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Laser-direct-drive program: Promise, challenge, and path forward 被引量:12
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作者 E.M.Campbell V.N.Goncharov +33 位作者 T.C.Sangster S.P.Regan P.B.Radha R.Betti J.F.Myatt D.H.Froula M.J.Rosenberg I.V.Igumenshchev W.Seka A.A.Solodov A.V.Maximov J.A.Marozas T.J.B.Collins D.Turnbull F.J.Marshall A.Shvydky J.P.Knauer R.L.McCrory A.B.Sefkow M.Hohenberger P.A.Michel T.Chapman L.Masse C.Goyon S.Ross J.W.Bates M. Karasik J.Oh J.Weaver A.J.Schmitt K.Obenschain S.P.Obenschain S.Reyes B.Van Wonterghem 《Matter and Radiation at Extremes》 SCIE EI CAS 2017年第2期37-54,共18页
Along with laser-indirect(X-ray)-drive and magnetic-drive target concepts,laser direct drive is a viable approach to achieving ignition and gain with inertial confinement fusion.In the United States,a national program... Along with laser-indirect(X-ray)-drive and magnetic-drive target concepts,laser direct drive is a viable approach to achieving ignition and gain with inertial confinement fusion.In the United States,a national program has been established to demonstrate and understand the physics of laser direct drive.The program utilizes the Omega Laser Facility to conduct implosion and coupling physics at the nominally 30-kJ scale and lasereplasma interaction and coupling physics at the MJ scale at the National Ignition Facility.This article will discuss the motivation and challenges for laser direct drive and the broad-based program presently underway in the United States. 展开更多
关键词 Inertial fusion direct drive Laser interactions HYDRODYNAMICS OMEGA National ignition facility
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DIRECT DIGITAL DESIGN AND SIMULATION OF MESHING IN WORM-GEAR DRIVE 被引量:10
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作者 XU Wujiao QIN Datong SHI Wankai 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2006年第3期428-433,共6页
A direct digital design method (DDDM) of worm-gear drive is proposed. It is directly based on the simulation of manufacturing process and completely different from the conventional modeling method. The loaded tooth ... A direct digital design method (DDDM) of worm-gear drive is proposed. It is directly based on the simulation of manufacturing process and completely different from the conventional modeling method. The loaded tooth contact analysis (LTCA) method is analyzed, in which the advanced surface to surface searching technique is included. The influence of misalignment errors and contact deformations on contact zone and transmission error (TE) is discussed. Combined modification approach on worm tooth surface is presented. By means of DDDM and LTCA, it is very conven- ient to verify the effect of worm-gear drive's modification approach. The analysis results show that, the modification in profile direction reduces the sensitivity of worm-gear drive to misalignment errors and the modification in longitudinal direction decreases the TE. Thus the optimization design of worm-gear drive can be achieved prior to the actual manufacturing process. 展开更多
关键词 direct digital design Loaded tooth contact analysis Combined modification approach Worm-gear drive
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Submersible direct-drive progressing cavity pump rodless lifting technology 被引量:3
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作者 HAO Zhongxian ZHU Shijia +4 位作者 PEI Xiaohan HUANG Peng TONG Zheng WANG Benyuan LI Deyin 《Petroleum Exploration and Development》 2019年第3期621-628,共8页
