As large-scale genomic studies have progressed,it has been revealed that a single reference genome pattern cannot represent genetic diversity at the species level.While domestic animals tend to have complex routes of ...As large-scale genomic studies have progressed,it has been revealed that a single reference genome pattern cannot represent genetic diversity at the species level.While domestic animals tend to have complex routes of origin and migration,suggesting a possible omission of some population-specific sequences in the current reference genome.Conversely,the pangenome is a collection of all DNA sequences of a species that contains sequences shared by all individuals(core genome)and is also able to display sequence information unique to each individual(variable genome).The progress of pangenome research in humans,plants and domestic animals has proved that the missing genetic components and the identification of large structural variants(SVs)can be explored through pangenomic studies.Many individual specific sequences have been shown to be related to biological adaptability,phenotype and important economic traits.The maturity of technologies and methods such as third-generation sequencing,Tel-omere-to-telomere genomes,graphic genomes,and reference-free assembly will further promote the development of pangenome.In the future,pangenome combined with long-read data and multi-omics will help to resolve large SVs and their relationship with the main economic traits of interest in domesticated animals,providing better insights into animal domestication,evolution and breeding.In this review,we mainly discuss how pangenome analysis reveals genetic variations in domestic animals(sheep,cattle,pigs,chickens)and their impacts on phenotypes and how this can contribute to the understanding of species diversity.Additionally,we also go through potential issues and the future perspectives of pangenome research in livestock and poultry.展开更多
Management strategies and the use of advanced technologies are equally important for determining the sample number and sampling frame for successful field sampling for animal disease prevalence studies.The quality of ...Management strategies and the use of advanced technologies are equally important for determining the sample number and sampling frame for successful field sampling for animal disease prevalence studies.The quality of the biological samples collected in the field has a direct bearing on the integrity of the data generated,prevalence estimates and subsequent policy decisions on disease control.Hence,compromising the quality of biological samples collected in the field could potentially undermine the priority setting principles in disease control strategies.Biological samples collected from domestic animals in the field are precious materials and require meticulous planning for sample collection,sample storage in the field,transportation,and storage in the laboratory.Poorly managed field sampling has a significant detrimental impact on the sample quality and quantity and directly affects the accuracy of disease prevalence data.A bad choice of sampling tools,containers,storage and transport all have a negative impact on the integrity of the sample and consequently have an impact on the outcome.Over the last two years,as part of our one health animal sampling work in ndia,we have observed challenges and opportunities in the field sampling of animals for disease prevalence studies.This paper aims to provide information on management practices and technologies for efficient biological sample collection from the field and ensure that good quality samples are available fortesting.展开更多
Purpose The paper shows the interpretive impact of different constructions of the point of view available to the reader/viewer in book and animated movie versions of a children's picture book,a novel for pre-adole...Purpose The paper shows the interpretive impact of different constructions of the point of view available to the reader/viewer in book and animated movie versions of a children's picture book,a novel for pre-adolescents/early teenagers,and a graphic novel for adolescents and adults.Design/Approach/Methods Excerpts from book and animated movie versions of the same story are compared using multimodal analysis of interpersonal meaning to show how the reader/viewer is positioned in relation to the characters in each version,complemented by analyses of ideational meaning to show the effect of point of view on interpretive possibilities.Findings Focusing mainly on multimodal construction of point of view,the analyses show how interpretive possibilities of ostensibly the same story are significantly reconfigured in animated adaptations compared with book versions even when the verbal narrative remains substantially unchanged.Originality/Value The study shows that it is crucial to students’critical appreciation of,and their creative contribution to,their evolving digital literary culture that in this new era of educational technology,attention in literacy and literary education focuses on developing understandings of digital multimodal narrative art,and that animated movie adaptations are not presented pedagogically as isomorphic with,or simply adjunct to,corresponding book versions.展开更多
China’s domestic animation industry is deeply rooted in its rich traditional cultural heritage.While continuously exploring and showcasing the unique charm of such cultural heritage through storytelling,imagery estab...China’s domestic animation industry is deeply rooted in its rich traditional cultural heritage.While continuously exploring and showcasing the unique charm of such cultural heritage through storytelling,imagery establishment,and spirit creation,Chinese animation also seamlessly integrates modern aesthetic characteristics and cultural values into its development within the context of the new era.By adopting a contemporary perspective,it innovatively expresses the essence of traditional culture while striving to shape and present a reliable,admirable and respectable image of China.Chinese animation aims to create credible contemporary animation images by drawing inspiration from traditional Chinese cultural archetypes.It also seeks to revitalize admirable traditional cultural imagery using vibrant and prevailing ACGN visuals,while shaping credible characters with richer cultural connotations and crafting stories with more enchanting plots to convey the spiritual essence of the Chinese nation.By establishing reliable,admirable and respectable animation images,the Chinese animation industry strives to enhance its capability to“tell China’s stories well and make the voice of China heard,”to better promote the image of China in the new era from a global perspective.展开更多
基金supported by the National Natural Science Foundation of China (grant numbers 31961143021)the earmarked fund for Modern Agro-industry Technology Research System (grant numbers CARS-39-01)+1 种基金the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences (grant numbers ASTIP-IAS01) to YM and LJsupported by the Elite Youth Program in Chinese Academy of Agricultural Sciences
文摘As large-scale genomic studies have progressed,it has been revealed that a single reference genome pattern cannot represent genetic diversity at the species level.While domestic animals tend to have complex routes of origin and migration,suggesting a possible omission of some population-specific sequences in the current reference genome.Conversely,the pangenome is a collection of all DNA sequences of a species that contains sequences shared by all individuals(core genome)and is also able to display sequence information unique to each individual(variable genome).The progress of pangenome research in humans,plants and domestic animals has proved that the missing genetic components and the identification of large structural variants(SVs)can be explored through pangenomic studies.Many individual specific sequences have been shown to be related to biological adaptability,phenotype and important economic traits.The maturity of technologies and methods such as third-generation sequencing,Tel-omere-to-telomere genomes,graphic genomes,and reference-free assembly will further promote the development of pangenome.In the future,pangenome combined with long-read data and multi-omics will help to resolve large SVs and their relationship with the main economic traits of interest in domesticated animals,providing better insights into animal domestication,evolution and breeding.In this review,we mainly discuss how pangenome analysis reveals genetic variations in domestic animals(sheep,cattle,pigs,chickens)and their impacts on phenotypes and how this can contribute to the understanding of species diversity.Additionally,we also go through potential issues and the future perspectives of pangenome research in livestock and poultry.
