VOLUMETRIC-SWEPT DISPLAY SYSTEM BASED ON HELIX ROTATING SCREEN AND DMD

Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an imaging space meliorate the defects, such as the smaller image space, the fewer voxels and the severer voxel overlap dead zone caused by planar rotating screen. DMD with spatial light modular （SLM） technology increases the transmission bandwidth of 3-D data in the voxel activation subsystem and activate multi-voxel once time. The volumetric-swept system based on helix rotating screen and DMD is developed. The experimental results show that the image space, the vision dead zone, the voxels on slice, and the voxel activation capacity of the designed proto are superior to the plane rotating screen system.

Verifying the Effects of an Education Program Leveraging Information Technology to Promote Cervical Cancer Screening in Women Aged 20 - 29—A One-Year Longitudinal Study

The purpose of this research is to implement an IT-based education program in order to promote cervical cancer screenings for women aged 20 - 29 years, as well as to examine the results of said program. This is a longitudinal/comparative study of two groups, one for which the program was implemented (the intervention group), and the other for which it was not (the control group). The program consisted of attending a health lecture and encouragement to be screened one month, six months, and one year later sent through IT-based methods. The target was unmarried women aged 20 - 29 who had neither previously given birth nor had been screened for cervical cancer in a period one year prior. They were divided into two groups, the intervention group (n = 142) and control group (n = 145). The effectiveness of the program was assessed via an initial survey and further surveys six months and one year later. Results were based on the Japanese version of the Health Belief Model Scale for Cervical Cancer and the Pap Smear Test (HBMSCCPST), knowledge scores in the categories of Healthy Lifestyles, Cervical Cancer, Cervical Cancer Screening, and screening behavior. A two-way ANOVA of the HBMSCCPST subscales and knowledge scores in the initial, six-month, and one-year surveys was performed, showing interaction in Cervical Cancer (p = 0.00). Main effects were observed in Cervical Cancer Screening (p = 0.00) and Healthy Lifestyles (p = 0.00). Regarding the amount of change from the initial survey, knowledge scores in the Cervical Cancer (p = 0.027) and Cervical Cancer Screening (p = 0.016) categories were significantly higher in the intervention group than in the control group. There was no significant difference in cervical cancer screening rates (p = 0.26) between the two groups. However, a small-degree effect size was observed for Benefits, Seriousness, and Susceptibility subscales in both examinees and non-examinees. Although the educational program of this study was effective in improving the knowledge of women in their twenties, there was little improvement in HBMSCCPST and it did not lead to the promotion of cervical cancer screening. In order to raise interest in cervical cancer screening, it is necessary to consider useful content to guide women to consult with healthcare professionals, a long-term population approach, and organizational structure of consultation.

CAS 132-2006 Standard for Flat Panel Display TV Set Installation Service Formulating and Enforcing Main Point Explanation and Analysis

CAS 132-2006 formulation background and work courses Flat panel display technology is maturing alongside the development of science and technology. The prices of flat panel display TV sets are becoming lower and lower. Flat panel display TV sets have begun entering the mainstream and are now the first choice of some consumers when making new TV purchases.Display TV Set Installation Service Point Explanation and AnalysisRepair, Retreat and Change Duty Stipulations. Peculiar use conditions, usage environments, and the practical circumstances of user consumption service requirements, etc., were also taken into account on the basis of extensive investigation, testing, and verification.……

Primer Screening on Germplasm Resources of Mallotus oblongiolus by ISSR Molecular Marker

[ Objective] To provide effective primers for the rapid and accurate ISSR analysis of the germplasm materials of Mallotus oblongiolus （Miq.） Muello-Arg.. [Method] The modified CTAB method was used in the extraction of the genomic DNA. 99 ISSR primers were used in the ISSR-PCR amplification for 20 germplasm materials from 10 populations in Hainan Island, so that some primers, which were suitable to all gerplasm materials of M. oblongiolu, could be selected. [ Result] 15 effective primers with characteristics of rich polymorphism, clear bands, and good repeatability were selected from 99 test primers. The 15 primers selected were used in the ISSR-PCR amplification for 66 germplasm materials of M. oblongiolus. From all of which the abundant and distinct DNA fingerprintings could be obtained. 286 DNA bands were obtained, and of which 231 bands were polymorphic, which amounted to 80.77% of the total bands amplified. And 19.1 bands could be obtained with each primer, averagely. [ Conclusion] The 15 primers selected could be effectively applied to ISSR analysis of the germplasm resources of M. oblongiolus.