Submersible electrical motor direct-drive progressing cavity pump (PCP) rodless lifting was studied to solve the traditional rod-drive pump problems, such as rod-tubing wearing, low efficiency and short running time. ... Submersible electrical motor direct-drive progressing cavity pump (PCP) rodless lifting was studied to solve the traditional rod-drive pump problems, such as rod-tubing wearing, low efficiency and short running time. The theoretical researches and laboratory experiments of key tools such as submersible motor and the construction technology of lifting system were introduced. The field application and economic benefit were analyzed and compared with the traditional rod pumping unit. A new low speed and large torque permanent magnet synchronous motor was developed. This motor was used to drive PCP without gear reducer, which improved the reliability and feasibility. It can run at the speed from 50 to 500 r/min with stepless speed regulation, and it can perform high efficiency and large torque. Besides, other key supporting tools, such as motor protector and flex shaft, were developed. The submersible electrical motor direct- drive PCP technology can be used in a 139.7 mm (5.5 in) casing well, with daily output ranging from 5 to 50 m3. Until now, the technology has been deployed more than 100 wells. The field application results show that it eliminates the rod-tubing wearing and saves electric energy by more than 30% compared with the traditional rod pumping unit. And it also makes the oil produced in a safe and environmental friendly way. 展开更多
关键词 rodless artificial LIFT SUBMERSIBLE MOTOR direct-drive progressing CAVITY PUMP SUBMERSIBLE MOTOR artificial LIFT
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Analysis of nonlinearities and effects in direct drive electro-hydraulic position servo system 被引量:2
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作者 王洪杰 季天晶 +1 位作者 毛新涛 刘全忠 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2005年第1期6-10,共5页
The direct drive electro-hydraulic servo system is a new approach hydraulic system. It is much smaller and easier controlled than traditional systems and is a perfect energy saver. This paper will briefly introduce th... The direct drive electro-hydraulic servo system is a new approach hydraulic system. It is much smaller and easier controlled than traditional systems and is a perfect energy saver. This paper will briefly introduce the popular nonlinearities in the electro-hydraulic system and analyse the effect of nonlinearities in direct drive electro-hydraulic position servo system by means of simulation research. Some valuable conclusions are given. 展开更多
关键词 direct drive electro-hydraulic system NONLINEARITIES EFFECT
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Research on Direct Yaw Moment Control Strategy of Distributed-Drive Electric Vehicle Based on Joint Observer 被引量:1
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作者 Quan Min Min Deng +3 位作者 Zichen Zheng Shu Wang Xianyong Gui Haichuan Zhang 《Energy Engineering》 EI 2021年第4期853-874,共22页
Combined with the characteristics of the distributed-drive electric vehicle and direct yaw moment control,a double-layer structure direct yaw moment controller is designed.The upper additional yaw moment controller is... Combined with the characteristics of the distributed-drive electric vehicle and direct yaw moment control,a double-layer structure direct yaw moment controller is designed.The upper additional yaw moment controller is constructed based on model predictive control.Aiming at minimizing the utilization rate of tire adhesion and constrained by the working characteristics of motor system and brake system,a quadratic programming active set was designed to optimize the distribution of additional yaw moments.The road surface adhesion coefficient has a great impact on the reliability of direct yaw moment control,for which joint observer of vehicle state parameters and road surface parameters is designed by using unscented Kalman filter algorithm,which correlates vehicle state observer and road surface parameter observer to form closed-loop feedback correction.The results show that compared to the“feedforward+feedback”control,the vehicle’s error of yaw rate and sideslip angle by the model predictive control is smaller,which can improve the vehicle stability effectively.In addition,according to the results of the docking road simulation test,the joint observer of vehicle state and road surface parameters can improve the adaptability of the vehicle stability controller to the road conditions with variable adhesion coefficients. 展开更多
