Traditional Chinese medicine(TCM)has become very popular in the treatment of complex diseases worldwide in recent decades.Despite the promising performance of TCM,how herbs work synergistically in a formula and their ...Traditional Chinese medicine(TCM)has become very popular in the treatment of complex diseases worldwide in recent decades.Despite the promising performance of TCM,how herbs work synergistically in a formula and their therapeutic targets remain ambiguous.This largely limits the correct use and modernization of TCM formulas clinically.The study of disassembled prescriptions is important for investigating the compatibility theory of Chinese medicines.By dissecting the mechanisms of TCM formulae,the compatibility principles of some well-known formulae,such as Realgar-Indigo naturalis,have been elucidated to a certain extent.In this review,several common methods of disassembled prescription studies are summarized,including those of single herb or mineral,sub-prescription,active ingredients or effective parts,large formulae,and systems pharmacology studies,as well as the methods of grouping based on the properties or efficacy of Chinese medicines,in order to provide evidence for formulae study.Moreover,some challenges to be addressed in disassembled prescriptions studies have been identified in this review.展开更多
Objective:To explore the therapeutic capacity of the Liangxue Xiaoban(LXXB)decoction and its disassembled prescriptions in the modulation of T cell subsets and recurrence-related indexes of psoriasis using a psoriasis...Objective:To explore the therapeutic capacity of the Liangxue Xiaoban(LXXB)decoction and its disassembled prescriptions in the modulation of T cell subsets and recurrence-related indexes of psoriasis using a psoriasis-like mouse model.Methods:The psoriasis model was generated by the treatment of BALB/c mice(n=48)with imiquimod.Mice were divided into six groups:control,psoriasis model,tripterygium glycosides,LXXB decoction,Liangxue decoction,and Qingqi decoction.After the intervention period,the interleukin(IL)-17A,IL-22,and interferon-γ levels in mice were examined and hematoxylin and eosin staining was conducted to determine pathological changes in the skin tissues.T cell subset changes in the skin-draining lymph nodes were analyzed using flow cytometry,and the expression levels of the associated transcription factors and recurrence-related indexes in the skin tissues were determined using a polymerase chain reaction.Results:LXXB decoction attenuated the levels of CD8^(+)T,Th17,and Th1 cells and induced an increase in the Th2 and Treg cell levels.The disassembled prescriptions promoted or inhibited specific subsets of T cells to improve the symptoms of psoriasis.Notably,the LXXB and Liangxue decoctions suppressed the expression of IL-22 at both the gene and protein levels and restored the CD103 and IL-15 expressions in the skin tissue to the normal range.Conclusion:LXXB decoction exerted significant immunoregulatory effects on T cell subsets and improved the recurrence-related indexes.Interestingly,the Liangxue prescription appeared to have a therapeutic advantage in terms of Th17 modulation and psoriasis recurrence,while the Qingqi prescription performed better in Treg immunoregulation.展开更多
Objective:To study the effects of Erlong Zuoci Pill(耳聋左慈丸,ELZCP)and its disassembled prescriptions on gentamicin(GM)-induced ototoxicity model in vitro.Methods:After the spiral organ of cochleae of newborn ...Objective:To study the effects of Erlong Zuoci Pill(耳聋左慈丸,ELZCP)and its disassembled prescriptions on gentamicin(GM)-induced ototoxicity model in vitro.Methods:After the spiral organ of cochleae of newborn mice(postnatal days:2-3)cultured for 24 h,GM alone or combined with water extracting-alcohol precipitating solution of ELZCP or with its disassembled prescriptions was added.Hair cells were observed under a fluorescence microscope after TRITC-phalloidin staining,and the cochlear hair cell loss rate was calculated by counting the whole cochlear hair cells and analyzed by whole cochlear hair cells analyzing software.Results: GM induced cochlear outer hair cells(OHCs)and inner hair cells(IHCs)injuries in a dose-dependent manner, and they were significantly different as compared with those in the normal control group(P〈0.05,P〈0.01). ELZCP at the concentration of 0.003-3 mg/mL could decrease the hair cells loss induced by the 0.3 mmol/L GM (P〈0.05,P〈0.01),the effects was in a dose-dependent manner,and the concentration of 0.3 mg/mL showed the optimal protective effect.For the ELZCP disassembled prescriptions,Liuwei-Dihuang could decrease OHC loss rate than that in the 0.3 mmol/L GM model group(P〈0.05),but the OHC loss rate was still higher than that in the ELZCP group(P〈0.01),which indicated that the protective effect of hair cells by Liuwei-Dihuang was not better than that of ELZCP.Poria decreased OHC loss rate from 72.1%±3.7%to 58.8%±8.2%(P〈0.05). Conclusions:ELZCP could play a role in antagonizing the injury of cochlear hair cells induced by GM ototoxicity, and its disassembled prescriptions,Liuwei-Dihuang was the main component to protect the cochlear hair cells from GM-induced ototoxicity,and Magnetitum combined with Radix Bupleurui could strengthen the action of the whole prescription; Poria could reduce GM-induced OHC loss.展开更多
The hybrid Janus nanomaterials have captured considerable attention since the asymmetric structure can combine the properties of each component and display synergistic applications.However,the precise design of the sp...The hybrid Janus nanomaterials have captured considerable attention since the asymmetric structure can combine the properties of each component and display synergistic applications.However,the precise design of the specific hybrid Janus nanostructures still remains a formidable challenge.Here,we for the first time report the fabrication of novel and highly uniform inorganic/organic hybrid Janus nanotubes via disassembling the mesoporous inorganic nanoparticles(NPs)/polystyrene-block-poly(4-vinylpyridine)(PS-b-P4VP)hybrid sheets,which are generated through the in situ reduction of functional metal precursors on the prepared PS-b-P4VP mesoporous scaffolds.More importantly,the internal pore size of the hybrid Janus nanotubes can be precisely tuned by readily controlling the swelling time of the PS-b-P4VP sheet-like assemblies in a selective solvent for P4VP domains,thus generating the particular inorganic/organic hybrid Janus nanotubes with adjustable inner diameter size.We believe that our finding will provide an efficient and universal route to fabricate the particular inorganic/organic hybrid nanotubes for hierarchical functional devices and nanomaterials.展开更多
