Access to security and safe food is a basic human necessity and essential for a sustainable world. To perform hi-end rood safety analysis and risk assessment with state of the art technologies is of utmost importance ...Access to security and safe food is a basic human necessity and essential for a sustainable world. To perform hi-end rood safety analysis and risk assessment with state of the art technologies is of utmost importance thereof. With applications as exemplified by microfiuidic immunoassay, aptasensor, direct analysis in real time, high resolution mass spectrometry, benchmark dose and chemical specific adjustment factor, this review presents frontier food safety analysis and risk assessment technologies, from which both food quality and public health will benefit undoubtedly in a foreseeable future.展开更多
A method for rapid identification and quantification of phthalate plasticizers in beverages was developed.A number of 15 phthalate plasticizers which covered all the phthalates concerned in the US Consumer Product Saf...A method for rapid identification and quantification of phthalate plasticizers in beverages was developed.A number of 15 phthalate plasticizers which covered all the phthalates concerned in the US Consumer Product Safety Improvement Act(CPSIA),European Union legislations and Chinese national standards(GB)were analyzed.By a combined solid-phase micro-extraction(SPME)and direct analysis in real time mass spectrometry(DART-MS)approach,phthalates at sub-ng•mL^(−1)levels can be qualitatively and quantitatively analyzed in a short time.The use of ultrahigh-resolving power and the accurate mass measurement capacity naturally provided by Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS)minimizes the matrix interferences and thus enables the evaluation of phthalates in a complex matrix without extensive sample handlings or preparations.The limits of quantification(LOQs)were estimated to be at 0.3-5.0 ng•mL^(−1),lower than the Maximum Residue Limit(MRL)regulated by the European Union legislations(2007/19/EC)in foods,beverages,food packaging and toys(0.3-30 ng•mL^(−1)).This rapid and easy-to-use SPME-DART-FT-ICR-MS method provided a relatively high-throughput and powerful analytical approach for quick testing and screening phthalates in beverages and water samples to ensure food safety.展开更多
We aimed to establish for the rapid detection of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in human blood and urine by direct analysis in real‑time coupled w...We aimed to establish for the rapid detection of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in human blood and urine by direct analysis in real‑time coupled with tandem mass spectrometry(DART‑MS/MS).These samples were extracted by acetonitrile‑methanol(V/V=4:1),using DART 12 Dip‑it automatic sampling system.They were injected at 400℃,and analyzed by positive ion and multiple reaction monitoring mode.The detection limits of morphine,O6‑Monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin were 100,50,50,100,20,20,10,1,and 0.01 ng/mL,respectively.The practical cases contained methamphetamine,codeine,cocaine,and O6‑monoacetylmorphine were detected accurately and rapidly.The method has the advantages of high sensitivity and good accuracy.The sample processing is simple and can be analyzed in a short time.This method is suitable for the analysis of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in some practical cases.展开更多
采用实时直接分析(direct analysis in real time,DART)离子化技术与四极杆-静电场轨道阱高分辨质谱联用技术(Q-Orbitrap MS),建立了快速定性和定量分析方法,检测豆制品中非法添加的碱性橙Ⅱ和金胺O。在正离子模式下,采用Full MS/target...采用实时直接分析(direct analysis in real time,DART)离子化技术与四极杆-静电场轨道阱高分辨质谱联用技术(Q-Orbitrap MS),建立了快速定性和定量分析方法,检测豆制品中非法添加的碱性橙Ⅱ和金胺O。在正离子模式下,采用Full MS/targetrd-MS2扫描方式,可直接将豆制品置于离子化区域内对两种染料进行快速定性分析。在定量分析中,采用80%乙腈水溶液提取样品,应用Dip-it载样方式,对实验参数进行系统性优化。采用空白基质溶液进行梯度稀释,测得碱性橙Ⅱ和金胺O在基质中的检出限均为0.2mg/L;采用内标法定量,在1～20mg/L浓度范围内线性关系良好,相关系数分别为0.997 2和0.999 4。该方法快速准确,可为食品中非法添加染料的定性和定量检测提供参考依据。展开更多