关键词 Vehicle stability control distributed drive direct yaw moment control joint observer
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First polar direct-drive exploding-pusher target experiments on the ShenGuang laser facility
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作者 Bo Yu Jiamin Yang +20 位作者 Tianxuan Huang Peng Wang Wanli Shang Xiumei Qiao Xuewei Deng Zhanwen Zhang Zifeng Song Qi Tang Xiaoshi Peng Jiabin Chen Yulong Li Wei Jiang Yudong Pu Ji Yan Zhongjing Chen Yunsong Dong Wudi Zheng Feng Wang Shaoen Jiang Yongkun Ding Jian Zheng 《Chinese Physics B》 SCIE EI CAS CSCD 2019年第9期288-294,共7页
Low density and low convergence implosion occurs in the exploding-pusher target experiment, and generates neutrons isotropically to develop a high yield platform.In order to validate the performance of ShenGuang(SG) l... Low density and low convergence implosion occurs in the exploding-pusher target experiment, and generates neutrons isotropically to develop a high yield platform.In order to validate the performance of ShenGuang(SG) laser facility and test nuclear diagnostics, all 48-beam lasers with an on-target energy of 48 kJ were firstly used to drive room-temperature, DT gas-filled glass targets.The optimization has been carried out and optimal drive uniformity was obtained by the combination of beam repointing and target.The final irradiation uniformity of less than 5% on polar direct-drive capsules of 540 μm in diameter was achieved, and the highest thermonuclear yield of the polar direct-drive DT fuel implosion at the SG was 1.04 × 10^(13).The experiment results show neutron yields severely depend on the irradiation uniformity and laser timing,and decrease with the increase of the diameter and fuel pressure of the target.The thin CH ablator does not impact the implosion performance, but the laser drive uniformity is important.The simulated results validate that the cos γ distribution laser design is reasonable and can achieve a symmetric pressure distribution.Further optimization will focus on measuring the symmetry of the hot spot by self-emission imaging, increasing the diameter, and decreasing the fuel pressure. 展开更多
关键词 POLAR direct-drive exploding-pusher TARGET neutron yield IRRADIATION UNIFORMITY
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A simple analytical model of laser direct-drive thin shell target implosion
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作者 Bo Yu Tianxuan Huang +14 位作者 Li Yao Chuankui Sun Wanli Shang Peng Wang Xiaoshi Peng Qi Tang Zifeng Song Wei Jiang Zhongjing Chen Yudong Pu Ji Yan Yunsong Dong Jiamin Yang Yongkun Ding Jian Zheng 《Chinese Physics B》 SCIE EI CAS CSCD 2022年第4期481-485,共5页
A high-neutron yield platform imploded by a thin shell target is generally built to probe nuclear science problems,and it has the advantages of high neutron yield,ultrashort fusion time,micro fusion zone,isotropic and... A high-neutron yield platform imploded by a thin shell target is generally built to probe nuclear science problems,and it has the advantages of high neutron yield,ultrashort fusion time,micro fusion zone,isotropic and monoenergetic neutron.Some analytical models have been proposed to interpret exploding-pusher target implosion driven by a long wavelength laser,whereas they are imperfect for a 0.35 μm laser implosion experiment.When using the 0.35 μm laser,the shell is ablated and accelerated to high implosion velocity governed by Newton’s law,ablation acceleration and quasi-adiabatic compression models are suitable to explain the implosion of a laser direct-drive thin shell target.The new analytical model scales bang time,ion temperature and neutron yield for large variations in laser power,target radius,shell thickness,and fuel pressure.The predicted results of the analytical model are in agreement with experimental data on the ShenguangIII prototype laser facility,100 kJ laser facility,Omega,and NIF,it demonstrates that the analytical model benefits the understanding of experiment performance and optimizing the target design of high neutron yield implosion. 展开更多
