A LC-MS/MS method was established for the determination of streptomycin and dihydrostreptomycin in milk.The chromatographic analysis was performed on BEH C18 column of 50 mm×2.1 mm,1.7 μm with a mobile phase con...A LC-MS/MS method was established for the determination of streptomycin and dihydrostreptomycin in milk.The chromatographic analysis was performed on BEH C18 column of 50 mm×2.1 mm,1.7 μm with a mobile phase consisting of 20 mmol/L HFBA acetonitrile and 20 mmol/L HFBA as at flow rate of 0.3 mL/min under 30 ℃.Mass spectrometry was run under positive ESI and MRM mode.The calibration curve showed a good linearity between the peak areas and the concentrations from 0.05 to 2 mg/L with R2 more than 0.998.The of detection limit of the method was 2.5 μg/kg,and the limit of quantification was 5 μg/kg.The average recoveries from spiked milk at the three concentration levels of 50,100 and 200 μg/kg ranged from 83%-113%.The intra-and inter-batch variation coefficients were range of 1.8%-8.9% and 2.4%-12.2%,respectively.展开更多
文摘A LC-MS/MS method was established for the determination of streptomycin and dihydrostreptomycin in milk.The chromatographic analysis was performed on BEH C18 column of 50 mm×2.1 mm,1.7 μm with a mobile phase consisting of 20 mmol/L HFBA acetonitrile and 20 mmol/L HFBA as at flow rate of 0.3 mL/min under 30 ℃.Mass spectrometry was run under positive ESI and MRM mode.The calibration curve showed a good linearity between the peak areas and the concentrations from 0.05 to 2 mg/L with R2 more than 0.998.The of detection limit of the method was 2.5 μg/kg,and the limit of quantification was 5 μg/kg.The average recoveries from spiked milk at the three concentration levels of 50,100 and 200 μg/kg ranged from 83%-113%.The intra-and inter-batch variation coefficients were range of 1.8%-8.9% and 2.4%-12.2%,respectively.
