The Effect of the Direct Anti-Human Globulin Test on the Clinical Outcome of Patients Receiving Blood Transfusion

Objective:To study the effect of the direct anti-human globulin test on the clinical efficacy of blood transfusion patients.Methods:52 transfused patients were selected for this study,of which 26 cases with positive direct anti-human globulin tests were included in the positive group,and another 26 cases with negative direct anti-human globulin tests were included in the negative group.The apparent efficacy of the patients in the two groups after blood transfusion was compared.Results:After blood transfusion,the apparent efficacy of the negative group was significantly higher,P<0.05;in the positive group,the proportion of the predominantly multi-antibody group was the highest;after blood transfusion,the post-transfusion apparent efficacy of the simple IgG group was higher than that of the multi-antibody group,P<0.05;comparing the intensity of the different antibodies resulted in the 1+group,and the 3+to 4+groups were significantly lower after blood transfusion,P<0.05.Conclusion:The use of the direct antiglobulin test in transfused patients showed that patients with positive results would have better clinical efficacy.Direct anti-human globulin tests will have an impact on the clinical efficacy of blood transfusion in patients with positive results,so it is very important to carry out a direct anti-human globulin test on blood transfusion patients.

A novel method for geometric quality assurance of rock joint replicas in direct shear testing-Part 2:Validation and mechanical replicability

Each rock joint is unique by nature which means that utilization of replicas in direct shear tests is required in experimental parameter studies.However,a method to acquire knowledge about the ability of the replicas to imitate the shear mechanical behavior of the rock joint and their dispersion in direct shear testing is lacking.In this study,a novel method is presented for geometric quality assurance of replicas.The aim is to facilitate generation of high-quality direct shear testing data as a prerequisite for reliable subsequent analyses of the results.In Part 1 of this study,two quality assurance parameters,smf and V_(Hp100),are derived and their usefulness for evaluation of geometric deviations,i.e.geometric reproducibility,is shown.In Part 2,the parameters are validated by showing a correlation between the parameters and the shear mechanical behavior,which qualifies the parameters for usage in the quality assurance method.Unique results from direct shear tests presenting comparisons between replicas and the rock joint show that replicas fulfilling proposed threshold values of σ_(mf)<0.06 mm and
|V_(Hp100)|
<0.2 mm have a narrow dispersion and imitate the shear mechanical behavior of the rock joint in all aspects apart from having a slightly lower peak shear strength.The wear in these replicas,which have similar morphology as the rock joint,is in the same areas as in the rock joint.The wear is slightly larger in the rock joint and therefore the discrepancy in peak shear strength derives from differences in material properties,possibly from differences in toughness.It is shown by application of the suggested method that the quality assured replicas manufactured following the process employed in this study phenomenologically capture the shear strength characteristics,which makes them useful in parameter studies.

Tensile strength and failure behavior of rock-mortar interfaces: Direct and indirect measurements

The tensile strength at the rock-concrete interface is one of the crucial factors controlling the failure mechanisms of structures,such as concrete gravity dams.Despite the critical importance of the failure mechanism and tensile strength of rock-concrete interfaces,understanding of these factors remains very limited.This study investigated the tensile strength and fracturing processes at rock-mortar interfaces subjected to direct and indirect tensile loadings.Digital image correlation(DIC)and acoustic emission(AE)techniques were used to monitor the failure mechanisms of specimens subjected to direct tension and indirect loading(Brazilian tests).The results indicated that the direct tensile strength of the rock-mortar specimens was lower than their indirect tensile strength,with a direct/indirect tensile strength ratio of 65%.DIC strain field data and moment tensor inversions(MTI)of AE events indicated that a significant number of shear microcracks occurred in the specimens subjected to the Brazilian test.The presence of these shear microcracks,which require more energy to break,resulted in a higher tensile strength during the Brazilian tests.In contrast,microcracks were predominantly tensile in specimens subjected to direct tension,leading to a lower tensile strength.Spatiotemporal monitoring of the cracking processes in the rock-mortar interfaces revealed that they show AE precursors before failure under the Brazilian test,whereas they show a minimal number of AE events before failure under direct tension.Due to different microcracking mechanisms,specimens tested under Brazilian tests showed lower roughness with flatter fracture surfaces than those tested under direct tension with jagged and rough fracture surfaces.The results of this study shed light on better understanding the micromechanics of damage in the rock-concrete interfaces for a safer design of engineering structures.

