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Teaching Reform of Veterinary Microbiology Based on the Hybrid Teaching of Ideology and Politics
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作者 Jiedan Liao 《Journal of Contemporary Educational Research》 2024年第8期302-310,共9页
The veterinary microbiology teaching team thoroughly implements the national education policy,and iteratively promotes the implementation of the teaching reform of Sanquan education.The teaching team is focused on a“... The veterinary microbiology teaching team thoroughly implements the national education policy,and iteratively promotes the implementation of the teaching reform of Sanquan education.The teaching team is focused on a“student-centered”approach to education,emphasizing the foundation of“cultivating people and nurturing the root and soul of the nation.”By integrating“knowledge exploration,skill training,and value shaping”into the talent training plan,they are reshaping curriculum objectives and emphasizing ideological education in the syllabus.The teaching content system is being reconstructed,combining multiple interactions of blended teaching with the“six-in-one”ideological education model,to achieve the educational goals of the new agricultural“in-class-extracurricular”approach.This effort provides an empirical basis for the ideological and political teaching reform of the veterinary microbiology curriculum. 展开更多
关键词 Veterinary microbiology Ideological and political teaching Educational reform
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Hybrid Teaching Reform of Veterinary Microbiology Based on the Combination of Virtual Simulation and Problem-Based Learning
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作者 Jiedan Liao 《Journal of Contemporary Educational Research》 2024年第9期1-9,共9页
The Veterinary Microbiology course is centered around the diagnosis and testing of pathogenic microorganisms,with the core value of“moral education and character development.”It reconstructs multidimensional teachin... The Veterinary Microbiology course is centered around the diagnosis and testing of pathogenic microorganisms,with the core value of“moral education and character development.”It reconstructs multidimensional teaching resources by integrating disciplinary achievements with clinical cases and implements a hybrid teaching approach combining virtual simulation and problem-based learning(PBL)through the“three stages+four models+three reflections”framework.Dual-qualification teachers employ various teaching methods,create a“six-in-one”model for ideological and political education,and conduct formative assessments based on the principles of diversified objectives and process emphasis.The hybrid teaching reform addresses issues such as fragmented knowledge,insufficient class hours,weak animal disease diagnostic abilities among students,limited application and expansion of knowledge points,and students’lack of proactive critical thinking skills.The application of hybrid teaching has shown significant advantages and effectiveness,providing a reference for teaching reform in similar microbiology courses. 展开更多
关键词 Veterinary microbiology Hybrid teaching Virtual simulation Problem-based learning Teaching reform
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Exploration of Ideological and Political Education in Veterinary Microbiology Experimental Technology
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作者 Jiedan Liao 《Journal of Contemporary Educational Research》 2024年第9期40-46,共7页
The veterinary microbiology experimental course plays a key role in education and practice,integrating“knowledge,skills,and qualities”into the talent cultivation program.The syllabus has been revised to emphasize th... The veterinary microbiology experimental course plays a key role in education and practice,integrating“knowledge,skills,and qualities”into the talent cultivation program.The syllabus has been revised to emphasize the course’s ideological and political education objectives.The extraction of ideological and political elements focuses on developing a curriculum that includes ideological and political teaching materials and cases,ensuring coherence across classroom teaching,social practice,and online courses.By organically integrating ideological and political elements within stories,along with professional knowledge,operational skills,and social practice,the course aims to enhance the appeal and effectiveness of ideological and political education in the experimental course. 展开更多
关键词 Veterinary microbiology Experimental Technology Ideological and political education EXPLORATION
