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佛肚竹枯萎病的研究 被引量:1
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作者 宋漳 林毓银 《林业科学》 EI CAS CSCD 北大核心 2001年第z1期181-184,共4页
The wilt disease of Bambusa ventricosa was a serious disease occured in Nanping city, Fujian Province. The symptoms of the disease, identification, inoculation, biological characteristics of the causal organism and bi... The wilt disease of Bambusa ventricosa was a serious disease occured in Nanping city, Fujian Province. The symptoms of the disease, identification, inoculation, biological characteristics of the causal organism and bionomics of the wilt disease were studied. The species was identified as Nectria ditissima Tul. at sexual phase, and as Fusarium semitectum Berk. & Rav. at anamorphic state. The causal organism of wilt disease grew vigorously on the PSA and PSA+10% decoction of culm media, and formed white coloured colonies. After incubation of 7 days at 28℃, a great amount of macroconidia was produced, no stroma and perithecia were formed after 30 days. The stroma and ascospore were only produced under natural condition. The preference temperature for mycelia growth was between 25~28℃, and the optimum pH values was at 6~7. The optimum temperature for conidia and ascospores germination was between 25~28℃, and the optimum pH values was at 6~7. The high relative humidity (RH 96.1%~100%) was necessary for the germination of conidia and ascospores. The results of experiments showed that the pathogen of the wilt disease overwintered on the soil, and disseminated by winds and rains to infect the wounded stem base of Bambusa ventricosa. The primary infection occurred from the last ten days of February or the first days of March. The peak infection occurred from the first ten days of March to the second ten days of April during early summer rains. The infection ceased from the second ten days or the last ten days of May. The freeze injury was possibly important factor to induce infection of the wilt disease. 展开更多
关键词 佛肚竹 枯萎病 鲜红从赤壳菌 半裸镰刀菌
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Weaver Ant, Oecophylla Ionginoda (Latreille) (Hymenoptera: Formicidae) Activity Reduces Fruit Fly Damage in Citrus Orchards
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作者 Isaac Newton Ativor Kwame Afreh-Nuamah +1 位作者 Maxwell Kelvin Billah Daniel Obeng-Ofori 《Journal of Agricultural Science and Technology(A)》 2012年第4期449-458,共10页
Fruit flies are causing extensive socio-economic losses in citrus orchards in Ghana. The flies as quarantine pests have detrimental effects on the export market due to international trade regulations. Oecophylla speci... Fruit flies are causing extensive socio-economic losses in citrus orchards in Ghana. The flies as quarantine pests have detrimental effects on the export market due to international trade regulations. Oecophylla species have been tested as biological control agents on other crops, and have shown great potential in reducing the effects of pests. The effectiveness of Oecophylla as a biocontrol agent of fruit flies was compared with insecticide, Cypermethrin + Dimethoate (Cydim super~) at Forest and Horticultural Crops Research Centre, Kade, in the Eastern Region of Ghana. The results have shown that trees colonized by Oecophylla had between 6%-10% fly infestation and Cypermethrin + Dimethoate (1614 mg a.i. mL~ tree"1) recorded 3.0% infestation. Cypermethrin + Dimethoate treatment recorded 3.50 ~ 0.87 dropped fruits, and Oecophylla colonized-trees recorded 15 to 19.20 -4- 3.61. Oecophylla colonized-trees recorded yield values from 12.90 to 15.20 tons ha-~ whilst Cypermethrin + Dimethoate treatment recorded 27.90 tons hat fruit yield. Total number of fly landings in the presence of Oecophylla (72.00) was significantly lower than the absence of Oecophylla (114.20). Set-up with no Oecophylla recorded the highest infestation index (71.17) while the presence recorded 45.83. These results suggest that Oecophylla longinoda can be used as a biocontrol agent for IPM programs in citrus orchards. 展开更多
关键词 Oecophylla longinoda biological control fruit flies Ceratitis ditissima CITRUS Ghana.
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进境新西兰苹果中欧洲枝溃疡病菌的分离和鉴定
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作者 焦彬彬 马纯珏 +2 位作者 何善勇 孙涛 杨翠云 《植物检疫》 2021年第2期33-38,共6页
从新西兰进境苹果的褐腐组织中分离得到1株新丛赤壳属菌株3072,对该菌株进行形态学特征观察、PCR检测、序列比对分析和致病性测定。结果表明,在PDA培养基上菌落乳白色至黄白色,气生菌丝茂密,边缘规则;在MEA培养基上菌落为白色、平铺生... 从新西兰进境苹果的褐腐组织中分离得到1株新丛赤壳属菌株3072,对该菌株进行形态学特征观察、PCR检测、序列比对分析和致病性测定。结果表明,在PDA培养基上菌落乳白色至黄白色,气生菌丝茂密,边缘规则;在MEA培养基上菌落为白色、平铺生长。菌株为异宗配合。利用欧洲枝溃疡病菌(Neonectria ditissima)特异检测引物对Bt-fw135/Bt-rw284扩增菌株的基因组DNA得到150 bp的预期目标条带。菌株的β-tubulin序列与GenBank中N.ditissima的序列相似性为99.43%100%。基于β-tubulin序列构建的系统进化树显示菌株3072与N.ditissima聚在同一个分支,亲缘关系最近。菌株接种苹果,接种处组织14 d后出现典型褐腐症状。根据上述检测结果,将菌株3072鉴定为欧洲枝溃疡病菌(N.ditissima)。 展开更多
关键词 苹果 欧洲枝溃疡病菌 鉴定
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