After remanufacturing disassembly,several kinds of friction damages can be found on the mating surface of interference fit.These damages should be repaired and the cost is closely related to the severity of damages.In...After remanufacturing disassembly,several kinds of friction damages can be found on the mating surface of interference fit.These damages should be repaired and the cost is closely related to the severity of damages.Inspired by the excellent performance of surface texture in wear reduction,5 shapes of pit array textures are added to the specimens’surface to study their reduction effect of disassembly damage for interference fit.The results of disassembly experiments show that the order of influence of texture parameters on disassembly damage is as follows:equivalent circle diameter of single texture,texture shape and texture surface density.The influence of equivalent circle diameter of single texture and texture shape are obviously more significant than that of texture surface density.The circular texture with a surface density of 30%and a diameter of 100μm shows an excellent disassembly damage reduction effect because of its perfect ability of abrasive particle collection.And the probability of disassembly damage formation and evolution is also relatively small on this kind of textured surface.Besides,the load-carrying capacity of interference fit with the excellent texture is confirmed by load-carrying capacity experiments.The results show that the load-carrying capacity of the excellent texture surface is increased about 40%compared with that of without texture.This research provides a potential approach to reduce disassembly damage for interference fit.展开更多
Disassembly sequence planning (DSP) plays a significant role in maintenance planning of the aircraft. It is used during the design stage for the analysis of maintainability of the aircraft. To solve product disassem...Disassembly sequence planning (DSP) plays a significant role in maintenance planning of the aircraft. It is used during the design stage for the analysis of maintainability of the aircraft. To solve product disassembly sequence planning problems efficiently, a product disassembly hybrid graph model, which describes the connection, non-connection and precedence relationships between the product parts, is established based on the characteristic of disassembly. Farther, the optimization model is provided to optimize disassembly sequence. And the solution methodology based on the genetic/simulated annealing algorithm with binaxy-tree algorithm is given. Finally, an example is analyzed in detail, and the result shows that the model is correct and efficient.展开更多
The current research of configurable product disassemblability focuses on disassemblability evaluation and disassembly sequence planning. Little work has been done on quantitative analysis of configurable product disa...The current research of configurable product disassemblability focuses on disassemblability evaluation and disassembly sequence planning. Little work has been done on quantitative analysis of configurable product disassemblability. The disassemblability modeling technology for configurable product based on disassembly constraint relation weighted design structure matrix (DSM) is proposed. Major factors affecting the disassemblability of configurable product are analyzed, and the disassembling degrees between components in configurable product are obtained by calculating disassembly entropies such as joint type, joint quantity, disassembly path, disassembly accessibility and material compatibility. The disassembly constraint relation weighted DSM of configurable product is constructed and configuration modules are formed by matrix decomposition and tearing operations. The disassembly constraint relation in configuration modules is strong coupling, and the disassembly constraint relation between modules is weak coupling, and the disassemblability configuration model is constructed based on configuration module. Finally, taking a hydraulic forging press as an example, the decomposed weak coupling components are used as configuration modules alone, components with a strong coupling are aggregated into configuration modules, and the disassembly sequence of components inside configuration modules is optimized by tearing operation. A disassemblability configuration model of the hydraulic forging press is constructed. By researching the disassemblability modeling technology of product configuration design based on disassembly constraint relation weighted DSM, the disassembly property in maintenance, recycling and reuse of configurable product are optimized.展开更多
Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerativ...Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, wheth- er tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/ threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in periph- eral nerve repair and regeneration.展开更多
Statistic and typical-year composition methods are used to study the northwest Pacific typhoonactivities in relation with the El Nino and La Nina events. The result indicates that the typhoon tends to be inactive in t...Statistic and typical-year composition methods are used to study the northwest Pacific typhoonactivities in relation with the El Nino and La Nina events. The result indicates that the typhoon tends to be inactive in the El Nino years and active in the La Nina years and it is also dependent on the onset and ending time andintensity of the events and areas of genesis of typhoons. With statistic features of the frequency of typhoon activity in the EI Nino and La Nina years and the time-lag correlation between the frequency and sea surface temperature (SST), useful information is provided for the prediction of typhoon occurrence. In addition, the singularvalues disassemble (SVD) method is applied to study the correlation between the geopotential field and SST fief(l.The result shows that the air-sea coupling in the EI Nino years is unfavorable for the typhoon to develop. whichtake place with a smaller number. Opposite situations are found with the La Nina years.展开更多
