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Compositional and Structural Difference of Fulvic Acid from Black Soil Applied with Different Organic Materials: Assessment After Three Years 被引量:3
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作者 LI Jian-ming WU Jing-gui 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第10期1865-1871,共7页
Knowledge of different effects of various organic materials on soil humic substance is important for both environmental safety and sustainable agriculture.A pot experiment was conducted at Jilin Agricultural Universit... Knowledge of different effects of various organic materials on soil humic substance is important for both environmental safety and sustainable agriculture.A pot experiment was conducted at Jilin Agricultural University,Jilin Province in northeast China to discover the influence of herb residue,animal excrement,woody residue,animal remnant on fulvic acid(FA) composition and structure using differential thermal analysis-thermogravimetric(DTA-TG),fourier transform infrared spectroscopy(FTIR) and elemental analysis.DTA-TG showed the range of peak temperature in the first exothermic reaction increased following the trend: CK〉herb residue〉animal excrement〉woody residue=animal remnant,and the most weight loss was observed in animal excrement.Moreover,the second exothermic reaction of CK-and animal excrement-FA was presented as double peaks,the order of weight loss in that area was animal remnant〉CK〉woody residue〉animal excrement〉herb residue.According to FTIR,herb residue displayed higher adsorption intensity at 2 950,1 420,1 240 and 1 030 cm-1,animal excrement was in reverse.At the same time,herb residue-and animal excrement-FA had an absorption peak at 1 720 cm-1,while other organic materials didn't have this peak.As elemental analysis showed,FA isolated from various treatments was significantly distinct.It was clearly shown from our results that FA composition and structure in amended soils may be affected in different ways and at various extents on dependence of the nature and origin of amendment. 展开更多
关键词 different organic materials fulvic acid DTA-TG FTIR elemental analysis
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Valproic acid alters differential protein expression in SH-SY5Y cells
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作者 Zhong Dong Ying Wang Ming Chang Guoyi Li Linsen Hu 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第27期2134-2139,共6页
This study sought to identify differentially expressed proteins in SH-SY5Y cells treated with valproic acid, using two-dimensional difference gel electrophoresis analysis. Three proteins were unambi-guously identified... This study sought to identify differentially expressed proteins in SH-SY5Y cells treated with valproic acid, using two-dimensional difference gel electrophoresis analysis. Three proteins were unambi-guously identified: the eukaryotic translation initiation factor 4A isoform 1 and ATP6V1B2 protein were downregulated, while the heterogeneous nuclear ribonucleoprotein K was upregulated. Moreover, all three proteins are associated with altered expression due to oxidative stress. Ma-trix-assisted laser desorption/ionization-time of flight mass spectrometry and protein immunoblotting assay confirmed the differential expression of eukaryotic translation initiation factor 4A isoform 1. The results indicate that valproic acid exerts an antioxidation effect by regulating the expression of eukaryotic translation initiation factor 4A isoform 1. 展开更多
关键词 valproic acid two-dimensional difference gel electrophoresis matrix-assisted laser desorption-ionization SH-SY5Y cells proteomics neural regeneration
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