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Food Microbiology and Safety 实验课的教改探索 被引量:1
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作者 张毅 郭亚辉 谢云飞 《食品与发酵工业》 CAS CSCD 北大核心 2023年第8期351-356,共6页
该文作者所在大学与新西兰梅西大学合作开展了食品科学与工程专业国际化培养本科教育项目,全英文课程Food Microbiology and Safety是该项目的核心课程之一。该实验课以应用性为前提,以课程思政为引领,强调食品微生物学与食品安全学的融... 该文作者所在大学与新西兰梅西大学合作开展了食品科学与工程专业国际化培养本科教育项目,全英文课程Food Microbiology and Safety是该项目的核心课程之一。该实验课以应用性为前提,以课程思政为引领,强调食品微生物学与食品安全学的融合,中外师资合作开展了系列教学探索与实践。课程设计以食品产品和食品生产为情境,以微生物安全为话题,模拟真实生产场景,丰富内涵和拓展外延;自主设计实验激发主观能动性、自主规划进程锻炼统筹能力、线上资源平台支撑自主学习与评价、随机分组培养团队合作能力。实践表明,课程有效实现了食品微生物学与食品安全学的有机融合,目标驱动型实验教学过程更利于激发学生的主动性,大量综合性和设计性实验提高了课程的两性一度。与同期对照班级相比,该项目所培养学生具有更强的专业素养和实践能力,为食品专业其他实验课程的教学改革提供了参考。 展开更多
关键词 国际化 人才培养 食品微生物与安全 实验课程 教学改革
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Construction Method of Ideological and Political Theories Teaching in the Course of Environmental Microbiology for Environmental and Ecological Engineering Major 被引量:1
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作者 Weishuang TONG Yanjiao LI +4 位作者 Jiafei SU Xining GENG Guang QI Pengqiang YAO Zhilu ZHANG 《Asian Agricultural Research》 2023年第12期55-58,共4页
Environmental Microbiology is a professional basic course in the curriculum system of Environmental and Ecological Engineering major,which follows the requirements of ideological and political theories teaching in the... Environmental Microbiology is a professional basic course in the curriculum system of Environmental and Ecological Engineering major,which follows the requirements of ideological and political theories teaching in the course in higher education,and should be implemented in every course.Therefore,the construction of ideological and political theories teaching in the course should be an important task in the course construction of Environmental Microbiology.In view of the curriculum characteristics of Environmental Microbiology,such as multi-disciplinary feature,wide coverage and rapid development,it is an effective method for the construction of ideological and political theories teaching in the course of Environmental Microbiology by fully tapping the ideological and political elements of the curriculum,such as patriotism,scientific spirit and practical innovation,and integrating the ideological and political theories teaching in the course into the whole curriculum design and classroom teaching construction. 展开更多
关键词 ENVIRONMENTAL microbiology ENVIRONMENTAL and ECOLOGICAL Engineering CURRICULUM design Ideological and POLITICAL education
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Microbiological and Physico-Chemical Analysis of the Waters of the “Mamouwol” Stream in the Urban District of Mamou (Republic of Guinea)
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作者 Mamadou Lamarana Souare Mamadou Madaniou Sow +1 位作者 Saran Camara Adama Moussa Sakho 《Journal of Water Resource and Protection》 CAS 2024年第8期559-568,共10页
Water is essential to life and to the sustainable socio-economic development of a nation, It is therefore interesting to have a better knowledge of the quality of this water. The aim was to determine the degree of mic... Water is essential to life and to the sustainable socio-economic development of a nation, It is therefore interesting to have a better knowledge of the quality of this water. The aim was to determine the degree of microbiological and physico-chemical pollution of the water of the “Mamouwol” river in the town of Mamou. To do this, we chose four (4) sampling sites spread throughout the town of Mamou. During the month of March (2024), 4 water samples were analyzed to monitor the water quality of this river. The average values of the flora analyzed show that these water are heavily contaminated with bacteria indicative of fecal and metal pollution. This study showed that sites: Mam4;Mam2 and Mam1 contain the highest loads, with Total Coliform counts ranging from 1534 CFU/100 ml to 2100 CFU/100 ml, the number of faecal coliforms varies between 526 and 1240 CFU/100 ml, and that of faecal streptococci between 526 and 841 CFU/100 ml. Metal content, BOD5 and COD all comply with the laboratory’s analysis criteria, although they vary from point to point. 展开更多
关键词 Pollution Water Watercourses Waterborne Diseases microbiological Analysis
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First identification of a novel Aichivirus D in goats with diarrhea
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作者 Chen Yang Keha-mo Abi +2 位作者 Hua Yue Falong Yang Cheng Tang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第4期1442-1446,共5页
