Extensive studies have been performed to describe the phenotypic changes occurring during malignant transformation of the prostate.However,the cell types and associated changes that contribute to the development of pr...Extensive studies have been performed to describe the phenotypic changes occurring during malignant transformation of the prostate.However,the cell types and associated changes that contribute to the development of prostate diseases and cancer remain elusive,largely due to the heterogeneous composition of prostatic tissues.Here,we conduct a comprehensive evaluation of four human prostate tissues by singlecell RNA sequencing(sc RNA-seq)to analyze their cellular compositions.We identify 18 clusters of cell types,each with distinct gene expression profiles and unique features;of these,one cluster of epithelial cells(Ep)is found to be associated with immune function.In addition,we characterize a special cluster of fibroblasts and aberrant signaling changes associated with prostate cancer(PCa).Moreover,we provide insights into the epithelial changes that occur during the cellular senescence and aging.These results expand our understanding of the unique functional associations between the diverse prostatic cell types and the contributions of specific cell clusters to the malignant transformation of prostate tissues and PCa development.展开更多
Amphibia ns play a key role in structuring biological assemblages of agricultural landscapes,but they are threatened by global agricultural intensification.Landscape structure is an important variable in flue ncing bi...Amphibia ns play a key role in structuring biological assemblages of agricultural landscapes,but they are threatened by global agricultural intensification.Landscape structure is an important variable in flue ncing biodiversity in agricultural la ndscapes.However,in the Yangtze River Delta,where a"farmland-orchard-fishpond"agricultural pattern is common,the effects of landscape construction on anuran populations are unclear.In this study,we examined the effects of agricultural landscape parameters on the abundance and body condition of the rice frog(Fejervarya multistriata),which is a dominant anuran species in farmland in China.Employing a visual encounter method,we surveyed rice frog abundance for 3years across 20 agricultural landscapes.We also calculated the body condition index(BCD of 188 male frog individuals from these agricultural landscapes.Landscape variables,comprising landscape compositional heterogeneity(using the Shannon diversity in dex of all land cover types except buildings and roads),Ian dscape con figurational heterogeneity(using landscape edge density),breeding habitat diversity(using the number of 5 waterbody types available as breeding habitats),and areas of forest were also measured for each 1-km radius landscape.We found that the amount of forest in each agricultural Iandscape had a sign讦icant positive relationship with rice frog abundance,and breeding habitat diversity was positively related to the BCI of male rice frogs.However,body condition was negatively impacted by landscape configurational heterogeneity.Our results suggested the importance of nonagricultural habitats in agricultural landscapes,such as waterbodies and forest,to benefit rice frog population persistenee.展开更多
基金funded by the Youth Science Foundation of Guangxi Medical University(GXMUYSF201810)the National Natural Science Foundation of China(81901538,81670750,81770759)+3 种基金the Sigrid Juse’lius Foundation and the Recruitment Fund of Fudan Universitythe National Key Research and Development Program of China(2017YFC0908000)the Major Project of Guangxi Innovation Driven(AA18118016)the Guangxi Key Laboratory for Genomic and Personalized Medicine(16-380-54,17-259-45,19-050-22,19-185-33,and 20-065-33)。
文摘Extensive studies have been performed to describe the phenotypic changes occurring during malignant transformation of the prostate.However,the cell types and associated changes that contribute to the development of prostate diseases and cancer remain elusive,largely due to the heterogeneous composition of prostatic tissues.Here,we conduct a comprehensive evaluation of four human prostate tissues by singlecell RNA sequencing(sc RNA-seq)to analyze their cellular compositions.We identify 18 clusters of cell types,each with distinct gene expression profiles and unique features;of these,one cluster of epithelial cells(Ep)is found to be associated with immune function.In addition,we characterize a special cluster of fibroblasts and aberrant signaling changes associated with prostate cancer(PCa).Moreover,we provide insights into the epithelial changes that occur during the cellular senescence and aging.These results expand our understanding of the unique functional associations between the diverse prostatic cell types and the contributions of specific cell clusters to the malignant transformation of prostate tissues and PCa development.
基金This work was supported financially by the Natural Science Foundation of China(No.31901099,31800350)Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration(No.SHUES2019B03)+2 种基金Science and Technology Commission of Shanghai Municipality(No.18DZ1205000)Institute of Eco-Chongming(No.ECNU-IEC-201902)Shanghai Landscaping and City Appearance Administrative Bureau(No.F131508).
文摘Amphibia ns play a key role in structuring biological assemblages of agricultural landscapes,but they are threatened by global agricultural intensification.Landscape structure is an important variable in flue ncing biodiversity in agricultural la ndscapes.However,in the Yangtze River Delta,where a"farmland-orchard-fishpond"agricultural pattern is common,the effects of landscape construction on anuran populations are unclear.In this study,we examined the effects of agricultural landscape parameters on the abundance and body condition of the rice frog(Fejervarya multistriata),which is a dominant anuran species in farmland in China.Employing a visual encounter method,we surveyed rice frog abundance for 3years across 20 agricultural landscapes.We also calculated the body condition index(BCD of 188 male frog individuals from these agricultural landscapes.Landscape variables,comprising landscape compositional heterogeneity(using the Shannon diversity in dex of all land cover types except buildings and roads),Ian dscape con figurational heterogeneity(using landscape edge density),breeding habitat diversity(using the number of 5 waterbody types available as breeding habitats),and areas of forest were also measured for each 1-km radius landscape.We found that the amount of forest in each agricultural Iandscape had a sign讦icant positive relationship with rice frog abundance,and breeding habitat diversity was positively related to the BCI of male rice frogs.However,body condition was negatively impacted by landscape configurational heterogeneity.Our results suggested the importance of nonagricultural habitats in agricultural landscapes,such as waterbodies and forest,to benefit rice frog population persistenee.