Electroacupuncture pretreatment exhibits antidepressive effects by regulating hippocampal proteomics in rats with chronic restraint stress

The clinical effect of electroacupuncture on depression is widely recognized. However, the signal transduction pathways and target proteins involved remain unclear. In the present study, rat models of chronic restraint stress were used to explore the mechanism by which electroacupuncture alleviates depression. Rats were randomly divided into control, model, and electroacupuncture groups. Chronic restraint stress was induced in the model and electroacupuncture groups by restraining rats for 28 days. In the electroacupuncture group, electroacupuncture pretreatment at Baihui（GV20） and Yintang（GV29） acupoints was performed daily（1 m A, 2 Hz, discontinuous wave, 20 minutes） prior to restraint for 28 days. Open field tests and body weight measurements were carried out to evaluate the depressive symptoms at specific time points. On day 28, the crossing number, rearing number, and body weights of the model group were significantly lower than those in the control group. Behavior test results indicated that rat models of depressive-like symptoms were successfully established by chronic restraint stress combined with solitary raising. On day 28, an isobaric tag for a relative and absolute quantitation-based quantitative proteomic approach was performed to identify differentially expressed proteins in hippocampal samples obtained from the model and electroacupuncture groups. The potential function of these differential proteins was predicted through the use of the Cluster of Orthologous Groups of proteins（COG） database. Twenty-seven differential proteins（uncharacteristic proteins expected） were selected from the model and electroacupuncture groups. In addition to unknown protein functions, COG are mainly concentrated in general prediction function, mechanism of signal transduction, amino acid transport and metabolism groups. This suggests that electroacupuncture improved depressive-like symptoms by regulating differential proteins, and most of these related proteins exist in nerve cells.

Spatio-temporal characteristics of acoustic emission during the deformation of rock samples with compressional and extensional en-echelon faults

The spatio-temporal characteristics of acoustic emission (AE) during the deformation of rock samples with compressional and extensional en-echelon faults have been studied. The results show that the pre-existing structure can significantly influence the patterns of AE spatial distribution. With increasing of differential stress, AE events firstly cluster around the two ends of pre-existing faults inside the jog and then along the line joining the two ends. The biggish AE events often occur around one end repeatedly. The image of AE clusters indicates the direction and the area of the fracture propagation. The direction of the macroscopic fracture in extensional and compressional jogs is perpendicular and parallel to the direction of axial stress, respectively. The weakening process before the fracturing of jog area is remarkable, and one of the typical precursors for the instability is that the cumulative frequency of AE events increases exponentially. After the fracturing of the jog the frequency and releasing strain energy of AE events decrease gradually. During the friction period, there is no precursory increasing of AE activity before the big stick-slip events. The change of b value in jog shows a typical change of decreasing tendentiously returning quickly before the instability. The decrease of b value occurs in the process of stress increasing and sometime goes down to the weakening stage, and the quick increase b values appears in a short time just before the instability. The comparative analysis shows that the difference in b value due to the different structures is larger than b value variation caused by increase of the differential stress. For the same sample, the temporal sequence of AE is strongly affected by the mechanical state, and the high loading velocity corresponds to the high release rate of strain energy and low b value. Due to its lower failure strength, the broken area is sensitive to small changes in differential stress. Therefore, it offers a potential explanation for the phenomena of precursory window or sensitive point and separation of seismic source and precursors.

Upper Limit for Rheological Strength of Crust in Continental Subduction Zone:Constraints Imposed by Laboratory Experiments