Kobuvirus comprises 6 officially recognized species,namely Aichivirus A-F,and can be further divided into 20 genotypes through VP1 gene phylogenetic analysis(https://ictv.global/report/chapter/picornaviridae/picornavi... Kobuvirus comprises 6 officially recognized species,namely Aichivirus A-F,and can be further divided into 20 genotypes through VP1 gene phylogenetic analysis(https://ictv.global/report/chapter/picornaviridae/picornaviridae/kobuvirus).Aichivirus A in human,Aichivirus B in bovine,Aichivirus C in porcine and caprine,Aichivirus D in yak have been proved to be associated with diarrhea(Chen Y S et al.2013;Yang et al.2015;Zhu et al.2016;Zhai et al.2017;Wang et al.2020;Abi et al.2022;Yan et al.2023). 展开更多
关键词 CORN diarrhea OFFICIAL
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Microbiological Quality Assessment of Mutton in the Saaba District in Ouagadougou, Burkina Faso
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作者 Dissinviel Stéphane Kpoda Assiètta Ouattara +7 位作者 Ange Irénée Toe Dinanibè Kambire Serge Touwendsida Bagre Hawa Kagambega Ibrahim Barry Iliassou Mogmenga Cheik Amadou Tidiane Ouattara Sidiki Aboubakar Ouattara 《Advances in Microbiology》 CAS 2024年第10期532-543,共12页
Introduction: This study was carried out to assess the quality of sheep meat sold to consumers in Saaba municipality. Methods: A preliminary survey consisted of assessing hygiene and sampling meat in butchers’ stores... Introduction: This study was carried out to assess the quality of sheep meat sold to consumers in Saaba municipality. Methods: A preliminary survey consisted of assessing hygiene and sampling meat in butchers’ stores. To achieve this, 100 sales outlets were surveyed for their hygiene conditions. 25 mutton meat samples were sampled in the three villages in Saaba district and analyzed using conventional microbiological techniques. The analysis consisted of determining the microbiological characteristics. Results: According to the results of this study, 100% of the butchers surveyed were men of the region. Among these men, 13% had secondary education, 46% had primary education and 41% were illiterate. Of the 100 retail outlets visited, 96% of the sellers were unaware of the hygiene rules and the dangers of microorganisms. The microbiological analysis of the samples revealed that 100% of the meat was of unsatisfactory microbiological quality. The average contamination rate by total aerobic mesophilic flora, total coliforms, Staphylococcus aureus and yeasts and molds was 8.93 × 106, 3.12 × 105, 3.69 × 106, and 6.74 × 103 CFU/g respectively. No Salmonella strain was detected in any of the samples analyzed. Conclusion: Our results pointed out the unsatisfactory safety quality of the sheep meat sold in the sheep meat and good hygiene practices. 展开更多
关键词 Sheep Meat microbiological Analyses OUAGADOUGOU Fecal Coliforms Mesophilic Flora
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The protective role of prebiotics and probiotics on diarrhea and gut damage in the rotavirus-infected piglets
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作者 Heng Yang Xiangqi Fan +4 位作者 Xiangbing Mao Bing Yu Jun He Hui Yan Jianping Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第4期1424-1448,共25页
Rotavirus is one of the pathogenic causes that induce diarrhea in young animals, especially piglets, worldwide. However, nowadays, there is no specific drug available to treat the disease, and the related vaccines hav... Rotavirus is one of the pathogenic causes that induce diarrhea in young animals, especially piglets, worldwide. However, nowadays, there is no specific drug available to treat the disease, and the related vaccines have no obvious efficiency in some countries. Via analyzing the pathogenesis of rotavirus, it inducing diarrhea is mainly due to disturb enteric nervous system, destroy gut mucosal integrity, induce intracellular electrolyte imbalance, and impair gut microbiota and immunity. Many studies have already proved that prebiotics and probiotics can mitigate the damage and diarrhea induced by rotavirus infection in hosts. Based on these, the current review summarizes and discusses the effects and mechanisms of prebiotics and probiotics on rotavirus-induced diarrhea in piglets. This information will highlight the basis for the swine production utilization of prebiotics and probiotics in the prevention or treatment of rotavirus infection in the future. 展开更多
关键词 diarrhea PIGLET PREBIOTICS PROBIOTICS ROTAVIRUS
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A CRISPR/Cas12a-based platform for rapid on-site bovine viral diarrhea virus diagnostics
