed through chromatography, NMR and MS methods. The results indicate that protocatechuic and vanillic acids are present in healthy and infected tissues of both cvs; the flavonol glycoside peltatoside. 2,6-Dimethoxybenzoic acid was detected in small amounts only in infected ＂Gloriana＂ cv while the highly resistant ＂Roland＂ cv contained the flavone datiscetin. This latter compound, due to its noticeable antifungal activity evidenced in in vitro trials, could be conceived both as a biochemical marker of resistance towards this pathogen and as a phytoanticipin.展开更多
基金supported by the Natural Science Foundation of Ynunan Province(No.2007B021M)Yurman University(No.2003Q008A).
文摘A new cyclopeptide as well as a known cyclopeptide were isolated from the whole plants of Dianthus caryophyllus. Their structures were elucidated as cyclo （-Thr-Gly-Pro-Tyr-Phe-） （1） and cyclo （-Ala-Gly-Ser-IIe-Phe-Phe-） （2） by means of chemical and spectroscopic methods including MS, 1D and 2D NMR techniques.
文摘An efficient procedure was developed for in vitro callus induction, proliferation and regeneration of carnation cultivar (Dianthus caryophyllus L.) using leaf, nodal and inter-nodal explants on Murashige and Skoog’s medium (MS) supplemented with exogenous plant growth regulators. For morphogenic callus induction and proliferation from various explants, MS medium supplemented with 3.0 mg/l 2,4-D was highly efficient with 100% callus induction frequency from inter-nodal explants. Leaf explants showed quicker response than nodal and inter-modal explants, for callus initiation within 6 days of inoculation. Best grown callus was obtained from leaf explant. The leaf-derived callus was maintained up to several weeks, which indicated that 8-week incubation period was the most suitable for obtaining well proliferated, morphogenic callus. Temperature variation also affected the growth of in vitro induced morphogenic callus from various explants. Results have shown that 27°C proved to be the best temperature for morphogenic callus induction and proliferation from leaf and inter-nodal explants. Among the auxin-cytokinin combination, MS medium containing 1.0 mg/l N(6)-benzylaminopurin (BAP) and 2.0 mg/l NAA showed the highest efficiency of callus initiation and proliferation from leaf, nodal and inter-nodal explants. Light conditions proved better for callogenesis and proliferation from leaf, nodal and inter-nodal explants. Regeneration response from well grown morphogenic callus was prominent on MS medium supplemented with 3.0 mg/l BAP alone and 1.0 mg/l NAA with 3.0 mg/l BAP.
文摘This study addressed the differences in microsporogenesis between male sterile and fertile lines of Chinese pink. The microsporogenesis processes of male sterile and fertile lines were histologically examined in squashed pollen grains and in paraffin embedded sections. A stable male-sterile line （H-37B） was obtained following six generations of inbreeding in a self-fertile line, followed by two generations of backcrossing. In the corresponding fertile line, development of the mature pollen grains was followed through the initiation of the sporogenous cell, microsporocyte formation, and the tetrad developmental period. In the male-sterile line, abortion of the developing pollen grains was observed to take place at various stages, namely, sporogenous cell growth, mother cell meiosis, and tetrad transformation to the uninuclear state. The pollen grains of the fertile line were spheroid, turgid, and viable. By contrast, the male-sterile line produced pollen that was irregular in shape, empty, and nonviable. The abortion of the microspore in the male-sterile line appeared to relate to abnormal growth of the tapetum layer.
基金supported by the National Natural Science Foundation of China (30800758 and 30972410)SRF for ROCS,SEM,Fok Ying Tung Education Foundation (104031),Chinathe Plant Biology National Experimental Teaching Demonstration Center of South China Agricultural University,China
文摘A cDNA clone encoding a putative EBF-like protein （DCEBF1）was obtained from total RNA isolated from senescing carnation （Dianthus caryophyllus L.） petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of l 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBFI expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.
文摘Phenol composition of two carnation （Dianthus caryophyllus） cvs ＂Gloriana＂ and ＂Roland＂, partially and highly resistant to F. oxysporum f. sp. dianthi, respectively, has been investigated with the aim of assessing the possible role of phenolic constituents both as biochemical markers of resistance and antifungal molecules. Analyses were performed through chromatography, NMR and MS methods. The results indicate that protocatechuic and vanillic acids are present in healthy and infected tissues of both cvs; the flavonol glycoside peltatoside. 2,6-Dimethoxybenzoic acid was detected in small amounts only in infected ＂Gloriana＂ cv while the highly resistant ＂Roland＂ cv contained the flavone datiscetin. This latter compound, due to its noticeable antifungal activity evidenced in in vitro trials, could be conceived both as a biochemical marker of resistance towards this pathogen and as a phytoanticipin.
