Exploration of potential mechanisms for the treatment of ulcerative colitis by Solanum diphyllum L.based on network pharmacology and molecular docking

Evidence of the advantages of Solanum nigrum L.for the treatment of ulcerative colitis is accumulating.However,research revealing the treatment of Solanum diphyllum L.against ulcerative colitis is scarce.In this study,the chemical components of the extract of Solanum diphyllum L.were characterized by LC-MS/MS,identifying 31 compounds by positive and negative total ion flow maps.A total of 425 component target genes and 1900 disease target genes were obtained,and 121 intersection targets and 6 core targets were obtained after the intersection of the two genes by means of network pharmacology.GO analysis and KEGG analysis respectively obtained 20 signaling pathways such as anti-infl ammation.The results of molecular docking showed that the chemical components could successfully dock with the target proteins of the disease such as SRC,EGFR,PTGS2,MMP9,HSP90AA1,ESR1.This study provided a scientifi c basis for the development and application of Solanum diphyllum L.

黄果龙葵幼苗对镉胁迫的生理生长响应

黄果龙葵(Solanum diphyllum)是茄科的小灌木,已有的研究对黄果龙葵的镉(Cd)响应知之甚少。该研究采用水培控制试验,在160μmol·L^(-1)Cd胁迫下,测定了黄果龙葵幼苗在不同胁迫时间点(0、0.5、1、3、7、19 d)的生长、耐性系数(TI)、Cd富集特性、超氧化物歧化酶(SOD)活性、渗透调节物质和光合色素等指标。结果表明:(1)在胁迫0~3 d时Cd对黄果龙葵幼苗的生长无显著影响。胁迫3~19 d时表现为明显抑制,且毒害随胁迫时间延长而加重。与对照组相比,Cd胁迫使黄果龙葵幼苗的生物量降低13.28%~62.40%。(2)黄果龙葵幼苗的最大Cd积累量为60.14μg·plant^(-1),其地上部份的Cd积累量占植株的15.46%~35.24%。(3)丙二醛(MDA)含量呈上升趋势,根的MDA含量最大增幅为对照组的5.25倍。SOD活性、游离脯氨酸、可溶性糖、叶绿素a、叶绿素b和类胡萝卜素的含量均呈先升后降的趋势。(4)黄果龙葵幼苗的耐性系数TI随胁迫时间延长而降低,最小值为0.64。综上结果认为,黄果龙葵是一种潜在的Cd忍耐植物,其幼苗对高浓度的Cd胁迫在短时间内具有一定程度的耐性,并且黄果龙葵可能通过渗透调节和根系固持来缓解Cd毒害。该研究结果为寻找合适的植物修复材料提供了理论依据。