[Objectives] This study was conducted to explore the effects of different N application rates and densities on the growth and development of direct-seeding rape as well as on its yield.[Methods] A field experiment was...[Objectives] This study was conducted to explore the effects of different N application rates and densities on the growth and development of direct-seeding rape as well as on its yield.[Methods] A field experiment was carried out by setting five N fertilizer treatments and three density levels.[Results] Increasing seeding density and N application rate could improve the seed yield of rape. The suitable N application rate for the three densities were calculated using the fitted fertilizer efficiency models, respectively, to be 186.77, 221.35 and 236.14 kg/hm^2, at which the yields were the highest. The results showed that in this area, the seeding density of direct-seeding rape could be selected in the range of 6.0×10^5-7.5×10^5 plants/hm^2, and the suitable N application rate could be in the range of 221.35-236.14 kg/hm^2.[Conclusions] This study provides a theoretical basis for the maximization of spatial resource and efficient utilization of fertilizer.展开更多
[Objective] This study aimed to investigate the effect of direct-seeding with non-flooding and wheat residue returning patterns on greenhouse gas emission from rice paddy. [Method] Two rice cultivars currently used in...[Objective] This study aimed to investigate the effect of direct-seeding with non-flooding and wheat residue returning patterns on greenhouse gas emission from rice paddy. [Method] Two rice cultivars currently used in the production, Yangdao 6 (an indica) and Yangjing 4038 (a japonica), were field grown using a direct-seeding method, and four treatments, wheat straw incorporation into soil and traditional flooding (SlF), non-flooding and wheat straw mulching (NSM), non-flooding and wheat straw incorporation into soil (NSl) and traditional flooding (no straw returned, Control, TF), were imposed after sowing to maturity. Effects of direct-seeding with non-flooding and wheat residue returning patterns on CH4, N20 and CO2 emissions were investigated by using the method of static chamber-gas chromatographic tech- niques. [Result] Grain yield showed no significant difference between non-flooding and flooding treatments, but was significantly higher under the SlF than under any other treatments. The emission flux of CH4 and CO2 under TF and SlF exhibited a single peak curve, while changed little under the NSl and NSM The emission flux of N2Oshowed multiple perk curves for all the treatments. Compared with TF, SlF significantly increased mean emission flux of CH4 or N2O, decreased emission of N20, while NSl and NSM significantly decreased the mean emission flux of OH4, and increased emission flux of N2O and CO2. SIF also increased Green Warm Potential (GWP) of CH4, N2O and CO2 and the GWP per unit grain yield by 47.3%- 53.7% and 32.2%-39.4%, respectively. Both NSl and NSM decreased GWP by 24.2%-29.6% and 30.1%-35.5%, and the GWP per unit grain yield was decreased by 21.7-27.2% and 25.6%-31.1%, respectively. [Conelusion] both NSl and NSM could significantly reduce greenhouse effect of CH4, N2O and CO2 meanwhile maintain a high grain yield.展开更多
Plant-mediated RNA interference(RNAi)has emerged as a promising technology for insect control.The green peach aphid,Myzus persicae,feeds on over 400 species of host plants.Brassica napus(rape)is the second most import...Plant-mediated RNA interference(RNAi)has emerged as a promising technology for insect control.The green peach aphid,Myzus persicae,feeds on over 400 species of host plants.Brassica napus(rape)is the second most important oilseed crop worldwide.Myzus persicae is highly reproductive and causes severe damage to the rape plants due to its quite flexible life cycle.In this study,we tested the RNAi effects of transgenic rape plants on M.persicae.By in vitro feeding M.persicae with artificial diets containing double-stranded RNAs(dsRNAs)targeting seven aphid genes,we identified a new gene encoding the partitioning-defective protein 6(Par6)as the most potent RNAi target.Tissue-and stage-expression analysis of Par6 suggested this gene is highly expressed in the embryo and adult stage of M.persicae.We next generated transgenic rape plants expressing ds Par6 by Agrobacteriummediated transformation and obtained nine independent transgenic lines.Compared to wild-type control plants,transgenic rape lines expressing ds Par6 showed strong resistance to M.persicae.Feeding assays revealed that feeding transgenic rape plants to M.persicae significantly decreased MpPar6 expression and survival rate and impaired fecundity.Furthermore,we showed that the resistance levels to M.persicae are positively correlated with ds Par6 expression levels in transgenic rape plants.Our study demonstrates that transgenic rape plants expressing ds Par6 are efficiently protected from M.persicae.Interfering with the genes involved in embryo development could be the effective RNAi targets for controlling aphids and potentially other insect pests.展开更多
The sustainability of rice production continues to be a subject of uncertainty and inquiry attributed to shifts in climatic conditions. In light of the impending climate change crisis and the high labor and water cost...The sustainability of rice production continues to be a subject of uncertainty and inquiry attributed to shifts in climatic conditions. In light of the impending climate change crisis and the high labor and water costs accompanying it, direct-seeded rice(DSR) is unquestionably one of the most practical solutions. Despite its resource and climate-friendly advantages, early maturing rice faces weed competitiveness and seedling establishment challenges. Resolving these issues is crucial for promoting its wider adoption among farmers, presenting it as a more effective sustainable rice cultivation method globally. Diverse traditional and contemporary breeding methods are employed to mitigate the limitations of the DSR approach, leveraging advanced techniques such as speed breeding and genome editing. Focusing on key traits like mesocotyl length elongation, early seedling vigor, root system architecture, and weed competitiveness holds promise for transformative improvements in DSR adaptation at a broader scale within farming communities. This review aims to summarize how these features contribute to increased crop production in DSR conditions and explore the research efforts focusing on enhancing DSR adaptation through these traits. Emphasizing the pivotal role of these game-changing traits in DSR adaptation, our analysis sheds light on their potential transformative impact and offers valuable insights for advancing DSR practices.展开更多