It is well-recognized that obsolete or discarded products can cause serious environmental pollution if they are poorly be handled.They contain reusable resource that can be recycled and used to generate desired econom...It is well-recognized that obsolete or discarded products can cause serious environmental pollution if they are poorly be handled.They contain reusable resource that can be recycled and used to generate desired economic benefits.Therefore,performing their efficient disassembly is highly important in green manufacturing and sustainable economic development.Their typical examples are electronic appliances and electromechanical/mechanical products.This paper presents a survey on the state of the art of disassembly sequence planning.It can help new researchers or decision makers to search for the right solution for optimal disassembly planning.It reviews the disassembly theory and methods that are applied for the processing,repair,and maintenance of obsolete/discarded products.This paper discusses the recent progress of disassembly sequencing planning in four major aspects:product disassembly modeling methods,mathematical programming methods,artificial intelligence methods,and uncertainty handling.This survey should stimulate readers to be engaged in the research,development and applications of disassembly and remanufacturing methodologies in the Industry 4.0 era.展开更多
The cooperative work between human being and computer based on virtual reality (VR) is investigated to plan the disassembly sequences more efficiently. A three-layer model of human-computer cooperative virtual disasse...The cooperative work between human being and computer based on virtual reality (VR) is investigated to plan the disassembly sequences more efficiently. A three-layer model of human-computer cooperative virtual disassembly is built, and the corresponding human-computer system for stable virtual disassembly is developed. In this system, an immersive and interactive virtual disassembly environment has been created to provide planners with a more visual working scene. For cooperative disassembly, an intelligent module of stability analysis of disassembly operations is embedded into the human-computer system to assist planners to implement disassembly tasks better. The supporting matrix for stability analysis of disassembly operations is defined and the method of stability analysis is detailed. Based on the approach, the stability of any disassembly operation can be analyzed to instruct the manual virtual disassembly. At last, a disassembly case in the virtual environment is given to prove the validity of above ideas.展开更多
Maintainability is an important design characterist ic , and also is a design parameter. The essence of maintainability design is to ex pect and solve possible maintenance problems at early stage during product devel ...Maintainability is an important design characterist ic , and also is a design parameter. The essence of maintainability design is to ex pect and solve possible maintenance problems at early stage during product devel opment, maintainability must be designed into product simultaneously. But curren t maintainability methods do not support concurrent engineering. This paper anal yzes the deficiencies of existing maintainability technologies, and introduces t he concept of maintainability analysis visualization. The two major deficiencies are that maintainability analysis time lags behind product design, and the main tainability techniques do not accommodate modern product design concepts. After this, the infrastructure of maintainability analysis visualization system is dis cussed. The system are composed of four subsystems, they are product modeling, d isassembly process modeling, simulation and analysis, and user interface. Produc t modeling is accomplished with the capability of CAD system, disassembly proces s modeling is mainly a reason process based on assembly information. The system is integrated into AutoCAD so that designer can timely analyze their product des ign from the viewpoint of easy maintenance. Human model generation function, hum an task analysis function, tool analysis function, visibility analysis function is implemented, disassembly process planning function is still under developing.展开更多
The end-of-life vehicle recycling was studied based on the disassembly. The end-of-life recycling and the disassembly were reviewed and discussed. A disassembly experiment of an end-of-life engine was carried out, whi...The end-of-life vehicle recycling was studied based on the disassembly. The end-of-life recycling and the disassembly were reviewed and discussed. A disassembly experiment of an end-of-life engine was carried out, which strictly recorded the process of dismantling. Based on the results, a model of the end-of-life recycling was presented. In this model, the end-of-life parts were classified by three ways which included to recycle directly, to recycle after remanufacturing and to discard. By using this model, the dismantling efficiency and the recycling rate can be improved. Also, it obtains a good result after used in a dismantling factory.展开更多
The evolution of Industry 4.0 made it essential to adopt the Internet of Things(IoT)and Cloud Computing(CC)technologies to perform activities in the new age of manufacturing.These technologies enable collecting,storin...The evolution of Industry 4.0 made it essential to adopt the Internet of Things(IoT)and Cloud Computing(CC)technologies to perform activities in the new age of manufacturing.These technologies enable collecting,storing,and retrieving essential information from the manufacturing stage.Data collected at sites are shared with others where execution automatedly occurs.The obtained information must be validated at manufacturing to avoid undesirable data losses during the de-manufacturing process.However,information sharing from the assembly level at the manufacturing stage to disassembly at the product end-of-life state is a major concern.The current research validates the information optimally to offer a minimum set of activities to complete the disassembly process.An optimal disassembly sequence plan(DSP)can possess valid information to organize the necessary actions in manufacturing.However,finding an optimal DSP is complex because of its combinatorial nature.The genetic algorithm(GA)is a widely preferred artificial intelligence(AI)algorithm to obtain a near-optimal solution for the DSP problem.The converging nature at local optima is a limitation in the traditional GA.This study improvised the GA workability by integrating with the proposed priori crossover operator.An optimality function is defined to reduce disassembly effort by considering directional changes as parameters.The enhanced GA method is tested on a real-time product to evaluate the performance.The obtained results reveal that diversity control depends on the operators employed in the disassembly attributes.The proposed method’s solution can be stored in the cloud and shared through IoT devices for effective resource allocation and disassembly for maximum recovery of the product.The effectiveness of the proposed enhanced GA method is determined by making a comparative assessment with traditional GA and other AI methods at different population sizes.展开更多