基金supported by the Department of Biotechnology,Ministry of Science and Technology,Government of India(No.BT/PR39032/ADV/90/285/2020).
文摘Management strategies and the use of advanced technologies are equally important for determining the sample number and sampling frame for successful field sampling for animal disease prevalence studies.The quality of the biological samples collected in the field has a direct bearing on the integrity of the data generated,prevalence estimates and subsequent policy decisions on disease control.Hence,compromising the quality of biological samples collected in the field could potentially undermine the priority setting principles in disease control strategies.Biological samples collected from domestic animals in the field are precious materials and require meticulous planning for sample collection,sample storage in the field,transportation,and storage in the laboratory.Poorly managed field sampling has a significant detrimental impact on the sample quality and quantity and directly affects the accuracy of disease prevalence data.A bad choice of sampling tools,containers,storage and transport all have a negative impact on the integrity of the sample and consequently have an impact on the outcome.Over the last two years,as part of our one health animal sampling work in ndia,we have observed challenges and opportunities in the field sampling of animals for disease prevalence studies.This paper aims to provide information on management practices and technologies for efficient biological sample collection from the field and ensure that good quality samples are available fortesting.
文摘Purpose The paper shows the interpretive impact of different constructions of the point of view available to the reader/viewer in book and animated movie versions of a children's picture book,a novel for pre-adolescents/early teenagers,and a graphic novel for adolescents and adults.Design/Approach/Methods Excerpts from book and animated movie versions of the same story are compared using multimodal analysis of interpersonal meaning to show how the reader/viewer is positioned in relation to the characters in each version,complemented by analyses of ideational meaning to show the effect of point of view on interpretive possibilities.Findings Focusing mainly on multimodal construction of point of view,the analyses show how interpretive possibilities of ostensibly the same story are significantly reconfigured in animated adaptations compared with book versions even when the verbal narrative remains substantially unchanged.Originality/Value The study shows that it is crucial to students’critical appreciation of,and their creative contribution to,their evolving digital literary culture that in this new era of educational technology,attention in literacy and literary education focuses on developing understandings of digital multimodal narrative art,and that animated movie adaptations are not presented pedagogically as isomorphic with,or simply adjunct to,corresponding book versions.
文摘基因编辑是指对生物体基因组特定的DNA进行改造,使生物的性状发生定向的、可遗传的改变。基因编辑技术主要包括锌指核酸酶(Zinc Finger Nucleases,ZFNs)技术、转录激活因子样效应物核酸酶(Transcription Activator-Like Effector Nucleases,TALENs)技术、成簇规则间隔短回文重复序列/Cas9(Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated Protein 9,CRISPRs/Cas9)技术。在畜禽中使用高效且精确的基因编辑技术可以提高畜禽产量、品质、抗病力等。本文从基因编辑技术的发展、原理及其在畜禽遗传育种中的应用进行综述,为基因编辑技术应用于畜禽遗传育种的研究提供参考。
基金funded by “Multi-dimensional Reconstruction and Symbol Hybridity:Research on Innovative Expression of Traditional Culture in Domestic Animated Films”(WHCY2023B28)a project funded by the Cultural Industry Development Research Center of Sichuan Provincial Key Research Base of Philosophy and Social Sciences+1 种基金funded by “An Intertextual Analysis of Li Bing’s Story from a Cross-media Perspective”(RX2300002875)a research project funded by the Li Bing Research Center at the Sichuan Provincial Key Research Base of Philosophy and Social Sciences
文摘China’s domestic animation industry is deeply rooted in its rich traditional cultural heritage.While continuously exploring and showcasing the unique charm of such cultural heritage through storytelling,imagery establishment,and spirit creation,Chinese animation also seamlessly integrates modern aesthetic characteristics and cultural values into its development within the context of the new era.By adopting a contemporary perspective,it innovatively expresses the essence of traditional culture while striving to shape and present a reliable,admirable and respectable image of China.Chinese animation aims to create credible contemporary animation images by drawing inspiration from traditional Chinese cultural archetypes.It also seeks to revitalize admirable traditional cultural imagery using vibrant and prevailing ACGN visuals,while shaping credible characters with richer cultural connotations and crafting stories with more enchanting plots to convey the spiritual essence of the Chinese nation.By establishing reliable,admirable and respectable animation images,the Chinese animation industry strives to enhance its capability to“tell China’s stories well and make the voice of China heard,”to better promote the image of China in the new era from a global perspective.
