A possible probe to neutron-skin thickness by fragment parallel momentum distribution in projectile fragmentation reactions

Neutron-skin thickness is a key parameter for a neutron-rich nucleus;however,it is difficult to determine.In the framework of the Lanzhou Quantum Molecular Dynamics(LQMD)model,a possible probe for the neutron-skin thickness(δ_(np))of neutron-rich ^(48)Ca was studied in the 140A MeV ^(48)Ca+^(9)Be projectile fragmentation reaction based on the parallel momentum distribution(p∥)of the residual fragments.A Fermi-type density distribution was employed to initiate the neutron density distributions in the LQMD simulations.A combined Gaussian function with different width parameters for the left side(Γ_(L))and the right side(Γ_(R))in the distribution was used to describe the p∥of the residual fragments.Taking neutron-rich sulfur isotopes as examples,Γ_(L) shows a sensitive correlation withδ_(np) of ^(48)Ca,and is proposed as a probe for determining the neutron skin thickness of the projectile nucleus.

DISTRIBUTION FEATURES OF GIANT SEA－SALT NUCLEI IN ATMOSPHERE OVER YONGXING ISLAND， XISHA ISLANDS，DURING NORTHEAST WINTER MONSOON

In this paper, the chlorine ions nuclei data, observed at Yongxing (Xisha Islands ) during November 1987, are analyzed. The main results are: 1) The average concentration of chiorine ions nuclei with dry diameters larger than 2 μm is 878 per litre and the average salt content is 105. 4μg/m3, in which special giant nuclei with dry diameters larger than 4 μm are 19O per litre. The largest dry diameter of chloride ions observed reaches 57 μm, which are larger than that observed there during the southwest summer monsoon. The spetrum type shows quasi-unimodal and exponential lapse. 2) The salt content decreases with the height over sea surface. 3) The concentrations of chlorine ions nuclei reach the maximum value at 0800 (Local time, same below ). and minimum at 0200.

Study of Isotopic Distribution of the Projectile-like Fragments Produced in the ^(17,18)N + ^(197)Au Reactions at 33 MeV/u

The experimental data of the isotopic distribution for projectile-like fragments are presented for the 17,18N ＋ 197Au reaction at 33 MeV/u. The width of the isotopic distributions for lSN projectile is significantly broader than that for 17N projectile, and the average N/Z ratio of the former shifts to higher neutron number side. As long as the realistic nucleon density distribution is used, the isotopic distribution for fragments is reproduced by the simple abrasion-ablation model calculation, which thus provides an independent way to determine the surface distribution of the nuclear matter density for neutron-rich nuclei.

Induction of apoptosis by TPA and VP-16 is through translocation of TR3

AIM: To investigate the role of TR3 in induction of apoptosis in gastric cancer cells. METHODS: Human gastric cancer cell line, MGC80-3, was used. Expression of TR3 mRNA and its protein was detected by Northern blot and Western blot. Localization of TR3 protein was showed by immunofluorescence analysis under laser-scanning confocal microscope. Apoptotic morphology was observed by DAPI fluorescence staining, and apoptotic index was counted among 1000 cells randomly. Stable transfection assay was carried out by Lipofectamine. RESULTS: Treatment of MGC80-3 cells with TPA and VP-16 resulted in apoptosis, accompanied by the repression of Bcl-2 protein in a time-dependent manner. At the same time, TPA and VP-16 also up-regulated expression level of TR3 mRNA in MGC80-3 cells that expressed TR3 mRNA. When antisense-TR3 expression vector was transfected into the cells, expression of TR3 protein was repressed. In this case, TPA and VP-16 did not induce apoptosis. In addition, TPA and VP-16-induced apoptosis involved in translocation of TR3. In MGC80-3 cells, TR3 localized concentrative in nucleus, after treatment of cells with TPA and VP-16, TR3 translocated from nucleus to cytosol obviously. However, when this nuclear translocation was blocked by LMB, apoptosis was not occurred in MGC80-3 cells even in the presence of TPA and VP-16. CONCLUSION: Induction of apoptosis by TPA and VP-16 is through induction of TR3 expression and translocation of TR3 from nucleus to cytosol, which may be a novel signal pathway for TR3, and represent the new biological function of TR3 to exert its effect on apoptosis in gastric cancer cells.