The recycling and remanufacturing of end-of-life products are significant for environmental protection and resource conservation.Disassembly is an essential process of remanufacturing end-of-life products.Effective di...The recycling and remanufacturing of end-of-life products are significant for environmental protection and resource conservation.Disassembly is an essential process of remanufacturing end-of-life products.Effective disassembly plans help improve disassembly efficiency and reduce disassembly costs.This paper studies a disassembly planning problem with operation attributes,in which an integrated decision of the disassembly sequence,disassembly directions,and disassembly tools are made.Besides,a mathematical model is formulated with the objective of minimizing the penalty cost caused by the changing of operation attributes.Then,a neighborhood modularization-based artificial bee colony algorithm is developed,which contains a modular optimized design.Finally,two case studies with different scales and complexities are used to verify the performance of the proposed approach,and experimental results show that the proposed algorithm outperforms the two existing methods within an acceptable computational time.展开更多
Sarl is a small GTPase involved in COPⅡ vesicle transport. Previous studies showed that H79G mutation of Sarl can lock Sarl in its GTP-bound active conformation, stabilize coat assembly, and prevent the disassembly o...Sarl is a small GTPase involved in COPⅡ vesicle transport. Previous studies showed that H79G mutation of Sarl can lock Sarl in its GTP-bound active conformation, stabilize coat assembly, and prevent the disassembly of COP Ⅱ vesicle coats by reducing Sec23/24 GAP- stimulated hydrolysis. We show here that the replacement of His79 by glycine induces a large conformation change in switch Ⅱ and results in the lost of hydrogen bond between His79 and its associated nucleophilic water molecule that was hypothesized to reduce the GAP-stimulated hydrolysis during the COP Ⅱ assembly and disassembly. These results confirm that the switch Ⅱ conformation is important for COP Ⅱ disassembly through coat-controlled GTP hydrolysis.展开更多
Introduction Vascular endothelial (VE)-cadherin is localized to the endothelial borders and the adherens junctions,which are regulated by changes in mitogen activated protein kinases (MAPK),GTPases,and intracellular c...Introduction Vascular endothelial (VE)-cadherin is localized to the endothelial borders and the adherens junctions,which are regulated by changes in mitogen activated protein kinases (MAPK),GTPases,and intracellular calcium. We previously展开更多
When using shape memory materials into active disassembly of actual electronic products, because the elastic modulus of shape memory materials is affected by the temperature is relatively large, therefore, the main di...When using shape memory materials into active disassembly of actual electronic products, because the elastic modulus of shape memory materials is affected by the temperature is relatively large, therefore, the main difference of environmental reliability between active disassembly products and common products is the impact of collision and vibration under different temperature. Establishing three-dimensional analysis model, comparing the impact of collision and vibration of mobile phone shells which are made up of PVC materials after casting & radiation and PC/ABS materials under different temperature. Analyzing the reliability of mobile phone under different temperature and optimizing its structure according to data of testing.展开更多
In order to help designers, consider disassembly in their design activities, a method for evaluation of product disassembly is proposed. Criteria characterizing the disassembility of a product are identified. Each cri...In order to help designers, consider disassembly in their design activities, a method for evaluation of product disassembly is proposed. Criteria characterizing the disassembility of a product are identified. Each criterion is assigned a coefficient, thus proposing an index of easy fixations (Iff) as a design indicator to evaluate the level of disassembly of a solution generated by the designer. This index is calculated as a weighted average of the indicators for evaluating the disassembility of the product. The proposed method uses information such as the list of subsets or parts, the component tree, the part geometry, the functional links between components and parts, the properties of the components. It has been tested on one product: the soy roaster.展开更多
Challenges still remain in carrying out assembly modeling efficiently in virtual assembly (VA) fields. One of the root causes is the apparent weakness in effective description of assembly knowledge and information. ...Challenges still remain in carrying out assembly modeling efficiently in virtual assembly (VA) fields. One of the root causes is the apparent weakness in effective description of assembly knowledge and information. The assembly modeling, disassembly modeling, assembly interference inspection, assembly sequence planning and optimization, and assembly simulation display for key techniques is studied theoretically in this paper. An example of product assembly modeling is provided to illustrate the effectiveness of the proposed approach. On the basis of re- search, using assembly simulation techniques and multimedia techniques to finish structure design in linkage design of a large size wind-drive generator. The application of the modeling method has shortened the lead time dramatically.展开更多
基金the project of Young Technical Talents of Tianjin Medical University General Hospital(GG-2021-08209060209507).