The cold regions are located in high latitudes and cold climates.The local excellent ornamental plant resources are relatively scarce.The plant species that are suitable for both ornamental and productive benefits of ...The cold regions are located in high latitudes and cold climates.The local excellent ornamental plant resources are relatively scarce.The plant species that are suitable for both ornamental and productive benefits of landscape of flower sea construction are also even fewer.Therefore,it is imperative to introduce and screen the plant resources suitable for cold regions to create the landscape of flower sea.The rape,an oilseed crop,was used as a research object in order to create a productive flower landscape with both ornamental and economic values in cold regions.Four rape flower varieties,Qingza No.5,7,9,and 11,were introduced from Qinghai Hufeng Agricultural Science and Technology Group Co.,Ltd.They were planted in the experimental practice base of Northeast Agricultural University in three batches.Development characteristics and seed yield of rape flowers on different sowing dates were studied.The fuzzy probability method was used to comprehensively evaluate the varieties.The results showed that the rape flowers grew well in Harbin City during the experimental sowing period,which could form a good landscape of flower sea and had a considerable rapeseed yield.It could be widely used in cold urban and rural areas,such as Harbin City.In view of the experimental results,the strategies of creating a productive landscape of rape flower sea were proposed and the economic benefits were analyzed.It could change the status quo of a uniform landscape of flower sea in cold regions,help the development of rural tourism,and promote local economic income.展开更多
Field experiments were carried out to study the effect of different foliar fertilizer treatments on the agronomic traits,yield,and quality of Brassica napus L.for both vegetable and oil dual-use rape.The seedlings of...Field experiments were carried out to study the effect of different foliar fertilizer treatments on the agronomic traits,yield,and quality of Brassica napus L.for both vegetable and oil dual-use rape.The seedlings of‘Youtai 929’,‘Jinyoutai 1’,and‘Zhongshuang 11’were sprayed with potassium dihydrogen phosphate,naphthylacetic acid,and selenium fertilizer,and the agronomic traits,yield,and quality were determined.The results showed that the naphthylacetic acid treatment had the highest indexes before winter,followed by the selenium fertilizer treatment.At the harvesting stage,the plant height,number of siliquae,yield per plant,and number of effective branches on the main stem of the three varieties were the highest in the naphthylacetic acid treatment,followed by those in the potassium dihydrogen phosphate treatment.The fresh shoot quality was the best in the selenium fertilizer treatment and the second in the naphthylacetic acid treatment.‘Jinyoutai 1’applied with naphthylacetic acid had the highest economic benefit of 4971.78 CNY/667m^(2),which was 642.32 CNY/667m^(2) higher than that of the control.The findings suggested that naphthylacetic acid was suitable to be used as the foliar fertilizer for the seedlings of B.napus varieties for both vegetable and oilseed,and the additional application of selenium fertilizer demonstrated better effect.展开更多
Phosphorus(P)is essential for living plants,and P deficiency is one of the key factors limiting the yield in rapeseed production worldwide.As the most important organ for plants,root morphology traits(RMTs)play a key ...Phosphorus(P)is essential for living plants,and P deficiency is one of the key factors limiting the yield in rapeseed production worldwide.As the most important organ for plants,root morphology traits(RMTs)play a key role in P absorption.To investigate the genetic variability of RMT under low P availability,we dissected the genetic structure of RMTs by genome-wide association studies(GWAS),linkage mapping and candidate gene association studies(CGAS).A total of 52 suggestive loci were associated with RMTs under P stress conditions in 405 oilseed rape accessions.The purple acid phosphatase gene BnPAP17 was found to control the lateral root number(LRN)and root dry weight(RDW)under low P stress.The expression of BnPAP17 was increased in shoot tissue in P-efficient cultivars compared to root tissue and P-inefficient cultivars in response to low P stress.Moreover,the haplotype of BnPAP17^(Hap3)was detected for the selective breeding of P efficiency in oilseed rape.Over-expression of the BnPAP17^(Hap3)could promote the shoot and root growth with enhanced tolerance to low P stress and organic phosphorus(Po)utilization in oilseed rape.Collectively,these findings increase our understanding of the mechanisms underlying BnPAP17-mediated low P stress tolerance in oilseed rape.展开更多
[Objective] The aim of the research was to find the optimal nitrogen application rate, density and seedling age for no-tillage rape in seedling stage. [Method] With the D-optimal quadratic regression design for three ...[Objective] The aim of the research was to find the optimal nitrogen application rate, density and seedling age for no-tillage rape in seedling stage. [Method] With the D-optimal quadratic regression design for three factors, the 310 scheme was designed to study the effects of nitrogen application rate, density and seedling age on dry matter accumulation of no-tillage rape in seedling stage. [Result] With the increase of nitrogen application rate, density and seedling age, the dry matter content appeared like a parabola, increasing firstly and then declining. The change of nitrogen application rate caused greater influence than that of density and seedling age; the interaction effects between nitrogen application rate and density were greater than that between nitrogen application rate and seedling age as well as between density and seedling age. [Conclusion] Considered comprehensively, the dry matter content of no-tillage rape in seedling stage reached the highest level (4 768.2 kg/hm2) when the nitrogen application rate, the density and the seedling age were 195 kg/hm2, 93 000 plants/hm2 and 33 d, respectively.展开更多
[Objective] To study the correlation between the biomechanical properties of rape stalks and rape stem lodging. [Method] Through axial compression tests to the stalks of 4 different rape varieties, the change rules of...[Objective] To study the correlation between the biomechanical properties of rape stalks and rape stem lodging. [Method] Through axial compression tests to the stalks of 4 different rape varieties, the change rules of maximum stem bearing ca- pacity, maximum compressive strength, elastic modulus and moment of inertia along plant height were analyzed, as well as the effect of different varieties and water contents on the biomechanical property indices of rape stalks. [Result] The maximum loads of rape stalks presented liner decrease trend along with the increase of stem height, and all reached the maximums below the height of 50 cm. The maximum stem compressive strength and elastic modulus of the 4 varieties were increased with ascending height, but in a slow rate with small change, thus the modulus of e- lasticity could be considered as unchanged. The maximum bearing capacity, maxi- mum compressive strength and elastic modulus of dry rape stalks were higher than wet stalks, indicating that the water contents of rape stalks had significant effect on their mechanical properties. According to the actual lodging situations in filed, stalks of variety No. 1 owned the worst biomechanical properties and lodging degree, while the biomechanical properties of No. 6 and F5 were better than No. 1 and No. 9, and they also had stronger lodging-resistance. [Conclusion] The study provides parameters and bases for the design of mechanized production and mechanical deep processing of crops, and can better reveal the physical natures of organisms. The methods used in this study can also be used to screen excellent crop stalks.展开更多