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作者 Meixi Wang Jitao Chang +11 位作者 Yuxin Han Chaonan Wang Songkang Qin Jun Wang Lulu Zhang Yuanmao Zhu Fei Xue Fang Wang Hongliang Chai Yulong Wang Xinjie Wang Xin Yin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第8期2872-2876,共5页
Bovine viral diarrhea virus(BVDV)is a positive-sense single-stranded RNA virus,belonging to the genus Pestivirus in the Flaviviridae family(Riitho et al.2020).Currently,BVDV is divided into 3 main genotypes,BVDV-1,BVD... Bovine viral diarrhea virus(BVDV)is a positive-sense single-stranded RNA virus,belonging to the genus Pestivirus in the Flaviviridae family(Riitho et al.2020).Currently,BVDV is divided into 3 main genotypes,BVDV-1,BVDV-2,and BVDV-3,based on the genetic differences in the 5′untranslated region(5′UTR)(Muasya et al.2022). 展开更多
关键词 BVDV CRISPR/Cas diarrhea
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Lactose-free milk powder can effectively relieve diarrhea symptoms in weaning SD rats and children
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作者 Manman Liu Shiwen Han +5 位作者 Boya Li Cheng Chen Lu Yao Jung-il Kwon Juan Jin Huilian Che 《Food Science and Human Wellness》 SCIE CSCD 2024年第3期1494-1502,共9页
Diarrhea has become the leading cause of illness and death among infants and young children in developing countries.Clinically,patients with diarrhea showed damaged intestinal epithelial villi,usually accompanied by l... Diarrhea has become the leading cause of illness and death among infants and young children in developing countries.Clinically,patients with diarrhea showed damaged intestinal epithelial villi,usually accompanied by lactase deficiency.In this study,we evaluated the therapeutic effects of lactose-free milk powder on rats and children with diarrhea.Antibiotic-associated diarrhea(AAD)model was established by gavage with antibiotic mixture in SD rats,followed by administration of milk powder containing lactose or not.The results showed that lactose-free milk powder ameliorated A AD-related diarrhea symptoms,and accelerated the recovery from diarrhea.And 16S sequencing results indicated lactose-free milk powder contributed to increase theα-andβ-diversity of intestinal flora,and restore the intestinal microbiota disorder.In conclusion,our data demonstrate that lactose-free milk powder could alleviate diarrhea by restoring gut microbiota and intestinal barrier function. 展开更多
关键词 diarrhea Lactose-free Milk powder Intestinal barrier Gut microbiota
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Transcriptome analysis suggests broad jejunal alterations in Linghu’s obesity-diarrhea syndrome:A pilot study
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作者 Xiao-Tong Niu Xiang-Yao Wang +4 位作者 Yan Wang Ke Han Nan Ru Jing-Yuan Xiang En-Qiang Linghu 《World Journal of Gastroenterology》 SCIE CAS 2024年第21期2777-2792,共16页
BACKGROUND Obesity is associated with a significantly increased risk for chronic diarrhea,which has been proposed as Linghu’s obesity-diarrhea syndrome(ODS);however,its molecular mechanisms are largely unknown.AIM To... BACKGROUND Obesity is associated with a significantly increased risk for chronic diarrhea,which has been proposed as Linghu’s obesity-diarrhea syndrome(ODS);however,its molecular mechanisms are largely unknown.AIM To reveal the transcriptomic changes in the jejunum involved in ODS.METHODS In a cohort of 6 ODS patients(JOD group),6 obese people without diarrhea(JO group),and 6 healthy controls(JC group),high-throughput sequencing and bioinformatics analyses were performed to identify jejunal mucosal mRNA expression alterations and dysfunctional biological processes.In another cohort of 16 ODS patients(SOD group),16 obese people without diarrhea(SO group),and 16 healthy controls(SC group),serum diamine oxidase(DAO)and D-lactate(DLA)concentrations were detected to assess changes in intestinal barrier function.RESULTS The gene expression profiles of jejunal mucosa in the JO and JC groups were similar,with only 1 differentially expressed gene(DEG).The gene expression profile of the JOD group was significantly changed,with 411 DEGs compared with the JO group and 211 DEGs compared with the JC group,129 of which overlapped.The enrichment analysis of these DEGs showed that the biological processes such as digestion,absorption,and transport of nutrients(especially lipids)tended to be up-regulated in the JOD group,while the biological processes such as rRNA processing,mitochondrial translation,antimicrobial humoral response,DNA replication,and DNA repair tended to be down-regulated in the JOD group.Eight DEGs(CDT1,NHP2,EXOSC5,EPN3,NME1,REG3A,PLA2G2A,and PRSS2)may play a key regulatory role in the pathological process of ODS,and their expression levels were significantly decreased in ODS patients(P<0.001).In the second cohort,compared with healthy controls,the levels of serum intestinal barrier function markers(DAO and D-LA)were significantly increased in all obese individuals(P<0.01),but were higher in the SOD group than in the SO group(P<0.001).CONCLUSION Compared with healthy controls and obese individuals without diarrhea,patients with Linghu’s ODS had extensive transcriptomic changes in the jejunal mucosa,likely affecting intestinal barrier function and thus contributing to the obesity and chronic diarrhea phenotypes. 展开更多
