The degradation potential of a diesel-degrading bacteria(HDMP1) isolated from the oil-contaminated marine environment was explored systematically by analyzing the environmental conditions and synergistic action with o...The degradation potential of a diesel-degrading bacteria(HDMP1) isolated from the oil-contaminated marine environment was explored systematically by analyzing the environmental conditions and synergistic action with other dieseldegrading bacteria. The result indicated that HDMP1 was confirmed as a strain of Halomonas by the method of strain identification. And, HDMP1 showed good diesel degradation performance with a diesel degradation rate of up to 79.59%after 7 days. By analyzing the effect of environmental conditions on HDMP1, the best carbon and nitrogen sources were found to be lactose and peptone, respectively, at a pH value of 7.5 and a salinity of 4 g/(100 mL). Additionally, the synergistic effect of HDMP1 combined with other diesel-degrading bacteria was analyzed by orthogonal experimental design. The inocula of HDMP1, HDMP2, HDMP3 and HDMB3 were optimized, with the best results equating to 0.4%, 0.1%, 0.4% and0.9% in 100 mL of MSM, respectively, while the degradation rate of diesel was identified to be 73.5% within 5 days in the presence of optimum inocula.展开更多
基金financially supported by the Scientific Research Fund Project of National Natural Science Foundation of China (Grant No. 51408347)the Open Research Fund Program of Shandong Provincial Key Laboratory of Oilfield Produced Water Treatment and Environmental Pollution Control (Sinopec Petroleum Engineering Corporation) (No.201801)+2 种基金the SDUST Young Teachers Teaching Talent Training Plan (BJRC20170502)the SDUST Graduate Tutor Guidance Ability Promotion Project (KDYC17023)the Sinopec Petroleum Engineering Corporation Key Scientific and Technological Project (JP15036)
文摘The degradation potential of a diesel-degrading bacteria(HDMP1) isolated from the oil-contaminated marine environment was explored systematically by analyzing the environmental conditions and synergistic action with other dieseldegrading bacteria. The result indicated that HDMP1 was confirmed as a strain of Halomonas by the method of strain identification. And, HDMP1 showed good diesel degradation performance with a diesel degradation rate of up to 79.59%after 7 days. By analyzing the effect of environmental conditions on HDMP1, the best carbon and nitrogen sources were found to be lactose and peptone, respectively, at a pH value of 7.5 and a salinity of 4 g/(100 mL). Additionally, the synergistic effect of HDMP1 combined with other diesel-degrading bacteria was analyzed by orthogonal experimental design. The inocula of HDMP1, HDMP2, HDMP3 and HDMB3 were optimized, with the best results equating to 0.4%, 0.1%, 0.4% and0.9% in 100 mL of MSM, respectively, while the degradation rate of diesel was identified to be 73.5% within 5 days in the presence of optimum inocula.