Extended research on software hybrid testing combining reliability and directed testing

The software reliability testing has many disadvantages in practice, such as high complexity of constructing operational profiles and poor fault detection efficiency. Oppositely, the directed testing with a high fault detection rate is incapable of estimating reliability quantificationally. To solve this problem, a hybrid testing combining reliability and directed testing as well as a reliability model based on the order statistic （OS） model were presented by Mitchell. An extended research on Mitchell＇s work is proposed. Firstly, the most proper distribution of the fault＇s failure rate which tends to be Iognormal is suggested, and a detailed form of the OS model based on Iognormal and the corresponding parameter estimation method are proposed, respectively. Secondly, an im- plementing framework for the hybrid testing is proposed. Finally, the hybrid testing and the OS model are applied on a real website system. The experimental results indicate： the hybrid testing has more efficient fault detection power and lower testing cost than the reliability testing; compared with three traditional software reliabil ity growth models, the OS model has a best or pretty estimation or prediction power for each data set; and for the failure data set collected from hybrid testing, the OS model also achieves an ac- ceptable estimation result.

Effects of water saturation and loading rate on direct shear tests of andesite

For estimating the long-term stability of underground framework,it is vital to learn the mechanical and rheological characteristics of rock in multiple water saturation conditions.However,the majority of previous studies explored the rheological properties of rock in air-dried and water saturated conditions,as well as the water effects on compressive and tensile strengths.In this study,andesite was subjected to direct shear tests under five water saturation conditions,which were controlled by varying the wetting and drying time.The tests were conducted at alternating displacement rates under three vertical stresses.The results reveal that the shear strength decreases exponentially as water saturation increases,and that the increase in shear strength with a tenfold increase in displacement rate is nearly constant for each of the vertical stresses.Based on the findings of the shear tests in this study and the compression and tension tests in previous studies,the influences of both water saturation and loading rate on the Hoek-Brown failure criterion for the andesite was examined.These results indicate that the brittleness index of the andesite,which is defined as the ratio of uniaxial compressive strength to tensile strength,is independent of both water saturation and loading rate and that the influences of the water saturation dependence and the loading rate dependence of the failure criterion can be converted between each other.

Mesomechanical simulation of direct shear test on outwash deposits with granular discrete element method

The mechanical properties of outwash deposits which are taken as unconsolidated geo-materials with the characteristics of non-uniformity, heterogeneity and multiphase have attracted much attention in engineering. According to the results of laboratory direct shear test on the remolded samples, the soil particle parameters of numerical model based on in-situ particle size cumulative curves and 3D granular discrete element method were determined. Then, numerical experiments on different lithology, stone content and gradation composition were conducted. The results show that it is not a flat surface but a shear band that yields in the sample. The curve of particle velocity vs distance from the designed shear surface of test model that is taken as a datum plane in the vertical section of sample shows in "S" shape. The shear disturbance area is about twice the maximum diameter of stone blocks. The greater the stiffness of stone is, the rougher the shear surface is. The shear strength of outwash deposits is largely controlled by lithology and stone content, and the bite force between stone blocks is the root reason of larger friction angle. It is also shown that strain hardening and low shear dilatancy occur under high confining pressure as well as possibility of shear shrinkage. But it is easy to behave shear dilatation and strain softening under low confining pressure. The relationship between particle frictional coefficient and stone content presents an approximately quadratic parabola increase. The strain energy first increases and then drops with the increase of frictional energy. The cohesion increases with soil stiffness increasing but decreases with stone stiffness increasing. Numerical results are consistent with the laboratory test results of remolded samples, which indicate that this method can be a beneficial supplement to determine the parameters of engineering deposit bodies.

ANALYSIS OF SIZE EFFECT,SHEAR DEFORMATION AND DILATION IN DIRECT SHEAR TEST BASED ON GRADIENT- DEPENDENT PLASTICITY

The paper concerns the issue of size law,localized deformation and dilation or compaction due to shear localization. It is assumed that the shear localization initiates at the peak shear stress in the form of single shear band,and based on gradient-dependent plasticity,an analytical solution on size effect or snap-back is obtained. The results show that the post peak response becomes steeper and even exhibits snap-back with increasing of length. For small specimen,the relative shear displacement when specimen failure occurs is lower than that of larger specimen and the shear stress-relative displacement curve becomes steeper. The theoretical solution on non-uniformity of strains in shear band is obtained and evolution of the relative shear displacement is represented. By resorting to the linear relation between local plastic shear strain and local plastic volumetric strain,the dilation and compaction within shear band are analyzed. Relation between apparent shear strain and apparent normal strain and relation between shear displacement and vertical displacement are established.

