Abstract： Daqu distiller＇s grains were co-fermented as raw materials by Trichoderma.koningii and Trichosporon cutaneum to produce microbial oils, which can provide raw materials for bio-diesel development. The singl...Abstract： Daqu distiller＇s grains were co-fermented as raw materials by Trichoderma.koningii and Trichosporon cutaneum to produce microbial oils, which can provide raw materials for bio-diesel development. The single factor tests were used to investigate the effects of ratio of strains, inoculum size, culture temperature and culture time on the production of microbial oils. The best processing conditions were obtained by orthogonal test through measuring the content of microbial oils in product. The result shows that the microbial oils content of the co-fermented product under the ratio of Trichoderma.koningii to Trichosporon cutaneum of 1：1, the inoculum size of strains of 11% at 28 ℃ for 6 d is 7.15 g/L. It is shown that the production of microbial oils co-fermented by Trichoderma.koningii and Trichosporon cutaneum with Daqu distiller＇s grains is possible. The research provides a new idea for the reuse of Daqu distiller＇s grains and also provides a new way for the development of microbial oils.展开更多
Pot experiments were conducted to study the effects of different organic fertilizers made from distiller＇s grains on the physiological characteristics and growth of flue-cured tobacco. The results showed that the agr...Pot experiments were conducted to study the effects of different organic fertilizers made from distiller＇s grains on the physiological characteristics and growth of flue-cured tobacco. The results showed that the agronomic traits and biomass of flue-cured tobacco in the three treatments were higher than that of the control group,and the mixed application of different organic fertilizers made from distiller＇s grains and compound fertilizer could promote the growth and development of flue-cured tobacco significantly.展开更多
In order to reduce the use of peat resources and realize the sustainable development of tobacco, the pH of distiller s grain substrate was adjusted with humic acid, and the effects of various distiller s grain substra...In order to reduce the use of peat resources and realize the sustainable development of tobacco, the pH of distiller s grain substrate was adjusted with humic acid, and the effects of various distiller s grain substrates on the growth of tobacco seedlings were studied to find out the method of cultivating high-quality tobacco seedlings by using distiller s grain substrate. The results showed that when 60% of humic acid was added to the distiller s grain substrate made from coarse distiller s grains, fine distiller s grains, perlite and vermiculite, both the pH and electrical conductivity decreased significantly and were similar to that of the conventional substrate. Moreover, the emergence rate of tobacco seedlings, the rate of strong tobacco seedlings, leaf number, stem height, root growth and quality of tobacco seedlings were good.展开更多
Brewer’s spent grain(BSG)-a byproduct generated from the processing of beer-brewing industry-is currently recognized as an important source of bioactive peptides with potential health benefits.In previous studies we ...Brewer’s spent grain(BSG)-a byproduct generated from the processing of beer-brewing industry-is currently recognized as an important source of bioactive peptides with potential health benefits.In previous studies we demonstrated that peptides obtained from BSG(BSG-P)can exert in vitro biofunctional activities including hypolipidemic effects.However,there are no studies about the effects of gastrointestinal digestion on BSG lipid-lowering peptides and it is unknown whether the bioactive properties observed in vitro remain in vivo.The aims of this work were to evaluate the microencapsulation as strategy to preserve hypolipidemic BSG-P under a simulated gastrointestinal digestion,and the bioavailability of microencapsulated BSG-P using a rodent model of hyperlipidemia and other metabolic-related disorders induced by the consumption of a high-sucrose diet.In vitro studies showed that microencapsulation improved the BSG-P reduced hypolipidemic activity observed under a simulated gastrointestinal digestion by increasing the inhibition of the fat digestion enzymes,pancreatic lipase and cholesterol esterase.Furthermore,in vivo studies demonstrated that microencapsulated BSG-P were also able to reduce the increased serum lipid levels(triglycerides-TG-cholesterol and free fatty acids),energy intake,visceral adipocytes size and TG content observed in sucrose-rich diet-fed rats.This was accompanied by reduced liver lipids levels and the activities of key enzymes involved in dietary fat absorption.Therefore,our findings show that microencapsulation is a useful strategy to preserve the hypolipidemic activity of BSG-P and support the concept that microencapsulated BSG-P would be an interesting and cheaper natural source to prevent hyperlipidemias and its related-disorders.展开更多
基金Project(10A110) supported by Hunan Provincial Education Department of ChinaProject(2010JT4055) supported by Hunan Provincial Science & Technology Department of China
文摘Abstract： Daqu distiller＇s grains were co-fermented as raw materials by Trichoderma.koningii and Trichosporon cutaneum to produce microbial oils, which can provide raw materials for bio-diesel development. The single factor tests were used to investigate the effects of ratio of strains, inoculum size, culture temperature and culture time on the production of microbial oils. The best processing conditions were obtained by orthogonal test through measuring the content of microbial oils in product. The result shows that the microbial oils content of the co-fermented product under the ratio of Trichoderma.koningii to Trichosporon cutaneum of 1：1, the inoculum size of strains of 11% at 28 ℃ for 6 d is 7.15 g/L. It is shown that the production of microbial oils co-fermented by Trichoderma.koningii and Trichosporon cutaneum with Daqu distiller＇s grains is possible. The research provides a new idea for the reuse of Daqu distiller＇s grains and also provides a new way for the development of microbial oils.