[Objective] This study aimed to investigate the correlation between yield and quality traits of rape. [Method] Taking "Sanbei 98" and "Youyan 599" as ex- periment materials, correlation between yield and quality t...[Objective] This study aimed to investigate the correlation between yield and quality traits of rape. [Method] Taking "Sanbei 98" and "Youyan 599" as ex- periment materials, correlation between yield and quality traits of Brassica napus L. cultivars was explored by orthogonal rotation method. [Result] The results showed that: 1 In both the two varieties-Sanbei98 and Youyan599, erucic acid content had the largest coefficient of variation, but its average content was lower than 2% in both the two varieties (0.90% in Sanbei98 and 1.24% in Youyan599), and the palmitic acid content had the smallest coefficient of variation. 2 In terms of the dif- ferences in quality traits at different yield levels, only the mean of eicosenoic acid content revealed significant difference in Sanbei98, and only the mean of seed-pro- tein content revealed significant difference in Youyan599, and there was no signifi- cant difference in any combined quality traits in both the two varieties. 3 The yield was negatively correlated with the erucic acid content or oil content, and posi- tively correlated with the content of seed-protein, linoleic acid, or linolenic acid, and the coefficient of correlation between the yield and either of the contents of glucosi- nolates, oleic acid, palmitic acid, stearic acid, and eicosenoic acid was smaller. ~, when the yield of a variety increased considerably, there was a certain decreasing trend in erucic acid content, oil content, the total amount of erucic chain fatty acids (El & Er), while there was an increasing trend in seed-protein content, linoleic acid content, linolenic acid content, saturated fatty acids (SFA), the total amount of fat and protein (P & F), unsaturation index (IUFA), unsaturated fatty acids (UFA), the total amount of 18 carbon fatty acids (18-C FA), unsaturated fatty acids [UFA/(Ei & Er) ], and the sum of oleic acid and linoleic acid (OI & Li). 5 When the seed-pro- tein content is increased, the quality of rape oil cake is also improved; when the unsaturated index is increased, the oil stability is decreased. The increase in the total amount of unsaturated fatty acids, oleic acid and linoleic acid helps to increase in the total amount of fatty acids absorbed by human bodies. [Conclusion] This study provides a theoretical foundation for the cultivation of high-yielding and top- quality hybrid rape cultivars.展开更多
[Objective]The paper was to investigate the dynamics of essential metal elements(Ca,Mg,Fe,Mn,Zn and Cu)contents in winter oilseed rape(Brassica napus L.)during cold acclimation,and to reveal the effects and mechan...[Objective]The paper was to investigate the dynamics of essential metal elements(Ca,Mg,Fe,Mn,Zn and Cu)contents in winter oilseed rape(Brassica napus L.)during cold acclimation,and to reveal the effects and mechanisms of essential elements in cold resistance of oilseed rape.[Method]Three varieties with different cold resistance including Zhongshuang No.11(ZS,freezing sensitive variety),Ganyouza No.1(GY)and Jinyuyou No.1(JYY,freezing tolerant varieties)were used in the study,and the changes of necessary metal elements in these oilseed rapes during cold acclimation were studied.[Result]The concentration of metal elements in oilseed rape had significant changes during cold acclimation,and the difference between varieties was significant.In plant roots,the concentrations of Ca,Mg,Fe,Mn,Zn and Cu were significantly increased,this might because that low temperature made the transpiration decreased,thus blocking the upward transport of elements.In plant shoots,the concentrations of Ca,Mg and Zn were significantly decreased,while the concentrations of Fe and Mn were significantly increased;the change extent of various elements in ZS variety was the largest.[Conclusion]Maintaining the stability of Ca,Mg and Zn concentrations and increasing Fe,Mn and Cu concentrations in plant shoots might be benefit for increasing the cold tolerance of winter oilseed rape.展开更多
[ Objective] The aim of the research was to provide reference for reasonable application of nitrogen fertilizer for high yield.cultivation of hybrid rape cuhivar Youyan 9 and Youyan 10. [ Method] The net increment cha...[ Objective] The aim of the research was to provide reference for reasonable application of nitrogen fertilizer for high yield.cultivation of hybrid rape cuhivar Youyan 9 and Youyan 10. [ Method] The net increment changes of individual plant fresh weight and dry matter weight of Youyan 9 and Youyan 10 with different nitrogen application treatments were studied. [ Result] The differences among average fresh weight increments of individual plant and average dry matter weight increment of individual plant with different treatments reached 0. 01 extremely significant level. Fresh weight increment and dry matter weight net increment of individual plant declined gradually with the increase of nitrogen application. In growtheourse ,fresh weight net increment of individual plant increased firstly then decreased and the maximum was in beginning flowering stage, besides that dry matter net increment increased gradually and the maximum was in mature period. The correlations among fresh net increment, dry matter weight net increment and yield net increment were positive or extremely positive. [ Conclusion] Under experimental condition, when nitrogen application was 225 kg/hm^2, hybrid rape Yanyou 9 and Yanyou 10 with low erucic,low glucosinolate could obtain high yield.展开更多
[Objective] This study aimed to provide reliable theoretical basis for re- source utilization of sludge. [Method] By using pot experiment, variations of the yield index and salt content of rape and the accumulation of...[Objective] This study aimed to provide reliable theoretical basis for re- source utilization of sludge. [Method] By using pot experiment, variations of the yield index and salt content of rape and the accumulation of three kinds of heavy metals in rape plants and soil including Hg, As and Zn were explored. [Result] The results showed that medium sludge level (50 g/pot) significantly increased the rape yield and root/shoot ratio; when the application amount of sludge reached 100 g/pot, the fertil- ization effect of sludge on potted rape yield was significantly reduced. Compared with chemical fertilizer and chicken manure, sludge promoted N and P absorption of rape more significantly, but the effect of sludge application on K absorption was less significant than chicken manure. Sludge can increase the soil salinity, which is higher than the chemical fertilizer and chicken manure, but the effect was not significant. Compared with chemical fertilizer and chicken manure treatments, the Hg, As and Zn contents in rape plants and soil after sludge application increased, to be specific, Hg content increased relatively significantly. Hg, As and Zn contents in rape plants and soil were all in accordance with China National Safety Standards for Heavy Met- als in Vegetables and China National Environmental Quality Standards for Soils. [Conclusion] Short-term sludge application will not lead to secondary heavy metal pollution of agricultural soils, but the Hg content should be monitored and controlled in long-term sludge application.展开更多