S-rough sets and knowledge separation

The conceptions of the knowledge screen generated by S-rough sets are given: f- screen and - screen , and then puts forward - filter theorem, - filter theorem of knowledge. At last, the applications of knowledge separation are given according to - screen and - screen.

Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp. ECU0401

By using acetonitrile as the sole nitrogen source, a microbial strain with high nitrilase activity, named as Alcaligenes sp. ECU0401, was newly isolated from soil, which could enantioselectively transform racemic mandelonitrile into (R)-(?)-mandelic acid, with an enantiomeric excess of >99.9%.

Detection of pancreatic cancer with normal carbohydrate antigen 19-9 using protein chip technology

AIM: To develop a method to differentiate pancreatic cancer patients from healthy or benign individuals when carbohydrate antigen (CA) 19-9 is normal.

Circulating miR-627-5p and miR-199a-5p are promising diagnostic biomarkers of colorectal neoplasia

BACKGROUND Early detection of colorectal neoplasms,including colorectal cancers(CRCs)and advanced colorectal adenomas(AAs),is crucial to improve patient survival.Circulating microRNAs(miRNAs)in peripheral blood are emerging as noninvasive diagnostic markers for multiple cancers,but their potential for screening colorectal neoplasms remains ambiguous.AIM To identify candidate circulating cell-free miRNAs as diagnostic biomarkers in patients with colorectal neoplasms.METHODS The study was divided into three phases:(1)Candidate miRNAs were selected from three public miRNA datasets using differential gene expression analysis methods;(2)an independent set of serum samples from 60 CRC patients,60 AA patients and 30 healthy controls(HCs)was included and analyzed by quantitative real-time polymerase chain reaction for miRNAs,and their diagnostic power was detected by receiver operating characteristic(ROC)analysis;and(3)the origin and function of miRNAs in cancer patients were investigated in cancer cell lines and tumor tissues.RESULTS Based on bioinformatics analysis,miR-627-5p and miR-199a-5p were differentially expressed in both the serum and tissues of patients with colorectal neoplasms and HCs and were selected for further study.Further validation in an independent cohort revealed that both circulating miR-627-5p and miR-199a-5p were sequentially increased from HCs and AAs to CRCs.The diagnostic power of miR-672-5p yielded an area under the curve(AUC)value of 0.90,and miR-199a-5p had an AUC of 0.83 in discriminating colorectal neoplasms from HCs.A logistic integrated model combining miR-199a-5p and miR-627-5p exhibited a higher diagnostic performance than either miRNA.Additionally,the levels of serum miR-627-5p and miR-199a-5p in CRC patients were significantly lower after surgery than before surgery and the expression of both miRNAs was increased with culture time in the culture media of several CRC cell lines,suggesting that the upregulated serum expression of both miRNAs in CRC might be tumor derived.Furthermore,in vitro experiments revealed that miR-627-5p and miR-199a-5p acted as tumor suppressors in CRC cells.CONCLUSION Serum levels of miR-199a-5p and miR-627-5p were markedly increased in patients with colorectal neoplasms and showed strong potential as minimally invasive biomarkers for the early screening of colorectal neoplasms.

Development of a Zebrafish Model for Rapid Drug Screening against Alzheimer＇s Disease

Alzheimer＇s disease, the leading cause of dementia in the elderly, is a complex neurodegenerative disorder which leads to a progressive decline in cognitive functions. A rapid screening model is highly demanded for identification and evaluation of novel anti-Alzheimer＇s disease drugs from a large numbers of compounds. Until now, numerous studies utilized zebrafish model for drug discovery. Since aluminum can induce a similar biological activity in zebrafish as in Alzheimer patients, in this study, we developed a novel animal model using 3 to 5 day post-fertilization larval zebrafish by optimizing the doses and duration of aluminum chloride exposure. Six anti-Alzheimer＇s disease drugs with a variety of mechanisms were tested to validate the newly developed zebrafish model. Importantly, Rivastigmine, ThT, Flurbiprofen and AM-117 could increase the value of Dyskinesia Recovery Rate by 53.4-64%, 169.4-200%, 54.5-96% and 70.9-121%, respectively. Rivastigmine, Memantine, ThT, Flurbiprofen, Rosiglitazone and AM-117 improved the value of Response Efficiency by 86.6-175.1%, 28.2-66.6%, 127.2-236.5%, 118.3-323.7%, 26.6-140.8% and 70.2-161.4%, respectively. Our results suggest that the zebrafish model developed in this study could be a useful tool for high throughput screening of potential novel anti-Alzheimer＇s disease leading compounds targeting acetylcholinesterase, N-methyl-D-aspartic acid receptor, γ-secretase, peroxisome proliferator-activated receptor-γand amyloid-β.

Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon a2b

Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhIFNa2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNa2b.RhIFNa2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNa2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNa2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNa2b detection assay had a limit of detection of 1 mg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other commercial products,with a great potential for routine use in detecting counterfeit recombinant protein products.

Construction of a Multipurpose M13KE Phage Display System

In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput screening. Based on the relationship between the structure and function of minor coat protein of wild-type MI3KE （wt-plII）, a truncated gene III （tglll） encoding minor coat protein from M13KE phage was cloned. A fusion gene fragment harboring a hw/tac promoter, signal peptide and C-terminal region sequence of gill was assembled with SOEing-PCR （splice-overlapping-extension polymerase chain reaction） method and inserted into M13KE vector. SDS-PAGE and Western blot analysis with anti-M13 pIII moneclonal antibody were employed to detect the expression of re- combinant protein, c-Myc and HA tag sequences were fused into the recombinant protein. The results showed that tglll was inserted into an unessential region of M13KE. According to the results of SDS-PAGE and Western blot with anti-M13 pIII antibody, pIII was expressed by wt-gIII and tgIII, glII harboring two tags ex- pressed both c-Myc and HA peptides using SDS-PAGE and Western blot with the corresponding monoclonal antibodies. In this study, a multipurpose M13KE phage display system was successfully constructed, which could express both short and long peptide libraries without helper phage. In future, the obtained M13KE phage display system may be used for targeted high-throughput screening of long peptide libraries without helper phage.

Liquid crystal Fresnel lens display

A novel see-through display with a liquid crystal lens array was proposed.A liquid crystal Fresnel lens display（LCFLD） with a holographic screen was demonstrated.The proposed display system has high efficiency,simple fabrication,and low manufacturing cost due to the absence of a polarizer and color filter.

Information Visualization Theorem for Battlefield Screen

Designing military screen (display) is not complicated in the past, the design should contains easiness of using, visibility and functionality. And it is important that contains all of the information what the system could be gotten. Today military display design can be valued by consideration of human’s cognition model. A few information, which operators of military display could easily miss, could bring critical loss. So the military display needs the ability to help operator decide the military decision with sufficient consideration of battlefield information in a short time. The information is considerably complicated because it has many military entities and many attributes for each entities. For this reason this research contains the methodology of information organization especially for visual information. When the operator use military display, the required information should be recognized at a glance and located where the operator can place his eye easily. Therefore, the display screen should efficiently organize and effectively transfer the information. This paper specifically proposes design methodology to effectively organize and visualize the information in the display screen. The theory of information visualization in a variety of fields already exist, but it has no established theory for the origin of the display screen design. Since the theory was selected to organize the military display interface design for the display screen of the battlefield situation existing information visualization theory.

User Centered Design for Battlefield Screen

This thesis paper is a brief summary of user centered design, (particularly in the situation of under the battlefield), which incorporates the related information after reviewing many other papers. A display screen user requires an ability to provide proper information while considering a variety of information in a short period. While operating the display screen, the user is heavily pressed for time as the missions are allocated through many different steps of orders. In addition, since a failure would cause fatal damage or cause further defeat, this increases the pressure of time. The right environment for operating a display screen is most useful when the user is in the most vulnerable time period or long-term continuous operation of the display screen. In that kind of sterile environment, the operating display screen is there to help the user’s decisions for the matter at hand, in this it should follow the user centered design and improve the user’s handiness and use. Furthermore, the information should be provided in accordance with the user's informational and perceptional process, and the capacity of the information process should be improved with the movement through each process and step. This thesis paper can be used as a reference material when designing the display screen in order to apply a user’s experiences.

Pre-process algorithm for satellite laser ranging data based on curve recognition from points cloud

The satellite laser ranging （SLR） data quality from the COMPASS was analyzed, and the difference between curve recognition in computer vision and pre-process of SLR data finally proposed a new algorithm for SLR was discussed data based on curve recognition from points cloud is proposed. The results obtained by the new algorithm are 85 % （or even higher） consistent with that of the screen displaying method, furthermore, the new method can process SLR data automatically, which makes it possible to be used in the development of the COMPASS navigation system.

