The Plasmodtum falctparum DNA fragment was isolated from a cloned recombinant plasmid pPF14. labelled with Digoxigenin (Dig) and used as a probe (pPF14-F-Dig)to detect 274 blood samples from the eqdemic area populatio...The Plasmodtum falctparum DNA fragment was isolated from a cloned recombinant plasmid pPF14. labelled with Digoxigenin (Dig) and used as a probe (pPF14-F-Dig)to detect 274 blood samples from the eqdemic area population in Hainan Province by dot hybridization.The results showed that out of 274 blood specimens one was positive when using microscopic examination and the positivity rate was 0.36 percent. Fifteen samples showed to be positive (including one positive specimen examined by microscopy) when this probe was applied to detect the 274 samples and its positivity rate was 5.47 percent.The positive coincidence rate between pPF14-F-Dig and microscopic examination was 1/1 and the negative coincidence rate was 94.87 percent. Since a piece of nitrocellulose membrane with the size of 9 by 12 square centimetres can accommodate blots of 96 samples,this probe is fit for large-scale epidemiological surveys.展开更多
目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探...目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探针 ,所得探针有较高的敏感性 ;Dig- nor A基因探针安全、易操作 ,标记探针可长期保存。结论 为进一步研究 nor展开更多
文摘The Plasmodtum falctparum DNA fragment was isolated from a cloned recombinant plasmid pPF14. labelled with Digoxigenin (Dig) and used as a probe (pPF14-F-Dig)to detect 274 blood samples from the eqdemic area population in Hainan Province by dot hybridization.The results showed that out of 274 blood specimens one was positive when using microscopic examination and the positivity rate was 0.36 percent. Fifteen samples showed to be positive (including one positive specimen examined by microscopy) when this probe was applied to detect the 274 samples and its positivity rate was 5.47 percent.The positive coincidence rate between pPF14-F-Dig and microscopic examination was 1/1 and the negative coincidence rate was 94.87 percent. Since a piece of nitrocellulose membrane with the size of 9 by 12 square centimetres can accommodate blots of 96 samples,this probe is fit for large-scale epidemiological surveys.
文摘目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探针 ,所得探针有较高的敏感性 ;Dig- nor A基因探针安全、易操作 ,标记探针可长期保存。结论 为进一步研究 nor