文摘Traditional Chinese medicine(TCM)has become very popular in the treatment of complex diseases worldwide in recent decades.Despite the promising performance of TCM,how herbs work synergistically in a formula and their therapeutic targets remain ambiguous.This largely limits the correct use and modernization of TCM formulas clinically.The study of disassembled prescriptions is important for investigating the compatibility theory of Chinese medicines.By dissecting the mechanisms of TCM formulae,the compatibility principles of some well-known formulae,such as Realgar-Indigo naturalis,have been elucidated to a certain extent.In this review,several common methods of disassembled prescription studies are summarized,including those of single herb or mineral,sub-prescription,active ingredients or effective parts,large formulae,and systems pharmacology studies,as well as the methods of grouping based on the properties or efficacy of Chinese medicines,in order to provide evidence for formulae study.Moreover,some challenges to be addressed in disassembled prescriptions studies have been identified in this review.
基金This experimental study was supported by the Key Research Project of the Beijing University of Chinese Medicine(2020-JYBZDGG-023)Young Qihuang Scholars Project(90020163320001).
文摘Objective:To explore the therapeutic capacity of the Liangxue Xiaoban(LXXB)decoction and its disassembled prescriptions in the modulation of T cell subsets and recurrence-related indexes of psoriasis using a psoriasis-like mouse model.Methods:The psoriasis model was generated by the treatment of BALB/c mice(n=48)with imiquimod.Mice were divided into six groups:control,psoriasis model,tripterygium glycosides,LXXB decoction,Liangxue decoction,and Qingqi decoction.After the intervention period,the interleukin(IL)-17A,IL-22,and interferon-γ levels in mice were examined and hematoxylin and eosin staining was conducted to determine pathological changes in the skin tissues.T cell subset changes in the skin-draining lymph nodes were analyzed using flow cytometry,and the expression levels of the associated transcription factors and recurrence-related indexes in the skin tissues were determined using a polymerase chain reaction.Results:LXXB decoction attenuated the levels of CD8^(+)T,Th17,and Th1 cells and induced an increase in the Th2 and Treg cell levels.The disassembled prescriptions promoted or inhibited specific subsets of T cells to improve the symptoms of psoriasis.Notably,the LXXB and Liangxue decoctions suppressed the expression of IL-22 at both the gene and protein levels and restored the CD103 and IL-15 expressions in the skin tissue to the normal range.Conclusion:LXXB decoction exerted significant immunoregulatory effects on T cell subsets and improved the recurrence-related indexes.Interestingly,the Liangxue prescription appeared to have a therapeutic advantage in terms of Th17 modulation and psoriasis recurrence,while the Qingqi prescription performed better in Treg immunoregulation.
基金Supported by Shanghai Science and Technology Commission (No.04ZR14130)Leading Academic Discipline Project of Shanghai Municipal Education Commission(No.J50301)
文摘Objective:To study the effects of Erlong Zuoci Pill(耳聋左慈丸,ELZCP)and its disassembled prescriptions on gentamicin(GM)-induced ototoxicity model in vitro.Methods:After the spiral organ of cochleae of newborn mice(postnatal days:2-3)cultured for 24 h,GM alone or combined with water extracting-alcohol precipitating solution of ELZCP or with its disassembled prescriptions was added.Hair cells were observed under a fluorescence microscope after TRITC-phalloidin staining,and the cochlear hair cell loss rate was calculated by counting the whole cochlear hair cells and analyzed by whole cochlear hair cells analyzing software.Results: GM induced cochlear outer hair cells(OHCs)and inner hair cells(IHCs)injuries in a dose-dependent manner, and they were significantly different as compared with those in the normal control group(P〈0.05,P〈0.01). ELZCP at the concentration of 0.003-3 mg/mL could decrease the hair cells loss induced by the 0.3 mmol/L GM (P〈0.05,P〈0.01),the effects was in a dose-dependent manner,and the concentration of 0.3 mg/mL showed the optimal protective effect.For the ELZCP disassembled prescriptions,Liuwei-Dihuang could decrease OHC loss rate than that in the 0.3 mmol/L GM model group(P〈0.05),but the OHC loss rate was still higher than that in the ELZCP group(P〈0.01),which indicated that the protective effect of hair cells by Liuwei-Dihuang was not better than that of ELZCP.Poria decreased OHC loss rate from 72.1%±3.7%to 58.8%±8.2%(P〈0.05). Conclusions:ELZCP could play a role in antagonizing the injury of cochlear hair cells induced by GM ototoxicity, and its disassembled prescriptions,Liuwei-Dihuang was the main component to protect the cochlear hair cells from GM-induced ototoxicity,and Magnetitum combined with Radix Bupleurui could strengthen the action of the whole prescription; Poria could reduce GM-induced OHC loss.