关键词 direct drive analytical model thin shell target neutron yield
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Research on Bus Capacitor Current Control Scheme for Buck/Boost Bi-directional Converters in Motor Drive Systems
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作者 ZHANG Xuhui WEN Xuhui ZHAO Feng 《中国电机工程学报》 EI CSCD 北大核心 2012年第30期I0004-I0004,共1页
关键词 电机驱动系统 升压转换器 控制计划 电容电流 降压 总线 母线电压 控制方案
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匹配控制构网型直驱风电场经LCC-HVDC 送出 系统的次同步振荡特性及机理分析
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作者 高本锋 董涵枭 +3 位作者 孙大卫 吴林林 王潇 邓晓洋 《中国电机工程学报》 EI CSCD 北大核心 2024年第23期9296-9309,I0016,共15页
在“沙戈荒”地区风电经电网换相高压直流输电(line-commutated-converter based high voltage direct current,LCC-HVDC)外送系统中,采用基于匹配控制的构网型直驱风机(matching control permanent magnet synchronous generator,MC-PM... 在“沙戈荒”地区风电经电网换相高压直流输电(line-commutated-converter based high voltage direct current,LCC-HVDC)外送系统中,采用基于匹配控制的构网型直驱风机(matching control permanent magnet synchronous generator,MC-PMSG)可以提升送端电网的稳定性。然而,当MC-PMSG位于LCC-HVDC整流站近区时,系统的次同步振荡(sub-synchronous oscillation,SSO)特性尚未明确。针对上述问题,该文采用模块化建模法建立MC-PMSG经LCC-HVDC送出系统的小信号模型,通过特征值法研究MC-PMSG与LCC-HVDC对系统各SSO模态的参与情况与系统运行方式变化对次同步振荡阻尼特性的影响,通过阻尼重构法分析LCC-HVDC并网对系统振荡风险的影响机理。研究结果表明,系统存在匹配控制型风机主导、LCC-HVDC参与的SSO模态,MC-PMSG与LCC-HVDC间的次同步交互作用为SSO提供负阻尼;当混合型风电场中的MC-PMSG占比增大、MC-PMSG风电场容量增大或短路比减小、LCC-HVDC定电流控制器的比例系数增大、风机网侧换流控制器外环积分系数减小、直流电容增大时,SSO阻尼增大。通过PSCAD/EMTDC电磁暂态仿真证明理论分析结果的有效性。 展开更多
关键词 电网换相高压直流输电 构网型直驱风机 匹配控制 次同步振荡 特征值分析 阻尼重构
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考虑频率耦合特性的D-PMSG复数域阻抗建模及其特征分析
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作者 苏晨博 刘崇茹 +2 位作者 王瑾媛 郝琪 董浩云 《电力系统自动化》 EI CSCD 北大核心 2024年第10期118-128,共11页
阻抗分析法因其简单有效被广泛用于含电力电子变换器的互联系统稳定性研究。然而,采用传统方法对频域序阻抗建模时,需通过非线性变换(Park变换及其反变换)进行坐标系转换,这会导致不同周期分量的卷积运算及相应的频移,其过程较为烦琐。... 阻抗分析法因其简单有效被广泛用于含电力电子变换器的互联系统稳定性研究。然而,采用传统方法对频域序阻抗建模时,需通过非线性变换(Park变换及其反变换)进行坐标系转换,这会导致不同周期分量的卷积运算及相应的频移,其过程较为烦琐。针对上述问题,基于复向量时域微分算子,提出了永磁直驱同步发电机(D-PMSG)等效阻抗建模方法。该方法在时域周期系统稳定运行轨迹的邻域内直接进行小信号线性化,并采用指数旋转因子灵活反映系统受扰后的时变特性,结合时域微分算子等效表征互联系统控制环节的动态特性,避免了频域阻抗建模时坐标系的反复变换,大幅降低了解析建模的复杂度。此外,所提方法能够更加清晰地阐释互联系统受到扰动时D-PMSG并网逆变器产生频率耦合现象的物理意义;同时,能够说明基频电压初始相位对耦合分量相频特性的影响。最后,利用仿真验证了所提算法的有效性。 展开更多
关键词 复向量 阻抗建模 永磁直驱同步发电机(D-PMSG) 频率耦合 系统稳定性分析
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基于Wi-Fi Direct的道路交通状态信息采集方法
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作者 李珺 《计算机测量与控制》 2015年第12期4172-4176,共5页
针对传统交通状态信息采集中采用的环形线圈车辆检测器存在布设和维护难度较大等问题,提出了一种基于Wi-Fi Direct的道路交通状态信息采集方法,以实现对基本的道路交通流状态参数进行采集估计;该方法利用车-路通讯设备实现车辆与路侧设... 针对传统交通状态信息采集中采用的环形线圈车辆检测器存在布设和维护难度较大等问题,提出了一种基于Wi-Fi Direct的道路交通状态信息采集方法,以实现对基本的道路交通流状态参数进行采集估计;该方法利用车-路通讯设备实现车辆与路侧设备的通信;以车载通讯设备的Wi-Fi芯片介质访问控制层地址完成车辆个体识别;通过车载通讯设备中的北斗定位装置向路侧设备提供车辆实时位置及时间信息,进而实现道路区段内交通流基本状态信息估计;实验测试表明,该方法能够完成路段平均速度、交通流量及车流密度的采集和估计,是一种有效的道路交通信息采集方法。 展开更多
关键词 交通状态信息 -路通讯 交通流 WI-FI direct 介质访问控制层地址
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Modeling and Analysis of A Rotary Direct Drive Servovalve 被引量:12
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作者 YU Jue ZHUANG Jian YU Dehong 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2014年第5期1064-1074,共11页