The transitional pressure of quartz coesite under the differential stress and highly strained conditions is far from the pressure of the stable field under the static pressure. Therefore, the effect of the differential stress should be considered when the depth of petrogenesis is estimated about ultrahigh pressure metamorphic (UHPM) rocks. The rheological strength of typical ultrahigh pressure rocks in continental subduction zone was derived from the results of the laboratory experiments. The results indicate the following three points. (1) The rheological strength of gabbro, similar to that of eclogite, is smaller than that of clinopyroxenite on the same condition. (2) The calculated strength of rocks (gabbro, eclogite and clinopyroxenite) related to UHPM decreases by nearly one order of magnitude with the temperature rising by 100 ℃ in the range between 600 and 900 ℃. The calculated strength is far greater than the faulting strength of rocks at 600 ℃, and is in several hundred to more than one thousand mega pascals at 700-800 ℃, which suggests that those rocks are located in the brittle deformation region at 600 ℃, but are in the semi brittle to plastic deformation region at 700-800 ℃. Obviously, the 700 ℃ is a brittle plastic transition boundary. (3) The calculated rheological strength in the localized deformation zone on a higher strain rate condition (1.6×10 -12 s -l ) is 2-5 times more than that in the distributed deformation zone on a lower strain rate condition (1.6×10 -14 s -1 ). The average rheological stress (1 600 MPa) at the strain rate of 10 -12 s -1 stands for the ultimate differential stress of UHPM rocks in the semi brittle flow field, and the average rheological stress (550-950 MPa) at the strain rate of l0 -14 - 10 -13 s -l stands for the ultimate differential stress of UHPM rocks in the plastic flow field, suggesting that the depth for the formation of UHPM rocks is more than 20-60 km below the depth estimated under static pressure condition due to the effect of the differential stress.

Dynamic Analysis of Deformational Structures of the Xianniishan Fault Zone in the Three Gorges of the Yangtze River

Field investigation and laboratory work reveal that inhomogeneity of the deformation of the Xiannushan fault is mainly characterized by lateral zonation, longitudinal segmentation and downward stratification. Based on these results, a 3-D deformational structure model of the fault was established and its geometrical and kinematic characteristics in two main deformational stages i.e. the main Yanshanian and Himalayan were discussed. The directions of principal and the differential stresses in these two stages were determined by using conjugate joints, striations of fault planes and microstructures of the fault zone. The direction of σI is N-S in direction with differential stresses of 150-250 MPa in the Yanshanian, and N70E with a differential stress ranging from 80-120 MPa in the Himalayan.

Quantitative reconstruction of formation paleo-pressure in sedimentary basins and case studies

Paleo-pressure reconstruction in sedimentary basins is one of the most important aspects of hydrocarbon accumulation research.In view of the advantages and disadvantages of the current methods for paleo-pressure research,a new method to reconstruct the paleo-pressure is presented in this paper.According to the geological background,quantitative analyses of the factors that might control overpressure were first conducted to clarify the contributions of each mechanism during different geological periods.Pressure evolution was reconstructed by fluid-compaction modelling with constraints imposed by the paleo-pressures obtained from fluid inclusions or differential stress methods.Determining the mechanisms responsible for overpressures during geological history is the basic prerequisite for paleo-pressure research.Thus,quantitative studies were conducted of the contributions of disequilibrium compaction,gas charging,oil cracking,temperature reduction,and tectonic uplift and subsidence to overpressures.Three case studies of paleo-pressure reconstruction were performed for the Sinian strata in the Sichuan Basin,Ordovician strata in the north uplift in the Tarim Basin and the Permian strata in the Sulige Gas Field in the Ordos Basin,where these three study sites are normally pressured,weakly over-pressured and abnormally low pressured at present,respectively.The new method developed in this paper is very important for the practical reconstruction of the paleopressure in marine strata and ancient strata in deep basins.

NUMERICAL SIMULATION AND EXPERIMENTAL INVESTIGATION OF THE GUIDE-VANE FLOW AND THE INFLUENCE FROM THE LEAKAGE FLOW

This paper presents numerical and experimental results of the flow in the tipclearance region of the guide vane row in hydraulic turbine. The 3-D Navier-Stokes equations wereemployed to model the flow in end clearance region of guide vane cascade, the Reynolds stressdifferential model was used for turbulence closure, and the body-fitted curvilinear coordinates andthe SIMPLE! algorithm were adopted. The governing equations were discretized with the non-staggeredgrids by means of the finite volume method. Detailed comparison of hydrodynamical characteristics ofguide vane in hydraulic turbine with or without tip gap was made. Special attention was paid to theinfluence of leakage flow on the main flow and to the movement of tip leakage flow in the end guidevane. The position and strength of the roll-vortex on the sides of guide vane in were determined.The numerical solutions agree with the experimental results obtained by particle image ve-locimetry.The results help to clarify the loss, wear and cavi-tation erosion between the guide vane andrings, especially for those used in the Yellow River which has a high sediment content.