Changes of evoked potential in different hippocampal regions induced by electrostimulation at medial mamillary nucleus of rats

BACKGROUND： Morphological data have shown that the most important afferent fibers of papillary body come from hippocampal structure.OBJECTIVE： To observe the changes of evoked potential in hippocampus and the significance after electrostimulation at medial mamillary nucleus. DESIGN： An observational control experiment.SETTING： Department of Physiology, Shenyang Medical College.MATERIALS ： Twenty-three male or female Wistar rats, 3-4 months old, weighing 270-350 g, were provided by bhe animal room of Shenyang Medical College [the license number was scxk（Liao）2003-0016]. METHODS： The Wistar rats were anaesthetized by intraperintoneal injection of 20% urethane （1 g/kg）, tracheal intubation was also given. The self-made double-pole metal stimulating electrode with the point diameter of 1 mm was inserted into medial mamillary nucleus, the wanted hippocampal guidance spot was found within the rang of the hippocampal region at the same side of tee mamillary body range （CA1-CA4）, inserted with same-core guidance electrode, a sole square-wave stimulation of wave wide 0.2 ms stimulated with electrodes at the applied intensity of 7-9 V, the evoked potential was induced through guidance electrodes, and then input to the ATAC-350 data-processing machine for memory showing wave processing, the memory recorded wave recording graph was separately drawn up by the X-Y recording instrument to observe the latency, time procedure and amplitude of the evoked potential in each hippocampal region of the rats and calculate the percentage of the evoked potential in each hippocampal region. Totally 78 guidance spots in hippocampus were recorded, including 30 positive reaction spots and 48 negative ones. MAIN OUTCOME MEASURES： ① Latency, time procedure and amplitude of the evoked potentials in each hippocampal region of rats; ②percentage of the evoked potentials in each hippocampal region; ③ the wave shapes of the evoked potentials in each hippocampal region from different arrangement in the same positive reaction spot. RESULTS ：① Of the 30 recorded positive reactions, 9 positive spots fused into the stimulated false marks because of the short latency. The analysis of variance showed that the latency had significant difference （P 〈 0.05）, time procedure had highly significant difference （P 〈 0.01 ）, but there was no significant difference in the amplitude （P 〉 0.05） among the hippampal regions.② Among the 30 positive spots, the percentage of evoked potential in the hippocampal regions were 34.5% for CA1, 2.0% for CA2, 24.1% for CA3 and 22.4% for CA4. ③ In different levels of the same positive spot, different changes of the evoked potential wave shapes could be observed, and the most obvious change was that of positive wave amplitude. At different positive spots, evoked potentials of positive phase, negative and the double-phase could be observed. CONCLUSION： There are nerve associations between mamillary body and hippocampus, afferent fibers of mamillary body come from hippocampal CA1 region are a little more.

Spatial-temporal Changes of Vegetation Cover in Guizhou Province, Southern China

Guizhou Province is an important karst area in the world and a fragile ecological area in China. Ecological risk assessment is very necessary to be conducted in this region. This study investigates different characteristics of the spatial-temporal changes of vegetation cover in Guizhou Province of Southern China using the data set of SPOT VEGETATION(1999–2015) at spatial resolution of 1-km and temporal resolution of 10-day. The coefficient of variation, the Theil-Sen median trend analysis, and the Mann-Kendall test are used to investigate the spatial-temporal change of vegetation cover and its future trend. Results show that: 1) the spatial distribution pattern of vegetation cover in Guizhou Plateau is high in the east whereas low in the west. The average annual normalized difference vegetation index(NDVI) from west to east is higher than that from south to north. 2) Average annual NDVI improved obviously in the past 17 years. The growth rate of average annual NDVI is 0.028/10 yr, which is slower than that of vegetation in the country(0.048/10 yr) from 1998 to 2007. Average annual NDVI in karst area is lower than that in non-karst area. However, the growing rate of average annual NDVI in karst area(0.030/10 yr) is faster than that in non-karst area(0.023/10 yr), indicating that vegetation coverage increases more rapidly in karst area. 3) Vegetation coverage in the study area is stable overall, but fluctuates in the local scales. 4) Vegetation coverage presents a continuous increasing trend. The Hurst exponent of NDVI in different vegetation types has an obvious threshold in various elevations. 5) The proportion of vegetation cover with sustainable increase is higher than that of vegetation cover with sustainable decrease. The improvement in vegetation cover may expand to most parts of the study area.