结直肠癌HMGB2蛋白不同核质分布的临床病理意义

目的探讨HMGB2蛋白在结直肠癌(CRC)细胞内的不同表达分布与患者临床病理参数及预后的关系。方法选取江西省人民医院2016至2020年间135例CRC根治标本存档蜡块及患者临床资料为研究对象,采用免疫组化法检测CRC组织中HMGB2蛋白表达分布,分析其与患者临床病理参数及预后的关系。结果HMGB2蛋白分布于CRC细胞核和细胞质中,其在细胞核中的表达分布与结直肠癌组织的低分化、深浸润、伴随淋巴结转移和/或远处转移、TNM分期晚密切相关,而胞质表达则与之相反(P<0.05)。HMGB2蛋白胞核表达的CRC患者总生存期及五年生存率显著低于胞质表达以及胞质和胞核同时表达的患者(P<0.05)。结论HMGB2蛋白胞核表达可能有助于CRC进展和预后不良。

Zbed3 participates in the subcortical maternal complex and regulates the distribution of organelles

We previously identified a subcortical maternal complex （SCMC） that is essential for early embryogenesis and female fertility in mice. However, the molecular mechanism by which the SCMC affects female fertility remains largely uncharacterized. Here, we report that a novel maternal protein, zinc finger BED-type containing 3 （Zbed3）, participates in the SCMC. Depletion of maternal Zbed3 results in reduced fecundity of females, because of the impaired and delayed development in a proportion of mutant embryos. The loss of maternal Zbed3 results in asymmetric zygotic division and abnormal distributions of organeUes in the affected oocytes and zygotes, similar to the phenotypes observed in females with disrupted core SCMC genes. Further investiga- tion revealed that these phenotypes are associated with disrupted dynamics of microtubules and/or formation of cytoplasmic lat- tices （CPLs）. The stability and localization of Zbed3 depend on, but are not required for, the formation of the SCMC. Thus, our data suggest Zbed3 as one of downstream proteins mediating SCMC functions and provide further insights into the roles of the SCMC and CPLs in female fertility.

Isospin Dependence of Fragmentation Cross Sections in Collisions of Neutron-Rich Ca Isotopes with ^(12)C

The cross sections of fragments （δf） produced in the even 36～52Ca projectile frag- mentation reactions at 80 AMeV are calculated in the framework of the statistical abrasion- ablation model. The distributions of af are investigated. Linear correlations between the peak positions of the distributions and charge numbers of the fragment isotopes are found.

Energy Level Statistics of SO（5） Limit of Super-symmetry U（6/4） in Interacting Boson-Fermion Model

We study the energy level statistics of the SO(5) limit of super-symmetry U(6/4) in odd-A nucleus using the interacting boson-fermion model. The nearest neighbor spacing distribution (NSD) and the spectral rigidity (△3)are investigated, and the factors that affect the properties of level statistics are also discussed. The results show that the boson number N is a dominant factor. If N is small, both the interaction strengths of subgroups SOB(5) and SOBF(5)and the spin play important roles in the energy level statistics, however, along with the increase of N, the statistics distribution would tend to be in Poisson form.

ULTRASTRUCTURAL EVIDENCE OF THE CYTO PLASMIC ASSEMBLY OF NUCLEOCAPSIDS OF HERPES SIMPLEX VIRUS TYPES 1 AND 2 BYTHIN SECTION AND IMMUNOFERRITIN ELECTRON MICROSCOPY

There is general agreement that herpesvirus nucleocapsid assembly occurs in the nucleus of infected cells.However, we have seen ultrastructural evidence suggestive of concurrent cytoplasmic assembly ofherpes simplex virions by bothroutine and immunoferritin electron microscopy.Complete and incomplete particles-resembling nucleocapsids formed in the nucleus were observedin the cytoplasm.

Langevin study of neutron emission in the reactions ^(16)O+^(181)Ta and ^(19)F+^(178)Hf

The pre-scission neutrons measured in the reactions ^16O＋^181Ta and ^19F＋^178Hf are studied via a Langevin equation coupled with a statistical decay model. We find that because of the mass asymmetry of different entrance channels, the spin distributions of compound nuclei would be different, consequently, the measured neutrons in these two reactions would also different. This means that the entrance channel will affect the particle emission in the fission process of hot nuclei.

粘类小麦CMS育性基因分布区研究

为更有效地选育小麦不育系的高恢复系,以8个粘类小麦细胞质雄性不育系(Cytoplasmic malesterility,CMS)为测验种,对310份来自国内外不同地区的普通小麦品种及亲本材料对粘类小麦CMS系的育性恢保关系进行调查,并采用聚类分析法研究了粘类小麦CMS育性基因的地区分布。结果表明:(1)高恢复能力的品种在中国春麦区和南方冬麦区的分布比例均超过50%,具有保持性的品种在加拿大分布最多,我国北方冬麦区次之,春麦区分布最少。(2)不同地区品种恢复度存在差异。(3)将48个普通品种聚为3类:第1类品种对粘类CMS的平均恢复度均低于20%,属全不育和高不育类型;第2类品种的平均恢复度在20%～55%范围内,基本属于半不育类型;第3类品种的平均恢复度均大于55%,属高可育和全可育类型。(4)对不育系进行聚类:90-110核背景下,K型和V型聚为一类,Ven型和B型聚为一类;224核型下V型和B型聚为一类,再和K型聚为一类,与Ven型距离最远;相同细胞质背景下,90-110和224两种核型分别在一定水平上被分为2个类别。