[Objective] This study aimed at investigating the effects of consumption of potassium fertilizer on production and biological characteristics of rape and utilizing efficiency of potassium fertilizer.[Method] Taking 0 ...[Objective] This study aimed at investigating the effects of consumption of potassium fertilizer on production and biological characteristics of rape and utilizing efficiency of potassium fertilizer.[Method] Taking 0 kg/hm2 as control,nine consumptions of potassium fertilizer were designed for the experiment using single factor randomized block design with three replications and field experiment in nine rape-producing areas with different ecological types in Yunnan Province.[Result] Appropriately applying potassium fertilizer in Yunnan Province could improve the rapeseed production,the rape production could achieve 3 983-4 151 kg when applied 150-180 kg/hm2 of K2O,which had increased by 4.7-9.1% compared with the control and attained the peak in K165 treatment.When applied 1 kg of K2O,the rapeseed production could enhance 559 kg,the net profit could reach 1 229 yuan/hm2 and the partial productivity of potassium fertilizer could attain 31.32 kg/kg K2O and 3.7 kg/kg K2O,respectively.Applying potassium fertilizer could promote the growth of rape and increase rapeseed production.[Conclusion] The recommended optimal consumptions of potassium fertilizer with high production and high efficiency for rape in Yunnan Province were ranged between 150 and 180 kg K2O/hm2.However,comprehensively considering production and efficiency factors,the consumption of potassium fertilizer in Yunnan Province was around 73.9 kg/hm2,which should be appropriately increased to around 100.0 kg/hm2 in Longchuan,Yulong,Linxiang and other producing areas with similar conditions.展开更多
[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA...[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA)contents in plant organs and xylem saps were investigated through hydroponic experiment.Four treatments including seed soaking with 0,25,50 and 75 mg/L ALA solutions(CK,A1,A2 and A3)for 6 h were set in the test.[Result] In addition to A3 treatment which made the dry weights of oilseed rape seedlings slightly decrease,after seed soaking with ALA,dry weights,net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of plants significantly increased compared with control.ABA concentrations in plant shoots and xylem saps increased in different levels after seed soaking with ALA.[Conclusion] The increasing endogenous ABA contents might be an explanation for promotion effect of ALA application on the growth of winter oilseed rape seedling.展开更多
[Objective] In order to seek the way of high quality and high yield of oilseed rape.[Method] Soil culture experiment was used in this research.1% (mass fraction) KCl solution was sprayed to the stems and leaves of o...[Objective] In order to seek the way of high quality and high yield of oilseed rape.[Method] Soil culture experiment was used in this research.1% (mass fraction) KCl solution was sprayed to the stems and leaves of oilseed rape in full blooming stage while 1.17% (mass fraction) K2SO4 solution and clear water were used for comparison,sampling in initial silique stage and harvest stage were conducted to detect photosynthate,soluble sugar and free amino acids content.[Result] The results showed that compared with spraying clear water,spraying KCl solution increased the contents of chlorophyll a in functional leaves,the contents of free amino acids and soluble sugar in leaves,stems and silique,and the total amounts of them all in initial silique stage but decreased the contents of free amino acids and soluble sugar in leaves and stems,and the total amounts of them all in harvesting stage,increased the average grain yield 10.20%,increased the average contents of oil,oleinic acid and linoleic acid in rapeseed 4.40%,10.60% and 11.40% respectively,decreased the content of protein,erucic acid and glucosinolate in rapeseed 5.10%,9.70% and 3.70% respectively.No significant difference between spraying K2SO4 solution and spraying clear water,significant difference between them all and spraying KCl solution.[Conclusion] The experimental results provided theoretical basis for increasing oilseed rape yield and quality researches.展开更多
[Objective] The aim of this work was to analyze the N fertilization on the vegetative growth and N uptake of different winter rapeseed varieties at wintering stage. [Method] In two consecutive years (2009-2011), two...[Objective] The aim of this work was to analyze the N fertilization on the vegetative growth and N uptake of different winter rapeseed varieties at wintering stage. [Method] In two consecutive years (2009-2011), two winter rapeseed varieties (B. napus L.), an early maturity variety Zhongyou 116 (ZY116) and a middle-late application maturity variety Zhongyouza 12 (ZYZ12) were employed. Field experiments with different N levels (0, 90, 180, 270, 360 kg N/hm 2 ) were designed. At the wintering stage, the dry matter weight, the nitrogen content and concentration of plants, leaf nitrate reductase activity (NRA) and seed yields were investigated. [Result] The shoot dry matter of ZY116 increased rapidly when N rate ranged from 0 to 180 kg/hm 2 , and it raised slightly when N rate ranged from 180 to 360 kg/hm 2 . The shoot dry matter of ZYZ12 were changed in a single peak curve; the peak of shoot dry matter appeared at 270 kg N/hm 2 . The N concentration and N content in shoot and root increased rapidly when the N rate changed from 90 to 180 kg/hm 2 . Moreover, the N concentration and N content root of in ZYZ12 were much higher than that of ZY116. Present study revealed that the changed trend of leaf nitrate reductase activities (NRA) were significantly increased at the N rate of 180 kg/hm 2 in ZY116 and ZYZ12 compared with the N rate of 90 kg/hm 2 in two years. [Conclusion] Optimal nitrogen application significantly increased the dry weights and N uptake at wintering stage as well as increasing the yield of winter oilseed rape.展开更多
文摘[Objectives] This study was conducted to explore the effects of different N application rates and densities on the growth and development of direct-seeding rape as well as on its yield.[Methods] A field experiment was carried out by setting five N fertilizer treatments and three density levels.[Results] Increasing seeding density and N application rate could improve the seed yield of rape. The suitable N application rate for the three densities were calculated using the fitted fertilizer efficiency models, respectively, to be 186.77, 221.35 and 236.14 kg/hm^2, at which the yields were the highest. The results showed that in this area, the seeding density of direct-seeding rape could be selected in the range of 6.0×10^5-7.5×10^5 plants/hm^2, and the suitable N application rate could be in the range of 221.35-236.14 kg/hm^2.[Conclusions] This study provides a theoretical basis for the maximization of spatial resource and efficient utilization of fertilizer.