基金Supported by the Project of Luzhou Company of Sichuan Provincial Tobacco Company"Comprehensive Application of Distillers'Grains on Tobacco"
文摘Pot experiments were conducted to study the effects of different organic fertilizers made from distiller＇s grains on the physiological characteristics and growth of flue-cured tobacco. The results showed that the agronomic traits and biomass of flue-cured tobacco in the three treatments were higher than that of the control group,and the mixed application of different organic fertilizers made from distiller＇s grains and compound fertilizer could promote the growth and development of flue-cured tobacco significantly.
基金Supported by the Project of Luzhou Company of Sichuan Provincial Tobacco Company"Comprehensive Application of Distillers'Grains on Tobacco"
文摘In order to reduce the use of peat resources and realize the sustainable development of tobacco, the pH of distiller s grain substrate was adjusted with humic acid, and the effects of various distiller s grain substrates on the growth of tobacco seedlings were studied to find out the method of cultivating high-quality tobacco seedlings by using distiller s grain substrate. The results showed that when 60% of humic acid was added to the distiller s grain substrate made from coarse distiller s grains, fine distiller s grains, perlite and vermiculite, both the pH and electrical conductivity decreased significantly and were similar to that of the conventional substrate. Moreover, the emergence rate of tobacco seedlings, the rate of strong tobacco seedlings, leaf number, stem height, root growth and quality of tobacco seedlings were good.
基金supported by the Agencia Santafesina de Ciencia,Tecnología e Innovaci´on from Argentine for the financial support(Project Investigaci´on Orientada 2018-IO-2018-00046).
文摘Brewer’s spent grain(BSG)-a byproduct generated from the processing of beer-brewing industry-is currently recognized as an important source of bioactive peptides with potential health benefits.In previous studies we demonstrated that peptides obtained from BSG(BSG-P)can exert in vitro biofunctional activities including hypolipidemic effects.However,there are no studies about the effects of gastrointestinal digestion on BSG lipid-lowering peptides and it is unknown whether the bioactive properties observed in vitro remain in vivo.The aims of this work were to evaluate the microencapsulation as strategy to preserve hypolipidemic BSG-P under a simulated gastrointestinal digestion,and the bioavailability of microencapsulated BSG-P using a rodent model of hyperlipidemia and other metabolic-related disorders induced by the consumption of a high-sucrose diet.In vitro studies showed that microencapsulation improved the BSG-P reduced hypolipidemic activity observed under a simulated gastrointestinal digestion by increasing the inhibition of the fat digestion enzymes,pancreatic lipase and cholesterol esterase.Furthermore,in vivo studies demonstrated that microencapsulated BSG-P were also able to reduce the increased serum lipid levels(triglycerides-TG-cholesterol and free fatty acids),energy intake,visceral adipocytes size and TG content observed in sucrose-rich diet-fed rats.This was accompanied by reduced liver lipids levels and the activities of key enzymes involved in dietary fat absorption.Therefore,our findings show that microencapsulation is a useful strategy to preserve the hypolipidemic activity of BSG-P and support the concept that microencapsulated BSG-P would be an interesting and cheaper natural source to prevent hyperlipidemias and its related-disorders.