水稻细胞质雄性不育系裂颖种子的穗上分布规律研究

对4个具有不同裂颖特性的水稻细胞质雄性不育系及其保持系的种子裂颖率和4个不育系种子裂颖粒在穗上的分布特点进行了研究。结果表明:保持系种子裂颖轻于不育系,不育系与相应保持系的种子裂颖率呈显著正相关。不育系种子裂颖与同穗颖花开放的迟早有关,开花迟,裂颖率高;强势穗裂颖轻于弱势穗;一次枝梗轻于二次枝梗;穗上部轻于中部,中部轻于下部;一次枝梗第6粒位裂颖最轻,第2粒位相对较重;强势粒轻于弱势粒。

抗病毒基因HERC5新型环状RNA hsa＿circ＿0001426的特征分析及鉴定

目的旨在研究来源于抗病毒基因HERC5的新型环状RNA hsa_circ_0001426的特征,并探索该环状RNA真核过表达载体的构建和鉴定。方法从反向剪切位点的验证、蛋白翻译的潜能、对RNase R处理的抵抗性、在细胞内的胞质胞核分布情况四个方面来研究hsa_circ_0001426的特征。此外,还利用环状RNA克隆构建试剂盒来构建hsa_circ_0001426的过表达载体,并验证其表达效果。结果 hsa_circ_0001426是由HERC5基因第18号外显子反向剪切至第12号外显子形成,其序列中包含一条可翻译318个氨基酸的开放阅读框,其抵抗Rnase R的处理,主要分布于细胞质。针对该环状RNA的过表达载体构建成功,其在转染HEK293T细胞后48 h的表达量最高。结论首次成功分析了抗病毒基因HERC5的新型环状RNA hsa_circ_0001426的特征,并利用试剂盒首次成功构建了hsa_circ_0001426的真核过表达载体,并可在细胞中高效表达;为深入研究hsa_circ_0001426的特征和功能奠定基础,并进一步扩充了HERC5基因的相关研究。

低温种植下水稻F_2群体生物学性状的遗传分析

以耐冷性极强的粳稻老品种小麻谷与具有籼稻细胞质背景的改良粳稻品种南34为亲本,将正反交杂合体在4个海拔产生的F2群体种植在2200m海拔点对其生物学性状进行遗传分析.结果表明,两亲本在低温下种植其生物学性状存在极为明显的差异,而正反交F1的差异则主要表现在穗长和结实率上;在不同海拔产生的正反交F2群体中生物学性状的变异系数、遗传率、遗传进度、偏度、蜂度等遗传参数的表现存在极为明显的差异.经次数分布的卡方检验表明,所有性状均显著或极显著偏离正态分布;其中,在所有F2群体中穗长和结实率均偏向小麻谷,然而有效穗数、1～2穗节长、穗颈长、总粒数和着粒密度均偏向南34;株高在F2群体中的偏向受细胞质背景和产生F2群体海拔的影响.此外,F2群体的形态多样性和遗传分化明显受到细胞质和海拔的影响.该研究结果对探讨环境温度对细胞质效应的影响具有重要的参考价值.

凝血酶引致的血小板内钙、镁离子浓度及分布状态的变化

我们使用荧光探针ｆｕｒａ２、ｍａｇ－ｆｕｒａ２和ｆｌｕｏ３测定了凝血酶引致的血小板凝聚过程中细胞内钙、镁离子浓度的变化及分布状态。在０．５Ｕ／ｍｌ凝血酶作用下，血小板细胞内钙离子浓度呈双时相变化。血小板细胞群中细胞内钙离子浓度呈正态分布。伴随血小板凝聚时细胞内钙离子浓度增加，血小板细胞内游离镁离子浓度也明显增加，提示镁离子在血小板凝聚中有重要的作用。

