Since the first report of the release of insects carrying a dominant lethal （RIDL） strategy, RIDL strains have been constructed in species including fruit flies and mosquitoes. However, in many insects, identificati...Since the first report of the release of insects carrying a dominant lethal （RIDL） strategy, RIDL strains have been constructed in species including fruit flies and mosquitoes. However, in many insects, identification of sterile and lethal genes needed to generate a RIDL strain is limited by the lack of molecular and genetic information. Here, we created RIDL strains of Drosophila melanogaster using RNA interference （RNAi） of the Pygopus （Pygo） gene, a key component of the Wingless/Wnt signaling pathway. In two transgenic lines, XDll and XD15, we verified lethality in the absence of tetracycline, but we were unable to demonstrate sex-specific lethality. We found that male XD15 adults maintained on medium without tetracycline had a longer lifespan than wild type. This RNAibased RIDL strain may therefore offer the advantages of a transgene that promotes the expression of two contrary actions at different life stages： lethality in larvae and prolonged lifespan in adults, actions that could work together to provide prolonged delivery of lethality by the RIDL system. Use of RNAi can facilitate the development and application of RIDL strategies in a wide range of species.展开更多
The aim of this study was to obtain unusual mutations called conditional. The mutations manifest in some, not all representatives of a species. Collections of these mutations in chromosomes X, 2, and 3 of Drosophila m...The aim of this study was to obtain unusual mutations called conditional. The mutations manifest in some, not all representatives of a species. Collections of these mutations in chromosomes X, 2, and 3 of Drosophila melanogaster were established. Sex of fly or chromosomal rearrangement was the conditions providing ＂manifestation-non manifestation＂ of these mutations. The mutations differ from the usual by a set of properties. The salient differences in addition to conditional manifestation include： manifestation dependence on the spatial arrangement of chromosomal material in the genome, parental effects （maternal or paternal） of the mutant, capacity for transferring the genome from stable to unstable state. It is suggested that conditional mutations are mutant variants of Drosophila regulatory genes contained by the large Genomic Regulatory Network of Drosophila. Thus, the genes of this category can be detected by using special breeding procedures, mutations of these genes have unusual manifestation.展开更多
基金supported by the National Natural Science Foundation of China (30800721)
文摘Since the first report of the release of insects carrying a dominant lethal （RIDL） strategy, RIDL strains have been constructed in species including fruit flies and mosquitoes. However, in many insects, identification of sterile and lethal genes needed to generate a RIDL strain is limited by the lack of molecular and genetic information. Here, we created RIDL strains of Drosophila melanogaster using RNA interference （RNAi） of the Pygopus （Pygo） gene, a key component of the Wingless/Wnt signaling pathway. In two transgenic lines, XDll and XD15, we verified lethality in the absence of tetracycline, but we were unable to demonstrate sex-specific lethality. We found that male XD15 adults maintained on medium without tetracycline had a longer lifespan than wild type. This RNAibased RIDL strain may therefore offer the advantages of a transgene that promotes the expression of two contrary actions at different life stages： lethality in larvae and prolonged lifespan in adults, actions that could work together to provide prolonged delivery of lethality by the RIDL system. Use of RNAi can facilitate the development and application of RIDL strategies in a wide range of species.
文摘The aim of this study was to obtain unusual mutations called conditional. The mutations manifest in some, not all representatives of a species. Collections of these mutations in chromosomes X, 2, and 3 of Drosophila melanogaster were established. Sex of fly or chromosomal rearrangement was the conditions providing ＂manifestation-non manifestation＂ of these mutations. The mutations differ from the usual by a set of properties. The salient differences in addition to conditional manifestation include： manifestation dependence on the spatial arrangement of chromosomal material in the genome, parental effects （maternal or paternal） of the mutant, capacity for transferring the genome from stable to unstable state. It is suggested that conditional mutations are mutant variants of Drosophila regulatory genes contained by the large Genomic Regulatory Network of Drosophila. Thus, the genes of this category can be detected by using special breeding procedures, mutations of these genes have unusual manifestation.