关键词 OBESITY Chronic diarrhea JEJUNUM TRANSCRIPTOME Intestinal barrier
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Microbiologically influenced corrosion resistance enhancement of coppercontaining high entropy alloy Fe_(x)Cu_(1−x)CoNiCrMn against Pseudomonas aeruginosa
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作者 Yuntian Lou Weiwei Chang +4 位作者 Yu Zhang Shengyu He Xudong Chen Hongchang Qian Dawei Zhang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第11期2488-2497,共10页
To enhance the microbiologically influenced corrosion(MIC)resistance of FeCoNiCrMn high entropy alloy(HEAs),a series of Fe_(x)Cu_((1−x))CoNiCrMn(x=1,0.75,0.5,and 0.25)HEAs were prepared.Microstructural characteristics... To enhance the microbiologically influenced corrosion(MIC)resistance of FeCoNiCrMn high entropy alloy(HEAs),a series of Fe_(x)Cu_((1−x))CoNiCrMn(x=1,0.75,0.5,and 0.25)HEAs were prepared.Microstructural characteristics,corrosion behavior(morphology observation and electrochemical properties),and antimicrobial performance of Fe_(x)Cu_((1−x))CoNiCrMn HEAs were evaluated in a medium inoculated with typical corrosive microorganism Pseudomonas aeruginosa.The aim was to identify copper-containing FeCoNiCrMn HEAs that balance corrosion resistance and antimicrobial properties.Results revealed that all Fe_(x)Cu_((1−x))CoNiCrMn(x=1,0.75,0.5,and 0.25)HEAs exhibited an FCC(face centered cubic)phase,with significant grain refinement observed in Fe_(0.75)Cu_(0.25)CoNiCrMn HEA.Electrochemical tests indicated that Fe_(0.75)Cu_(0.25)CoNiCrMn HEA demonstrated lower corrosion current density(i_(corr))and pitting potential(E_(pit))compared to other Fe_(x)Cu_((1−x))CoNiCrMn HEAs in P.aeruginosa-inoculated medium,exhibiting superior resistance to MIC.Anti-microbial tests showed that after 14 d of immersion,Fe_(0.75)Cu_(0.25)CoNiCrMn achieved an antibacterial rate of 89.5%,effectively inhibiting the adhesion and biofilm formation of P.aeruginosa,thereby achieving resistance to MIC. 展开更多
关键词 microbiologically influenced corrosion Pseudomonas aeruginosa high entropy alloys antibacterial property
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Study of the Effect of Bacterial Resistance Monitoring in Clinical Microbiology Testing
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作者 Lingfeng Lu Jie Mou +1 位作者 Kun Wu Ju Luo 《Journal of Clinical and Nursing Research》 2023年第6期131-136,共6页
Objective:To observe and analyze the effect of bacterial resistance monitoring in clinical microbiology testing.Methods:600 microbial specimens collected in our hospital in the past year(April 2021 to April 2022)were ... Objective:To observe and analyze the effect of bacterial resistance monitoring in clinical microbiology testing.Methods:600 microbial specimens collected in our hospital in the past year(April 2021 to April 2022)were used as the test subjects of this study.The specimens were divided into Group A(control group)and Group B(research group),with 300 cases in each group.Group A consisted of blood culture specimens,while Group B consisted of sputum specimens.After the tests were completed,the rates of unfavorable and favorable results,bacterial species distribution,and bacterial drug resistance of the specimens in both groups were compared.Results:Among group A specimens,29 cases were positive(9.67%)and 271 cases were negative(90.33%);among group B specimens,99 cases were positive(33.00%)and 201 cases were negative(66.00%);the difference between the two groups of data was statistically significant(P<0.05).As for the distribution of the types of bacteria,there were 472 cases of Gram-negative bacteria and 128 cases of Gram-positive bacteria.Conclusion:Bacterial resistance monitoring is helpful in clinical microbiology testing.Through proper monitoring,bacterial resistance can be well understood.In this way,patients get to receive appropriate treatment measures and suitable antibacterial prescriptions,thereby improving the patient outcome. 展开更多
关键词 Bacterial resistance monitoring Clinical microbiology testing Application value
