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缺氮条件对栅藻油脂积累与光合作用的影响 被引量:14
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作者 刘金丽 王俊峰 +1 位作者 刘天中 高莉丽 《海洋科学》 CAS CSCD 北大核心 2013年第7期13-19,共7页
栅通过油脂分析、光合放氧、叶绿素荧光等手段,研究了缺氮处理对栅藻(Scenedesmus dimorphus)油脂含量、油脂组分以及光合作用影响。结果显示,总脂含量由22.4%±0.6%提高到36.3%±0.7%,其中甘油三酯含量由4.6%±1.2%提高到6... 栅通过油脂分析、光合放氧、叶绿素荧光等手段,研究了缺氮处理对栅藻(Scenedesmus dimorphus)油脂含量、油脂组分以及光合作用影响。结果显示,总脂含量由22.4%±0.6%提高到36.3%±0.7%,其中甘油三酯含量由4.6%±1.2%提高到68.3%±2.5%而磷脂含量由92.8%±1.6%降低到26.8%±2.0%。缺氮处理1 d后,叶绿素含量明显下降,呼吸速率明显升高。缺氮处理初始阶段,栅藻光系统Ⅱ最大光能转化效率(Fv/Fm)和非光化学猝灭系数(NPQ)维持稳定,4 d后Fv/Fm显著降低而NPQ显著升高。上述变化可能是光能过剩引起的防御反应,也可能是自身代谢模式发生转变的结果而与光能耗散无关。 展开更多
关键词 栅藻(Scenedesmus dimorphus) 缺氮 油脂积累 光合作用
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Effect of nitrogen and extraction method on algae lipid yield
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作者 Ying Shen Zhijian Pei +1 位作者 Wenqiao Yuan Enrong Mao 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2009年第1期51-57,共7页
Effects of nitrogen source and concentration as well as lipid extraction method on the lipid yield of autotrophic Scenedesmus dimorphus and heterotrophic Chlorella protothecoides were studied.Three concentration level... Effects of nitrogen source and concentration as well as lipid extraction method on the lipid yield of autotrophic Scenedesmus dimorphus and heterotrophic Chlorella protothecoides were studied.Three concentration levels of nitrate,urea and glycine/yeast extract as the nitrogen source were investigated.The highest lipid yield of S.dimorphus in the 17-d autotrophic culture was 0.40 g/L from the 1.8 g/L urea medium,and the maximum lipid yield of C.protothecoides in the nine-day heterotrophic culture was 5.89 g/L from the 2.4 g/L nitrate medium.Four different cell disruption methods-bead-beater,French press,sonication and wet milling-were studied for their effectiveness in solvent extraction of algal lipids from S.dimorphus and C.protothecoides.Wet milling followed by hexane extraction was most effective for S.dimorphus lipid extraction,whereas bead-beater disruption followed by hexane extraction was best for C.protothecoides. 展开更多
关键词 ALGAE nitrogen source lipid content lipid extraction Scenedesmus dimorphus Chlorella protothecoides
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