The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring ...The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring two hormones that show daily oscillations, cortisol and ACTH, in serum using ELISA kits. The levels of the two hormones were highest at 8:00 am and their rhythmic changes were similar to those in humans. In addition, the influence of stress of handling and blood collection on the physiological parameters of the gerbils was examined. After adaptation to handling for 1 week, some serum parameters in the animals changed. Handling and blood collection did not impact significantly on the following parameters: creatine kinase (CK), lactate dehydrogenase (LD), alanine aminotransferase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and albumin (ALB). However, blood glucose (GLU), total protein (TP) and globulin (GLB) significantly increased while creatinine (CRE) and albumin/globulin (A/G) significantly decreased after adaptation. This work further confirms that the Mongolian gerbil is a diurnal animal and also indicates that a suitable adaptation procedure is necessary for getting reliable results when performing experiments using these animals.展开更多
Gut microbiota is generally recognized to play a crucial role in maintaining host health and metabolism.The correlation among gut microbiota,glycolipid metabolism,and metabolic diseases has been well reviewed in human...Gut microbiota is generally recognized to play a crucial role in maintaining host health and metabolism.The correlation among gut microbiota,glycolipid metabolism,and metabolic diseases has been well reviewed in humans.However,the interplay between gut microbiota and host metabolism in swine remains incompletely understood.Given the limitation in conducting human experiments and the high similarity between swine and humans in terms of anatomy,physiology,polyphagy,habits,and metabolism and in terms of the composition of gut microbiota,there is a pressing need to summarize the knowledge gained regarding swine gut microbiota,its interplay with host metabolism,and the underlying mechanisms.This review aimed to outline the bidirectional regulation between gut microbiota and nutrient metabolism in swine and to emphasize the action mechanisms underlying the complex microbiomeehost crosstalk via the gut microbiotaegutebrain axis.Moreover,it highlights the new advances in knowledge of the diurnal rhythmicity of gut microbiota.A better understanding of these aspects can not only shed light on healthy and efficient pork production but also promote our knowledge on the associations between gut microbiota and the microbiomeehost crosstalk mechanism.More importantly,knowledge on microbiota,host health and metabolism facilitates the development of a precise intervention therapy targeting the gut microbiota.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:No.31572341
文摘The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring two hormones that show daily oscillations, cortisol and ACTH, in serum using ELISA kits. The levels of the two hormones were highest at 8:00 am and their rhythmic changes were similar to those in humans. In addition, the influence of stress of handling and blood collection on the physiological parameters of the gerbils was examined. After adaptation to handling for 1 week, some serum parameters in the animals changed. Handling and blood collection did not impact significantly on the following parameters: creatine kinase (CK), lactate dehydrogenase (LD), alanine aminotransferase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and albumin (ALB). However, blood glucose (GLU), total protein (TP) and globulin (GLB) significantly increased while creatinine (CRE) and albumin/globulin (A/G) significantly decreased after adaptation. This work further confirms that the Mongolian gerbil is a diurnal animal and also indicates that a suitable adaptation procedure is necessary for getting reliable results when performing experiments using these animals.
基金supported by the National Natural Science Foundation of China(3187130113)the National Key R&D Program of China(2018YFD0500404)
文摘Gut microbiota is generally recognized to play a crucial role in maintaining host health and metabolism.The correlation among gut microbiota,glycolipid metabolism,and metabolic diseases has been well reviewed in humans.However,the interplay between gut microbiota and host metabolism in swine remains incompletely understood.Given the limitation in conducting human experiments and the high similarity between swine and humans in terms of anatomy,physiology,polyphagy,habits,and metabolism and in terms of the composition of gut microbiota,there is a pressing need to summarize the knowledge gained regarding swine gut microbiota,its interplay with host metabolism,and the underlying mechanisms.This review aimed to outline the bidirectional regulation between gut microbiota and nutrient metabolism in swine and to emphasize the action mechanisms underlying the complex microbiomeehost crosstalk via the gut microbiotaegutebrain axis.Moreover,it highlights the new advances in knowledge of the diurnal rhythmicity of gut microbiota.A better understanding of these aspects can not only shed light on healthy and efficient pork production but also promote our knowledge on the associations between gut microbiota and the microbiomeehost crosstalk mechanism.More importantly,knowledge on microbiota,host health and metabolism facilitates the development of a precise intervention therapy targeting the gut microbiota.