基金financially supported by the National Natural Science Foundation of China for General Program(No.22072143)and Major Program(No.51433009)。
文摘The hybrid Janus nanomaterials have captured considerable attention since the asymmetric structure can combine the properties of each component and display synergistic applications.However,the precise design of the specific hybrid Janus nanostructures still remains a formidable challenge.Here,we for the first time report the fabrication of novel and highly uniform inorganic/organic hybrid Janus nanotubes via disassembling the mesoporous inorganic nanoparticles(NPs)/polystyrene-block-poly(4-vinylpyridine)(PS-b-P4VP)hybrid sheets,which are generated through the in situ reduction of functional metal precursors on the prepared PS-b-P4VP mesoporous scaffolds.More importantly,the internal pore size of the hybrid Janus nanotubes can be precisely tuned by readily controlling the swelling time of the PS-b-P4VP sheet-like assemblies in a selective solvent for P4VP domains,thus generating the particular inorganic/organic hybrid Janus nanotubes with adjustable inner diameter size.We believe that our finding will provide an efficient and universal route to fabricate the particular inorganic/organic hybrid nanotubes for hierarchical functional devices and nanomaterials.
基金Supported by National Natural Science Foundation of China (Grant No.51405121)。
文摘After remanufacturing disassembly,several kinds of friction damages can be found on the mating surface of interference fit.These damages should be repaired and the cost is closely related to the severity of damages.Inspired by the excellent performance of surface texture in wear reduction,5 shapes of pit array textures are added to the specimens’surface to study their reduction effect of disassembly damage for interference fit.The results of disassembly experiments show that the order of influence of texture parameters on disassembly damage is as follows:equivalent circle diameter of single texture,texture shape and texture surface density.The influence of equivalent circle diameter of single texture and texture shape are obviously more significant than that of texture surface density.The circular texture with a surface density of 30%and a diameter of 100μm shows an excellent disassembly damage reduction effect because of its perfect ability of abrasive particle collection.And the probability of disassembly damage formation and evolution is also relatively small on this kind of textured surface.Besides,the load-carrying capacity of interference fit with the excellent texture is confirmed by load-carrying capacity experiments.The results show that the load-carrying capacity of the excellent texture surface is increased about 40%compared with that of without texture.This research provides a potential approach to reduce disassembly damage for interference fit.
基金supported by the National High Technology Research and Development Program of China(2006AA04Z427).
文摘Disassembly sequence planning (DSP) plays a significant role in maintenance planning of the aircraft. It is used during the design stage for the analysis of maintainability of the aircraft. To solve product disassembly sequence planning problems efficiently, a product disassembly hybrid graph model, which describes the connection, non-connection and precedence relationships between the product parts, is established based on the characteristic of disassembly. Farther, the optimization model is provided to optimize disassembly sequence. And the solution methodology based on the genetic/simulated annealing algorithm with binaxy-tree algorithm is given. Finally, an example is analyzed in detail, and the result shows that the model is correct and efficient.
基金Supported by National Natural Science Foundation of China(Grant No.51375437)Zhejiang Provincial Natural Science Foundation of China(Grant No.LY12E05019)
文摘The current research of configurable product disassemblability focuses on disassemblability evaluation and disassembly sequence planning. Little work has been done on quantitative analysis of configurable product disassemblability. The disassemblability modeling technology for configurable product based on disassembly constraint relation weighted design structure matrix (DSM) is proposed. Major factors affecting the disassemblability of configurable product are analyzed, and the disassembling degrees between components in configurable product are obtained by calculating disassembly entropies such as joint type, joint quantity, disassembly path, disassembly accessibility and material compatibility. The disassembly constraint relation weighted DSM of configurable product is constructed and configuration modules are formed by matrix decomposition and tearing operations. The disassembly constraint relation in configuration modules is strong coupling, and the disassembly constraint relation between modules is weak coupling, and the disassemblability configuration model is constructed based on configuration module. Finally, taking a hydraulic forging press as an example, the decomposed weak coupling components are used as configuration modules alone, components with a strong coupling are aggregated into configuration modules, and the disassembly sequence of components inside configuration modules is optimized by tearing operation. A disassemblability configuration model of the hydraulic forging press is constructed. By researching the disassemblability modeling technology of product configuration design based on disassembly constraint relation weighted DSM, the disassembly property in maintenance, recycling and reuse of configurable product are optimized.
基金supported by the National Natural Science Foundation of China,No.81130080,31300942the National Key Basic Research Program of China(973 Program)+5 种基金No.2014CB542202the Natural Science Foundation of Jiangsu Province,China,No.BK20150409the Natural Science Foundation of Jiangsu Higher Education Institutions of China,No.15KJB180013the Scientific Research Foundation of Nantong University of China,No.14R29the Natural Science Foundation of Nantong City in China,No.MS12015043the Priority Academic Program Development of Jiangsu Higher Education Institutions of China
文摘Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, wheth- er tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/ threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in periph- eral nerve repair and regeneration.