基金Supported by National Natural Science Foundation of China(31371562,31301276)Special Fund for Fundamental Scientific Research Business of Central Public Research Institutes(Agriculture)(201103003+2 种基金201203079)Key Projects in the National Science&Technology Pillar Program during the 12thFive-year Plan Period(2012BAD04B08)Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province(CX(14)5021)~~
文摘[Objective] This study aimed to investigate the effect of direct-seeding with non-flooding and wheat residue returning patterns on greenhouse gas emission from rice paddy. [Method] Two rice cultivars currently used in the production, Yangdao 6 (an indica) and Yangjing 4038 (a japonica), were field grown using a direct-seeding method, and four treatments, wheat straw incorporation into soil and traditional flooding (SlF), non-flooding and wheat straw mulching (NSM), non-flooding and wheat straw incorporation into soil (NSl) and traditional flooding (no straw returned, Control, TF), were imposed after sowing to maturity. Effects of direct-seeding with non-flooding and wheat residue returning patterns on CH4, N20 and CO2 emissions were investigated by using the method of static chamber-gas chromatographic tech- niques. [Result] Grain yield showed no significant difference between non-flooding and flooding treatments, but was significantly higher under the SlF than under any other treatments. The emission flux of CH4 and CO2 under TF and SlF exhibited a single peak curve, while changed little under the NSl and NSM The emission flux of N2Oshowed multiple perk curves for all the treatments. Compared with TF, SlF significantly increased mean emission flux of CH4 or N2O, decreased emission of N20, while NSl and NSM significantly decreased the mean emission flux of OH4, and increased emission flux of N2O and CO2. SIF also increased Green Warm Potential (GWP) of CH4, N2O and CO2 and the GWP per unit grain yield by 47.3%- 53.7% and 32.2%-39.4%, respectively. Both NSl and NSM decreased GWP by 24.2%-29.6% and 30.1%-35.5%, and the GWP per unit grain yield was decreased by 21.7-27.2% and 25.6%-31.1%, respectively. [Conelusion] both NSl and NSM could significantly reduce greenhouse effect of CH4, N2O and CO2 meanwhile maintain a high grain yield.
基金supported by the National Natural Science Foundation of China(32102297 and 32272634)。
文摘Plant-mediated RNA interference(RNAi)has emerged as a promising technology for insect control.The green peach aphid,Myzus persicae,feeds on over 400 species of host plants.Brassica napus(rape)is the second most important oilseed crop worldwide.Myzus persicae is highly reproductive and causes severe damage to the rape plants due to its quite flexible life cycle.In this study,we tested the RNAi effects of transgenic rape plants on M.persicae.By in vitro feeding M.persicae with artificial diets containing double-stranded RNAs(dsRNAs)targeting seven aphid genes,we identified a new gene encoding the partitioning-defective protein 6(Par6)as the most potent RNAi target.Tissue-and stage-expression analysis of Par6 suggested this gene is highly expressed in the embryo and adult stage of M.persicae.We next generated transgenic rape plants expressing ds Par6 by Agrobacteriummediated transformation and obtained nine independent transgenic lines.Compared to wild-type control plants,transgenic rape lines expressing ds Par6 showed strong resistance to M.persicae.Feeding assays revealed that feeding transgenic rape plants to M.persicae significantly decreased MpPar6 expression and survival rate and impaired fecundity.Furthermore,we showed that the resistance levels to M.persicae are positively correlated with ds Par6 expression levels in transgenic rape plants.Our study demonstrates that transgenic rape plants expressing ds Par6 are efficiently protected from M.persicae.Interfering with the genes involved in embryo development could be the effective RNAi targets for controlling aphids and potentially other insect pests.
基金supported by the Indian Council of Agricultural Research-International Rice Research Institute Collaborative Project, India (Grant No. OXX4928)。
文摘The sustainability of rice production continues to be a subject of uncertainty and inquiry attributed to shifts in climatic conditions. In light of the impending climate change crisis and the high labor and water costs accompanying it, direct-seeded rice(DSR) is unquestionably one of the most practical solutions. Despite its resource and climate-friendly advantages, early maturing rice faces weed competitiveness and seedling establishment challenges. Resolving these issues is crucial for promoting its wider adoption among farmers, presenting it as a more effective sustainable rice cultivation method globally. Diverse traditional and contemporary breeding methods are employed to mitigate the limitations of the DSR approach, leveraging advanced techniques such as speed breeding and genome editing. Focusing on key traits like mesocotyl length elongation, early seedling vigor, root system architecture, and weed competitiveness holds promise for transformative improvements in DSR adaptation at a broader scale within farming communities. This review aims to summarize how these features contribute to increased crop production in DSR conditions and explore the research efforts focusing on enhancing DSR adaptation through these traits. Emphasizing the pivotal role of these game-changing traits in DSR adaptation, our analysis sheds light on their potential transformative impact and offers valuable insights for advancing DSR practices.