Encoding and display technologies for combinatorial libraries in drug discovery:The coming of age from biology to therapy

Drug discovery is a sophisticated process that incorporates scientific innovations and cuttingedge technologies.Compared to traditional bioactivity-based screening methods,encoding and display technologies for combinatorial libraries have recently advanced from proof-of-principle experiments to promising tools for pharmaceutical hit discovery due to their high screening efficiency,throughput,and resource minimization.This review systematically summarizes the development history,typology,and prospective applications of encoding and displayed technologies,including phage display,ribosomal display,mRNA display,yeast cell display,one-bead one-compound,DNA-encoded,peptide nucleic acidencoded,and new peptide-encoded technologies,and examples of preclinical and clinical translation.We discuss the progress of novel targeted therapeutic agents,covering a spectrum from small-molecule inhibitors and nonpeptidic macrocycles to linear,monocyclic,and bicyclic peptides,in addition to antibodies.We also address the pending challenges and future prospects of drug discovery,including the size of screening libraries,advantages and disadvantages of the technology,clinical translational potential,and market space.This review is intended to establish a comprehensive high-throughput drug discovery strategy for scientific researchers and clinical drug developers.

数字视频ASIC中OSD系统的实现

介绍1款视频处理ASIC芯片中OSD系统的设计方案,先读取OSD内部SRAM中的属性和字符内容,然后控制电路根据属性将字符内容以RGB的形式表现出来,最后将OSD信息与视频内容进行α混合,从而可以在屏幕上得到OSD信息。该OSD系统用户自定义性强,使用改进的内部SRAM中1/2/4像素深度(BPP,Bits Per Pixel)字符或图形点阵的存储结构以提高字符或图形存储量,其具有闪烁、阴影、透明、单个字符宽高加倍、非线性放大、可编程的行列数和颜色等诸多功能。芯片在UMC0.18μm CMOS工艺下流片,其OSD系统的工作频率可达170 MHz。

Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor

Hepatitis B immunoglobulin （HBIG） is important in the management of hepatitis B virus （HBV） infection. Aiming to develop recombinant monoclona# antibodies as an alternative to HBIG, we report the successful identification of HBV surface antigen （HBsAg）-specific antibodies from a full-length human antibody library displayed on mammalian cell surface. Using total RNA of peripheral blood mononuclear cells of a natively immunized donor as template, the antibody repertoire was amplified. Combining four-way ligation and the FIp recombinase-mediated integration （FIp-ln） system, we constructed a mammalian cell-based, fully human, full-length antibody display library in which each cell displayed only one kind of antibody molecule. By screening the cell library using fluorescence-activated cell sorting （FACS）, eight cell clones that displayed HBsAg-specific antibodies on cell surfaces were identified. DNA sequence analysis of the antibody genes revealed three unique antibodies. FACS data indicated that fluorescent strength of expression （FSE）, fluorescent strength of binding （FSB） and relative binding ability （RBA） were all different among them. These results demonstrated that by using our antibody mammalian display and screening platform, we can successfully identify antigen-specific antibodies from an immunized full-length antibody library. Therefore, this platform is very useful for the development of therapeutic antibodies.

Immersive autostereoscopic display based on curved screen and parallax barrier

In this Letter,we present a display system based on a curved screen and parallax barrier,which provides stereo images with a horizontal field of view of 360°without wearing any eyewear,to achieve an immersive autostereoscopic effect.The display principle and characteristics of this display system are studied theoretically in detail.Three consecutive pixels on a curved screen and parallax barrier form a display unit,which can generate separate viewing zones for the left and right eyes,respectively.Simulation and experimental results show that the non-crosstalk effect can be obtained in the viewing zones,which proves the effectiveness of this display system.This study provides some new ideas for the improvement of the autostereoscopic display and to enable envisioned applications in virtual reality technology.

360° light field 3D display system based on a triplet lenses array and holographic functional screen

A 360° light field 3D display system is presented, which consists of a liquid crystal display, a novel triplet lenses array, and a holographic functional screen （HFS）. The mapping relationship among pixels, 3D objects, and view- ing positions are investigated. The aberration analysis of the single lens is carried out both in the simulation and the experiment, which shows that it cannot provide an excellent 3D image to the viewers. In order to suppress the aberrations, ＂the primary aberration theory＂ and ＂the damped least-squares method＂ are used for optical analysis and lens design. A 3D image with aberration correction can be viewed around the proposed display system.