Large-scale direct shear testing of geocell reinforced soil

The tests on the shear property of geocell reinforced soils were carried out by using large-scale direct shear equipment with shear-box-dimensions of 500 mm×500 mm×400 mm (length×width×height). Three types of specimens, silty gravel soil, geocell reinforced silty gravel soil and geocell reinforced cement stabilizing silty gravel soil were used to investigate the shear stress-displacement behavior, the shear strength and the strengthening mechanism of geocell reinforced soils. The comparisons of large-scale shear test with triaxial compression test for the same type of soil were conducted to evaluate the influences of testing method on the shear strength as well. The test results show that the unreinforced soil and geocell reinforced soil give similar nonlinear features on the behavior of shear stress and displacement. The geocell reinforced cement stabilizing soil has a quasi-elastic characteristic in the case of normal stress coming up to 1.0 GPa. The tests with the reinforcement of geocell result in an increase of 244% in cohesion, and the tests with the geocell and the cement stabilization result in an increase of 10 times in cohesion compared with the unreinforced soil. The friction angle does not change markedly. The geocell reinforcement develops a large amount of cohesion on the shear strength of soils.

Numerical simulation of direct shear tests on mechanical properties of talus deposits based on self-adaptive PCNN digital image processing

The macro mechanical properties of materials with characteristics of large scale and complicated structural composition can be analyzed through its reconstructed meso-structures.In this work,the meso-structures of talus deposits that widely exist in the hydro-power engineering in the southwest of China were first reconstructed by small particles according to the in-situ photographs based on the self-adaptive PCNN digital image processing,and then numerical direct shear tests were carried out for studying the mechanical properties of talus deposits.Results indicate that the reconstructed meso-structures of talus deposits are more consistent with the actual situation because the self-adaptive PCNN digital image processing has a higher discrimination in the details of soil-rock segmentation.The existence and random distribution of rock blocks make the initial shear stiffness,the peak strength and the residual strength higher than those of the "pure soil" with particle size less than 1.25 cm apparently,but reduce the displacements required for the talus deposits reaching its peak shear strength.The increase of rock proportion causes a significant improvement in the internal friction angle of talus deposit,which to a certain degree leads to the characteristics of shear stress-displacement curves having a changing trend from the plastic strain softening deformation to the nonlinear strain hardening deformation,while an unconspicuous increase in cohesion.The uncertainty and heterogeneity of rock distributions cause the differences of rock proportion within shear zone,leading to a relatively strong fluctuation in peak strengths during the shear process,while movement features of rock blocks,such as translation,rotation and crossing,expand the scope of shear zone,increase the required shear force,and also directly lead to the misjudgment that the lower shear strength is obtained from the samples with high rock proportion.That,however,just explains the reason why the shear strength gained from a small amount of indoor test data is not consistent with engineering practice.

Evaluation of a Direct Rapid Immunohistochemical Test (dRIT) for Rapid Diagnosis of Rabies in Animals and Humans

Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay ( dFA) which is very expensive and requires a high level of expertise. There is a need for more economical and user friendly tests, particularly for use in developing countries. We have established one such test called the direct rapid immunohistochemical test (dRIT) for diagnosis of rabies using brain tissue. The test is based on capture of rabies nucleoprotein (N) antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counter staining with haematoxollin. The test was done in parallel with standard FAT dFA using 400 brain samples from different animals and humans. The rabies virus N protein appears under light microscope as reddish brown particles against a light blue background. There was 100 % correlation between the results obtained by the two tests. Also, interpretation of results by dRIT was easier and only required a light microscope. To conclude, this newly developed dRIT technique promises to be a simple, cost effective diagnostic tool for rabies and will have applicability in field conditions prevalent in developing countries.