基金the National Nature Science Foundation of China(31770437)。
文摘The cold regions are located in high latitudes and cold climates.The local excellent ornamental plant resources are relatively scarce.The plant species that are suitable for both ornamental and productive benefits of landscape of flower sea construction are also even fewer.Therefore,it is imperative to introduce and screen the plant resources suitable for cold regions to create the landscape of flower sea.The rape,an oilseed crop,was used as a research object in order to create a productive flower landscape with both ornamental and economic values in cold regions.Four rape flower varieties,Qingza No.5,7,9,and 11,were introduced from Qinghai Hufeng Agricultural Science and Technology Group Co.,Ltd.They were planted in the experimental practice base of Northeast Agricultural University in three batches.Development characteristics and seed yield of rape flowers on different sowing dates were studied.The fuzzy probability method was used to comprehensively evaluate the varieties.The results showed that the rape flowers grew well in Harbin City during the experimental sowing period,which could form a good landscape of flower sea and had a considerable rapeseed yield.It could be widely used in cold urban and rural areas,such as Harbin City.In view of the experimental results,the strategies of creating a productive landscape of rape flower sea were proposed and the economic benefits were analyzed.It could change the status quo of a uniform landscape of flower sea in cold regions,help the development of rural tourism,and promote local economic income.
文摘Field experiments were carried out to study the effect of different foliar fertilizer treatments on the agronomic traits,yield,and quality of Brassica napus L.for both vegetable and oil dual-use rape.The seedlings of‘Youtai 929’,‘Jinyoutai 1’,and‘Zhongshuang 11’were sprayed with potassium dihydrogen phosphate,naphthylacetic acid,and selenium fertilizer,and the agronomic traits,yield,and quality were determined.The results showed that the naphthylacetic acid treatment had the highest indexes before winter,followed by the selenium fertilizer treatment.At the harvesting stage,the plant height,number of siliquae,yield per plant,and number of effective branches on the main stem of the three varieties were the highest in the naphthylacetic acid treatment,followed by those in the potassium dihydrogen phosphate treatment.The fresh shoot quality was the best in the selenium fertilizer treatment and the second in the naphthylacetic acid treatment.‘Jinyoutai 1’applied with naphthylacetic acid had the highest economic benefit of 4971.78 CNY/667m^(2),which was 642.32 CNY/667m^(2) higher than that of the control.The findings suggested that naphthylacetic acid was suitable to be used as the foliar fertilizer for the seedlings of B.napus varieties for both vegetable and oilseed,and the additional application of selenium fertilizer demonstrated better effect.
基金financially supported by the National Natural Science Foundation of China(32201868 and 32001575)。
文摘Phosphorus(P)is essential for living plants,and P deficiency is one of the key factors limiting the yield in rapeseed production worldwide.As the most important organ for plants,root morphology traits(RMTs)play a key role in P absorption.To investigate the genetic variability of RMT under low P availability,we dissected the genetic structure of RMTs by genome-wide association studies(GWAS),linkage mapping and candidate gene association studies(CGAS).A total of 52 suggestive loci were associated with RMTs under P stress conditions in 405 oilseed rape accessions.The purple acid phosphatase gene BnPAP17 was found to control the lateral root number(LRN)and root dry weight(RDW)under low P stress.The expression of BnPAP17 was increased in shoot tissue in P-efficient cultivars compared to root tissue and P-inefficient cultivars in response to low P stress.Moreover,the haplotype of BnPAP17^(Hap3)was detected for the selective breeding of P efficiency in oilseed rape.Over-expression of the BnPAP17^(Hap3)could promote the shoot and root growth with enhanced tolerance to low P stress and organic phosphorus(Po)utilization in oilseed rape.Collectively,these findings increase our understanding of the mechanisms underlying BnPAP17-mediated low P stress tolerance in oilseed rape.
基金Supported by the"11th Five-Year Plan"Significant Key Program of Guizhou Province[Guizhou Technology and Agriculture Co-word(2000)1109]Graduate Student Innovation Fund Project of Guizhou University[(2006)009]~~
文摘[Objective] The aim of the research was to find the optimal nitrogen application rate, density and seedling age for no-tillage rape in seedling stage. [Method] With the D-optimal quadratic regression design for three factors, the 310 scheme was designed to study the effects of nitrogen application rate, density and seedling age on dry matter accumulation of no-tillage rape in seedling stage. [Result] With the increase of nitrogen application rate, density and seedling age, the dry matter content appeared like a parabola, increasing firstly and then declining. The change of nitrogen application rate caused greater influence than that of density and seedling age; the interaction effects between nitrogen application rate and density were greater than that between nitrogen application rate and seedling age as well as between density and seedling age. [Conclusion] Considered comprehensively, the dry matter content of no-tillage rape in seedling stage reached the highest level (4 768.2 kg/hm2) when the nitrogen application rate, the density and the seedling age were 195 kg/hm2, 93 000 plants/hm2 and 33 d, respectively.
基金Supported by the Special Fund for Crop Breeding of Sichuan Provincial Department of Education,China (2006LD006)the Rapeseed Breeding Research Program of Science & Technology Department of Sichuan Province,China (2006YZGG-5-5)~~
文摘[Objective] To study the correlation between the biomechanical properties of rape stalks and rape stem lodging. [Method] Through axial compression tests to the stalks of 4 different rape varieties, the change rules of maximum stem bearing ca- pacity, maximum compressive strength, elastic modulus and moment of inertia along plant height were analyzed, as well as the effect of different varieties and water contents on the biomechanical property indices of rape stalks. [Result] The maximum loads of rape stalks presented liner decrease trend along with the increase of stem height, and all reached the maximums below the height of 50 cm. The maximum stem compressive strength and elastic modulus of the 4 varieties were increased with ascending height, but in a slow rate with small change, thus the modulus of e- lasticity could be considered as unchanged. The maximum bearing capacity, maxi- mum compressive strength and elastic modulus of dry rape stalks were higher than wet stalks, indicating that the water contents of rape stalks had significant effect on their mechanical properties. According to the actual lodging situations in filed, stalks of variety No. 1 owned the worst biomechanical properties and lodging degree, while the biomechanical properties of No. 6 and F5 were better than No. 1 and No. 9, and they also had stronger lodging-resistance. [Conclusion] The study provides parameters and bases for the design of mechanized production and mechanical deep processing of crops, and can better reveal the physical natures of organisms. The methods used in this study can also be used to screen excellent crop stalks.
