为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站...为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站观察BRL细胞摄取FITC-WSC-DTPA纳米荧光探针的情况。结果表明:成功合成了游离氨基含量分别为92.7%、74.3%、1.59%的WSC-DTPA聚合物;WSC、WSC纳米粒以及WSC-DTPA纳米粒(氨基含量为92.7%,浓度在6.25μg/mL以上),随着药物浓度的增加,BRL细胞存活率均显著高于单纯照射组,差别有统计学意义(p<0.05),而游离氨基含量为1.59%的WSC-DTPA纳米粒无辐射保护作用;活细胞工作站检验结果显示2 h内WSC纳米粒、WSC-DTPA纳米粒能够进入BRL细胞,而非纳米化的WSC-DTPA聚合物无法进入细胞。展开更多
AIM:To evaluate the relationship between the signal intensity of hepatobiliary phase images on gadoxetic acid-enhanced magnetic resonance imaging(MRI)and histological grade.METHODS:Fifty-nine patients with 82 hepatoce...AIM:To evaluate the relationship between the signal intensity of hepatobiliary phase images on gadoxetic acid-enhanced magnetic resonance imaging(MRI)and histological grade.METHODS:Fifty-nine patients with 82 hepatocellular lesions were evaluated retrospectively.Hepatobiliary phase images on gadoxetic acid-enhanced MRI were classified into 3 groups:low,iso or high.Angiographyassisted computed tomography(CT)findings were also classified into 3 groups:CT during arterial portography,and CT hepatic arteriography:A:iso,iso or low;B:slightly low,iso or low;;and C:low,high.We correlated angiography-assisted CT,hepatobiliary phase findings during gadoxetic acid-enhanced MRI and histological grades.Furthermore,correlations between MRI findings and histological grade for each hemodynamic pattern were performed.Correlations among radiologicaland pathological findings were statistically evaluated using the chi-square test and Fisher's exact test.RESULTS:There was a significant correlation between histological grade and hemodynamic pattern(P<0.05).There was a significant correlation between histological grade and signal intensity in the hepatobiliary phase(P<0.05)in group A lesions.There was no significant correlation between histological grade and signal intensity in the hepatobiliary phase in group B or C lesions(P>0.05).CONCLUSION:Signal intensity in the hepatobiliary phase correlated with histological grade in the lesions that maintained portal blood flow,but did not correlate in lesions that showed decreased or defective portal blood flow.展开更多
The crystal and molecular structures of the Na[Gd Ⅲ(edta)(H_2O)_3]·5H_2O (edta=ethylenediaminetetraacetic acid) and Na_4[Gd Ⅲ(dtpa)(H_2O)]_2·14H_2O (dtpa=diethylenetriaminepentaacetic acid) complexes were ...The crystal and molecular structures of the Na[Gd Ⅲ(edta)(H_2O)_3]·5H_2O (edta=ethylenediaminetetraacetic acid) and Na_4[Gd Ⅲ(dtpa)(H_2O)]_2·14H_2O (dtpa=diethylenetriaminepentaacetic acid) complexes were determined by single-crystal X-ray structure analyses. The crystal of the Na[Gd Ⅲ(edta)(H_2O)_3]·5H_2O belongs to orthorhombic crystal system and Fdd2 space group. The crystal data are as follows: a =1.9424(7)nm,b =3.5496(12)nm,c =1.2125(4)nm,V =8.360(5)nm 3,Z =16,M =612.63,D _c=1.947 g·cm (-3),μ =3.274 mm (-1) and F (000)=4880. The final R and wR values are 0.0295 and 0.0677 for 3102 [ I>2.0σ(I) ] unique reflections,and 0.0328 and 0.0695 for all 8269 reflections,respectively. The [Gd Ⅲ(edta)(H_2O)_3]-complex anion has a nine-coordinate pseudo-monocapped square antiprismatic structure in which the nine coordinate atoms,two N and seven O are from one edta ligand and three coordinate water molecules. The crystal of the Na_4[Gd Ⅲ(dtpa)(H_2O)]_2·14H_2O belongs to monoclinic crystal system and P2(1)/n space group. The crystal data are as follows: a =1.4894(6)nm,b =1.7987(7)nm, c =1.9600(7)nm,β =99.496(7),V =5.179(3)nm3,Z=4,M =1471.44,D _c=1.841 g·cm (-3),μ =2.676 mm (-1) and F (000)=2872. The final R and wR are 0.0435 and 0.0979 for 8929 [ I>2.0σ(I) ] unique reflections,and 0.0820 and 0.1167 for all 20717 reflections,respectively. The total complex moleculue is composed of two close parts in structure. The [Gd Ⅲ(dtpa)(H_2O)] (2-) complex anion in the every part has a nine-coordinate structure with distorted monocapped square antiprismatic prism,in which the dtpa coordinates with central Gd Ⅲ ion acting as a octadentate ligand with three N atoms and five O atoms,and one water as the ninth ligand directly coordinates with central Gd Ⅲ ion.展开更多