Direct drive servovalves are mostly restricted to low flow rate and low bandwidth applications due to the considerable flow forces.Current studies mainly focus on enhancing the driving force,which in turn is limited t... Direct drive servovalves are mostly restricted to low flow rate and low bandwidth applications due to the considerable flow forces.Current studies mainly focus on enhancing the driving force,which in turn is limited to the development of the magnetic material.Aiming at reducing the flow forces,a novel rotary direct drive servovalve(RDDV)is introduced in this paper.This RDDV servovalve is designed in a rotating structure and its axially symmetric spool rotates within a certain angle range in the valve chamber.The servovalve orifices are formed by the matching between the square wave shaped land on the spool and the rectangular ports on the sleeve.In order to study the RDDV servovalve performance,flow rate model and mechanical model are established,wherein flow rates and flow induced torques at different spool rotation angles or spool radiuses are obtained.The model analysis shows that the driving torque can be alleviated due to the proposed valve structure.Computational fluid dynamics(CFD)analysis using ANSYS/FLUENT is applied to evaluate and validate the theoretical analysis.In addition,experiments on the flow rate and the mechanical characteristic of the RDDV servovalve are carried out.Both simulation and experimental results conform to the results of the theoretical model analysis,which proves that this novel and innovative structure for direct drive servovalves can reduce the flow force on the spool and improve valve frequency response characteristics.This research proposes a novel rotary direct drive servovalve,which can reduce the flow forces effectively. 展开更多
关键词 direct drive servovalve computational fluid dynamics(CFD) flow rate flow induced torque
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直驱风电场经MMC-HVDC并网送端系统稳定性分析及振荡抑制 被引量:3
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作者 曾瑞 雷鸣 +1 位作者 王一振 何晋伟 《电力系统及其自动化学报》 CSCD 北大核心 2024年第2期26-35,共10页
基于模块化多电平换流器MMC(modular multilevel converter)的高压直流输电HVDC(high voltage direct current transmission)因具有无源网络支撑等优势而被广泛应用于大容量新能源外送消纳。受电力电子设备交互作用等因素影响,送端系统... 基于模块化多电平换流器MMC(modular multilevel converter)的高压直流输电HVDC(high voltage direct current transmission)因具有无源网络支撑等优势而被广泛应用于大容量新能源外送消纳。受电力电子设备交互作用等因素影响,送端系统易发生振荡失稳现象。首先,建立了直驱风电场经MMC-HVDC并网送端系统的小扰动线性化模型,分析了风场有功输出对系统稳定性的影响。然后,建立了MMC及风机并网变流器交流侧dq阻抗模型,从阻抗角度揭示了送端系统振荡失稳机理。进一步,提出了基于MMC交流电压控制外环q轴附加阻尼的振荡抑制策略,可满足系统满功率范围内的运行稳定性要求。最后,基于全比例模型的仿真结果验证了所提振荡抑制策略的有效性。 展开更多
关键词 直驱风场 MMC-HVDC 稳定性分析 振荡抑制
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基于改进PSO-LSTM算法的风电机组状态监测方法研究
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作者 王印松 刘佳微 +1 位作者 贾思宇 翁疆 《山东电力技术》 2024年第5期30-37,共8页
通过改进粒子群算法(particle swarm optimization,PSO)优化长短期记忆神经网络算法(long short-term memory,LSTM)的参数,提出了一种基于改进PSO-LSTM算法的直驱式风电机组运行状态监测方法。首先将数据采集与监控系统(supervisory con... 通过改进粒子群算法(particle swarm optimization,PSO)优化长短期记忆神经网络算法(long short-term memory,LSTM)的参数,提出了一种基于改进PSO-LSTM算法的直驱式风电机组运行状态监测方法。首先将数据采集与监控系统(supervisory control and data acquisition,SCADA)采集到的数据利用随机森林的方法进行特征筛选,得到模型的输入参数;其次采用改进PSO-LSTM网络建立有功功率的预测模型,计算出预测值与实际值的残差,根据残差的分布来确实直驱式风电机组的状态;最后利用某风电机组SCADA数据对所提预测模型进行验证分析,结果表明,PSO-LSTM预测模型相比其他三种预测模型,具有较高的预测精度,并在状态异常后最短时间内发出故障警报,保证电场的健康稳定运行。 展开更多
关键词 直驱式风力发电机 状态监测 粒子群算法 长短期记忆网络
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Direct Yaw Moment Control for Distributed Drive Electric Vehicle Handling Performance Improvement 被引量:30
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作者 YU Zhuoping LENG Bo +2 位作者 XIONG Lu FENG Yuan SHI Fenmiao 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2016年第3期486-497,共12页