目的将实时直接分析离子源(direct analysis in real time,DART)与三重四极杆质谱联用,建立食品中非法添加PDE-5型抑制剂的快速筛查方法。方法3种不同基质样品加入乙腈振摇10 s后,设进样速率0.2 mm/s,进样体积4μl,选择DART离子源,离子...目的将实时直接分析离子源(direct analysis in real time,DART)与三重四极杆质谱联用,建立食品中非法添加PDE-5型抑制剂的快速筛查方法。方法3种不同基质样品加入乙腈振摇10 s后,设进样速率0.2 mm/s,进样体积4μl,选择DART离子源,离子化温度450℃,在正离子、多反应监测(MRM)下进行检测。结果该方法7种物质的检出限为0.025~12.5μg/g,检出阳性样品4种,分别非法添加羟基卡巴地那非、环己基去甲他达拉非、N-苄基他达拉非和N-苯基丙氧苯基卡巴地那非。结论该方法样品前处理简单,分析速度快,定性准确,环境污染小,能满足食品中非法添加PDE-5型抑制剂的快速筛查要求。展开更多
This paper,through mass spectrometric(MS)analysis for nitro compound explosives on a direct analysis in a real‑time time‑of‑flight MS,indicates that even on a high‑resolution MS with accurate mass measurement capabili...This paper,through mass spectrometric(MS)analysis for nitro compound explosives on a direct analysis in a real‑time time‑of‑flight MS,indicates that even on a high‑resolution MS with accurate mass measurement capabilities,there is no guarantee to obtain the unique molecular formula of a compound.By calculating spectra accuracy,highly accurate isotope pattern matching can be conducted to significantly improve performance of compound confirmation or identification.展开更多
Direct analysis in real time(DART) possesses the merits of analyzing sample in its native status with minimal or even no sample pretreatment.In this review, we summarized the recent applications of DART in the field o...Direct analysis in real time(DART) possesses the merits of analyzing sample in its native status with minimal or even no sample pretreatment.In this review, we summarized the recent applications of DART in the field of herbal medicine analysis such as compound detection, species identification, metabolites profiling and initial quantification. DART with the characters of hyper-rapid, easy-hyphenated offers a new research tool for herbal medicines to complete the experimental process in a very simple but still reliable way. It is anticipated that more wide and deep applications of DART in herbal medicine analysis, as rapid quantification, high-throughput active compounds screening, rapid species identification, and fast illegal additives screening will be promising and foreseeable in the near future.展开更多
基金financially supported by the Beijing Municipal Science and Technology Project, China (Z131110000613066)the Educational and Teaching Reform Project for Graduate Students, China (G-JG-XJ201408)the Beijing Key Laboratory of Bioprocess, China
文摘Access to security and safe food is a basic human necessity and essential for a sustainable world. To perform hi-end rood safety analysis and risk assessment with state of the art technologies is of utmost importance thereof. With applications as exemplified by microfiuidic immunoassay, aptasensor, direct analysis in real time, high resolution mass spectrometry, benchmark dose and chemical specific adjustment factor, this review presents frontier food safety analysis and risk assessment technologies, from which both food quality and public health will benefit undoubtedly in a foreseeable future.
基金support from National Natural Science Foundation of China(Nos.21172250 and 21275155).
文摘A method for rapid identification and quantification of phthalate plasticizers in beverages was developed.A number of 15 phthalate plasticizers which covered all the phthalates concerned in the US Consumer Product Safety Improvement Act(CPSIA),European Union legislations and Chinese national standards(GB)were analyzed.By a combined solid-phase micro-extraction(SPME)and direct analysis in real time mass spectrometry(DART-MS)approach,phthalates at sub-ng•mL^(−1)levels can be qualitatively and quantitatively analyzed in a short time.The use of ultrahigh-resolving power and the accurate mass measurement capacity naturally provided by Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS)minimizes the matrix interferences and thus enables the evaluation of phthalates in a complex matrix without extensive sample handlings or preparations.The limits of quantification(LOQs)were estimated to be at 0.3-5.0 ng•mL^(−1),lower than the Maximum Residue Limit(MRL)regulated by the European Union legislations(2007/19/EC)in foods,beverages,food packaging and toys(0.3-30 ng•mL^(−1)).This rapid and easy-to-use SPME-DART-FT-ICR-MS method provided a relatively high-throughput and powerful analytical approach for quick testing and screening phthalates in beverages and water samples to ensure food safety.