Land cover change in different altitudes of Guizhou-Guangxi karst mountain area, China： patterns and drivers

Topography, especially altitude, will influence the way, process and characteristics of land cover changes in mountainous area, simultaneously, the vertical difference of land cover changes will affect soil quality and regional ecological environment. Therefore, the gradient relationship analysis between land cover changes and altitude is very important for regional sustainability. This study investigated land cover dynamics based on land cover data from a typical mountainous area in the Guizhou-Guangxi karst mountain area, China, in 2000 and 2010, then explored the relationship between altitude and land cover change and analyzed different drivers of land cover change at different altitudes. Our findings are as follows. 1) From 2000 to 2010, the total area of land cover transition was 7167.04 km^2 or 2.8% of the region. The increasing area of build-up land(926.23 km^2) was larger than that of forest(859.38 km^2), suggesting that the urban construction speed was higher than that of reforestation. 2) Intensity of land cover transition in northwestern Guizhou-Guangxi karst mountain area was much larger than that of southeast part and their transition trend was also significantly different, which was consistent with regional population and economy. 3) Human activity was the most dramatic at altitudes between 0–500 m. For 500–1000 m, grassland mainly converted to forest and build-up land. Area of land cover transition was the greatest between 1000–1500 m, while above 1500 m, the transition of grassland was the most obvious. 4) The drivers of land cover change varied. Land cover change was positively correlated with gross domestic product and population density but was inversely related to relief amplitude. There were correlations between land cover change and distance to roads and rivers, and their correlations varied with altitude. By revealing patterns and causes of land cover changes in different altitudes, we hope to understand the vertical dependence of land cover changes, so as to improve land productivity and protect land ecological environment scientifically.

Spatial variability of glacial changes and their effects on water resources in the Chinese Tianshan Mountains during the last five decades

Changes in glaciers in the Chinese Tianshan Mountains have been analyzed previously. However, most previous studies focused on individual glaciers and/or decentralized glacial basins. Moreover, a majority of these studies were published only in Chinese, which limited their usefulness at the international level. With this in mind, the authors reviewed the previous studies to create an overview of glacial changes in the Chinese Tianshan Mountains over the last five decades and discussed the effects of glacial changes on water resources. In response to climate change, glaciers in the Tianshan Mountains are shrinking rapidly and are ca. 20% smaller on average in the past five decades. Overall, the area reduction of glacial basins in the central part of the Chinese Tianshan Mountains is larger than that in the eastern and western parts. The spatial differentiation in glacial changes are caused by both differences in regional climate and in glacial factors. The effects of glacial changes on water resources vary in different river basins due to the differences in glacier distribution, characteristics of glacial change and proportion of the glacier meltwater in river runoff.

The digital anatomic study of the changing regularity of thoracic pedicle screw channels in different transverse screw angle

Objective To investigate the changing regularity of thoracic pedicle screw channel (PSC) in different transverse screw angle(TSA).Methods CT scan images of thoracic vertebrae of a healthy adult ware digitally analyzed by Mimics 10.01

Gravity anomaly before the Leshan M5.0 earthquake?

The North-South Seismic Belt was analyzed using gravity observation data from 2011 to 2015, and the nontidal analysis results show that there was a nonlinear gravity change at both the Chengdu and Guza seismostations one month before the Leshan M5.0 earthquake.

Shape Similarity Measures of Linear Entities

The essential of feature matching technology lies in how to measure the similarity of spatial entities.Among all the possible similarity measures,the shape similarity measure is one of the most important measures because it is easy to collect the necessary parameters and it is also well matched with the human intuition.In this paper a new shape similarity measure of linear entities based on the differences of direction change along each line is presented and its effectiveness is illustrated.

A BLOCK-CENTERED UPWIND APPROXIMATION OF THE SEMICONDUCTOR DEVICE PROBLEM ON A DYNAMICALLY CHANGING MESH

The numerical simulation of a three-dimensional semiconductor device is a fundamental problem in information science. The mathematical model is defined by an initialboundary nonlinear system of four partial differential equations: an elliptic equation for electric potential, two convection-diffusion equations for electron concentration and hole concentration, and a heat conduction equation for temperature. The first equation is solved by the conservative block-centered method. The concentrations and temperature are computed by the block-centered upwind difference method on a changing mesh, where the block-centered method and upwind approximation are used to discretize the diffusion and convection, respectively. The computations on a changing mesh show very well the local special properties nearby the P-N junction. The upwind scheme is applied to approximate the convection, and numerical dispersion and nonphysical oscillation are avoided. The block-centered difference computes concentrations, temperature, and their adjoint vector functions simultaneously.The local conservation of mass, an important rule in the numerical simulation of a semiconductor device, is preserved during the computations. An optimal order convergence is obtained. Numerical examples are provided to show efficiency and application.