Application of bi-directional static loading test to deep foundations

Bi-directional static loading test adopting load cells is widely used around the world at present, with increase in diameter and length of deep foundations. In this paper, a new simple conversion method to predict the equivalent pile head load-settlement curve considering elastic shortening of deep foundation was put forward according to the load transfer mechanism. The proposed conversion method was applied to root caisson foundation in a bridge and to large diameter pipe piles in a sea wind power plant. Some new load cells, test procedure, and construction technology were adopted based on the applications to different deep foundations, which could enlarge the application scopes of bi-directional loading test. A new type of bi-directional loading test for pipe pile was conducted, in which the load cell was installed and loaded after the pipe pile with special connector has been set up. Unlike the conventional bi-directional loading test, the load cell can be reused and shows an evident economic benefit.

Design and verification of portable direct shear tester with application to remolded colluvium geomaterials

The mechanical properties of colluvium strongly govern the stability of colluvial slopes, and they arc essential for the related analysis and design. Nevertheless, their measurement is not easy on account of heterogeneity in property and difficulty of sampling. This study attempted to evaluate the shear strength of remolded colluvium by means of a simple direct shear test in the field. A portable direct shear tester was designed to overcome the inconvenience and expensiveness of the conventional large-scale in-situ direct shear test. It can be easily assembled and applied for the silnplc field direct shear test. For calibration, the results of the portable direct shear tester were compared with the results of the laboratory direct shear tester for four different types of soil samples, i.e. standard sand, slate debris, arenaceous shale debris and mixture of gravel and sand. Correlation formulas were established based on the calibration, enabling the portable direct shear tester to measure and estimate the shear strength of remoldcd colluvium in field. This study primarily focuses on the colluvium in Central Taiwan, including the lateritic Dadu Terrace and the arcnaceous shale of Taiping-Wufcng mounts. The portable direct shear tester was applied to sites selected in these areas, and the results were furthcr analyzed and discussed.

Direct antiglobulin test-negative autoimmune hemolytic anemia in a patient withβ-thalassemia minor during pregnancy:A case report

BACKGROUND Severe refractory anemia during pregnancy can cause serious maternal and fetal complications.If the cause cannot be identified in time and accurately,blind symptomatic support treatment may cause serious economic burden.Thalassemia minor pregnancy is commonly considered uneventful,and the condition of anemia rarely progresses during pregnancy.Autoimmune hemolytic anemia(AIHA)is rare during pregnancy with no exact incidence available.CASE SUMMARY We report the case of a 30-year-oldβ-thalassemia minor multiparous patient experiencing severe refractory anemia throughout pregnancy.We monitored the patient closely,carried out a full differential diagnosis,made a diagnosis of direct antiglobulin test-negative AIHA,and treated her with prednisone and intravenous immunoglobulin.The patient gave birth to a healthy full-term baby.CONCLUSION Coombs-negative AIHA should be suspected in cases of severe hemolytic anemia in pregnant patients with and without other hematological diseases.

Direct shear tests of coarse-grained fillings from high-speed railway subgrade in cold regions

In order to study the shear behavior of coarse-grained fillings taken from the subgrade bottom layer of a cold region high-speed railway,large scale direct shear tests were conducted with different normal pressures,water contents and temperatures.The results indicate that the relationship between shear displacement and shear stress changed from strain-softening at lower normal pressures to strain-hardening at higher normal pressures,in both unfrozen and frozen states.This phenomenon was mainly due to the shear dilatation deformation effect.The shear displacement-shear stress curves show similar stages.Besides,the shear stress rapidly increased and there was not an increment in the shear displacement during the initial stage of the shear process in the frozen state.In both the unfrozen or frozen states at the same water contents,the shear strength increased with increasing normal pressure.

Mathematical Modeling of Shear Stress and Direct Shear Test for Compressible Soil: Case of Soil Bordering the Wouri River

This paper focuses on the development of the mathematical model of shear stress by direct shear test for compressible soil of the littoral region, which will be a great tool in the hand of geotechnical engineers. The most common use of a shear test is to determine the shear strength which is the maximum shear stress that a material can withstand before the failure occurs. This parameter is useful in many engineering designs such as foundations, roads and retaining walls. We carried out an experimental laboratory test of ten samples of undisturbed soil taken at different points of the border of Wouri river of Cameroon. The samples were collected at different depths and a direct shear test was conducted. The investigations have been performed under constant vertical stresses and constant sample volume with the aim to determine the frictional angle and the cohesion of the compressible soil which are so important to establish the conditions of buildings stability. Special care was taken to derive loading conditions actually existing in the ground and to duplicate them in the laboratory. Given that the buildings constructed in this area are subjected to settlement, landslide, and punch break or shear failure, the cohesion and the frictional angle are determined through the rupture line after assessed the mean values of the shear stress for the considered ten samples. The bearing capacity of the soil, which is the fundamental soil parameter, was calculated. From the laboratory experimental results, the least squared method was used to derive an approximated mathematical model of the shearing stress. Many optimizations methods were then considered to reach the best adjustment.