基金Supported by the Special Fund for the Industrial Technology System Construction of Modern Agriculture(nycytx-00563)Department of Science and Technology of Guizhou Province[QinkeheNY(2010)3087]+2 种基金Department of Agriculture of Guizhou Province[Qinnongyu(2009)007]Guizhou Academy of Agricultural Sciences[ZX(2007)015]Special Fund of Guizhou Academy of Agricultural sciences[Qinnongke(2009)030]~~
文摘[Objective] This study aimed to investigate the correlation between yield and quality traits of rape. [Method] Taking "Sanbei 98" and "Youyan 599" as ex- periment materials, correlation between yield and quality traits of Brassica napus L. cultivars was explored by orthogonal rotation method. [Result] The results showed that: 1 In both the two varieties-Sanbei98 and Youyan599, erucic acid content had the largest coefficient of variation, but its average content was lower than 2% in both the two varieties (0.90% in Sanbei98 and 1.24% in Youyan599), and the palmitic acid content had the smallest coefficient of variation. 2 In terms of the dif- ferences in quality traits at different yield levels, only the mean of eicosenoic acid content revealed significant difference in Sanbei98, and only the mean of seed-pro- tein content revealed significant difference in Youyan599, and there was no signifi- cant difference in any combined quality traits in both the two varieties. 3 The yield was negatively correlated with the erucic acid content or oil content, and posi- tively correlated with the content of seed-protein, linoleic acid, or linolenic acid, and the coefficient of correlation between the yield and either of the contents of glucosi- nolates, oleic acid, palmitic acid, stearic acid, and eicosenoic acid was smaller. ~, when the yield of a variety increased considerably, there was a certain decreasing trend in erucic acid content, oil content, the total amount of erucic chain fatty acids (El & Er), while there was an increasing trend in seed-protein content, linoleic acid content, linolenic acid content, saturated fatty acids (SFA), the total amount of fat and protein (P & F), unsaturation index (IUFA), unsaturated fatty acids (UFA), the total amount of 18 carbon fatty acids (18-C FA), unsaturated fatty acids [UFA/(Ei & Er) ], and the sum of oleic acid and linoleic acid (OI & Li). 5 When the seed-pro- tein content is increased, the quality of rape oil cake is also improved; when the unsaturated index is increased, the oil stability is decreased. The increase in the total amount of unsaturated fatty acids, oleic acid and linoleic acid helps to increase in the total amount of fatty acids absorbed by human bodies. [Conclusion] This study provides a theoretical foundation for the cultivation of high-yielding and top- quality hybrid rape cultivars.
基金Supported by Special Funds for Public Welfare Industry(Agricul-ture)Study of China(200903003)Subjects of Special Funds of Public Welfare Institutes of China(1610172011016)~~
文摘[Objective]The paper was to investigate the dynamics of essential metal elements(Ca,Mg,Fe,Mn,Zn and Cu)contents in winter oilseed rape(Brassica napus L.)during cold acclimation,and to reveal the effects and mechanisms of essential elements in cold resistance of oilseed rape.[Method]Three varieties with different cold resistance including Zhongshuang No.11(ZS,freezing sensitive variety),Ganyouza No.1(GY)and Jinyuyou No.1(JYY,freezing tolerant varieties)were used in the study,and the changes of necessary metal elements in these oilseed rapes during cold acclimation were studied.[Result]The concentration of metal elements in oilseed rape had significant changes during cold acclimation,and the difference between varieties was significant.In plant roots,the concentrations of Ca,Mg,Fe,Mn,Zn and Cu were significantly increased,this might because that low temperature made the transpiration decreased,thus blocking the upward transport of elements.In plant shoots,the concentrations of Ca,Mg and Zn were significantly decreased,while the concentrations of Fe and Mn were significantly increased;the change extent of various elements in ZS variety was the largest.[Conclusion]Maintaining the stability of Ca,Mg and Zn concentrations and increasing Fe,Mn and Cu concentrations in plant shoots might be benefit for increasing the cold tolerance of winter oilseed rape.
基金Funds for Transformation of Scientific and Technological Achievements of Ministry of Science and Technology of China (04EFN215200268)the Nomarch Special Foundation for the Excellent Science and Technology Talents of Guizhou Province[(2005(77)]the Science and Technology Program of Guizhou Province[(2006)6001]~~
文摘[ Objective] The aim of the research was to provide reference for reasonable application of nitrogen fertilizer for high yield.cultivation of hybrid rape cuhivar Youyan 9 and Youyan 10. [ Method] The net increment changes of individual plant fresh weight and dry matter weight of Youyan 9 and Youyan 10 with different nitrogen application treatments were studied. [ Result] The differences among average fresh weight increments of individual plant and average dry matter weight increment of individual plant with different treatments reached 0. 01 extremely significant level. Fresh weight increment and dry matter weight net increment of individual plant declined gradually with the increase of nitrogen application. In growtheourse ,fresh weight net increment of individual plant increased firstly then decreased and the maximum was in beginning flowering stage, besides that dry matter net increment increased gradually and the maximum was in mature period. The correlations among fresh net increment, dry matter weight net increment and yield net increment were positive or extremely positive. [ Conclusion] Under experimental condition, when nitrogen application was 225 kg/hm^2, hybrid rape Yanyou 9 and Yanyou 10 with low erucic,low glucosinolate could obtain high yield.
基金Supported by Scientific and Technological Support Project for Quality and Safety Testing and Monitoring of Bulk Agricultural Products Produced in Beijing(Z090905-01040901)Cooperation Project of Beijing Drainage Group Co.Ltd."Study on Agronomic and Environmental Effects of Long-term Application of Sludge"~~
文摘[Objective] This study aimed to provide reliable theoretical basis for re- source utilization of sludge. [Method] By using pot experiment, variations of the yield index and salt content of rape and the accumulation of three kinds of heavy metals in rape plants and soil including Hg, As and Zn were explored. [Result] The results showed that medium sludge level (50 g/pot) significantly increased the rape yield and root/shoot ratio; when the application amount of sludge reached 100 g/pot, the fertil- ization effect of sludge on potted rape yield was significantly reduced. Compared with chemical fertilizer and chicken manure, sludge promoted N and P absorption of rape more significantly, but the effect of sludge application on K absorption was less significant than chicken manure. Sludge can increase the soil salinity, which is higher than the chemical fertilizer and chicken manure, but the effect was not significant. Compared with chemical fertilizer and chicken manure treatments, the Hg, As and Zn contents in rape plants and soil after sludge application increased, to be specific, Hg content increased relatively significantly. Hg, As and Zn contents in rape plants and soil were all in accordance with China National Safety Standards for Heavy Met- als in Vegetables and China National Environmental Quality Standards for Soils. [Conclusion] Short-term sludge application will not lead to secondary heavy metal pollution of agricultural soils, but the Hg content should be monitored and controlled in long-term sludge application.
