This paper reported the diurnal variations of photochemical efficiency of PSII, thermaldissipation rate and other physiology process in the low content chlorophyll mutant rice andits wild type under field conditions, ...This paper reported the diurnal variations of photochemical efficiency of PSII, thermaldissipation rate and other physiology process in the low content chlorophyll mutant rice andits wild type under field conditions, and analyzed the difference of absorbed light distributionbetween the two rice varieties in a day. The results showed that the mutant had poor absorbedlight because of its little light absorption coefficient, but higher electron transportg ratecould partly reduce the disadvantageous effect of deficient absorbed light in mutant. Comparedwith wild-type rice, the mutant had less excess excitation energy and the fraction of absorbedlight allocated to photochemical process was more.展开更多
Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Exami...Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Examining with SDS-PTGE and liquid nitrogen temperature fluorescence measurement showed that they contained only PSII and light-harvesting pigment-protein complexes (LHCP),and there was no detectable amount of PSI. Freeze-fracture electromicroscopic observation confirmed that this part was really an appressed lamella membrane. Through divalent cation Mg^(++), the thylakoid membranes were induced to unstack and restack.With the addition of Mg^(++), the fluorescence intensity was changed instantly. We realized that there existed two processes:One was a rapid process which was accomplished within 30 s. The other was a slow process of which the time duration was about 60 min. This dual effects of Mg^(++) had not been reported before.We had analyzed the change of F685/F730 and discussed the possible rneehanis ms of light energy distribution between photosystems.展开更多
基金supported by the National Natural Science Foundation of China(30300217).
文摘This paper reported the diurnal variations of photochemical efficiency of PSII, thermaldissipation rate and other physiology process in the low content chlorophyll mutant rice andits wild type under field conditions, and analyzed the difference of absorbed light distributionbetween the two rice varieties in a day. The results showed that the mutant had poor absorbedlight because of its little light absorption coefficient, but higher electron transportg ratecould partly reduce the disadvantageous effect of deficient absorbed light in mutant. Comparedwith wild-type rice, the mutant had less excess excitation energy and the fraction of absorbedlight allocated to photochemical process was more.
文摘Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Examining with SDS-PTGE and liquid nitrogen temperature fluorescence measurement showed that they contained only PSII and light-harvesting pigment-protein complexes (LHCP),and there was no detectable amount of PSI. Freeze-fracture electromicroscopic observation confirmed that this part was really an appressed lamella membrane. Through divalent cation Mg^(++), the thylakoid membranes were induced to unstack and restack.With the addition of Mg^(++), the fluorescence intensity was changed instantly. We realized that there existed two processes:One was a rapid process which was accomplished within 30 s. The other was a slow process of which the time duration was about 60 min. This dual effects of Mg^(++) had not been reported before.We had analyzed the change of F685/F730 and discussed the possible rneehanis ms of light energy distribution between photosystems.
