High-Imperceptibility Data Hiding Scheme for JPEG Images Based on Direction Modification

Data hiding(DH)is an important technology for securely transmitting secret data in networks,and has increasing become a research hotspot throughout the world.However,for Joint photographic experts group(JPEG)images,it is difficult to balance the contradiction among embedded capacity,visual quality and the file size increment in existing data hiding schemes.Thus,to deal with this problem,a high-imperceptibility data hiding for JPEG images is proposed based on direction modification.First,this proposed scheme sorts all of the quantized discrete cosine transform(DCT)block in ascending order according to the number of non-consecutive-zero alternating current(AC)coefficients.Then it selects non-consecutive-zero AC coefficients with absolute values less than or equal to 1 at the same frequency position in two adjacent blocks for pairing.Finally,the 2-bit secret data can be embedded into a coefficient-pair by using the filled reference matrix and the designed direction modification rules.The experiment was conducted on 5 standard test images and 1000 images of BOSSbase dataset,respectively.The experimental results showed that the visual quality of the proposed scheme was improved by 1∼4 dB compared with the comparison schemes,and the file size increment was reduced at most to 15%of the comparison schemes.

Data-Hiding Based on Sudoku and Generalized Exploiting Modification Direction

Division arithmetic and generalized exploiting modification direction （DA-GEMD） method has perfect efficiency in data-hiding. However, there exist some weaknesses in DA-GEMD. Because the benchmarks are their median of nine pixels in each block of cover-image, it will give a hint for the attacker to hack the stego-image. In the paper, we utilize the Sudoku table to randomize the benchmark for each 3×3 block of cover-image. It will mix up the pixels in each image block and prevent the secret information from being extracted. The proposed method still keeps the original efficiency as DA-GEMD while promoting the security for data-hiding.

Using multiple site-directed modification of epoxide hydrolase to significantly improve its enantioselectivity in hydrolysis of rac-glycidyl phenyl ether

The epoxide hydrolase gene(SpEH) from Sphingomonas sp. HXN-200 was synthesized and expressed in robust Escherichia coli cells that had a dual protection system. The enantioselectivity(E-value) of the recombinant SpEH was 7.7 and the yield of the remaining(R)-PGE was 24.3% for the hydrolysis of racemic phenyl glycidyl ether(rac-PGE). To improve the catalytic properties of SpEH, the site-directed mutagenesis was carried out based on homology modeling, sequence alignment and molecular docking. Six residues(V195, V196, F218,N226, Q312, and M332) near the active site were mutated to hydrophobic amino acids and the positive mutations were selected for combinatorial mutation. The optimal mutant SpEH^(V196A/N226A/M332A) had an enhanced E-value of 21.2 and a specific activity of 4.57 U·mg^-1-wet cells, which were 2.8-, and 2.3-fold higher than those of wild-type SpEH. The optimal temperature and p H for purified Sp EHV196 A/N226 A/M332 Ato catalyze the hydrolysis of rac-PGE were 25 ℃ and 7.0 with 200 U·mg^-1. The enantioselectivity and yield of the remaining(R)-PGE of E. coliSpEH^(V196A/N226A/M332A)increased from 7.7 to 21.2 and 24.3% to 40.9%, respectively. The molecular docking and kinetic parameter analyses showed that SpEH^(V196A/N226A/M332A) has a greater affinity toward(S)-PGE than(R)-PGE, and that it was more difficult for the O-atom of ASP170 to achieve the nucleophilic attack on the Cα of(R)-PGE, resulting in its improved enantioselectivity.

DIRECT DIGITAL DESIGN AND SIMULATION OF MESHING IN WORM-GEAR DRIVE

A direct digital design method （DDDM） of worm-gear drive is proposed. It is directly based on the simulation of manufacturing process and completely different from the conventional modeling method. The loaded tooth contact analysis （LTCA） method is analyzed, in which the advanced surface to surface searching technique is included. The influence of misalignment errors and contact deformations on contact zone and transmission error （TE） is discussed. Combined modification approach on worm tooth surface is presented. By means of DDDM and LTCA, it is very conven- ient to verify the effect of worm-gear drive＇s modification approach. The analysis results show that, the modification in profile direction reduces the sensitivity of worm-gear drive to misalignment errors and the modification in longitudinal direction decreases the TE. Thus the optimization design of worm-gear drive can be achieved prior to the actual manufacturing process.