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Frequency of Rotavirus among under 5 Children Admitted to Wad Madani Pediatric Teaching Hospital with Diarrhea, Gezira State, Sudan (2021-2022)
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作者 Rehab Ibrahim Ali Babekir Adil Mergani Babiker Bakri Yosif Mohamed Nour 《Open Journal of Medical Microbiology》 2024年第3期206-214,共9页
Rotavirus is the leading cause of acute gastroenteritis and responsible for 20% of death in children under 5 years of age approximately 111 million episodes of gastroenteritis due to Rotavirus in under 5 children, whi... Rotavirus is the leading cause of acute gastroenteritis and responsible for 20% of death in children under 5 years of age approximately 111 million episodes of gastroenteritis due to Rotavirus in under 5 children, which result in 25 million visits to clinic, 2 million hospitalization and 352,000 - 592,000 deaths. Children in the poorest countries account 82% of rotaviruses death. Diarrhea disease are major cause of death and disease among children under five years, a child on average suffers 2 to 3 attacks of diarrhea every year. Stools samples were collected from 196 children suffering from diarrhea and were tested for rotaviruses by immune chromatography test antigen (ICT-Ag), ELISA and RT PCR The data were analyzed using statistical package for the Social Sciences (SPSS). The results showed that prevalence of rotavirus was 26/196 (13.3%) by ICT Ag and ELISA and confirmed by RT-PC R. 87.8% of the children were vaccinated by Rota vaccine, 70% of their mothers not using soap for cleaning the children and thought diarrhea not infectious disease and believed it was caused by teething. In conclusion there was a decrease in prevalence of diarrhea by Rotavirus due to insertion of Rota vaccine in routine vaccination, rotavirus infection mostly occurs in poor family that not able to provide safe water and due to poor sanitation and low education of mother beside that some children suffered from malnutrition, so it recommended to incorporate ELISA as a diagnostic tool in routine diagnosis of rotavirus among children suffered from gastroenteritis. 展开更多
关键词 diarrhea ROTAVIRUS Under 5 Laboratory Diagnosis SUDAN
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Calcium/calcimimetic via calcium-sensing receptor ameliorates cholera toxin-induced secretory diarrhea in mice
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作者 Lie-Qi Tang Johnathan Fraebel +4 位作者 Shi Jin Steven P Winesett Jane Harrell Wen-Han Chang Sam Xianjun Cheng 《World Journal of Gastroenterology》 SCIE CAS 2024年第3期268-279,共12页
BACKGROUND Enterotoxins produce diarrhea through direct epithelial action and indirectly by activating the enteric nervous system.Calcium-sensing receptor(CaSR)inhibits both actions.The latter has been well documented... BACKGROUND Enterotoxins produce diarrhea through direct epithelial action and indirectly by activating the enteric nervous system.Calcium-sensing receptor(CaSR)inhibits both actions.The latter has been well documented in vitro but not in vivo.The hypothesis to be tested was that activating CaSR inhibits diarrhea in vivo.AIM To determine whether CaSR agonists ameliorate secretory diarrhea evoked by cholera toxin(CTX)in mice.METHODS CTX was given orally to C57BL/6 mice to induce diarrhea.Calcium and calci-mimetic R568 were used to activate CaSR.To maximize their local intestinal actions,calcium was administered luminally via oral rehydration solution(ORS),whereas R568 was applied serosally using an intraperitoneal route.To verify that their actions resulted from the intestine,effects were also examined on Cre-lox intestine-specific CaSR knockouts.Diarrhea outcome was measured biochemically by monitoring changes in fecal Cl-or clinically by assessing stool consistency and weight loss.RESULTS CTX induced secretory diarrhea,as evidenced by increases in fecal Cl-,stool consistency,and weight loss following CTX exposure,but did not alter CaSR,neither in content nor in function.Accordingly,calcium and R568 were each able to ameliorate diarrhea when applied to diseased intestines.Intestinal CaSR involvement is suggested by gene knockout experiments where the anti-diarrheal actions of R568 were lost in intestinal epithelial CaSR knockouts(villinCre/Casrflox/flox)and neuronal CaSR knockouts(nestinCre/Casrflox/flox).CONCLUSION Treatment of acute secretory diarrheas remains a global challenge.Despite advances in diarrhea research,few have been made in the realm of diarrhea therapeutics.ORS therapy has remained the standard of care,although it does not halt the losses of intestinal fluid and ions caused by pathogens.There is no cost-effective therapeutic for diarrhea.This and other studies suggest that adding calcium to ORS or using calcimimetics to activate intestinal CaSR might represent a novel approach for treating secretory diarrheal diseases. 展开更多