文摘Statistic and typical-year composition methods are used to study the northwest Pacific typhoonactivities in relation with the El Nino and La Nina events. The result indicates that the typhoon tends to be inactive in the El Nino years and active in the La Nina years and it is also dependent on the onset and ending time andintensity of the events and areas of genesis of typhoons. With statistic features of the frequency of typhoon activity in the EI Nino and La Nina years and the time-lag correlation between the frequency and sea surface temperature (SST), useful information is provided for the prediction of typhoon occurrence. In addition, the singularvalues disassemble (SVD) method is applied to study the correlation between the geopotential field and SST fief(l.The result shows that the air-sea coupling in the EI Nino years is unfavorable for the typhoon to develop. whichtake place with a smaller number. Opposite situations are found with the La Nina years.
基金the Research Foundation of China(L2019027)Liaoning Revitalization Talents Program(XLYC1907166)the Deanship of Scientific Research(DSR)at King Abdulaziz University,Jeddah(KEP-2-135-39)。
文摘It is well-recognized that obsolete or discarded products can cause serious environmental pollution if they are poorly be handled.They contain reusable resource that can be recycled and used to generate desired economic benefits.Therefore,performing their efficient disassembly is highly important in green manufacturing and sustainable economic development.Their typical examples are electronic appliances and electromechanical/mechanical products.This paper presents a survey on the state of the art of disassembly sequence planning.It can help new researchers or decision makers to search for the right solution for optimal disassembly planning.It reviews the disassembly theory and methods that are applied for the processing,repair,and maintenance of obsolete/discarded products.This paper discusses the recent progress of disassembly sequencing planning in four major aspects:product disassembly modeling methods,mathematical programming methods,artificial intelligence methods,and uncertainty handling.This survey should stimulate readers to be engaged in the research,development and applications of disassembly and remanufacturing methodologies in the Industry 4.0 era.
基金This project is supported by National Natural Science Foundation of China (No.59990470-2).
文摘The cooperative work between human being and computer based on virtual reality (VR) is investigated to plan the disassembly sequences more efficiently. A three-layer model of human-computer cooperative virtual disassembly is built, and the corresponding human-computer system for stable virtual disassembly is developed. In this system, an immersive and interactive virtual disassembly environment has been created to provide planners with a more visual working scene. For cooperative disassembly, an intelligent module of stability analysis of disassembly operations is embedded into the human-computer system to assist planners to implement disassembly tasks better. The supporting matrix for stability analysis of disassembly operations is defined and the method of stability analysis is detailed. Based on the approach, the stability of any disassembly operation can be analyzed to instruct the manual virtual disassembly. At last, a disassembly case in the virtual environment is given to prove the validity of above ideas.
文摘Maintainability is an important design characterist ic , and also is a design parameter. The essence of maintainability design is to ex pect and solve possible maintenance problems at early stage during product devel opment, maintainability must be designed into product simultaneously. But curren t maintainability methods do not support concurrent engineering. This paper anal yzes the deficiencies of existing maintainability technologies, and introduces t he concept of maintainability analysis visualization. The two major deficiencies are that maintainability analysis time lags behind product design, and the main tainability techniques do not accommodate modern product design concepts. After this, the infrastructure of maintainability analysis visualization system is dis cussed. The system are composed of four subsystems, they are product modeling, d isassembly process modeling, simulation and analysis, and user interface. Produc t modeling is accomplished with the capability of CAD system, disassembly proces s modeling is mainly a reason process based on assembly information. The system is integrated into AutoCAD so that designer can timely analyze their product des ign from the viewpoint of easy maintenance. Human model generation function, hum an task analysis function, tool analysis function, visibility analysis function is implemented, disassembly process planning function is still under developing.
文摘The end-of-life vehicle recycling was studied based on the disassembly. The end-of-life recycling and the disassembly were reviewed and discussed. A disassembly experiment of an end-of-life engine was carried out, which strictly recorded the process of dismantling. Based on the results, a model of the end-of-life recycling was presented. In this model, the end-of-life parts were classified by three ways which included to recycle directly, to recycle after remanufacturing and to discard. By using this model, the dismantling efficiency and the recycling rate can be improved. Also, it obtains a good result after used in a dismantling factory.
基金The authors are grateful to the Raytheon Chair for Systems Engineering for funding.
文摘The evolution of Industry 4.0 made it essential to adopt the Internet of Things(IoT)and Cloud Computing(CC)technologies to perform activities in the new age of manufacturing.These technologies enable collecting,storing,and retrieving essential information from the manufacturing stage.Data collected at sites are shared with others where execution automatedly occurs.The obtained information must be validated at manufacturing to avoid undesirable data losses during the de-manufacturing process.However,information sharing from the assembly level at the manufacturing stage to disassembly at the product end-of-life state is a major concern.The current research validates the information optimally to offer a minimum set of activities to complete the disassembly process.An optimal disassembly sequence plan(DSP)can possess valid information to organize the necessary actions in manufacturing.However,finding an optimal DSP is complex because of its combinatorial nature.The genetic algorithm(GA)is a widely preferred artificial intelligence(AI)algorithm to obtain a near-optimal solution for the DSP problem.The converging nature at local optima is a limitation in the traditional GA.This study improvised the GA workability by integrating with the proposed priori crossover operator.An optimality function is defined to reduce disassembly effort by considering directional changes as parameters.The enhanced GA method is tested on a real-time product to evaluate the performance.The obtained results reveal that diversity control depends on the operators employed in the disassembly attributes.The proposed method’s solution can be stored in the cloud and shared through IoT devices for effective resource allocation and disassembly for maximum recovery of the product.The effectiveness of the proposed enhanced GA method is determined by making a comparative assessment with traditional GA and other AI methods at different population sizes.