For a distributed drive electric vehicle(DDEV) driven by four in-wheel motors, advanced vehicle dynamic control methods can be realized easily because motors can be controlled independently, quickly and precisely. A... For a distributed drive electric vehicle(DDEV) driven by four in-wheel motors, advanced vehicle dynamic control methods can be realized easily because motors can be controlled independently, quickly and precisely. And direct yaw-moment control(DYC) has been widely studied and applied to vehicle stability control. Good vehicle handling performance: quick yaw rate transient response, small overshoot, high steady yaw rate gain, etc, is required by drivers under normal conditions, which is less concerned, however. Based on the hierarchical control methodology, a novel control system using direct yaw moment control for improving handling performance of a distributed drive electric vehicle especially under normal driving conditions has been proposed. The upper-loop control system consists of two parts: a state feedback controller, which aims to realize the ideal transient response of yaw rate, with a vehicle sideslip angle observer; and a steering wheel angle feedforward controller designed to achieve a desired yaw rate steady gain. Under the restriction of the effect of poles and zeros in the closed-loop transfer function on the system response and the capacity of in-wheel motors, the integrated time and absolute error(ITAE) function is utilized as the cost function in the optimal control to calculate the ideal eigen frequency and damper coefficient of the system and obtain optimal feedback matrix and feedforward matrix. Simulations and experiments with a DDEV under multiple maneuvers are carried out and show the effectiveness of the proposed method: yaw rate rising time is reduced, steady yaw rate gain is increased, vehicle steering characteristic is close to neutral steer and drivers burdens are also reduced. The control system improves vehicle handling performance under normal conditions in both transient and steady response. State feedback control instead of model following control is introduced in the control system so that the sense of control intervention to drivers is relieved. 展开更多
关键词 direct yaw moment control distributed drive electric vehicle handling performance improvement state feedback control
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Optimization of laser illumination configuration for directly driven inertial confinement fusion 被引量:2
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作者 Masakatsu Murakami Daiki Nishi 《Matter and Radiation at Extremes》 SCIE EI CAS 2017年第2期55-68,共14页
Optimum laser configurations are presented to achieve high illumination uniformity with directly driven inertial confinement fusion targets.Assuming axisymmetric absorption pattern of individual laser beams,theoretica... Optimum laser configurations are presented to achieve high illumination uniformity with directly driven inertial confinement fusion targets.Assuming axisymmetric absorption pattern of individual laser beams,theoretical models are reviewed in terms of the number of laser beams,system imperfection,and laser beam patterns.Utilizing a self-organizing system of charged particles on a sphere,a simple numerical model is provided to give an optimal configuration for an arbitrary number of laser beams.As a result,such new configurations as“M48”and“M60”are found to show substantially higher illumination uniformity than any other existing direct drive systems.A new polar direct-drive scheme is proposed with the laser axes keeping off the target center,which can be applied to laser configurations designed for indirectly driven inertial fusion. 展开更多
关键词 Analytical model Laser illumination design Polar direct drive Inertial confinement fusion
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Enhancement of the thermostability of β-1,3-1,4-glucanase by directed evolution 被引量:2
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作者 ZHANG Xiu-yan RUAN Hui +3 位作者 MU Lin HE Guo-qing TANG Xing-jun CHEN Qi-he 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2006年第11期1948-1955,共8页
