Peripheral blood monocytes seem to be of importance for the initiation and maintenance of the psoriatic tissue reaction. Hyperproliferation of monocytopoiesis as well as functional abnormalities of monocytes in psotia...Peripheral blood monocytes seem to be of importance for the initiation and maintenance of the psoriatic tissue reaction. Hyperproliferation of monocytopoiesis as well as functional abnormalities of monocytes in psotiasis have previously been described. We sought to determine whether peripheral blood monocyte subpopulations from patients with psoriasis show altered phentypes. and how the altered展开更多
This study examined the neuroprotective effect of cluster of differentiation molecule 200 （CD200） against methamphetamine （METH）-induced neurotoxicity. In the in vitro experiment, neuron-microglia cultures were tr...This study examined the neuroprotective effect of cluster of differentiation molecule 200 （CD200） against methamphetamine （METH）-induced neurotoxicity. In the in vitro experiment, neuron-microglia cultures were treated with METH （20 μmol/L）, METH （20 μmol/L）＋CD200-Fc （10 μg/mL） or CD200-Fc （10 μg/mL）. Those untreated served as control. Microglia activation expressed as the ratio of MHC-Ⅱ/CD11b was assessed by flow cytometry. The cytokines （IL-1β, TNF-α） secreted by activated microglia were detected by enzyme-linked immunosorbent assay （ELISA）. In the in vivo experiment, 40 SD rats were divided into control, METH, METH＋CD200-Fc and CD200-Fc groups at random. Rats were intraperitoneally injected with METH （15 mg/kg 8 times at 12 h interval） in METH group, with METH （administered as the same dose and time as the METH group） and CD200-Fc （1 mg/kg at day 0, 2, 4 after METH injection） in METH＋CD200-Fc group, with CD200-Fc （1 mg/kg injected as the same time as the METH＋CD200-Fc group） or with physiological saline solution in the control group. The level of striatal dopamine （DA） in rats was measured by high-performance liquid chromatography （HPLC）. The microglial cells were immunohistochemically detected for the expression of Iba-1, a marker for microglial activation. The results showed that METH could increase the microglia activation in the neuron-microglia cultures and elevate the secretion of IL-1β and TNF-α, which could be attenuated by CD200-Fc. Moreover, CD200-Fc could partially reverse the striatal DA depletion induced by METH and reduce the number of activated microglia, i.e. Iba-1-positive cells. It was concluded that CD200 may have neuroprotective effects against METH-induced neurotoxicity by inhibiting microglial activation and reversing DA depletion in striatum.展开更多
文摘Peripheral blood monocytes seem to be of importance for the initiation and maintenance of the psoriatic tissue reaction. Hyperproliferation of monocytopoiesis as well as functional abnormalities of monocytes in psotiasis have previously been described. We sought to determine whether peripheral blood monocyte subpopulations from patients with psoriasis show altered phentypes. and how the altered
基金supported by grants from the National Natural Science Foundation of China(No.30572090 and No.30872918)
文摘This study examined the neuroprotective effect of cluster of differentiation molecule 200 （CD200） against methamphetamine （METH）-induced neurotoxicity. In the in vitro experiment, neuron-microglia cultures were treated with METH （20 μmol/L）, METH （20 μmol/L）＋CD200-Fc （10 μg/mL） or CD200-Fc （10 μg/mL）. Those untreated served as control. Microglia activation expressed as the ratio of MHC-Ⅱ/CD11b was assessed by flow cytometry. The cytokines （IL-1β, TNF-α） secreted by activated microglia were detected by enzyme-linked immunosorbent assay （ELISA）. In the in vivo experiment, 40 SD rats were divided into control, METH, METH＋CD200-Fc and CD200-Fc groups at random. Rats were intraperitoneally injected with METH （15 mg/kg 8 times at 12 h interval） in METH group, with METH （administered as the same dose and time as the METH group） and CD200-Fc （1 mg/kg at day 0, 2, 4 after METH injection） in METH＋CD200-Fc group, with CD200-Fc （1 mg/kg injected as the same time as the METH＋CD200-Fc group） or with physiological saline solution in the control group. The level of striatal dopamine （DA） in rats was measured by high-performance liquid chromatography （HPLC）. The microglial cells were immunohistochemically detected for the expression of Iba-1, a marker for microglial activation. The results showed that METH could increase the microglia activation in the neuron-microglia cultures and elevate the secretion of IL-1β and TNF-α, which could be attenuated by CD200-Fc. Moreover, CD200-Fc could partially reverse the striatal DA depletion induced by METH and reduce the number of activated microglia, i.e. Iba-1-positive cells. It was concluded that CD200 may have neuroprotective effects against METH-induced neurotoxicity by inhibiting microglial activation and reversing DA depletion in striatum.