文摘为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站观察BRL细胞摄取FITC-WSC-DTPA纳米荧光探针的情况。结果表明:成功合成了游离氨基含量分别为92.7%、74.3%、1.59%的WSC-DTPA聚合物;WSC、WSC纳米粒以及WSC-DTPA纳米粒(氨基含量为92.7%,浓度在6.25μg/mL以上),随着药物浓度的增加,BRL细胞存活率均显著高于单纯照射组,差别有统计学意义(p<0.05),而游离氨基含量为1.59%的WSC-DTPA纳米粒无辐射保护作用;活细胞工作站检验结果显示2 h内WSC纳米粒、WSC-DTPA纳米粒能够进入BRL细胞,而非纳米化的WSC-DTPA聚合物无法进入细胞。
文摘AIM:To evaluate the relationship between the signal intensity of hepatobiliary phase images on gadoxetic acid-enhanced magnetic resonance imaging(MRI)and histological grade.METHODS:Fifty-nine patients with 82 hepatocellular lesions were evaluated retrospectively.Hepatobiliary phase images on gadoxetic acid-enhanced MRI were classified into 3 groups:low,iso or high.Angiographyassisted computed tomography(CT)findings were also classified into 3 groups:CT during arterial portography,and CT hepatic arteriography:A:iso,iso or low;B:slightly low,iso or low;;and C:low,high.We correlated angiography-assisted CT,hepatobiliary phase findings during gadoxetic acid-enhanced MRI and histological grades.Furthermore,correlations between MRI findings and histological grade for each hemodynamic pattern were performed.Correlations among radiologicaland pathological findings were statistically evaluated using the chi-square test and Fisher's exact test.RESULTS:There was a significant correlation between histological grade and hemodynamic pattern(P<0.05).There was a significant correlation between histological grade and signal intensity in the hepatobiliary phase(P<0.05)in group A lesions.There was no significant correlation between histological grade and signal intensity in the hepatobiliary phase in group B or C lesions(P>0.05).CONCLUSION:Signal intensity in the hepatobiliary phase correlated with histological grade in the lesions that maintained portal blood flow,but did not correlate in lesions that showed decreased or defective portal blood flow.
文摘The crystal and molecular structures of the Na[Gd Ⅲ(edta)(H_2O)_3]·5H_2O (edta=ethylenediaminetetraacetic acid) and Na_4[Gd Ⅲ(dtpa)(H_2O)]_2·14H_2O (dtpa=diethylenetriaminepentaacetic acid) complexes were determined by single-crystal X-ray structure analyses. The crystal of the Na[Gd Ⅲ(edta)(H_2O)_3]·5H_2O belongs to orthorhombic crystal system and Fdd2 space group. The crystal data are as follows: a =1.9424(7)nm,b =3.5496(12)nm,c =1.2125(4)nm,V =8.360(5)nm 3,Z =16,M =612.63,D _c=1.947 g·cm (-3),μ =3.274 mm (-1) and F (000)=4880. The final R and wR values are 0.0295 and 0.0677 for 3102 [ I>2.0σ(I) ] unique reflections,and 0.0328 and 0.0695 for all 8269 reflections,respectively. The [Gd Ⅲ(edta)(H_2O)_3]-complex anion has a nine-coordinate pseudo-monocapped square antiprismatic structure in which the nine coordinate atoms,two N and seven O are from one edta ligand and three coordinate water molecules. The crystal of the Na_4[Gd Ⅲ(dtpa)(H_2O)]_2·14H_2O belongs to monoclinic crystal system and P2(1)/n space group. The crystal data are as follows: a =1.4894(6)nm,b =1.7987(7)nm, c =1.9600(7)nm,β =99.496(7),V =5.179(3)nm3,Z=4,M =1471.44,D _c=1.841 g·cm (-3),μ =2.676 mm (-1) and F (000)=2872. The final R and wR are 0.0435 and 0.0979 for 8929 [ I>2.0σ(I) ] unique reflections,and 0.0820 and 0.1167 for all 20717 reflections,respectively. The total complex moleculue is composed of two close parts in structure. The [Gd Ⅲ(dtpa)(H_2O)] (2-) complex anion in the every part has a nine-coordinate structure with distorted monocapped square antiprismatic prism,in which the dtpa coordinates with central Gd Ⅲ ion acting as a octadentate ligand with three N atoms and five O atoms,and one water as the ninth ligand directly coordinates with central Gd Ⅲ ion.