Presentation of Empirical Equations for Estimating Internal Friction Angle of GW and GC Soils in Mashhad, Iran Using Standard Penetration and Direct Shear Tests and Comparison with Previous Equations

Presentation of empirical equations for estimating engineering properties of soils is a simple, low cost and widely-used method. One of the major concerns in using these equations is evaluating their accuracy in different conditions and regions which often lead to doubts about obtained results. Most of these equations were derived in special laboratories, different climate conditions and in soils with different geotechnical and geological engineering properties and were generalized to other conditions. The main question is that whether these methods are also applicable to other conditions. Using local equations and narrowing the usage range of various methods based on each region’s properties are appropriate methods to solve these problems. This leads to simplified and faster analysis and high reliability in the obtained results. In this paper, empirical equations were derived to estimate internal friction angle, based on SPT numbers of Mashhad City’s soils in Iran, using SPT and direct shear tests results from 50 samples (25 GW and 25 GC soil samples). The results showed similar values for predicted?φ?values by SPT test and?φ?values determined by direct shear tests.

Three-Dimensional Model Test for Port Engineering with Multi-Directional Waves

-The necessity of using irregular waves, especially multi- directional waves to conduct three-dimensional model tests for port engineering and the test method are described in this paper through an example of model test for a port. The test results show that a deep navigation channel has a large effect on the waves in front of the breakwater near the port entrance and on the wave condition in the port.

Research on Directional Responsetest of Pyranometer

Directional response is one of the most important parameters of pyranometer,which is crucial to evaluate the performance of the pyranometer by determining the responsivityand the corresponding radiation for solar beam radiation from different directions of the pyranometer. In the paper,the method for testing directional error is present,four pyranometers are tested,after data processing,the directional errors of a secondary standard CM P22 and a first class CM P11 are 4.5 W/m^2 and 8.3 W/m^2 respectively,which are within the nominal specification given by the manufacturer. M eanwhile,the directional error of a first class pyranometerF S-S6 is 11.0 W/m^2,while the value of a second class pyranometer TBQ-2-B is15.2 W/m^2,which both meet the specification for classification. The results showthat these four pyranometers can reach the levels recommended by WM O and ISO,which validates that the method is feasible and the China made pyranometers are reliable.

砂土-混凝土接触面剪切带细观变形特征的试验研究

接触面剪切带的细观变形特征是研究结构物与土体相互作用的核心问题。为探明砂土-混凝土接触面剪切带变形特征,利用大型直剪仪开展砂土-混凝土接触面直剪试验,采用灌砂变形标记法实测砂土变形,依据标记线剪应变沿竖向的变化特征对砂土变形区域进行划分。在此基础上,研究粗糙度、法向应力和密实度对砂土-混凝土接触面剪切带细观变形特征的影响规律。研究结果表明:1)砂土可分为非剪切区、过渡区和应变集中区,应变集中区仅存在剪切面之上。2)规则型接触面的上剪切带厚度均值为33D_(50),远大于下剪切带9D_(50)和“光滑”接触面剪切带厚度20.4D_(50)。3)密实度对剪切带及其过渡区厚度的影响微弱,而粗糙度对其影响显著;法向应力、密实度对应变集中区厚度影响有限,应变集中区厚度随粗糙度的增大近似线性增大;应变集中区长度随粗糙度的增大先增大后减小,随密实度的增大近似线性增大,随法向应力增大而减小。4)随剪切位移增大,上剪切带厚度不断增大而下剪切带厚度先增大后保持基本不变,应变集中区的长度增大,但剪切位移对应变集中区厚度的影响有限。5)获得归一化剪切带厚度取值h_(t)/D_(50)与D_(50)的关系,在有限元模拟规则型结构与砂土的相互作用时,建议砂土体细化网格单元的范围不小于最大剪切带厚度37D_(50)。研究成果深化了砂土-规则型接触面剪切带变形特征的认识,可为大粗糙度、带肋桩的承载变形特性的精细化有限元模拟分析提供重要参考和依据。