血液常规分析衍生炎性参数指标在抗中性粒细胞胞浆抗体相关性血管炎中的临床意义分析

目的探讨血液常规分析衍生炎性参数指标[中性粒细胞/淋巴细胞比值(NLR)、血小板/淋巴细胞比值(PLR)、系统性免疫性炎症指数(SII)、中性粒细胞、单核细胞乘积与淋巴细胞比值(NMLR)、红细胞分布宽度(RDW)]在抗中性粒细胞胞浆抗体相关性血管炎(anti-neutrophil cytoplasmic antibody associated vasculitis,AAV)中的临床意义。方法选择2017年1月至2021年10月我院初诊的52例AAV患者为研究对象,50例健康体检人群为对照组,分析AAV患者血液常规分析衍生炎性参数NLR、PLR、SII、NMLR、RDW及其他血液炎性指标的变化及相关性。结果AAV患者SII、NLR、PLR、NMLR以及RDW均显著高于健康对照组(P<0.01),PDW结果与健康对照组比较差异无统计学意义(P>0.05);Spearman相关性分析显示,AAV患者NMLR与PA(r=-0.3589,P=0.009)呈负相关,PLR与ESR(r=0.3910,P=0.0042)和α1球蛋白(r=0.3715,P=0.0067)呈正相关,与PA(r=-0.3231,P=0.0195)呈负相关;SII水平与α1球蛋白(r=0.4520,P=0.0008)、CRP(r=0.5014,P=0.0002)、ESR(r=0.3188,P=0.0212)呈正相关,与PA(r=-0.4801,P=0.0003)呈负相关;RDW水平与α1球蛋白(r=0.3306,P=0.0167)、NLR(r=0.3238,P=0.0192)、NMLR(r=0.3945,P=0.0038)呈正相关。结论以循环血液中细胞数量、细胞体积异质性为核心衍生的NLR、SII、NMLR、PLR、RDW等参数是一种简便、易行的炎症参数,在反映AAV疾病活动方面具有潜在的临床应用前景,有望为AAV患者的临床管理提供有价值的帮助。

石英晶体谐振器频率测量的误差因素分析

在使用通用的阻抗计法测量石英晶体谐振器的谐振频率和其它电参数时，晶体接入测量线路时产生的分布参数严重影响其测量精度．分析这些影响产生的原因并据此改进测量方法和采取适当的补偿措施。

育龄夫妇支原体感染分布情况及药敏分析

目的:探究育龄夫妇支原体感染分布情况并分析其药敏情况。方法:2010年1月至2015年1月期间共有3644对夫妇来我院进行支原体筛查,采用珠海迪尔生物工程公司生产的支原体培养、鉴定和药敏试剂盒对接受支原体筛查的3644对夫妇进行支原体培养和鉴定,并给予支原体阳性者抗生素的药敏试验。结果:3644对夫妇中共有447例支原体阳性者,支原体阳性率为6.13%。其中单独丈夫阳性5例(1.12%),单独妻子阳性4例(0.89%),夫妻同时阳性438例(97.99%)。447例支原体阳性者中有14例(3.13%)人型支原体(MH),324例(72.48%)解脲支原体(Uu)和109(24.38%)例两种支原体混合感染者。多西环素和交沙霉素对人型支原体和解脲支原体最敏感,其次是美满霉素。人型支原体耐药率最高的是克拉霉素、红霉素和阿奇霉素,其次是环丙沙星和罗红霉素。解脲支原体耐药率最高的是环丙沙星,其次是红霉素和氧氟沙星。结论:临床上经验性的使用克拉霉素、红霉素、阿奇霉素治疗支原体感染并不能达到较佳的临床疗效,药物治疗应当结合药敏检验进行。

NUTRITIONAL LIPID LIVER DISEASE OF GRASS CARP CTENOPHARYNGODON IDULLUS(C.et V.)

The inadequate nutrient content of pellet feeds widely used in recent years in China for grass carp forming led to lipid liver degeneration in the fish. The present studies show that the pathological features of lipid liver disease are anaemia and hepatic ceroidosis. Other clinical features are: the ratio of liver to body weight exceeds 3% and lipid content exceeds 5%. Extreme infiltration of hepaiocytes by lipid results in the following deteriorative effects: swelling of the liver cells, increase of lipid droplets in the cytoplasm and dislocation of the nucleus, loss of cytoplasm staining affinity, and increased activities of GOT and GPT in serum. Lipid liver degeneration of grass carp can be divided into three stages: 1) deposition of liver lipid; 2) lipid infiltration of hepatic parenchyma; 3) atrophy of liver nucleus. The causes of lipid liver degeneration are complicated, but the main cause is assumed to be an imbalance of nutrients in daily feed and the lack of some lipotropic substances.

CYTOPLASMIC EFFECT OF Xenopus laevis ON NUCLEAR FUNCTION OF ITS ALBINO MUTANT

The nucleo-cytoplasmic interactions have been studied by many investigators and it is well established that nucleus and cytoplasm are interdependent and interacting. We have studied the effects of the cytoplasm of wild-type Xenopus