基金National Natural Science Foundation of China(Grant Nos.52205526,52205529)Basic and Applied Basic Research Project of the Guangzhou Basic Research Program of China(Grant No.202201010284)+6 种基金National Foreign Expert Project of the Ministry of Science and Technology of China(Grant No.G2021199026L)National Key Research and Development Program of China(Grant Nos.2021YFB3301701,2021YFB3301702)Guangdong Provincial Graduate Education Innovation Program of China(Grant No.82620516)Guangzhou Municipal Innovation Leading Team Project of China(Grant No.201909010006)Guangdong Provincial"Quality Engineering"Construction Project of China(Grant No.210308)Guangdong Provincial Basic and Applied Basic Research Foundation of China(Grant No.2019A1515110399)Fundamental Research Funds for the Central Universities of China(Grant No.21620360).
文摘The recycling and remanufacturing of end-of-life products are significant for environmental protection and resource conservation.Disassembly is an essential process of remanufacturing end-of-life products.Effective disassembly plans help improve disassembly efficiency and reduce disassembly costs.This paper studies a disassembly planning problem with operation attributes,in which an integrated decision of the disassembly sequence,disassembly directions,and disassembly tools are made.Besides,a mathematical model is formulated with the objective of minimizing the penalty cost caused by the changing of operation attributes.Then,a neighborhood modularization-based artificial bee colony algorithm is developed,which contains a modular optimized design.Finally,two case studies with different scales and complexities are used to verify the performance of the proposed approach,and experimental results show that the proposed algorithm outperforms the two existing methods within an acceptable computational time.
基金This work was supported by the Frontier Interdisciplinary Innovation Fund from Fujian Institute of Research on the Structure of Matter, and Hundred Talents Project of Chinese Academy of Sciences
文摘Sarl is a small GTPase involved in COPⅡ vesicle transport. Previous studies showed that H79G mutation of Sarl can lock Sarl in its GTP-bound active conformation, stabilize coat assembly, and prevent the disassembly of COP Ⅱ vesicle coats by reducing Sec23/24 GAP- stimulated hydrolysis. We show here that the replacement of His79 by glycine induces a large conformation change in switch Ⅱ and results in the lost of hydrogen bond between His79 and its associated nucleophilic water molecule that was hypothesized to reduce the GAP-stimulated hydrolysis during the COP Ⅱ assembly and disassembly. These results confirm that the switch Ⅱ conformation is important for COP Ⅱ disassembly through coat-controlled GTP hydrolysis.
基金supported by grants from NIH CA-125707 NSF CBET-0729091 (C D ),as well as NIH AI-065566 (A A )
文摘Introduction Vascular endothelial (VE)-cadherin is localized to the endothelial borders and the adherens junctions,which are regulated by changes in mitogen activated protein kinases (MAPK),GTPases,and intracellular calcium. We previously
文摘When using shape memory materials into active disassembly of actual electronic products, because the elastic modulus of shape memory materials is affected by the temperature is relatively large, therefore, the main difference of environmental reliability between active disassembly products and common products is the impact of collision and vibration under different temperature. Establishing three-dimensional analysis model, comparing the impact of collision and vibration of mobile phone shells which are made up of PVC materials after casting & radiation and PC/ABS materials under different temperature. Analyzing the reliability of mobile phone under different temperature and optimizing its structure according to data of testing.
文摘In order to help designers, consider disassembly in their design activities, a method for evaluation of product disassembly is proposed. Criteria characterizing the disassembility of a product are identified. Each criterion is assigned a coefficient, thus proposing an index of easy fixations (Iff) as a design indicator to evaluate the level of disassembly of a solution generated by the designer. This index is calculated as a weighted average of the indicators for evaluating the disassembility of the product. The proposed method uses information such as the list of subsets or parts, the component tree, the part geometry, the functional links between components and parts, the properties of the components. It has been tested on one product: the soy roaster.
基金supported by the Foundation of Jiangsu Province for Talented Personnel and the Self-determined Research Program of Jiangnan University
文摘Challenges still remain in carrying out assembly modeling efficiently in virtual assembly (VA) fields. One of the root causes is the apparent weakness in effective description of assembly knowledge and information. The assembly modeling, disassembly modeling, assembly interference inspection, assembly sequence planning and optimization, and assembly simulation display for key techniques is studied theoretically in this paper. An example of product assembly modeling is provided to illustrate the effectiveness of the proposed approach. On the basis of re- search, using assembly simulation techniques and multimedia techniques to finish structure design in linkage design of a large size wind-drive generator. The application of the modeling method has shortened the lead time dramatically.