关键词 CHOLERA Enteric nervous system Secretory diarrhea Oral rehydration solution Calcium-sensing receptor Gene knockout
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Relationship between dietary fiber intake and chronic diarrhea in adults
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作者 Lin Wang Yan Li +1 位作者 Yu-Jing Zhang Li-Hua Peng 《World Journal of Clinical Cases》 SCIE 2024年第19期3692-3700,共9页
BACKGROUND Dietary fiber is essential for human health and can help reduce the symptoms of constipation.However,the relationship between dietary fiber and diarrhea is,poorly understood.AIM To evaluate the relationship... BACKGROUND Dietary fiber is essential for human health and can help reduce the symptoms of constipation.However,the relationship between dietary fiber and diarrhea is,poorly understood.AIM To evaluate the relationship between dietary fiber and chronic diarrhea.METHODS This retrospective study was conducted using data from the United States National Health and Nutrition Examination Survey,conducted between 2005 and 2010.Participants over the age of 20 were included.To measure dietary fiber consumption,two 24-hour meal recall interviews were conducted.The independent relationship between the total amount of dietary fiber and chronic diarrhea was evaluated with multiple logistic regression and interaction analysis.RESULTS Data from 12829 participants were analyzed.Participants without chronic diarrhea consumed more dietary fiber than participants with chronic diarrhea(29.7 vs 28.5,P=0.004).Additionally,in participants with chronic diarrhea,a correlation between sex and dietary fiber intake was present:Women who consume more than 25 g of dietary fiber daily can reduce the occurrence of chronic diarrhea.CONCLUSION Dietary fiber can reduce the occurrence of chronic diarrhea. 展开更多
关键词 Fiber Chronic diarrhea National Health and Nutrition Examination Survey SEX PREBIOTICS
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Physico-Chemical and Microbiological Study of Fresh Pineapple(Baronne de Guinée)Grown in the Sub-prefectures of:Maferinyah and Friguiagbé(Forécariah and Kindia Prefectures),Republic of Guinea
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作者 Sâa Gérard Tolno Haicha Cissé +3 位作者 Amadou Sylla Mamadou Madaniou Sow Adama Moussa Sakho Lounceny Traore 《Journal of Environmental Science and Engineering(A)》 CAS 2024年第6期211-217,共7页
Pineapple fruit in the Republic of Guinea is currently less competitive in the West African sub-region due to the nutritional quality of the product.Thus,it is normal to review certain parameters such as:sensory,physi... Pineapple fruit in the Republic of Guinea is currently less competitive in the West African sub-region due to the nutritional quality of the product.Thus,it is normal to review certain parameters such as:sensory,physico-chemical in order to improve this classification.To do this,instead of buying samples on the market,the aim is to analyze fresh samples harvested in the production fields and send them to the laboratory for analysis of certain physico-chemical and microbiological parameters.Analytical results showed that the Brix content in fields I and II at Maferinyah(Forécariah)was higher(14.35 and 16.5)than that found in fields I and II at Friguiagbé(Kindia)(14.2 and 12.4),The pH of fruit from both localities varies between 3.48 and 3.56,with the highest acidity value found in Field I at Friguiagbé(Kindia)2.16 and the lowest value in Field II at Maferinyah(Forécariah)0.46.Moisture contents for both localities ranged from 82.56 to 89.5,with the highest value found at Field I in Maferinyah(Forécariah)89.5.Dry extract and ash in both localities would be in the range 10.54 to 17.45;0.33 to 1.43 respectively.The highest values for dry extract and ash were found in Fields I and II Friguiagbé(Kindia)17.45 and 1.43.The samples analyzed contained certain trace elements such as magnesium,sodium,iron and phosphorus.Microbiological analyses showed the presence of Escherichia coli(E.coli),yeast-molds,Staphylococcus aureus and the absence of salmonella. 展开更多
关键词 Fresh pineapple cultivated physicochemical study microbiology
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Microbiological Quality of “Rabilé”, a Yeast Used for Fermentation of Dolo, a Local Beer in Dédougou, Burkina Faso
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作者 Amana Metuor Dabiré Nicolas Ouédraogo +1 位作者 Cheick Alassane Djibila Damis Yves Patrik Bouniounou 《Open Journal of Applied Sciences》 2024年第4期849-864,共16页