基金This research was supported by the Project for Strengthening the Police Force with Science and Technology(project number 2018GABJC29).
文摘We aimed to establish for the rapid detection of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in human blood and urine by direct analysis in real‑time coupled with tandem mass spectrometry(DART‑MS/MS).These samples were extracted by acetonitrile‑methanol(V/V=4:1),using DART 12 Dip‑it automatic sampling system.They were injected at 400℃,and analyzed by positive ion and multiple reaction monitoring mode.The detection limits of morphine,O6‑Monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin were 100,50,50,100,20,20,10,1,and 0.01 ng/mL,respectively.The practical cases contained methamphetamine,codeine,cocaine,and O6‑monoacetylmorphine were detected accurately and rapidly.The method has the advantages of high sensitivity and good accuracy.The sample processing is simple and can be analyzed in a short time.This method is suitable for the analysis of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in some practical cases.
文摘采用实时直接分析(direct analysis in real time,DART)离子化技术与四极杆-静电场轨道阱高分辨质谱联用技术(Q-Orbitrap MS),建立了快速定性和定量分析方法,检测豆制品中非法添加的碱性橙Ⅱ和金胺O。在正离子模式下,采用Full MS/targetrd-MS2扫描方式,可直接将豆制品置于离子化区域内对两种染料进行快速定性分析。在定量分析中,采用80%乙腈水溶液提取样品,应用Dip-it载样方式,对实验参数进行系统性优化。采用空白基质溶液进行梯度稀释,测得碱性橙Ⅱ和金胺O在基质中的检出限均为0.2mg/L;采用内标法定量,在1～20mg/L浓度范围内线性关系良好,相关系数分别为0.997 2和0.999 4。该方法快速准确,可为食品中非法添加染料的定性和定量检测提供参考依据。
文摘目的将实时直接分析离子源(direct analysis in real time,DART)与三重四极杆质谱联用,建立食品中非法添加PDE-5型抑制剂的快速筛查方法。方法3种不同基质样品加入乙腈振摇10 s后,设进样速率0.2 mm/s,进样体积4μl,选择DART离子源,离子化温度450℃,在正离子、多反应监测(MRM)下进行检测。结果该方法7种物质的检出限为0.025~12.5μg/g,检出阳性样品4种,分别非法添加羟基卡巴地那非、环己基去甲他达拉非、N-苄基他达拉非和N-苯基丙氧苯基卡巴地那非。结论该方法样品前处理简单,分析速度快,定性准确,环境污染小,能满足食品中非法添加PDE-5型抑制剂的快速筛查要求。
文摘This paper,through mass spectrometric(MS)analysis for nitro compound explosives on a direct analysis in a real‑time time‑of‑flight MS,indicates that even on a high‑resolution MS with accurate mass measurement capabilities,there is no guarantee to obtain the unique molecular formula of a compound.By calculating spectra accuracy,highly accurate isotope pattern matching can be conducted to significantly improve performance of compound confirmation or identification.
文摘Direct analysis in real time(DART) possesses the merits of analyzing sample in its native status with minimal or even no sample pretreatment.In this review, we summarized the recent applications of DART in the field of herbal medicine analysis such as compound detection, species identification, metabolites profiling and initial quantification. DART with the characters of hyper-rapid, easy-hyphenated offers a new research tool for herbal medicines to complete the experimental process in a very simple but still reliable way. It is anticipated that more wide and deep applications of DART in herbal medicine analysis, as rapid quantification, high-throughput active compounds screening, rapid species identification, and fast illegal additives screening will be promising and foreseeable in the near future.