基金Supported by the National Science and Technology Support Program(2009BADA8B01)Kunming Comprehensive Experimental Station,National Modern Agricultural Industrial Technology System of Rape(NYCYTX-00564)Yunnan Modern Agricultural Industrial Technology System Construction of Rape~~
文摘[Objective] This study aimed at investigating the effects of consumption of potassium fertilizer on production and biological characteristics of rape and utilizing efficiency of potassium fertilizer.[Method] Taking 0 kg/hm2 as control,nine consumptions of potassium fertilizer were designed for the experiment using single factor randomized block design with three replications and field experiment in nine rape-producing areas with different ecological types in Yunnan Province.[Result] Appropriately applying potassium fertilizer in Yunnan Province could improve the rapeseed production,the rape production could achieve 3 983-4 151 kg when applied 150-180 kg/hm2 of K2O,which had increased by 4.7-9.1% compared with the control and attained the peak in K165 treatment.When applied 1 kg of K2O,the rapeseed production could enhance 559 kg,the net profit could reach 1 229 yuan/hm2 and the partial productivity of potassium fertilizer could attain 31.32 kg/kg K2O and 3.7 kg/kg K2O,respectively.Applying potassium fertilizer could promote the growth of rape and increase rapeseed production.[Conclusion] The recommended optimal consumptions of potassium fertilizer with high production and high efficiency for rape in Yunnan Province were ranged between 150 and 180 kg K2O/hm2.However,comprehensively considering production and efficiency factors,the consumption of potassium fertilizer in Yunnan Province was around 73.9 kg/hm2,which should be appropriately increased to around 100.0 kg/hm2 in Longchuan,Yulong,Linxiang and other producing areas with similar conditions.
基金Supported by the Special Funds of Scientific and Technological Support Project of China(2009BADA8B01)Special Funds for Public Welfare Industry(Agriculture)Study of China(200903003)~~
文摘[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA)contents in plant organs and xylem saps were investigated through hydroponic experiment.Four treatments including seed soaking with 0,25,50 and 75 mg/L ALA solutions(CK,A1,A2 and A3)for 6 h were set in the test.[Result] In addition to A3 treatment which made the dry weights of oilseed rape seedlings slightly decrease,after seed soaking with ALA,dry weights,net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of plants significantly increased compared with control.ABA concentrations in plant shoots and xylem saps increased in different levels after seed soaking with ALA.[Conclusion] The increasing endogenous ABA contents might be an explanation for promotion effect of ALA application on the growth of winter oilseed rape seedling.
基金Supported by National Natural Science Foundation of China(30971860 )The Industry Technology System of Rapseed Construction Projects in China (nyctx-00509)+1 种基金Major projects in Hunan Province (2009FJ1006-1 and 2009FJ1006-3)Open Foundation of Innovation Platform in Hunan College(09K049)~~
文摘[Objective] In order to seek the way of high quality and high yield of oilseed rape.[Method] Soil culture experiment was used in this research.1% (mass fraction) KCl solution was sprayed to the stems and leaves of oilseed rape in full blooming stage while 1.17% (mass fraction) K2SO4 solution and clear water were used for comparison,sampling in initial silique stage and harvest stage were conducted to detect photosynthate,soluble sugar and free amino acids content.[Result] The results showed that compared with spraying clear water,spraying KCl solution increased the contents of chlorophyll a in functional leaves,the contents of free amino acids and soluble sugar in leaves,stems and silique,and the total amounts of them all in initial silique stage but decreased the contents of free amino acids and soluble sugar in leaves and stems,and the total amounts of them all in harvesting stage,increased the average grain yield 10.20%,increased the average contents of oil,oleinic acid and linoleic acid in rapeseed 4.40%,10.60% and 11.40% respectively,decreased the content of protein,erucic acid and glucosinolate in rapeseed 5.10%,9.70% and 3.70% respectively.No significant difference between spraying K2SO4 solution and spraying clear water,significant difference between them all and spraying KCl solution.[Conclusion] The experimental results provided theoretical basis for increasing oilseed rape yield and quality researches.
基金Supported by the Special Funds for Modern Agricultural (oilseed rape) Technical System (MATS) of Chinathe National Natural Science Foundation of China (NSFC) (31071372)~~
文摘[Objective] The aim of this work was to analyze the N fertilization on the vegetative growth and N uptake of different winter rapeseed varieties at wintering stage. [Method] In two consecutive years (2009-2011), two winter rapeseed varieties (B. napus L.), an early maturity variety Zhongyou 116 (ZY116) and a middle-late application maturity variety Zhongyouza 12 (ZYZ12) were employed. Field experiments with different N levels (0, 90, 180, 270, 360 kg N/hm 2 ) were designed. At the wintering stage, the dry matter weight, the nitrogen content and concentration of plants, leaf nitrate reductase activity (NRA) and seed yields were investigated. [Result] The shoot dry matter of ZY116 increased rapidly when N rate ranged from 0 to 180 kg/hm 2 , and it raised slightly when N rate ranged from 180 to 360 kg/hm 2 . The shoot dry matter of ZYZ12 were changed in a single peak curve; the peak of shoot dry matter appeared at 270 kg N/hm 2 . The N concentration and N content in shoot and root increased rapidly when the N rate changed from 90 to 180 kg/hm 2 . Moreover, the N concentration and N content root of in ZYZ12 were much higher than that of ZY116. Present study revealed that the changed trend of leaf nitrate reductase activities (NRA) were significantly increased at the N rate of 180 kg/hm 2 in ZY116 and ZYZ12 compared with the N rate of 90 kg/hm 2 in two years. [Conclusion] Optimal nitrogen application significantly increased the dry weights and N uptake at wintering stage as well as increasing the yield of winter oilseed rape.