In order to improve the thermostability of β- 1,3-1,4-glucanase, evolutionary molecular engineering was used to evolve the β-1,3-1,4-glucanase from Bacillus subtilis ZJF-1A5. The process involves random mutation by ... In order to improve the thermostability of β- 1,3-1,4-glucanase, evolutionary molecular engineering was used to evolve the β-1,3-1,4-glucanase from Bacillus subtilis ZJF-1A5. The process involves random mutation by error-prone PCR and DNA shuffling followed by screening on the filter-based assay. Two mutants, EGsl and EGs2, were found to have four and five amino acid substitutions, respectively. These substitutions resulted in an increase in melting temperature from Tm=62.5℃ for the wild-type enzyme to Tm=65.5℃ for the mutant EGsl and 67.5℃ for the mutant EGs2. However, the two mutated enzymes had opposite approaches to produce reducing sugar from lichenin with either much higher (28%) for the former or much lower (21.6%) for the latter in comparison with their parental enzymes. The results demonstrate that directed evolution is an effective approach to improve the thermostability of a mesophilic enzyme. 展开更多
关键词 directed evolution Error-prone PCR DNA shuffling β- 1 3-1 4-glucanase Thermostability
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Effects on coagulation factor production following primary hepatomitogen-induced direct hyperplasia 被引量:5
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作者 Kohei Tatsumi Kazuo Ohashi +5 位作者 Sanae Taminishi Soichi Takagi Rie Utoh Akira Yoshioka Midori Shima Teruo Okano 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第42期5307-5315,共9页
AIM: To investigate the molecular mechanisms involved in coagulation factor expression and/or function during direct hyperplasia (DH)-mediated liver regeneration. METHODS: Direct hyperplasia-mediated liver regener... AIM: To investigate the molecular mechanisms involved in coagulation factor expression and/or function during direct hyperplasia (DH)-mediated liver regeneration. METHODS: Direct hyperplasia-mediated liver regeneration was induced in female C57BL/6 mice by administering 1,4-bisr2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP), a representative hepatomitogen. Mice were weighed and sacrificed at various time points [Day 0 (D0: prior to injection), 3 h, D1, D2, D3, and D10] after TCPOBOP administration to obtain liver and blood samples. Using the RNA samples extracted from the liver, a comprehensive analysis was performed on the hepatic gene expression profiling of coagulation-related factors by real-time RT-PCR (fibrinogen, prothrombin, factors Ⅴ, Ⅶ, Ⅷ, Ⅸ, Ⅹ, Ⅺ, Ⅻ, ⅩⅢβ , plasminogen, antithrombin, protein C, protein S, ADAMTS13, and VWF). The corresponding plasma levels of coagulation factors (fibrinogen, prothrombin, factors Ⅴ, Ⅶ, Ⅷ, Ⅸ, Ⅹ, Ⅺ, Ⅻ, ⅩⅢ, and VWF) were also analyzed and compared with their mRNA levels. RESULTS: Gavage administration of TCPOBOP (3 mg/kg body weight) resulted in a marked and gradual increase in the weight of the mouse livers relative to the total body weight to 220% by D10 relative to the DO (control) ratios. At the peak of liver regeneration (D1 and D2), the gene expression levels for most of the coagulationrelated factors (fibrinogen, prothrombin, factors Ⅴ, Ⅶ, Ⅷ, Ⅸ,Ⅺ, Ⅻ, ⅩⅢβ, plasminogen, antithrombin, protein C, ADAMTS13, VWF) were found to be downregulated in a time-dependent manner, and gradually recovered by D10 to the basal levels. Only mRNA levels of factor X and protein S failed to show any decrease during the regenerative phase. As for the plasma levels, 5 clotting factors (prothrombin, factors Ⅷ, Ⅸ, Ⅺ, and Ⅻ) demonstrated a significant decrease (P 〈 0.05) during the regeneration phase compared with DO. Among these 5 factors, factor Ⅸ and factor Ⅺ showed the most dramatic decline in their activities by about 50% at D2 compared to the basal levels, and these reductions in plasma activity for both factors were consistent with our RT-PCR findings. In contrast, the plasma activities of the other coagulation factors (fibrinogen, factors Ⅴ, Ⅶ, ⅩⅢ, and VWF) were not significantly reduced, despite the reduction in the liver mRNA levels. Unlike the other factors, FX showed a temporal increase in its plasma activity, with significant increases (P 〈 0.05) detected at DI. CONCLUSION: Investigating the coagulation cascade protein profiles during liver regeneration by DH may help to better understand the basic biology of the liver under normal and pathological conditions. 展开更多
