CoSe_(2) nanosheet materials with a wrinkled sheet structure and large surface area were synthesized via a simple hydrothermal method.Then polyethyleneimine(PEI)was used to functionalize wrinkled CoSe_(2)(w-CoSe_(2))t...CoSe_(2) nanosheet materials with a wrinkled sheet structure and large surface area were synthesized via a simple hydrothermal method.Then polyethyleneimine(PEI)was used to functionalize wrinkled CoSe_(2)(w-CoSe_(2))to make gold nanocubes(AuNCs)uniformly anchored onto the surface of PEI-w-CoSe_(2) via Au-N bonds.And its application in electrochemical immunosensors for the detection of dipropyl phthalate(DPrP)was studied.The obtained material was characterized by the Fourier transform infrared spectroscopy(FTIR),transmission electron microscope(TEM),energydispersive spectroscopy(EDS),X-ray diffraction(XRD)and Raman spectroscopy.The effective surface area and conductivity of the electrode were enhanced by the introduction of AuNCs/PEI-w-CoSe_(2) nanocomposite.Cyclic voltammetry(CV),differential pulse voltammetry(DPV)and electrochemical impedance spectroscopy(EIS)were used to characterize the performance of immunosensor.After DPrP binds to antibodies via the antigen-antibody interaction,[Fe(CN)6]3-/4-was used as a signal probe to monitor the change of oxidation current of different DPrP concentrations.Under optimal conditions,the electrochemical responses of the prepared immunosensor were linear when the DPrP concentrations ranged from 1×10^(-11) to 1×10^(-5) mol·L-1,with a detection limit of 1.39×10^(-12) mol·L^(-1).The coefficient of determination(R2)is 0.995,indicating that the degree of fitting is good.Besides,the constructed immunosensor exhibited acceptable reproducibility,selectivity,and stability.Therefore,it may be found that the composite material has good application prospects in the electrochemical sensors field.展开更多
基金financially supported by the National Natural Science Foundation of China(No.61301037)the Program for Science and Technology Innovation Talents in Universities of Henan Province(No.20HASTIT002)+1 种基金the Cultivation Plan for Young Core Teachers in Universities of Henan Province(No.2017GGJS072)the National Engineering Laboratory for Wheat&Corn Further Processing,Henan University of Technology(No.NL2018004)。
文摘CoSe_(2) nanosheet materials with a wrinkled sheet structure and large surface area were synthesized via a simple hydrothermal method.Then polyethyleneimine(PEI)was used to functionalize wrinkled CoSe_(2)(w-CoSe_(2))to make gold nanocubes(AuNCs)uniformly anchored onto the surface of PEI-w-CoSe_(2) via Au-N bonds.And its application in electrochemical immunosensors for the detection of dipropyl phthalate(DPrP)was studied.The obtained material was characterized by the Fourier transform infrared spectroscopy(FTIR),transmission electron microscope(TEM),energydispersive spectroscopy(EDS),X-ray diffraction(XRD)and Raman spectroscopy.The effective surface area and conductivity of the electrode were enhanced by the introduction of AuNCs/PEI-w-CoSe_(2) nanocomposite.Cyclic voltammetry(CV),differential pulse voltammetry(DPV)and electrochemical impedance spectroscopy(EIS)were used to characterize the performance of immunosensor.After DPrP binds to antibodies via the antigen-antibody interaction,[Fe(CN)6]3-/4-was used as a signal probe to monitor the change of oxidation current of different DPrP concentrations.Under optimal conditions,the electrochemical responses of the prepared immunosensor were linear when the DPrP concentrations ranged from 1×10^(-11) to 1×10^(-5) mol·L-1,with a detection limit of 1.39×10^(-12) mol·L^(-1).The coefficient of determination(R2)is 0.995,indicating that the degree of fitting is good.Besides,the constructed immunosensor exhibited acceptable reproducibility,selectivity,and stability.Therefore,it may be found that the composite material has good application prospects in the electrochemical sensors field.