Using Isotope Technology for Surface Water Environment Research in Southern Hanoi

The surface water in the southern of Hanoi capital is researched by identifying δ2H and δ18O stable isotopes together with EC, DO, BOD5, COD, TSS. Surface water samples for studying include the Red river, Nhue river and Kim Nguu river, Thanh Nhan lake, Yen So lake and Van Quan lake were collected in the dry season (April) and the rainy season (August) in 2015. The stable isotope analysis results showed that the δ18O values is a range from -42.53‰ to -64.05‰ and, the δ2H values is the range from -5.09‰ to -8.79‰ under global meteoric waterline (in the water vapor region). The δ2H results of the rivers and lakes in the dry season are more negative than the rainy season with a small difference. The δ18O results of lakes in the dry season are more negative than the rainy season, but the δ18O results of river in the dry season are more positive than the rainy season. The results of the EC, BOD, COD, DO, TSS analysis showed that surface water environment has changed clearly in the two seasons and the contamination level in the dry season is usually higher than the rainy season. The lakes and rivers strongly influenced by human activities led to seriously pollution are Van Quan lake and Yen So lake, Nhue river and Kim Nguu river.

CHANGES OF CELLULAR MORPHOLOGY AND NUCLEAR DNA CONTENT IN DIFFERENT NASOPHARYNGEAL EPITHELIUM FROM DIFFERENT PATIENTS

Cellular morphology and nuclear DNA content were measured with point-counting technique and flow cytometry (FCM) respectively in normal nasopharyngeal epithelium (NE), tissue adjacent to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). The results showed that all 20 patients with NE show ed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 patients with ATC and 2 (33%) of 6 patients with NE positive to EBVCA-IgA test showed nondiploid. A total of 19 morphometric parameters of the cell were measured and calculated. The results showed that with malignant transformation of NE, most of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal NE and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasia changed significantly as compared with those normal NE, simple hyperplasia or metaplasia and NPC groups. Cytomorphome

Rate of Phase Difference Change Estimation in Single Airborne Passive Locating System

As an important parameter in the single airborne passive locating system, the rate of phase difference change contains range information of the radio emitter. Taking single carrier sine pulse signals as an example, this article illustrates the principle of passive location through measurement of rates of phase difference change and analyzes the structure of measurement errors. On the basis of the Cramér-Rao lower bound (CRLB), an algorithm associated with time-chips is proposed to determine the rates of pha...

Detecting the storage and change on topsoil organic carbon in grasslands of Inner Mongolia from 1980s to 2010s

Soil carbon sequestration and potential has been a focal issue in global carbon research. Under the background of global change, the estimation of the size as well as its change of soil organic carbon（SOC） storage is of great importance. Based on soil data from the second national soil survey and field survey during 2011–2012, by using the regression method between sampling soil data and remote sensing data, this paper aimed to investigate spatial distribution and changes of topsoil（0–20 cm） organic carbon storage in grasslands of Inner Mongolia between the 1980 s and 2010 s. The results showed that：（1） the SOC storage in grasslands of Inner Mongolia between the 1980 s and 2010 s was estimated to be 2.05 and 2.17 Pg C, with an average density of 3.48 and 3.69 kg C·m–2, respectively. The SOC storage was mainly distributed in the typical steppe and meadow steppe, which accounted for over 98% of the total SOC storage. The spatial distribution showed a decreased trend from the meadow steppe, typical steppe to the desert steppe, corresponding to the temperature and precipitation gradient.（2） SOC changes during 1982–2012 were estimated to be 0.12 Pg C, at 7.00 g C·m–2·yr–1, which didn＇t show a significant change, indicating that SOC storage in grasslands of Inner Mongolia remained relatively stable over this period. However, topsoil organic carbon showed different trends of carbon source/sink during the past three decades. Meadow steppe and typical steppe had sequestered 0.15 and 0.03 Pg C, respectively, served as a carbon sink; while desert steppe lost 0.06 Pg C, served as a carbon source. It appears that SOC storage in grassland ecosystem may respond differently to climate change, related to vegetation type, regional climate type and grazing intensity. These results might give advice to decision makers on adopting suitable countermeasures for sustainable grassland utilization and protection.