关键词 Coagulation factor 1 4-bis[2-(3 5-dichlo- ropyridyloxy)] benzene direct hyperplasia Liver regeneration
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High Performance Servo-System of a Motor in Driect Drive Robotics
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作者 Feng Yong Wang Yan Hu Hengzhang (Department of Electrical Engineering) 《哈尔滨工业大学学报》 EI CAS CSCD 北大核心 1990年第3期99-104,共6页
This paper decribes the control of a high performance variable reluctance motor system for direct drive robotics and industrial automation. The control system of a motor comists of a drive unit and a digital controlle... This paper decribes the control of a high performance variable reluctance motor system for direct drive robotics and industrial automation. The control system of a motor comists of a drive unit and a digital controller, possessing two functions of tbe analog dosed-loop control of motor velocity and the digit dosed-loop control of motor position. Then it discusses the closed-loop control of current in the three phases of the motor and the control of the lead angle of the motor. Finally, it suggests a design of the control circuits of motor current, velocity and position. The closed loop control of the motor position is achieved by a digit cotroller which consists of a microprocessor and other electronic components. It can control two variable reluctance motors simultaneusly. In order to be used for directly driving robots, the digit cotroller is equipped with a universal interface. 展开更多
关键词 直接驱动机器人 转矩控制 速度控制 位置控制 变磁阻电动机 伺服系统
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La_(0.75)Sr_(0.25)Cr_(0.5)Mn_(0.5)O_(3)-δ−Ce_(0.8)Gd_(0.2)O_(1.9) composite electrodes as anodes in LaGaO_(3)-based direct carbon solid oxide fuel cells 被引量:2
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作者 CHEN Tian-yu XIE Yong-min +7 位作者 LU Zhi-bin WANG Liang CHEN Zhe-qin ZHONG Xiao-cong LIU Jia-ming WANG Rui-xiang XU Zhi-feng OUYANG Shao-bo 《Journal of Central South University》 SCIE EI CAS CSCD 2022年第6期1788-1798,共11页
Direct carbon solid oxide fuel cells(DC-SOFCs)are promising,green,and efficient power-generating devices that are fueled by solid carbons and comprise all-solid-state structures.Developing suitable anode materials for... Direct carbon solid oxide fuel cells(DC-SOFCs)are promising,green,and efficient power-generating devices that are fueled by solid carbons and comprise all-solid-state structures.Developing suitable anode materials for DC-SOFCs is a substantial scientific challenge.Herein we investigated the use of La_(0.75)Sr_(0.25)Cr_(0.5)Mn_(0.5)O_(3)-δ−Ce_(0.8)Gd_(0.2)O_(1.9)(LSCM−GDC)composite electrodes as anodes for La_(0.9)Sr_(0.1)Ga_(0.8)Mg_(0.2)O_(3)-δelectrolyte-based DC-SOFCs,with Camellia oleifera shell char as the carbon fuel.The LSCM−GDC-anode DC-SOFC delivered a maximum power density of 221 mW/cm^(2) at 800℃ and it significantly improved to 425 mW/cm^(2) after Ni nanoparticles were introduced into the LSCM−GDC anode through wet impregnation.The microstructures of the prepared anodes were characterized,and the stability of the anode in a DC-SOFC and the influence of catalytic activity on open circuit voltage were studied.The above results indicate that LSCM–GDC anode is promising to be applied in DC-SOFCs. 展开更多
关键词 direct carbon solid oxide fuel cells anode material La_(0.75)Sr_(0.25)Cr_(0.5)Mn_(0.5)O_(3)-δ−Ce_(0.8)Gd_(0.2)O_(1.9) composite electrodes Ni nanoparticles
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