Sorghum beer or dolo is part of the eating habits of part of the population of Dédougou because of its low price compared with industrial beers. Its production is an ancestral tradition that uses traditional equi... Sorghum beer or dolo is part of the eating habits of part of the population of Dédougou because of its low price compared with industrial beers. Its production is an ancestral tradition that uses traditional equipment and gives dolo organoleptic properties that are not found in industrial beers. The production process involves several stages, including fermentation, which itself comprises natural lactic fermentation followed by alcoholic fermentation using traditional yeasts, which are not controlled in any way. The general aim of this study is to assess the microbiological quality of these fermentative yeasts in the town of Dédougou, in order to contribute to the health safety of the population and the promotion of these local beers. Twenty samples of fermenting yeast were analyzed according to ISO standards, to isolate enterobacteria, total and faecal coliforms according to standard procedures for isolating these micro-organisms. The isolated strains were identified using the API20E gallery. Microbiological analyses revealed the presence of 51.17% enterobacteria, 45.38% total coliforms and 3.45% thermotolerant coliforms. We counted 40% Escherichia coli, 20% Enterobacter cloacae, 20% Klebsiella pneumoniae and 20% Klebsiella spp. All the strains detected are capable of surviving in hostile conditions and could harm the quality of the dolo, consumer health and cause real collective food poisoning in the town of Dédougou. This enabled us to assess the microbial quality of these yeasts and to propose more suitable measures for producing and preserving dolo under hygienic conditions to protect consumer health. 展开更多
关键词 Dolo YEAST Rabilé microbiological Quality BACTERIA
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Assessment of the triglyceride glucose index in adult patients with chronic diarrhea and constipation
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作者 Jing-Yi Zhu Mu-Yun Liu Chang Sun 《World Journal of Clinical Cases》 SCIE 2024年第6期1094-1103,共10页
BACKGROUND Accumulating evidence suggests that the gut microbiome is involved in the pathogenesis of insulin resistance(IR).However,the link between two of the most prevalent bowel disorders,chronic diarrhea and const... BACKGROUND Accumulating evidence suggests that the gut microbiome is involved in the pathogenesis of insulin resistance(IR).However,the link between two of the most prevalent bowel disorders,chronic diarrhea and constipation,and the triglyceride glucose(TyG)index,a marker of IR,has not yet been investigated.AIM To investigate the potential association between TyG and the incidence of chronic diarrhea and constipation.METHODS This cross-sectional study enrolled 2400 participants from the National Health and Nutrition Examination Survey database from 2009-2010.TyG was used as an exposure variable,with chronic diarrhea and constipation as determined by the Bristol Stool Form Scale used as the outcome variables.A demographic investigation based on TyG quartile subgroups was performed.The application of multivariate logistic regression models and weighted generalized additive models revealed potential correlations between TyG,chronic diarrhea,and constipation.Subgroup analyses were performed to examine the stability of any potential associations.RESULTS In the chosen sample,chronic diarrhea had a prevalence of 8.00%,while chronic constipation had a prevalence of 8.04%.In multiple logistic regression,a more prominent positive association was found between TyG and chronic diarrhea,particularly in model 1(OR=1.45;95%CI:1.17-1.79,P=0.0007)and model 2(OR=1.40;95%CI:1.12-1.76,P=0.0033).No definite association was observed between the TyG levels and chronic constipation.The weighted generalized additive model findings suggested a more substantial positive association with chronic diarrhea when TyG was less than 9.63(OR=1.89;95%CI:1.05-3.41,P=0.0344),and another positive association with chronic constipation when it was greater than 8.2(OR=1.74;95%CI:1.02-2.95,P=0.0415).The results of the subgroup analyses further strengthen the extrapolation of these results to a wide range of populations.CONCLUSION Higher TyG levels were positively associated with abnormal bowel health. 展开更多
关键词 Triglyceride glucose index National Health and Nutrition Examination Survey Chronic diarrhea Chronic constipation Cross-sectional study Bowel health
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