Hemorrhage control requires hemostatic materials that are both efective and biocompatible.Among these,diatom biosilica(DBs)could signifcantly improve hemorrhage control,but it induces hemolysis(the hemolysis rate>5...Hemorrhage control requires hemostatic materials that are both efective and biocompatible.Among these,diatom biosilica(DBs)could signifcantly improve hemorrhage control,but it induces hemolysis(the hemolysis rate>5%).Thus,the purpose of this study was to explore the infuence of Ca^(2+)biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate.Here,CaCl_(2)was added to the diatom medium under high light(cool white,fuorescent lamps,67.5µmol m^(−2) s^(−1)),producing Ca-DBs-3 with a particle size of 40-50μm and a Ca^(2+)content of Ca-DBs-3 obtained from the higher concentration CaCl_(2)group(6.7 mmol L^(−1))of 0.16%.The liquid absorption capacity of Ca-DBs-3 was 30.43±0.57 times its dry weight;the in vitro clotting time was comparable to QuikClot®zeolite;the hemostatic time and blood loss using the rat tail amputation model were 36.40±2.52 s and 0.39±0.12 g,which were 40.72%and 19.50%of QuikClot®zeolite,respectively.Ca-DBs-3 showed no apparent toxicity to L929 cells(cell viability>80%)and was non-hemolysis(the hemolysis rate<2%).This study prepared Ca-DBs-3 with a rapid hemostatic efect and good biocompatibility,providing a path to develop diatom biosilica hemostatic materials.展开更多
Diatom frustules,considered as novel bio-functional materials,display a diversity of patterns and unique micro-and nanostructures which may be useful in many areas of application.Existing devices directly use the orig...Diatom frustules,considered as novel bio-functional materials,display a diversity of patterns and unique micro-and nanostructures which may be useful in many areas of application.Existing devices directly use the original structure of the biosilica frustules,limiting their function and structural scale.Current research into the shapes,materials and structural properties of frustules are considered;a series of frustule processing methods including structure processing,material modification,bonding and assembly techniques are reviewed and discussed.The aim is to improve the function of diatom frustules allowing them to meet the design requirements of different types of micro devices.In addition,the importance of the comprehensive use of diatom processing methods in device research is discussed using biosensors and solar cells as examples,and the potential of bio-manufacturing technology based on diatom frustules is examined.展开更多
基金The work was supported by National Natural Science Foundation of China(U22A20588,82172095)Shandong Provincial Natural Science Foundation(ZR2019QD005)Qingdao Science and Technology Demonstration and Guidance Project(20-3-4-50-nsh).
文摘Hemorrhage control requires hemostatic materials that are both efective and biocompatible.Among these,diatom biosilica(DBs)could signifcantly improve hemorrhage control,but it induces hemolysis(the hemolysis rate>5%).Thus,the purpose of this study was to explore the infuence of Ca^(2+)biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate.Here,CaCl_(2)was added to the diatom medium under high light(cool white,fuorescent lamps,67.5µmol m^(−2) s^(−1)),producing Ca-DBs-3 with a particle size of 40-50μm and a Ca^(2+)content of Ca-DBs-3 obtained from the higher concentration CaCl_(2)group(6.7 mmol L^(−1))of 0.16%.The liquid absorption capacity of Ca-DBs-3 was 30.43±0.57 times its dry weight;the in vitro clotting time was comparable to QuikClot®zeolite;the hemostatic time and blood loss using the rat tail amputation model were 36.40±2.52 s and 0.39±0.12 g,which were 40.72%and 19.50%of QuikClot®zeolite,respectively.Ca-DBs-3 showed no apparent toxicity to L929 cells(cell viability>80%)and was non-hemolysis(the hemolysis rate<2%).This study prepared Ca-DBs-3 with a rapid hemostatic efect and good biocompatibility,providing a path to develop diatom biosilica hemostatic materials.
基金supported by the National Natural Science Foundation of China (50805005,51075020)the National High Technology Research and Development Program of China (2009AA043804)the Doctoral Candidate Academic Newcomer Award of the Ministry of Education of China
文摘Diatom frustules,considered as novel bio-functional materials,display a diversity of patterns and unique micro-and nanostructures which may be useful in many areas of application.Existing devices directly use the original structure of the biosilica frustules,limiting their function and structural scale.Current research into the shapes,materials and structural properties of frustules are considered;a series of frustule processing methods including structure processing,material modification,bonding and assembly techniques are reviewed and discussed.The aim is to improve the function of diatom frustules allowing them to meet the design requirements of different types of micro devices.In addition,the importance of the comprehensive use of diatom processing methods in device research is discussed using biosensors and solar cells as examples,and the potential of bio-manufacturing technology based on diatom frustules is examined.
